Experimental maduromycosis in the laboratory mouse

Summary 1. A 0.5 ml inoculum containing 1.4×10⁴ spores and/or mycelial fragments ofAllescheria boydii when injected intraperitoneally, did not cause death in 18–20 g female Swiss mice in 18 days.2. Focal, pin-point lesions developed in the liver and spleen of mice injected with the stock suspension and the 1:10 dilution of it (2.8×10⁴ and 2.8×10³ infective units ofA. boydii per ml, respectively); no lesions developed from the 1:100 dilution of the original suspension.3.Allescheria boydii was recovered from the livers and spleens of all mice, as well as from the peritoneal exudate of one mouse injected with the stock suspension ofA. boydii; the fungus was not isolated from heart's blood.4. Inability to cause death by the intraperitoneal injection ofA. boydii inocula might be attributed to the natural defense mechanisms in the mice or to the excessive dilution of the inoculum; it is improbable that any genetically controlled resistance to allescheriosis exists in the mice used in this study.Paper no. 664, Department of Botany and Plant Pathology, Ohio State University, 1735 Neil Avenue, Columbus 10, Ohio.This is the report of research done by the junior authors with the guidance of the senior author during a course in medical mycology in this department.     

Is the first rib a reliable indicator of age at death assessment? Test of the method developed by Kunos et al (1999)

The aim of this study was to test the method Kunos and his colleagues (1999) developed on the first rib to assess adult age-at-death. The method was applied on a sample of known age and sex, selected from a Thai collection. The procedure being subjective, we chose to estimate an age category rather than a precise age.

The results show that only 55% of the individuals are correctly classified and that the number of subjects over 60 years old is under-estimated. Three explanations are given: the modifications of the first rib related with ageing are more variable than previously stated, the technique is too subjective and difficult to apply and the morphological changes of the first rib are different between North-American and Thai people.     

Shear wave induced resonance elastography of soft heterogeneous media

In the context of ultrasound dynamic elastography imaging and characterization of venous thrombosis, we propose a method to induce mechanical resonance of confined soft heterogeneities embedded in homogenous media. Resonances are produced by the interaction of horizontally polarized shear (SH) waves with the mechanical heterogeneity. Due to such resonance phenomenon, which amplifies displacements up to 10 times compared to non-resonant condition, displacement images of the underlying structures are greatly contrasted allowing direct segmentation of the heterogeneity and a more precise measurement of displacements since the signal-to-noise ratio is enhanced. Coupled to an analytical model of wave scattering, the feasibility of shear wave induced resonance (SWIR) elastography to characterize the viscoelasticity of a mimicked venous thrombosis is demonstrated (with a maximum variability of 3% and 11% for elasticity and viscosity, respectively). More generally, the proposed method has the potential to characterize the viscoelastic properties of a variety of soft biological and industrial materials.     

Loads distributed in vivo among vertebrae,muscles, spinal ligaments,and intervertebral discs in a passively flexed lumbar spine

Biomechanics and Modeling in Mechanobiology - The load distribution among lumbar spinal structures—still an unanswered question—has been in the focus of this hybrid experimental and...     

Life cycle inventory dataset review criteria—a new proposal

Purpose

A review of LCA process datasets is an important element of quality assurance for databases and for other systems to provide LCA datasets. Somewhat surprisingly, a broadly accepted and applicable set of criteria for a review of LCA process datasets was lacking so far. Different LCA databases and frameworks are proposing and using different criteria for reviewing datasets. To close this gap, a set of criteria for reviewing LCA dataset has been developed within the Life Cycle Initiative.

Methods

Previous contributions to LCA dataset review have been analysed for a start, from ISO and various LCA databases. To avoid somewhat arbitrary review criteria, four basic rules are proposed which are to be fulfilled by any dataset. Further, concepts for assessing representativeness and relevance are introduced into the criteria set from established practices in statistics and materiality. To better structure the criteria and to ease their application, they are grouped into clusters. A first version of the developed review criteria was presented in two workshops with database providers and users on different levels of experience, and draft versions of the criteria were shared within the initiative. The current version of the criteria reflects feedback received from various stakeholders and has been applied and tested in a review for newly developed datasets in Brazil, Malaysia and Thailand.

Results and discussion

Overall, 14 criteria are proposed, which are organised in clusters. The clusters are goal, model, value, relevance and procedure. For several criteria, a more science-based definition and evaluation is proposed in comparison to ‘traditional’ LCA. While most of the criteria depend on the goal and scope of dataset development, a core set of criteria are seen as essential and independent from specific LCA modelling. For all the criteria, value scales are developed, typically using an ordinal scale, following the pedigree approach.

Conclusions

Review criteria for LCI datasets are now defined based on a stringent approach. They aim to be globally acceptable, considering also database interoperability and database management aspects, as well as feedback received from various stakeholders, and thus close an important gap in LCA dataset quality assurance. The criteria take many elements of already existing criteria but are the first to fully reflect the implications of the ISO data quality definition, and add new concepts for representativeness and relevance with the idea to better reflect scientific practice outside of the LCA domain. A first application in a review showed to be feasible, with a level of effort similar to applying other review criteria. Aspects not addressed yet are the review procedure and the mutual recognition of dataset reviews, and their application for a very high number of datasets.

     

Effects of corallivory and coral colony density on coral growth and survival

A suite of processes drive variation in coral populations in space and time, yet our understanding of how variation in coral density affects coral performance is limited. Theory predicts that reductions in density can send coral populations into a predator pit, where concentrated corallivory maintains corals at low densities. In reality, how variation in coral density alters corallivory rates is poorly resolved. Here, we experimentally quantified the effects of corallivory and coral density on growth and survival of small colonies of the staghorn coral Acropora pulchra. Our findings suggest that coral density and corallivory have strong but independent effects on coral performance. In the presence of corallivores, corals suffered high but density-independent mortality. When corallivores were excluded, however, vertical extension rates of colonies increased with increasing densities. While we found no evidence for a predator pit, our results suggest that spatio-temporal variation in corallivore and coral densities can fundamentally alter population dynamics via strong effects on juvenile corals.