全文获取类型
收费全文 | 6254篇 |
免费 | 648篇 |
国内免费 | 6篇 |
出版年
2023年 | 41篇 |
2022年 | 103篇 |
2021年 | 198篇 |
2020年 | 99篇 |
2019年 | 152篇 |
2018年 | 171篇 |
2017年 | 121篇 |
2016年 | 239篇 |
2015年 | 377篇 |
2014年 | 402篇 |
2013年 | 407篇 |
2012年 | 556篇 |
2011年 | 563篇 |
2010年 | 313篇 |
2009年 | 275篇 |
2008年 | 359篇 |
2007年 | 380篇 |
2006年 | 293篇 |
2005年 | 271篇 |
2004年 | 221篇 |
2003年 | 190篇 |
2002年 | 175篇 |
2001年 | 87篇 |
2000年 | 106篇 |
1999年 | 76篇 |
1998年 | 50篇 |
1997年 | 39篇 |
1996年 | 35篇 |
1995年 | 45篇 |
1994年 | 29篇 |
1993年 | 22篇 |
1992年 | 49篇 |
1991年 | 41篇 |
1990年 | 38篇 |
1989年 | 37篇 |
1988年 | 36篇 |
1987年 | 32篇 |
1986年 | 31篇 |
1985年 | 29篇 |
1984年 | 20篇 |
1983年 | 10篇 |
1982年 | 23篇 |
1981年 | 12篇 |
1980年 | 16篇 |
1979年 | 14篇 |
1978年 | 8篇 |
1977年 | 10篇 |
1975年 | 12篇 |
1974年 | 9篇 |
1971年 | 6篇 |
排序方式: 共有6908条查询结果,搜索用时 437 毫秒
121.
Lauren F. Messer Martin Ostrowski Martina A. Doblin Katherina Petrou Mark E. Baird Timothy Ingleton Andrew Bissett Jodie Van de Kamp Tiffanie Nelson Ian Paulsen Levente Bodrossy Jed A. Fuhrman Justin R. Seymour Mark V. Brown 《Global Change Biology》2020,26(10):5613-5629
Western boundary currents (WBCs) redistribute heat and oligotrophic seawater from the tropics to temperate latitudes, with several displaying substantial climate change‐driven intensification over the last century. Strengthening WBCs have been implicated in the poleward range expansion of marine macroflora and fauna, however, the impacts on the structure and function of temperate microbial communities are largely unknown. Here we show that the major subtropical WBC of the South Pacific Ocean, the East Australian Current (EAC), transports microbial assemblages that maintain tropical and oligotrophic (k‐strategist) signatures, to seasonally displace more copiotrophic (r‐strategist) temperate microbial populations within temperate latitudes of the Tasman Sea. We identified specific characteristics of EAC microbial assemblages compared with non‐EAC assemblages, including strain transitions within the SAR11 clade, enrichment of Prochlorococcus, predicted smaller genome sizes and shifts in the importance of several functional genes, including those associated with cyanobacterial photosynthesis, secondary metabolism and fatty acid and lipid transport. At a temperate time‐series site in the Tasman Sea, we observed significant reductions in standing stocks of total carbon and chlorophyll a, and a shift towards smaller phytoplankton and carnivorous copepods, associated with the seasonal impact of the EAC microbial assemblage. In light of the substantial shifts in microbial assemblage structure and function associated with the EAC, we conclude that climate‐driven expansions of WBCs will expand the range of tropical oligotrophic microbes, and potentially profoundly impact the trophic status of temperate waters. 相似文献
122.
Alfredo Floristn Leah Morales Douglas Hanniford Carlos Martinez Elena Castellano‐Sanz Igor Dolgalev Alejandro Ulloa‐Morales Eleazar Vega‐Saenz de Miera Una Moran Farbod Darvishian Iman Osman Tomas Kirchhoff Eva Hernando 《Pigment cell & melanoma research》2020,33(3):466-479
Next‐generation sequencing has enabled genetic and genomic characterization of melanoma to an unprecedent depth. However, the high mutational background plus the limited depth of coverage of whole‐genome sequencing performed on cutaneous melanoma samples make the identification of novel driver mutations difficult. We sought to explore the somatic mutation portfolio in exonic and gene regulatory regions in human melanoma samples, for which we performed targeted sequencing of tumors and matched germline DNA samples from 89 melanoma patients, identifying known and novel recurrent mutations. Two recurrent mutations found in the RPS27 promoter associated with decreased RPS27 mRNA levels in vitro. Data mining and IHC analyses revealed a bimodal pattern of RPS27 expression in melanoma, with RPS27‐low patients displaying worse prognosis. In vitro characterization of RPS27‐high and RPS27‐low melanoma cell lines, as well as loss‐of‐function experiments, demonstrated that high RPS27 status provides increased proliferative and invasive capacities, while low RPS27 confers survival advantage in low attachment and resistance to therapy. Additionally, we demonstrate that 10 other cancer types harbor bimodal RPS27 expression, and in those, similarly to melanoma, RPS27‐low expression associates with worse clinical outcomes. RPS27 promoter mutation could thus represent a mechanism of gene expression modulation in melanoma patients, which may have prognostic and predictive implications. 相似文献
123.
124.
Hanisch Justin R. Connor Stephanie J. Scrimgeour Garry J. Cobbaert Danielle 《Wetlands Ecology and Management》2020,28(2):199-216
Wetlands Ecology and Management - We compared a rapid bioassessment protocol (Traveling Sweep Approach [TSA]) with a more conventional time intensive protocol (Composite Transect Approach [CTA]) to... 相似文献
125.
Popper Arthur N. Hawkins Anthony D. Jacobs Fred Jacobson Paul T. Johnson Peter Krebs Justin 《Reviews in Fish Biology and Fisheries》2020,30(4):605-622
Reviews in Fish Biology and Fisheries - Downstream movements of some freshwater fishes, including eels, are adversely affected by the presence of hydroelectric structures and other anthropic... 相似文献
126.
Transgenic Research - To inform the ecological risk assessment (ERA) of a transgenic crop with multiple insecticidal traits combined by conventional breeding (breeding stack), a comparative field... 相似文献
127.
Christian Kiffner John Kioko Jack Baylis Camille Beckwith Craig Brunner Christine Burns Vasco Chavez‐Molina Sara Cotton Laura Glazik Ellen Loftis Megan Moran Caitlin O'Neill Ole Theisinger Bernard Kissui 《Ecology and evolution》2020,10(18):10000-10016
Facilitating coexistence between people and wildlife is a major conservation challenge in East Africa. Some conservation models aim to balance the needs of people and wildlife, but the effectiveness of these models is rarely assessed. Using a case‐study approach, we assessed the ecological performance of a pastoral area in northern Tanzania (Manyara Ranch) and established a long‐term wildlife population monitoring program (carried out intermittently from 2003 to 2008 and regularly from 2011 to 2019) embedded in a distance sampling framework. By comparing density estimates of the road transect‐based long‐term monitoring to estimates derived from systematically distributed transects, we found that the bias associated with nonrandom placement of transects was nonsignificant. Overall, cattle and sheep and goat reached the greatest densities and several wildlife species occurred at densities similar (zebra, wildebeest, waterbuck, Kirk's dik‐dik) or possibly even greater (giraffe, eland, lesser kudu, Grant's gazelle, Thomson's gazelle) than in adjacent national parks in the same ecosystem. Generalized linear mixed models suggested that most wildlife species (8 out of 14) reached greatest densities during the dry season, that wildlife population densities either remained constant or increased over the 17‐year period, and that herbivorous livestock species remained constant, while domestic dog population decreased over time. Cross‐species correlations did not provide evidence for interference competition between grazing or mixed livestock species and wildlife species but indicate possible negative relationships between domestic dog and warthog populations. Overall, wildlife and livestock populations in Manyara Ranch appear to coexist over the 17‐year span. Most likely, this is facilitated by existing connectivity to adjacent protected areas, effective anti‐poaching efforts, spatio‐temporal grazing restrictions, favorable environmental conditions of the ranch, and spatial heterogeneity of surface water and habitats. This long‐term case study illustrates the potential of rangelands to simultaneously support wildlife conservation and human livelihood goals if livestock grazing is restricted in space, time, and numbers. 相似文献
128.
Justin C. Bagley Neander M. Heming Elicer E. Gutirrez Upendra K. Devisetty Karen E. Mock Andrew J. Eckert Steven H. Strauss 《Ecology and evolution》2020,10(11):4609-4629
Populus tremuloides is the widest‐ranging tree species in North America and an ecologically important component of mesic forest ecosystems displaced by the Pleistocene glaciations. Using phylogeographic analyses of genome‐wide SNPs (34,796 SNPs, 183 individuals) and ecological niche modeling, we inferred population structure, ploidy levels, admixture, and Pleistocene range dynamics of P. tremuloides, and tested several historical biogeographical hypotheses. We found three genetic lineages located mainly in coastal–Cascades (cluster 1), east‐slope Cascades–Sierra Nevadas–Northern Rockies (cluster 2), and U.S. Rocky Mountains through southern Canadian (cluster 3) regions of the P. tremuloides range, with tree graph relationships of the form ((cluster 1, cluster 2), cluster 3). Populations consisted mainly of diploids (86%) but also small numbers of triploids (12%) and tetraploids (1%), and ploidy did not adversely affect our genetic inferences. The main vector of admixture was from cluster 3 into cluster 2, with the admixture zone trending northwest through the Rocky Mountains along a recognized phenotypic cline (Utah to Idaho). Clusters 1 and 2 provided strong support for the “stable‐edge hypothesis” that unglaciated southwestern populations persisted in situ since the last glaciation. By contrast, despite a lack of clinal genetic variation, cluster 3 exhibited “trailing‐edge” dynamics from niche suitability predictions signifying complete northward postglacial expansion. Results were also consistent with the “inland dispersal hypothesis” predicting postglacial assembly of Pacific Northwestern forest ecosystems, but rejected the hypothesis that Pacific‐coastal populations were colonized during outburst flooding from glacial Lake Missoula. Overall, congruent patterns between our phylogeographic and ecological niche modeling results and fossil pollen data demonstrate complex mixtures of stable‐edge, refugial locations, and postglacial expansion within P. tremuloides. These findings confirm and refine previous genetic studies, while strongly supporting a distinct Pacific‐coastal genetic lineage of quaking aspen. 相似文献
129.
Tessa A. Rhinehart Lauren M. Chronister Trieste Devlin Justin Kitzes 《Ecology and evolution》2020,10(13):6794-6818
Autonomous acoustic recorders are an increasingly popular method for low‐disturbance, large‐scale monitoring of sound‐producing animals, such as birds, anurans, bats, and other mammals. A specialized use of autonomous recording units (ARUs) is acoustic localization, in which a vocalizing animal is located spatially, usually by quantifying the time delay of arrival of its sound at an array of time‐synchronized microphones. To describe trends in the literature, identify considerations for field biologists who wish to use these systems, and suggest advancements that will improve the field of acoustic localization, we comprehensively review published applications of wildlife localization in terrestrial environments. We describe the wide variety of methods used to complete the five steps of acoustic localization: (1) define the research question, (2) obtain or build a time‐synchronizing microphone array, (3) deploy the array to record sounds in the field, (4) process recordings captured in the field, and (5) determine animal location using position estimation algorithms. We find eight general purposes in ecology and animal behavior for localization systems: assessing individual animals' positions or movements, localizing multiple individuals simultaneously to study their interactions, determining animals' individual identities, quantifying sound amplitude or directionality, selecting subsets of sounds for further acoustic analysis, calculating species abundance, inferring territory boundaries or habitat use, and separating animal sounds from background noise to improve species classification. We find that the labor‐intensive steps of processing recordings and estimating animal positions have not yet been automated. In the near future, we expect that increased availability of recording hardware, development of automated and open‐source localization software, and improvement of automated sound classification algorithms will broaden the use of acoustic localization. With these three advances, ecologists will be better able to embrace acoustic localization, enabling low‐disturbance, large‐scale collection of animal position data. 相似文献
130.
Andrew L. Von Duyke David C. Douglas Jason K. Herreman Justin A. Crawford 《Ecology and evolution》2020,10(12):5595-5616
Continued Arctic warming and sea‐ice loss will have important implications for the conservation of ringed seals, a highly ice‐dependent species. A better understanding of their spatial ecology will help characterize emerging ecological trends and inform management decisions. We deployed satellite transmitters on ringed seals in the summers of 2011, 2014, and 2016 near Utqia?vik (formerly Barrow), Alaska, to monitor their movements, diving, and haul‐out behavior. We present analyses of tracking and dive data provided by 17 seals that were tracked until at least January of the following year. Seals mostly ranged north of Utqia?vik in the Beaufort and Chukchi Seas during summer before moving into the southern Chukchi and Bering Seas during winter. In all seasons, ringed seals occupied a diversity of habitats and spatial distributions, from near shore and localized, to far offshore and wide‐ranging in drifting sea ice. Continental shelf waters were occupied for >96% of tracking days, during which repetitive diving (suggestive of foraging) primarily to the seafloor was the most frequent activity. From mid‐summer to early fall, 12 seals made ~1‐week forays off‐shelf to the deep Arctic Basin, most reaching the retreating pack‐ice, where they spent most of their time hauled out. Diel activity patterns suggested greater allocation of foraging efforts to midday hours. Haul‐out patterns were complementary, occurring mostly at night until April‐May when midday hours were preferred. Ringed seals captured in 2011—concurrent with an unusual mortality event that affected all ice‐seal species—differed morphologically and behaviorally from seals captured in other years. Speculations about the physiology of molting and its role in energetics, habitat use, and behavior are discussed; along with possible evidence of purported ringed seal ecotypes. 相似文献