全文获取类型
收费全文 | 722804篇 |
免费 | 81040篇 |
国内免费 | 899篇 |
专业分类
804743篇 |
出版年
2018年 | 6492篇 |
2016年 | 8893篇 |
2015年 | 12638篇 |
2014年 | 14740篇 |
2013年 | 20274篇 |
2012年 | 23541篇 |
2011年 | 23869篇 |
2010年 | 16057篇 |
2009年 | 14581篇 |
2008年 | 21119篇 |
2007年 | 21884篇 |
2006年 | 20480篇 |
2005年 | 19588篇 |
2004年 | 19451篇 |
2003年 | 18429篇 |
2002年 | 18132篇 |
2001年 | 33319篇 |
2000年 | 33540篇 |
1999年 | 26445篇 |
1998年 | 9140篇 |
1997年 | 9307篇 |
1996年 | 8826篇 |
1995年 | 8379篇 |
1994年 | 8074篇 |
1993年 | 7921篇 |
1992年 | 21842篇 |
1991年 | 21536篇 |
1990年 | 20872篇 |
1989年 | 20109篇 |
1988年 | 18647篇 |
1987年 | 17470篇 |
1986年 | 16450篇 |
1985年 | 16186篇 |
1984年 | 13268篇 |
1983年 | 11562篇 |
1982年 | 8633篇 |
1981年 | 7684篇 |
1980年 | 7138篇 |
1979年 | 12578篇 |
1978年 | 9887篇 |
1977年 | 8832篇 |
1976年 | 8198篇 |
1975年 | 9477篇 |
1974年 | 10152篇 |
1973年 | 9925篇 |
1972年 | 8952篇 |
1971年 | 8113篇 |
1970年 | 7067篇 |
1969年 | 6810篇 |
1968年 | 6212篇 |
排序方式: 共有10000条查询结果,搜索用时 0 毫秒
981.
M. L. Rabinovich 《Applied Biochemistry and Microbiology》2006,42(1):1-26
Development of national research of cellulose-degrading microorganisms and enzymes is reviewed, with emphasis on the prospects of producing ethanol from cellulose materials using cellulolytic enzymes. Leading Russian research groups in this field are introduced. A section of the review analyzes problems and prospects of setting up environmentally friendly production of motor biofuels from renewable raw materials of plant origin (an approach developed in Russia). 相似文献
982.
Y Midorikawa H Hibasami P Gasaluck H Yoshimura A Masuji K Nakashima M Imai 《The Journal of applied bacteriology》1991,70(4):291-293
Metabolic and antiproliferative effects of methylglyoxal bis(butylamidinohydrazone) (MGBB) and methylglyoxal bis(cyclopentylamidinohydrazone) (MGBCP), inhibitors for polyamine biosynthetic pathway, on Escherichia coli, Shigella sonnei, Aeromonas sobria, Aeromonas hydrophila and Vibrio cholerae were investigated. MGBB at the concentration of 100 mumol/l depleted intracellular putrescine and spermidine concentrations of E. coli to 25 and 20% of the controls, respectively, while MGBCP depressed their concentrations to 38 and 24%, respectively. In these polyamine-depleted E. coli cells the syntheses of RNA, DNA and protein decreased to 13, 54 and 29% of the control, respectively, with MGBB and to 23, 71 and 55%, respectively, with MGBCP. The minimum inhibitory concentrations (MIC) of MGBB for the growth of A. sobria, E. coli, A. hydrophila, V. cholerae and Sh. sonnei were estimated to be 50, 160, 240, 285 and 320 mumol/l, respectively, whereas those of MGBCP were slightly higher for respective bacteria. 相似文献
983.
Recent reports suggest that prostaglandins, rather than cAMP, play a major role in mediating cholera toxin-induced water and electrolyte secretion from rabbit intestinal loops. We examined the role of prostaglandins in mediating toxin-induced pancreatic and gastric exocrine secretion. In these tissues, indomethacin, a potent inhibitor of prostaglandin synthesis, did not alter the stimulatory effects of cholera toxin on increases in cellular cAMP or enzyme secretion. Moreover, the addition of cholera toxin did not alter prostaglandin E2 release from either tissue. In contrast to their effects in rabbit intestinal loops, prostaglandins do not regulate cholera toxin-induced enzyme secretion from the guinea pig pancreas or stomach. 相似文献
984.
985.
986.
Abstract— Autolytic changes in the mouse brain, occurring during immersion of the animal in liquid nitrogen, were evaluated by measuring the tissue concentrations of glucose, lactate, pyruvate, α-oxoglutarate, phosphocreatine, creatine, ATP, ADP and AMP. The values thus obtained were compared with those obtained in paralysed mice under nitrous oxide anaesthesia, the brains of which were frozen in such a way that arterial blood pressure and oxygénation were upheld during the freezing. Immersion of unanaesthetized mice in liquid nitrogen gave rise to significant alterations in phosphocreatine, creatine, lactate, lactate/pyruvate ratio, ADP and AMP. A comparison with values obtained in paralysed and anaesthetized mice that were frozen by immersion in liquid nitrogen showed that the metabolic changes observed in the unanaesthetized animals could not be caused by an anaesthetic effect on the metabolic pattern. It is concluded that autolysis in the mouse brain occurs during immersion of the animal in a coolant, mainly because arterial hypoxia develops before the tissue is frozen. A comparison with previous results on rat cerebral cortex indicates that mice offer no advantage for studies of cerebral metabolites in unanaesthetized animals. In both species, accurate analyses of labile cerebral metabolites require that the brain is frozen in a way that prevents arterial hypoxia during the fixation of the tissue. 相似文献
987.
Biochemical and genetic evidence for a macromolecular -glucuronidase complex in microsomal membranes 总被引:10,自引:0,他引:10
In the mouse β-glucuronidase is present in both microsomes and lysosomes and the enzyme at both sites is coded by the same structural gene. Electrophoresis on polyacrylamide gels showed that liver, kidney and lung from normal strains contained five enzyme forms designated L, M1, M2, M3 and M4 in order of decreasing mobility toward the anode. Band L is found primarily in lysosomes and is a tetramer of 260,000 molecular weight. Bands M1 to M4 are found exclusively in microsomes and range in molecular weight from 310,000 to 470,000. The increase in molecular weight is due to sequential addition of an accessory protein chain. When glucuronidase is highly induced in kidneys of female mice by injection of dihydrotestosterone, a sixth electrophoretic form of glucuronidase, designated X, appears. Form X appears early in induction, is localized in microsomes, and has a molecular weight (260,000) equal to that of the tetramer form L.Mice homozygous for the eg ° mutation, and thus deficient in microsomal glucuronidase, completely lack the microsomal forms M1 to M4. They do contain form X, and this increases after testosterone induction in kidney. The form X present in eg ° mice is indistinguishable from the form X seen in normal induced kidney.It appears that mice synthesize two different tetrameric forms of glucuronidase from the same structural gene. One, form L, is lysosomal; the other, form X, gives rise to microsomal enzyme forms M1 to M4 by the successive addition of up to four accessory protein chains. The eg ° mutant is blocked in the conversion of X to M1. 相似文献
988.
989.
990.