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51.
A summary delineating the large scale synthetic studies to prepare labeled precursors of ribonucleosides-3',4',5',5'-2H4 and -2',3',4',5',5'-2H5 from D-glucose is presented. The recycling of deuterium-labeled by-products has been devised to give a high overall yield of the intermediates and an expedient protocol has been elaborated for the conversion of 3-O-benzyl-alpha,beta-D-allofuranose-3,4-d2 6 to 1-O-methyl-3-O-benzyl-2-O-t-butyldimethylsilyl-alpha,beta-D-ribofuranose-3,4,5,5'-d4 16 (precursor of ribonucleosides-3',4',5',5'-2H4) or to 1-O-methyl-3,5-di-O-benzyl-alpha,beta-D-ribofuranose-3,4,5,5'-d4 18 (precursor of ribonucleosides-3',4',5',5'-2H4). 相似文献
52.
In a screen for calcium-regulated gene expression during growth and development ofDictyostelium discoideum we have identified an asparaginyl tRNA synthetase (ddAsnRS) gene, the second tRNA synthetase gene identified in this organism. TheddAsnRS gene shows many unique features. One, it is repressed by lowering cellular calcium, making it the first known calcium-regulated
tRNA synthetase. Two, despite the calcium-dependence, its expression is unaltered during the cell cycle, making this the firstD. discoideum gene to show a calcium-dependent but cell cycle phase-independent expression. Finally, the N-terminal domain of the predicted
ddAsnRS protein shows higher sequence similarity to Glutaminyl tRNA synthetases than to other Asn tRNA synthetases. These
unique features of theAsnRS from this primitive eukaryote not only point to a novel mechanism regulating the components of translation machinery and
gene expression by calcium, but also hint at a link between the evolution ofGlnRS andAsnRS in eukaryotes. 相似文献
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54.
Jyoti Pande Magdalena M Szewczyk Iwona Kuszczak Shawn Grover E Escher Ashok K Grover 《Journal of cellular and molecular medicine》2008,12(3):1049-1060
Coronary artery smooth muscle expresses the plasma membrane Ca(2+) pump (PMCA) isoforms PMCA4 and PMCA1. We previously reported the peptide inhibitor caloxin 1b1 that was obtained by using extracellular domain 1 of PMCA4 as the target (Am J Physiol Cell.290 [2006] C1341). To engineer inhibitors with greater affinity and isoform selectivity, we have now created a phage display library of caloxin 1b1-like peptides. We screened this library by affinity chromatography with PMCA from erythrocyte ghosts that contain mainly PMCA4 to obtain caloxin 1c2. Key properties of caloxin 1c2 are (a) Ki = 2.3 +/- 0.3 microM which corresponds to a 20x higher affinity for PMCA4 than that of caloxin 1b1 and (b) it is selective for PMCA4 since it has greater than 10-fold affinity for PMCA4 than for PMCA1, 2 or 3. It had the following functional effects on coronary artery smooth muscle: (a) it increased basal tone of the de-endothelialized arteries; the increase being similar at 10, 20 or 50 microM, and (b) it enhanced the increase in the force of contraction at 0.05 but not at 1.6 mM extracellular Ca(2+) when Ca(2+) extrusion via the Na(+)-Ca(2+) exchanger and the sarco/endoplasmic reticulum Ca(2+) pump were inhibited. We conclude that PMCA4 is pivotal to Ca(2+) extrusion in coronary artery smooth muscle. We anticipate caloxin 1c2 to aid in understanding the role of PMCA4 in signal transduction and home-ostasis due to its isoform selectivity and ability to act when added extracellularly. 相似文献
55.
56.
Mechanical activity of cells and the stress imposed on them by extracellular environment is a constant source of injury to the plasma membrane (PM). In invasive tumor cells, increased motility together with the harsh environment of the tumor stroma further increases the risk of PM injury. The impact of these stresses on tumor cell plasma membrane and mechanism by which tumor cells repair the PM damage are poorly understood. Ca2+ entry through the injured PM initiates repair of the PM. Depending on the cell type, different organelles and proteins respond to this Ca2+ entry and facilitate repair of the damaged plasma membrane. We recently identified that proteins expressed in various metastatic cancers including Ca2+-binding EF hand protein S100A11 and its binding partner annexin A2 are used by tumor cells for plasma membrane repair (PMR). Here we will discuss the involvement of S100, annexin proteins and their regulation of actin cytoskeleton, leading to PMR. Additionally, we will show that another S100 member – S100A4 accumulates at the injured PM. These findings reveal a new role for the S100 and annexin protein up regulation in metastatic cancers and identify these proteins and PMR as targets for treating metastatic cancers. 相似文献
57.
58.
Nagesh K. Tripathi Jyoti Shukla Karttik C. Biswal P. V. Lakshmana Rao 《Applied microbiology and biotechnology》2010,86(6):1795-1803
Japanese encephalitis (JE) is one of the leading causes of acute encephalopathy affecting children and adolescents in the
tropics. Optimization of media was carried out for enhanced production of recombinant JE virus envelope domain III (EDIII)
protein in Escherichia coli. Furthermore, batch and fed-batch cultivation process in E. coli was also developed in optimized medium. Expression of this protein in E. coli was induced with 1 mM isopropyl-β-thiogalactoside and yielded an insoluble protein aggregating to form inclusion bodies.
The inclusion bodies were solubilized in 8 M urea, and the protein was purified under denaturing conditions using Ni-NTA affinity
chromatography. After fed-batch cultivation, the recombinant E. coli resulted in cell dry weight and purified protein about 36.45 g l−1 and 720 mg l−1 of culture, respectively. The purity of the recombinant JE virus EDIII protein was checked by sodium dodecyl sulfate polyacrylamide
gel electrophoresis analysis, and reactivity of this protein was determined by Western blotting and ELISA with JE virus-infected
human serum samples. These results establish the application of this protein to be used for the diagnosis of JE virus infection
or for further studies in vaccine development. This process may also be suitable for the high-yield production of other recombinant
viral proteins. 相似文献
59.
Shashi Sharma Paban Kumar Dash S. R. Santhosh Jyoti Shukla Manmohan Parida P. V. Lakshmana Rao 《Molecular biotechnology》2010,45(1):49-55
Chikungunya is one of the most important emerging arboviral infections of public health significance. Due to lack of a licensed
vaccine, rapid diagnosis plays an important role in early management of patients. In this study, a QC-RT–PCR assay was developed
to quantify Chikungunya virus (CHIKV) RNA by targeting the conserved region of E1 gene. A competitor molecule containing an
internal insertion was generated, which provided a stringent control of the quantification process. The introduction of 10-fold
serially diluted competitor in each reaction was further used to determine sensitivity. The applicability of this assay for
quantification of CHIKV RNA was evaluated with human clinical samples, and the results were compared with real-time quantitative
RT–PCR. The sensitivity of this assay was estimated to be 100 RNA copies per reaction with a dynamic detection range of 102 to 1010 copies. Specificity was confirmed using closely related alpha and flaviviruses. The comparison of QC-RT–PCR result with real-time
RT–PCR revealed 100% concordance for the detection of CHIKV in clinical samples. These findings demonstrated that the reported
assay is convenient, sensitive and accurate method and has the potential usefulness for clinical diagnosis due to simultaneous
detection and quantification of CHIKV in acute-phase serum samples. 相似文献
60.
Jyoti P. Jadhav Swapnil S. Phugare Rhishikesh S. Dhanve Shekhar B. Jadhav 《Biodegradation》2010,21(3):453-463
A newly isolated novel bacterium from sediments contaminated with dyestuff was identified as Pseudomonas aeruginosa strain BCH by 16S rRNA gene sequence analysis. The bacterium was extraordinarily active and operative over a wide rage of
temperature (10–60°C) and salinity (5–6%), for decolorization of Direct Orange 39 (Orange TGLL) at optimum pH 7. This strain
was capable of decolorizing Direct Orange 39; 50 mg l−1 within 45 ± 5 min, with 93.06% decolorization, while maximally it could decolorize 1.5 g l−1 of dye within 48 h with 60% decolorization. Analytical studies as, UV–Vis spectroscopy, FTIR, HPLC were employed to confirm
the biodegradation of dye and formation of new metabolites. Induction in the activities of lignin peroxidases, DCIP reductase
as well as tyrosinase was observed, indicating the significant role of these enzymes in biodegradation of Direct Orange 39.
Toxicity studies with Phaseolus mungo and Triticum aestivum revealed the non-toxic nature of degraded metabolites. 相似文献