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排序方式: 共有456条查询结果,搜索用时 5 毫秒
81.
Shaham Beg Rohan Bareja Kentaro Ohara Kenneth Wha Eng David C. Wilkes David J. Pisapia Wael Al Zoughbi Sarah Kudman Wei Zhang Rema Rao Jyothi Manohar Troy Kane Michael Sigouros Jenny Zhaoying Xiang Francesca Khani Brian D. Robinson Bishoy M. Faltas Cora N. Sternberg Andrea Sboner Himisha Beltran Olivier Elemento Juan Miguel Mosquera 《Translational oncology》2021,14(1)
82.
Jyothi C. Talakad P. Ross Wilderman Dmitri R. Davydov 《Archives of biochemistry and biophysics》2010,494(2):151-158
Rational mutagenesis was used to improve the thermal stability of human cytochrome P450 2B6 and canine P450 2B11. Comparison of the amino acid sequences revealed seven sites that are conserved between the stable 2B1 and 2B4 but different from those found in the less stable 2B6 and 2B11. P334S was the only mutant that showed increased heterologous expression levels and thermal stability in both 2B6 and 2B11. The mechanism of this effect was explored with pressure-perturbation spectroscopy. Compressibility of the heme pocket in variants of all four CYP2B enzymes containing proline at position 334 are characterized by lower compressibility than their more stable serine 334 counterpart. Therefore, the stabilizing effect of P334S is associated with increased conformational flexibility in the region of the heme pocket. Improved stability of P334S 2B6 and 2B11 may facilitate the studies of these enzymes by X-ray crystallography and biophysical techniques. 相似文献
83.
Sandhya Manohar Qing Yu Steven P. Gygi Randall W. King 《Molecular & cellular proteomics : MCP》2020,19(9):1450-1467
Highlights
- •Chemical proteomics in G1 cells treated with small molecule APC/C inhibitors reveals novel putative APC/C substrates.
- •The insulin receptor adaptor IRS2 is an APC/C substrate that is targeted for degradation by APC/CCdh1.
- •Quantitative proteomics in IRS2 knockout cells reveals a deficiency in cell cycle related protein expression.
- •IRS2 promotes a robust spindle assembly checkpoint (SAC) during M-phase.
84.
85.
Ghosh Anupam Dhar Dwivedi Shyam Murli Manohar Ghadi Hemant Chinnamuthu Paulsamy Chakrabarti Subhananda Mondal Aniruddha 《Plasmonics (Norwell, Mass.)》2018,13(3):1105-1113
Plasmonics - Ag nanoparticles (NPs) were deposited on the sol-gel-processed Erbium-doped Indium Oxide (In2O3:Er) thin films (TFs) using thermal evaporation cum glancing angle deposition technique... 相似文献
86.
Sandip K. Wagh Praful P. Gadge Manohar V. Padul 《Probiotics and antimicrobial proteins》2018,10(4):662-667
Peptidase therapy is suggested to be effective to minimize gliadin toxicity in celiac disease (CD). Hence, present study deals with gliadin-hydrolysing peptidases. The efficient peptidase from the Bacillus tequilensis was purified using ammonium sulfate fractionation and preparative electrophoresis. Analysis of in-solution and in-gel hydrolysis of gliadin using one and two-dimensional SDS-PAGE revealed nearly complete hydrolysis of gliadin peptides after 180 min of incubation with B. tequilensis protease. Purified peptidase was found to be stable at acidic (pH 3.5) to neutral (pH 7.2) pH range. The molecular mass and isoelectric point of the peptidase were observed around 29 kDa and 5.2, respectively. The internal protein sequence obtained through mass spectrometric analysis suggested that peptidase might belong to peptidase S9 family known for prolyl-specific peptidases. This study recommends the possible applicability of this peptidase for elimination of immunotoxic gliadin peptides and may prove useful in CD treatment. 相似文献
87.
Rabiya Bi H. C. Lohithaswa S. Lokesh K. R. Sunil Kumar H. B. Shilpa K. Jyothi K. Vinutha Shailaja Hittalmani 《Journal of genetics》2018,97(1):117-138
The expressed sequence tags (ESTs) of common bean were BLAST aligned with barred medic genome sequence and developed 1196 conserved intron spanning primers (CISPs) to facilitate genetic studies in legumes. Randomly selected 288 CISPs, representing loci on barrel medic genome, were tested on 10 selected members of legume family. On the source taxa, the highest single copy amplification success rates of 61.8% (barrel medic) and 56.2% (common bean) was obtained. The success rate of markers was 54.5% in cowpea followed by 53.5% in pigeonpea and chickpea, signifying cross taxon amplification and their potential use in comparative genomics. However, relatively low percentages of primer set amplified (40–43%) in soybean, urdbean and peanut. Further, these primers were tested on different varieties of chickpea, pigeonpea and cowpea. The PCR products were sequenced and aligned which resulted in detection of 26 SNPs and eight INDeLs in cowpea, seven SNPs and two INDeLs in chickpea and 27 SNPs and 14 INDeLs in pigeonpea. These SNPs were successfully converted in to size variation for gel-based genotyping. The CISP markers developed in this study are expected to aid in map saturation of legumes and in marker-assisted selection for accelerated crop improvement. 相似文献
88.
Kevin Tran Chandrasekhar Gurramkonda Merideth A. Cooper Manohar Pilli Joseph E. Taris Nicholas Selock Tzu‐Chiang Han Michael Tolosa Adil Zuber Chariz Peñalber‐Johnstone Christina Dinkins Niloufar Pezeshk Yordan Kostov Douglas D. Frey Leah Tolosa David W. Wood Govind Rao 《Biotechnology and bioengineering》2018,115(1):92-102
The use of cell‐free systems to produce recombinant proteins has grown rapidly over the past decade. In particular, cell‐free protein synthesis (CFPS) systems based on mammalian cells provide alternative methods for the production of many proteins, including those that contain disulfide bonds, glycosylation, and complex structures such as monoclonal antibodies. In the present study, we show robust production of turbo green fluorescent protein (tGFP) and streptokinase in a cell‐free system using instrumented mini‐bioreactors for highly reproducible protein production. We achieved recombinant protein production (~600 μg/ml of tGFP and 500 μg/ml streptokinase) in 2.5 hr of expression time, comparable to previously reported yields for cell‐free protein expression. Also, we demonstrate the use of two different affinity tags for product capture and compare those to a tag‐free self‐cleaving intein capture technology. The intein purification method provided a product recovery of 86%, compared with 52% for conventionally tagged proteins, while resulting in a 30% increase in total units of activity of purified recombinant streptokinase compared with conventionally tagged proteins. These promising beneficial features combined with the intein technology makes feasible the development of dose‐level production of therapeutic proteins at the point‐of‐care. 相似文献
89.
Improving the recombinant human erythropoietin glycosylation using microsome supplementation in CHO cell‐free system 下载免费PDF全文
90.