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161.
162.
Agnes Schulze-Specking Justus Duyster Peter J. Gebicke-Haerter Siegfried Wurster Peter Dieter 《Cellular signalling》1991,3(6)
Fluoride elicited in liver macrophages a release of arachidonic acid and prostaglandins but not formation of inositol phosphates or superoxide. The effects of fluoride required extracellular calcium and were inhibited by staurosporine and by phorbol ester treatment of the cells. Furthermore, fluoride led to a translocation of protein kinase C from the cytosol to membranes. This indicates that the calcium-dependent protein kinase C is involved in the action of fluoride. Cholera toxin decreased the zymosan-induced release of arachidonic acid and prostaglandins but not of inositol phosphates or superoxide. Pertussis toxin ADP-ribosylated a 41,000 molecular weight membrane protein; enhanced specifically the zymosan-induced formation of prostaglandin(PG)E2 but did not affect the zymosan-induced release of arachidonic acid, PGD2, inositol phosphates or superoxide. These data suggest that activation of phospholipase (PL)A2, phosphoinositide (PI)-specific PLC and NADPH oxidase in liver macrophages is most probably not mediated by activation of guanine nucleotide binding (G)-proteins coupled directly to these enzymes. 相似文献
163.
Martin Diekmann Christian Andres Thomas Becker Jonathan Bennie Volker Blüml James M. Bullock Heike Culmsee Miriam Fanigliulo Annett Hahn Thilo Heinken Christoph Leuschner Stefanie Luka Justus Meißner Josef Müller Adrian Newton Cord Peppler‐Lisbach Gert Rosenthal Leon J. L. van den Berg Philippine Vergeer Karsten Wesche 《植被学杂志》2019,30(2):187-202
164.
165.
Birgit Wolters Kristin Hauschild Khald Blau Ines Mulder Benjamin Justus Heyde Søren J. Sørensen Jan Siemens Sven Jechalke Kornelia Smalla Joseph Nesme 《Environmental microbiology》2022,24(3):1573-1589
Soil fertilization with wastewater treatment plant (WWTP) biosolids is associated with the introduction of resistance genes (RGs), mobile genetic elements (MGEs) and potentially selective pollutants (antibiotics, heavy metals, disinfectants) into soil. Not much data are available on the parallel analysis of biosolid pollutant contents, RG/MGE abundances and microbial community composition. In the present study, DNA extracted from biosolids taken at 12 WWTPs (two large-scale, six middle-scale and four small-scale plants) was used to determine the abundance of RGs and MGEs via quantitative real-time PCR and the bacterial and archaeal community composition was assessed by 16S rRNA gene amplicon sequencing. Concentrations of heavy metals, antibiotics, the biocides triclosan, triclocarban and quaternary ammonium compounds (QACs) were measured. Strong and significant correlations were revealed between several target genes and concentrations of Cu, Zn, triclosan, several antibiotics and QACs. Interestingly, the size of the sewage treatment plant (inhabitant equivalents) was negatively correlated with antibiotic concentrations, RGs and MGEs abundances and had little influence on the load of metals and QACs or the microbial community composition. Biosolids from WWTPs with anaerobic treatment and hospitals in their catchment area were associated with a higher abundance of potential opportunistic pathogens and higher concentrations of QACs. 相似文献
166.
167.
Raidl M Sibbing B Strauch J Müller K Nemat A Schneider PM Hag H Erdmann E Koch A 《Cell biochemistry and biophysics》2007,49(2):98-110
The cytokine and potent angiogenic factor vascular endothelial growth factor (VEGF) plays an important role in airway remodelling
in various airway diseases such as idiopathic pulmonary fibrosis, pulmonary hypertension, lung cancer, asthma and chronic
obstructive pulmonary disease (COPD). The effect of cigarette-smoking on VEGF expression, the modulatory role of extracellular
signal-regulated kinase (ERK)-1,-2, p38mitogen-activated protein kinase (MAPK), histone acetylation and the anti-inflammatory
effect of dexamethasone on TNFα-induced VEGF expression were examined in human airway smooth muscle cells (HASMC) of five
non-smokers, 17 smokers without airflow limitation and 15 smokers with COPD. TNFα increased VEGF expression 5.4-fold and 4.0-fold
in HASMC from non-smokers and smokers without airflow limitation, respectively, but only 2.5-fold in HASMC from smokers with
COPD compared with non-stimulated HASMC. VEGF production was dependent on phosphorylation of ERK-1,-2 and p38MAPK, as was shown by examining the effects of PD 098059 (10 μM), an inhibitor of the upstream activator of MAPKkinase (MKK)-1,
and SB 203580 (10 μM), an inhibitor of p38MAPK; there were no differences between non-smokers, smokers without airflow limitation and smokers with COPD in this respect.
Dexamethasone (DEX; 10−12–10−4 M) reduced TNFα-induced phosphorylation of ERK-1/-2 and prevented TNFα-induced VEGF generation without differences between
non-smokers, smokers with and without COPD. There was an additional inhibitory effect of DEX (10−12 M) on VEGF-release when PD 098059 was added. The basal and TNFα-induced acetylation status of the VEGF-promoter (chromatin
immunoprecipitation [ChIP] assay) was increased in HASMC from smokers with COPD compared with smokers without airflow limitation
and non-smokers. In comparison to non-stimulated HASMC, TNFα decreased the acetylation status of the VEGF-promoter by ∼46%
and ∼43% in HASMC from non-smokers and smokers without COPD compared with ∼68% in HASMC from smokers with COPD. The data suggest
that HASMC express VEGF in response to TNFα and that this may be reduced in HASMC of smokers with COPD in a smoking-independent
manner. VEGF expression is directly modulated by phosphorylation of ERK-1,-2 and p38MAPK and by histone acetylation and the acetylation status of the VEGF gene is increased in HASMC of smokers with COPD in a smoking-independent
manner. TNFα reduced the acetylation status of the VEGF promoter in HASMC. 相似文献
168.
Garrick P. Smith Lassina Badolo Victoria Chell I-Jen Chen Kenneth Vielsted Christensen Laurent David Justus Alfred Daechsel Morten Hentzer Martin Christian Herzig Gitte Kobberøe Mikkelsen Stephen P. Watson Douglas S. Williamson 《Bioorganic & medicinal chemistry letters》2017,27(18):4500-4505
Leucine-rich repeat kinase 2 (LRRK2) has attracted considerable interest as a therapeutic target for the treatment of Parkinson’s disease. Compounds derived from a 2-aminopyridine screening hit were optimised using a LRRK2 homology model based on mixed lineage kinase 1 (MLK1), such that a 2-aminopyridine-based lead molecule 45, with in vivo activity, was identified. 相似文献
169.
Justus F. Mueller 《Cell and tissue research》1931,12(3):436-450
170.