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561.
MD ABDUR RAHIM HOONHEE SEO SUKYUNG KIM YOON KYOUNG JEONG HANIEH TAJDOZIAN MIJUNG KIM SAEBIM LEE HO-YEON SONG 《Polish journal of microbiology》2021,70(1):117
Staphylococcus aureus is currently a significant multidrug-resistant bacterium, causing severe healthcare-associated and community-acquired infections worldwide. The current antibiotic regimen against this pathogen is becoming ineffective due to resistance, in addition, they disrupt the normal microbiota. It highlights the urgent need for a pathogen-specific drug with high antibacterial efficacy against S. aureus. α-Viniferin, a bioactive phytochemical compound, has been reported to have excellent anti-Staphylococcus efficacy as a topical agent. However, so far, there were no clinical trials that have been conducted to elucidate its efficacy. The present study aimed to investigate the antibacterial efficacy of α-viniferin against S. aureus in a ten-day clinical trial. Based on the results, α-viniferin showed 50% minimum inhibitory concentrations (MIC50 values) of 7.8 μg/ml in culture broth medium. α-Viniferin was administered in the nares three times a day for ten days using a sterile cotton swab stick. Nasal swab specimens were collected before (0 days) and after finishing the trial (10th day), and then analyzed. In the culture and RT-PCR-based analysis, S. ureus was reduced significantly: 0.01. In addition, 16S ribosomal RNA-based amplicon sequencing analysis showed that S. aureus reduced from 51.03% to 23.99% at the genus level. RNA-seq analysis was also done to gain insights into molecular mechanisms of α-viniferin against S. aureus, which revealed that some gene groups were reduced in 5-fold FC cutoff at two times MIC conditions. The study results demonstrate α-viniferin as a potential S. aureus-specific drug candidate. 相似文献
562.
Phinitphong Sarichai MSc Songphon Buddhasiri DVM Georgia E. Walters BSc Banyong Khantawa MSc Thattawan Kaewsakhorn DVM PhD Kanittha Chantarasakha BSc Surapun Tepaamorndech PhD Parameth Thiennimitr MD PhD 《Microbiology and immunology》2020,64(10):679-693
Salmonella enterica serovar Typhimurium (S. Typhimurium [STM]) is a leading cause of nontyphoidal salmonellosis (NTS) worldwide. The pathogenesis of NTS has been studied extensively using a streptomycin-pretreated mouse colitis model with the limited numbers of laboratory STM strains. However, the pathogenicity of the clinically isolated STM (STMC) strains endemic in Thailand in mice has not been explored. The aim of this study was to compare the pathogenicity of STMC strains collected from Northern Thailand with the laboratory STM (IR715) in mice. Five STMC isolates were obtained from the stool cultures of patients with acute NTS admitted to Maharaj Nakorn Chiang Mai Hospital in 2016 and 2017. Detection of virulence genes and sequence type (ST) of the strains was performed. Female C57BL/6 mice were pretreated with streptomycin sulfate 1 day prior to oral infection with STM. On Day 4 postinfection, mice were euthanized, and tissues were collected to analyze the bacterial numbers, tissue inflammation, and cecal histopathological score. We found that all five STMC strains are ST34 and conferred the same or reduced pathogenicity compared with that of IR715 in mice. A strain-specific effect of ST34 on mouse gut colonization was also observed. Thailand STM ST34 exhibited a significant attenuated systemic infection in mice possibly due to the lack of spvABC-containing virulence plasmid. 相似文献
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Dandy–Walker malformation (DWM) is a rare intracranial congenital abnormality that affects the cerebellum and some of its components; particularly cerebellar vermis, fourth ventricle and is characterized by an enlarged posterior fossa. Although there is an extensive list of signs attributed to DWM, final diagnosis is solely dependent on imaging techniques as there are no signs that are characteristic of DWM. This article reports a case with DWM who was diagnosed by magnetic resonance imaging. 相似文献
567.
A comparative study of the metabolism of 1,2,3 (14)C-ODAP and 4,5 (14)C-ODAP in mice, rats and chicks has been carried out. Following oral administration of 1,2,3 (14)C-ODAP to either black or white mice, nearly 16% of the radioactivity appeared in the expired CO2 within 8 h, while in the rat only 3% of it appeared and in chicks it was less than 2%. No 14CO2 appeared in the expired air in mice given 4,5 (14)C-ODAP. Electrophoregrams of the spot urine samples from the animals given 1,2,3 (14)C-ODAP showed the presence of one radioactive metabolite (metabolite-1) in addition to ODAP. While the urine from rats and mice given 4,5 (14)C-ODAP indicated the presence of metabolite-1 as well as 14C-oxalate, in chicks, however, no 14C-oxalate was present and only metabolite-1 could be detected. The results indicate that ODAP can to some extent undergo oxidation in vivo in mice (and to a lesser extent in rats) leading to the formation of CO2 and oxalate and a similar pathway might be more prominent in humans leading to a near complete oxidation of ODAP. 相似文献