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131.
Carotenoids present in lipids extracted from the cyanobacterium Synechococcus 6716 indicate trans-membrane potential in proteoliposomes reconstituted from these lipids and the ATPase complex isolated from the same organism. A carotenoid absorbance band shift to a longer wavelength is obtained with valinomycin-induced potassium ion diffusion potentials, irrespective of the polarity of the potassium gradient. In contrast to this, the (externally added) probe oxonol VI only shows an absorbance band shift when the external potassium ion concentration is higher than the internal one. In liposomes without ATPase complex, no carotenoid absorbance band shifts were observed. 相似文献
132.
Amino acid sulfur as a source of sulfate for sulfated proteoglycans produced by Swiss mouse 3T3 cells 总被引:3,自引:0,他引:3
The uptake of sulfate by Swiss mouse 3T3 cells is blocked in the presence of 1 mM 4-isothiocyano-4'-acetamido-stilbene-2,2-disulfonic acid (SITS). In the absence of an exogenous source of sulfate, glycosaminoglycans produced by cells in the presence of the inhibitor are sulfated to the same extent as those produced by cells grown in its absence. The sulfate utilized in the absence of medium sulfate has been identified as that produced by the oxidation of the sulfur present in the amino acids cysteine and methionine. This finding indicates that, under conditions of restricted exogenous sulfate, caution is needed in the interpretation of data obtained with the use of [35S]methionine and/or [35S]cysteine as a general protein label, since both tyrosine and a variety of types of protein-linked carbohydrate chains may be modified by sulfation. 相似文献
133.
134.
Levels of 7.8, 18.5 and 26 mole % deuterium oxide were administered sequentially to (Japanese quail) via the drinking water. The primary effect observed was on egg frequency, which decreased from a normal level of 0.89 for 7.8 mole % D2O to a low of 0.38 during the administration of 26 mole % D2O. Adverse symptoms, such as hyperexcitability, convulsions, skin ulcerations, comatosity, weight loss, or death, which have been associated with deuterium toxicity in other animals, were not observed in these experiments. The amount of deuterium deposited in the water of the egg was 6.9, 13.98, and 19.83 mole % when 7.8, 18.5 and 26 mole % deuterium respectively was administered. For each period, the deuterium content of egg water rapidly reached a maximum concentration after which the concentration decreased slightly. This dilution effect has not been noted previously in body fluids from other animals. 相似文献
135.
The potential for and constraints on the evolution of compensatory ability in Asclepias syriaca 总被引:2,自引:0,他引:2
To investigate the potential for and constraints on the evolution of compensatory ability, we performed a greenhouse experiment
using Asclepias syriaca in which foliar damage and soil nutrient concentration were manipulated. Under low nutrient conditions, significant genetic
variation was detected for allocation patterns and for compensatory ability. Furthermore, resource allocation to storage was
positively, genetically correlated both with compensatory ability and biomass when damaged, the last two being positively,
genetically correlated with each other. Thus, in the low nutrient environment, compensatory ability via resource allocation
to storage provided greater biomass when damaged. A negative genetic correlation between compensatory ability and plant biomass
when undamaged suggests that this mechanism entailed an allocation cost, which would constrain the evolution of greater compensatory
ability when nutrients are limited. Under high nutrient conditions, neither compensatory ability nor allocation patterns predicted
biomass when damaged, even though genetic variation in compensatory ability existed. Instead, plant biomass when undamaged
predicted biomass when damaged. The differences in outcomes between the two nutrient treatments highlight the importance of
considering the possible range of environmental conditions that a genotype may experience. Furthermore, traits that conferred
compensatory ability did not necessarily contribute to biomass when damaged, demonstrating that it is critical to examine
both compensatory ability and biomass when damaged to determine whether selection by herbivores can favor the evolution of
increased compensation.
Received: 2 April 1999 / Accepted: 21 September 1999 相似文献
136.
Methods for inducing high-frequency somatic embryos in cassava on cotyledons and 33 clonal accessions by the addition of supplementary copper sulphate to the induction medium were investigated. The addition of copper sulphate enhanced primary embryo induction and significantly increased secondary embryo production. All accessions from Latin America (CIAT) were embryogenically competent on medium supplemented with 8 mg l-1 2,4-dichlorophenoxyacetic acid (2,4-D) plus 1 µM copper sulphate as were 15 of the 18 accessions from Africa. The percentage of calli producing somatic embryos ranged from 7.5% in M. Bra 12 to 100% in M. Col. 1505, while the number of embryos produced per callus ranged from 0.3 in M. Bra 383 to 13.5 in TEK. The frequency of embryo production was dependent on the concentration of copper sulphate. The number of primary embryos produced per callus was also comparatively higher in the medium supplemented with copper sulphate than in the controls. The optimal concentration of copper sulphate for number of embryos produced in most accessions was 5 µM, and at this concentration the number of embryos produced was double that of the controls. Copper sulphate also reduced the maturation time of somatic embryos to 25 days from embryo initiation. High levels of 2,4-D were detrimental to embryo production. Similarly, fragmented embryos incubated in the dark produced more embryos tan those incubated under light conditions. On the basis of these results, the use of cassava somatic embryo micropropagules for germplasm conservation and synthetic seed development seems to be a strong possibility. 相似文献
137.
Study of the posterior abdominal epidermis in hypophysectomized/thyroidectomized male and female tokays following surgery, and subsequent androgen therapy, indicates that, contrary to a previous model, all aspects of β-gland differentiation are under direct androgenic control. On the other hand, another epidermal specialization, the digital foot-pad, shows a pattern of histogenesis directly comparable to that of β-glands, but is unaffected by androgens. These data are discussed with respect to the evolution of glandular epidermal specializations in gekkonid lizards and the possible role of androgens in modifying the control of cell differentiation in lizard epidermis. 相似文献
138.
139.
The photoacoustic spectroscopic studies of purple pigmented leaves revealed the occurrence of anthocyanins and betalains in
some local weed species growing on soils with low moisture levels. The pigmentation intensities were higher in C4 plants than in C3 plants. An inverse correlation was observed between pigmentation intensities and soil moisture levels.
This work is a part of the UNEP Research Project granted to Prof. D. O. Hall, UNEP Coordinator, Department of Plant Sciences,
King’s College; London. 相似文献
140.
S Kumar M Antony N K Mehrotra 《Biochemical and biophysical research communications》1984,125(3):967-973
Single cutaneous application of 12-O-tetradecanoyl-phorbol-13-acetate (TPA) increased epidermal oxidised glutathione reductase activity in adult mouse by almost 100%. Pretreatment of animals with vitamin A for a week resulted in 75% inhibition of TPA induced change in the enzyme activity which remained unaffected in skin treated with vitamin A alone. This biochemical change in skin induced by TPA and modulated by vitamin A has been discussed in relation to epidermal hyperplasia. 相似文献