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151.
Marija Sumakovic Jan Hegermann Ling Luo Steven J. Husson Katrin Schwarze Christian Olendrowitz Liliane Schoofs Janet Richmond Stefan Eimer 《The Journal of cell biology》2009,186(6):897-914
Small guanosine triphosphatases of the Rab family regulate intracellular vesicular trafficking. Rab2 is highly expressed in the nervous system, yet its function in neurons is unknown. In Caenorhabditis elegans, unc-108/rab-2 mutants have been isolated based on their locomotory defects. We show that the locomotion defects of rab-2 mutants are not caused by defects in synaptic vesicle release but by defects in dense core vesicle (DCV) signaling. DCVs in rab-2 mutants are often enlarged and heterogeneous in size; however, their number and distribution are not affected. This implicates Rab2 in the biogenesis of DCVs at the Golgi complex. We demonstrate that Rab2 is required to prevent DCV cargo from inappropriately entering late endosomal compartments during DCV maturation. Finally, we show that RIC-19, the C. elegans orthologue of the human diabetes autoantigen ICA69, is also involved in DCV maturation and is recruited to Golgi membranes by activated RAB-2. Thus, we propose that RAB-2 and its effector RIC-19 are required for neuronal DCV maturation. 相似文献
152.
153.
Mirna Patricia Santiago-Gmez Hoa Tran Thanh Joëlle De Coninck Rmy Cachon Slim Kermasha Jean-Marc Belin Patrick Gervais Florence Husson 《Process Biochemistry》2009,44(9):1013-1018
Hexanal produced by cells of a recombinant Yarrowia lipolytica yeast expressing the hydroperoxide lyase (HPL) from green bell pepper fruit was studied under oxido-reducing conditions using the reducing dithiotreitol and oxidizing potassium ferricyanide compounds. The combined effect of pH, linoleic acid 13-hydroperoxides concentration, temperature and oxido-reducing molecules on the hexanal production was studied. Significant positive effects for the hexanal production were found using high concentrations of hydroperoxides (100 mM, 30 g/L). Adding reducing molecules enhanced significantly hexanal production while the oxidizing molecules had an inhibitory effect. Combined effects of 13-hydroperoxides and dithiotreitol were optimised by a central composite design and a model was proposed. Finally, 6 mM (600 mg/L) of hexanal was obtained when 119 mM of 13-hydroperoxides (37 g/L) and 50 mM of dithiotreitol were introduced directly in the biocatalytic medium of the yeast Y. lipolytica. 相似文献
154.
Isabelle Chardon-Loriaux Maurice-Marie Debray Henri-Philippe Husson 《Phytochemistry》1978,17(9):1605-1608
Two monomeric and three dimeric new indole alkaloids derived from cleavamine have been isolated from the leaves and stem barks of Capuronetta elegans. Structural determination was made by physical and chemical correlations with the known major alkaloids of the plants. 相似文献
155.
Discovery and characterization of a conserved pigment dispersing factor-like neuropeptide pathway in Caenorhabditis elegans 总被引:1,自引:0,他引:1
156.
Joshua Osuofa Daniel Henn Jinxiang Zhou Anna Forsyth Scott M. Husson 《Biotechnology progress》2021,37(3):e3129
This contribution reports on a study using Purexa™-MQ multimodal anion-exchange (AEX) membranes for protein polishing at elevated solution conductivities. Dynamic binding capacities (DBC10) of bovine serum albumin (BSA), human immunoglobulins, and salmon sperm DNA (ss-DNA) are reported for various salt types, salt concentrations, flowrates, and pH. Using 1 mg/ml BSA, DBC10 values for Purexa™-MQ were >90 mg/ml at conductivities up to 15 mS/cm. The membranes maintained a high, salt-tolerant BSA DBC10 of 89.8 ± 2.7 (SD) over the course of 100 bind-elute cycles. Polishing studies with acidic and basic monoclonal antibodies at >2 kg/L loads showed that Purexa™-MQ had higher clearance of host cell proteins and aggregate species at high conductivity (13 mS/cm) and in the presence of phosphate than other commercial AEX media. Purexa™-MQ also had a high ss-DNA DBC10 of 50 mg/ml at conductivities up to 15 mS/cm, markedly outperforming other commercial products. In addition to the effectiveness of Purexa™-MQ for protein polishing at elevated solution conductivities, its unusually high binding capacity for ss-DNA indicates potential applications for plasmid DNA purification. 相似文献
157.
Annie Husson Mimouna Bouazza Catherine Buquet Rene Vaillant 《In vitro cellular & developmental biology. Plant》1984,20(4):314-320
Summary Fetal rat hepatocytes were isolated and cultured in primary culture to investigate activity changes of arginase under defined
conditions. In hormone-free medium, cultured cells maintained the enzyme activity at levels equal to that of freshly isolated
cells for at least 4 d. Arginase activity could be induced by dexamethasone in hepatocytes isolated from 16.5-d-old fetuses
although cells were competent to respond to glucagon only at the stage of 18.5 d. The combination of the two hormones induced
greater levels of arginase activity than the individual compounds. These findings indicate that glucocorticoid and glucagon
receptors appear early and sequentially before birth and reveal that cultured fetal hepatocytes provide a suitable system
for the investigation of the role of hormones in the initiation of enzyme synthesis.
This work was supported by the Institut National Scientifique et de la Recherche Médicale through Grant 85.80.117. 相似文献
158.
Marya Aziz Richard St-Louis Florence Husson 《Bioscience, biotechnology, and biochemistry》2016,80(11):2184-2191
The presence of selected dehydrogenases, including alcohol dehydrogenase (ADH-YL) and aldehyde dehydrogenase (ALDH-YL), in Yarrowia lipolytica JMY 861, and their potential role in flavor synthesis were investigated. The experimental findings showed that using reduced form of nicotinamide adenine dinucleotide (NADH) as cofactor, the ADH-YL activity in vitro was 6-fold higher than that with reduced form of nicotinamide adenine dinucleotide phosphate (NADPH); however, under the experimental conditions used in this study, an ALDH-YL activity was not detected. The in situ hexanal reduction reaction was found to be instantaneous; however, when the yeast cells suspension was diluted 150 times, the initial relative hexanal concentration was increased by 84.1%. The chromatographic analyses indicated the conversion, in situ, of linoleic acid hydroperoxides (HPODs) into volatile C6-compounds after 60 min of HPODs addition to the yeast cells suspension. 相似文献
159.
160.
Influence of various antibiotics on superoxide generation by normal human neutrophils 总被引:1,自引:0,他引:1
P Van der Auwera G Petrikkos M Husson J Klastersky 《Archives internationales de physiologie et de biochimie》1986,94(5):S23-S28
Among the several killing mechanisms displayed by human neutrophils, the oxidative system is the most efficient. We have studied the influence of various antibiotics on the generation of superoxide by isolated human polymorphonuclear leukocytes (PMNL) stimulated by phorbol-myristate acetate. Among the antibiotics tested, only coumermycin significantly inhibited superoxide generation; this effect was dose-related, it depended on extracellular calcium concentration and was potentiated by sub-inhibitory concentrations of calcium channel-blocking agents. Coumermycin inhibited the influx of calcium produced by the ionophore A23187 as well as directed chemotaxis in agar and the intracellular killing of a highly susceptible strain of S. aureus. These inhibitory effects required at least 15 min of preincubation of the PMNL. Coumermycin, at clinically achievable serum concentrations, significantly impaired several PMNL functions. The mechanism could be a specific or a non-specific interaction with calcium-channels. 相似文献