全文获取类型
收费全文 | 568282篇 |
免费 | 49843篇 |
国内免费 | 315篇 |
专业分类
618440篇 |
出版年
2018年 | 10064篇 |
2017年 | 9714篇 |
2016年 | 10108篇 |
2015年 | 8311篇 |
2014年 | 10088篇 |
2013年 | 14198篇 |
2012年 | 18163篇 |
2011年 | 22357篇 |
2010年 | 15990篇 |
2009年 | 14632篇 |
2008年 | 19477篇 |
2007年 | 21381篇 |
2006年 | 14888篇 |
2005年 | 14704篇 |
2004年 | 14459篇 |
2003年 | 14203篇 |
2002年 | 14178篇 |
2001年 | 25884篇 |
2000年 | 25907篇 |
1999年 | 19880篇 |
1998年 | 6026篇 |
1997年 | 6171篇 |
1996年 | 5829篇 |
1995年 | 5419篇 |
1994年 | 5297篇 |
1993年 | 5111篇 |
1992年 | 16027篇 |
1991年 | 15903篇 |
1990年 | 15414篇 |
1989年 | 15004篇 |
1988年 | 13973篇 |
1987年 | 13029篇 |
1986年 | 11879篇 |
1985年 | 12005篇 |
1984年 | 9578篇 |
1983年 | 8201篇 |
1982年 | 5872篇 |
1981年 | 5088篇 |
1980年 | 4863篇 |
1979年 | 9030篇 |
1978年 | 6822篇 |
1977年 | 6178篇 |
1976年 | 5807篇 |
1975年 | 6807篇 |
1974年 | 7236篇 |
1973年 | 7125篇 |
1972年 | 7038篇 |
1971年 | 6302篇 |
1970年 | 5160篇 |
1969年 | 4891篇 |
排序方式: 共有10000条查询结果,搜索用时 0 毫秒
111.
beta-lactamase has been purified to a homogeneous state from Mycobacterium butyricum ATCC 19979. The molecular weight (Mr = 29,000) and the isoelectric point (4,0) of the enzyme have been determined. The enzyme showed both penicillinase and cephalosporinase activity, but had relatively more of the former. With respect to substrate-profile the enzyme resembled the plasmid specified TEM-type beta-lactamases commonly encountered in Gram-negative bacteria. The enzyme was insensitive to p-chloromercuribenzoate, sodium chloride, or iodine inhibition. 相似文献
112.
113.
G Rodriguez-Tarduchy P Malde A Lopez-Rivas M K Collins 《Journal of immunology (Baltimore, Md. : 1950)》1992,148(5):1416-1422
Calcium ionophores inhibit apoptosis in the IL-3-dependent cell line BAF3 and maintain the cells in a viable noncycling state. In this report, an identical effect of ionophore was also demonstrated on the multipotent IL-3-dependent progenitor cell line FDCP-MIX and on the primary IL-3-dependent cell population that could be cultured from murine bone marrow. Inhibition of apoptosis required extracellular calcium and could be blocked by cyclosporin A. Nuclei from IL-3-dependent cells were found to lack a calcium-activatable nuclease that degrades chromatin in the linker region between nucleosomes, unlike the nuclei of lymphoid cells. The mechanism of action of calcium ionophore could be divided into two distinct steps. First, ionophore induced the production of a survival factor that stimulated DNA synthesis and was identified as IL-4. Second, ionophore inhibited the cell cycle of the various IL-3-dependent cells. IL-4 production could be inhibited by cyclosporin A and required extracellular calcium, whereas cell cycle arrest did not. This implied that factor production was the step that was necessary for inhibition of apoptosis and maintenance of cell viability. This was confirmed by the use of an anti-IL-4R antibody, which blocked the inhibition of apoptosis induced by calcium ionophores. 相似文献
114.
Victoria V. Hargreaves Scarlet S. Shell Dan J. Mazur Martin T. Hess Richard D. Kolodner 《The Journal of biological chemistry》2010,285(12):9301-9310
Indirect evidence has suggested that the Msh2-Msh6 mispair-binding complex undergoes conformational changes upon binding of ATP and mispairs, resulting in the formation of Msh2-Msh6 sliding clamps and licensing the formation of Msh2-Msh6-Mlh1-Pms1 ternary complexes. Here, we have studied eight mutant Msh2-Msh6 complexes with defective responses to nucleotide binding and/or mispair binding and used them to study the conformational changes required for sliding clamp formation and ternary complex assembly. ATP binding to the Msh6 nucleotide-binding site results in a conformational change that allows binding of ATP to the Msh2 nucleotide-binding site, although ATP binding to the two nucleotide-binding sites appears to be uncoupled in some mutant complexes. The formation of Msh2-Msh6-Mlh1-Pms1 ternary complexes requires ATP binding to only the Msh6 nucleotide-binding site, whereas the formation of Msh2-Msh6 sliding clamps requires ATP binding to both the Msh2 and Msh6 nucleotide-binding sites. In addition, the properties of the different mutant complexes suggest that distinct conformational states mediated by communication between the Msh2 and Msh6 nucleotide-binding sites are required for the formation of ternary complexes and sliding clamps. 相似文献
115.
M V Sauer 《The Western journal of medicine》1994,161(2):164-165
116.
117.
118.
119.
—A resolution of the enhancement of protein synthesis in the visual cortex of rats during first exposure to light (Richardson and Rose , 1972) was achieved by polyacrylamide gel electrophoresis using a double-labelling technique. Differential incorporation of lysine was established between exposed and control animals in two fractions of the soluble proteins and seven fractions of the insoluble proteins. This suggests that exposure to a new experience of this type involves a specific effect on protein synthesis, rather than a general stimulation across all fractions. 相似文献
120.