FIBONACCI级数与菊花结构规律的研究

本文论证了菲波纳斯（Fibonacci）级数与菊花花冠结构的关系,发现了花冠的通用数学模式：花冠（数目）＝（5×倍数）十F_n（F_(n－1)＋F_(n-2)）     

中国羽枝藻属新资料

1.山西羽枝藻 新种 图1:1—4 Cloniophora shanxiensis Y. J. Ling et S. L. Xie, sp. nov. Species affnis C. spicatae (Schmid.) Islam, sed primariis ramulis irregularibus alternatisvel oppositis, pyrenoidibus solitariis, ramuli cellulis apprime irregularibus differt. Thallo caespitoso, usque ad 5 cm longo; rhizoideis e filamenti cellulis basalibus angustis     

天然杀伤细胞在受照射小鼠中的造血调控作用

研究了天然杀伤（ＮＫ）细胞对受致死剂量γ线照射的同系小鼠的造血调控作用。ＡＭＳ／５小鼠经９Ｇｙγ线全身照射后立即经尾静脉注射ＮＫ细胞（５×１０５）,可明显提高受照小鼠３０ｄ活存率,照后８ｄ小鼠骨髓中ＣＦＵ－ＧＭ数量明显高于对照和脾细胞注射组,照射后３０ｄ,ＮＫ细胞注射组活存小鼠的骨髓有核细胞数和ＣＦＵ－ＧＭ数已恢复到正常的７６％─９６％。病理组织学观察显示,输注ＮＫ细胞可使小鼠骨髓、脾脏的组织损伤程度减轻,造血功能增强,表现为造血灶数增多,造血细胞功能活跃,核分裂相增多,且涉及红系、粒系、巨核细胞系造血。ＮＫ细胞可能通过直接与造血干细胞相互作用或改善造血微环境等促进“内源性”造血功能,从而发挥对造血的正调控作用。提示ＮＫ细胞在小鼠造血功能的平衡维持中起重要作用。     

自然免耕下的稻田生态系统

自然免耕是一种新型的稻田耕作法,通过人为改变地表微地形,建立了一个适合水旱作物复种轮作,鱼、萍、鸭周年共生的稻田生态系统．自然免耕促进了土壤内、外环境的物质、能量和信息交换,水、热、气、肥谐调．并通过环境网络效应,强化了环境与生物区系间的缓冲－调节力．提高了土壤自调能力和土壤有序度．     

Surface imprinted bio-nanocomposites for affinity separation of a cellular DNA repair protein

Apurinic/apyrimidinic endonuclease 1 (APE1) is a multifunctional DNA repair protein localized in different subcellular compartments. The mechanisms responsible for the highly regulated subcellular localization and “interactomes” of this protein are not fully understood but have been closely correlated to the posttranslational modifications in different biological context. In this work, we attempted to develop a bio-nanocomposite with antibody-like properties that could capture APE1 from cellular matrices to enable the comprehensive study of this protein. By fixing the template APE1 on the avidin-modified surface of silica-coated magnetic nanoparticles, we first added 3-aminophenylboronic acid to react with the glycosyl residues of avidin, followed by addition of 2-acrylamido-2-methylpropane sulfonic acid as the second functional monomer to perform the first step imprinting reaction. To further enhance the affinity and selectivity of the binding sites, we carried out the second step imprinting reaction with dopamine as the functional monomer. After the polymerization, we modified the nonimprinted sites with methoxypoly (ethylene glycol) amine (mPEG-NH₂). The resulting molecularly imprinted polymer-based bio-nanocomposite showed high affinity, specificity, and capacity for template APE1. It allowed for the extraction of APE1 from the cell lysates with high recovery and purity. Moreover, the bound protein could be effectively released from the bio-nanocomposite with high activity. The bio-nanocomposite offers a very useful tool for the separation of APE1 from various complex biological samples.     

Regulation of Nitrogen Metabolism,Photosynthetic Activity,and Yield Attributes of Spring Wheat by Nitrogen Fertilizer in the Semi-arid Loess Plateau Region

It is critical for spring wheat (Triticum aestivum L.) production in the semi-arid Loess Plateau to understand the impact of nitrogen (N) fertilizer on changes in N metabolism, photosynthetic parameters, and their relationship with grain yield and quality. The photosynthetic capacity of flag leaves, dry matter accumulation, and N metabolite enzyme activities from anthesis to maturity were studied on a long-term fertilization trial under different N rates [0 kg ha^?1(N1), 52.5 kg ha^?1 (N2), 105 kg ha^?1 (N3), 157.5 kg ha^?1 (N4), and 210 kg ha^?1 (N5)]. It was observed that N3 produced optimum total dry matter (5407 kg ha^?1), 1000 grain weight (39.7 g), grain yield (2.64 t ha^?1), and protein content (13.97%). Our results showed that N fertilization significantly increased photosynthetic parameters and N metabolite enzymes at all growth stages. Nitrogen harvest index, partial productivity factor, agronomic recovery efficiency, and nitrogen agronomic efficiency were decreased with increased N. Higher N rates (N3–N5) maintained higher photosynthetic capacity and dry matter accumulation and lower intercellular CO₂ content. The N supply influenced NUE by improving photosynthetic properties. The N3 produced highest chlorophyll content, photosynthetic rate, stomatal conductance and transpiration rate, grain yield, grain protein, dry matter, grains weight, and N metabolite enzyme activities compared to the other rates (N1, N2, N4, and N5). Therefore, increasing N rates beyond the optimum quantity only promotes vegetative development and results in lower yields.

     

异育银鲫及其人工杂合种外源遗传物质的检测分析

采用 DNA 杂交的方法,对异育银鲫及其人工杂合种进行了外源 DNA 的检测分析,发现两个雌核发育系的异育银鲫及其人工杂合种的 DNA 与红鲤 DNA 片段间的分子杂交均有阳性斑点出现,这表明,在异育银鲫及其人工杂合种产生过程中,父本(红鲤)的 DNA 片段的确可以随机地掺入到母本细胞的 DNA 中,从而产生了异精效应,使其子代生长加快,并出现父本性状。     

在吲哚乙酸不同位点偶联载体蛋白对其抗体特异性的影响

分别选择吲哚乙醇分子上的Ｃ１位羧基和吲哚环上的Ｎ位作为偶联载体蛋白的位点,用混合酸酐法和甲醛搭桥法分别合成了两种免疫原ＩＡＡ—ＣＯ—ＮＨ一ＨＳＡ和ＩＡＡ－Ｎ－ＢＳＡ,并进而制得了对吲哚乙酸侧链识别能力不同的两种多克隆抗体,分别可特异识别甲酯化ＩＡＡ和游离态ＩＡＡ;用碳化二亚胺法和甲醛搭桥法分别合成ＩＡＡ—ＣＯ—ＮＨ－ＢＳＡｂＩＡＡ—Ｎ—ＯＶＡ两种复合物,以之为包被物,建立了两种ＩＡＡＥＬＩＳＡ。其灵敏度分别为０．３５ｐｍｏｌ和１．８０ｐｐｍｏｌ;检测范围分别为０．７８～８００ｐｍｏｌ和１．９５～２０００ｐｍｏｌ;批内变异系数分别为４．４５％和４．７９％;批间变异系数分别为１．１５％和１．５０％。笔者用这两种ＥＬＩＳＡ检测了兰花气生根和桑树苗样品中ＩＡＡ的含量,发现两种检测结果相当一致。