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AcMNPV核衣壳的形态发生 总被引:2,自引:0,他引:2
报道了缺失多角体蛋白基因的AcMNPV在sf9细胞内核衣壳的形态发生过程。病毒衣壳蛋白首先装配成许多呈束状排列的直径为34nm中空长管状结构,然后是病毒DNA进入管内,装有DNA的长管按一定的长度间隔断开,形成成束的核衣壳,每个核衣壳的大小约34×260nm,最后成束的核衣壳被囊膜包被形成完整的多粒包埋型病毒粒子。 相似文献
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Li Junlin Li Zhifei Xie Jun Xia Yun Gong Wangbao Tian Jingjing Zhang Kai Yu Ermeng Wang Guangjun 《International microbiology》2023,26(3):639-650
International Microbiology - Quorum sensing (QS) is a core mechanism for bacteria to regulate biofilm formation, and therefore, QS inhibition or quorum quenching (QQ) is used as an effective and... 相似文献
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Large molecular weight plasmids are often used in gene therapy and DNA vaccines. To investigate the effect of plasmid size on the performance of Escherichia coli host strains during plasmid preparation, we employed E. coli JM109 and TOP10 cells to prepare four plasmids ranging from 4.7 to 16.8?kb in size. Each plasmid was extracted from JM109 and TOP10 cells using an alkaline lysis mini-preparation method. However, when commercial kits were used to extract the same plasmids from JM109 cells, the large molecular weight plasmids substantially degraded, compared with their smaller counterparts. No degradation was observed when the four plasmids were extracted from E. coli TOP10 cells using the same commercial kit. We conclude, therefore, that the performance of E. coli in high quality plasmid preparations can be affected by plasmid size. 相似文献
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Q Wang P Schwarzenberger F Yang J Zhang J Su C Yang J Cao C Ou L Liang J Shi F Yang D Wang J Wang X Wang P Ruan Y Li 《Virology journal》2012,9(1):170
ABSTRACT: BACKGROUND: Hepatitis B virus (HBV) infection continues to be an escalating global health problem. Feasible and effective animal models for HBV infection are the prerequisite for developing novel therapies for this disease. The tree shrew (Tupaia) is a small animal species evolutionary closely related to humans, and thus is permissive to certain human viral pathogens. Whether tree shrews could be chronically infected with HBV in vivo has been controversial for decades. Most published research has been reported on adult tree shrews, and only small numbers of HBV infected newborn tree shrews had been observed over short time periods. We investigated susceptibility of newborn tree shrews to experimental HBV infection as well as viral clearance over a protracted time period. RESULTS: Forty-six newborn tree shrews were inoculated with the sera from HBV-infected patients or tree shrews. Serum and liver samples of the inoculated animals were periodically collected and analyzed using fluorescence quantitative polymerase chain reaction, enzyme-linked immunosorbent assay, Southern blot, and immunohistochemistry. Six tree shrews were confirmed and four were suspected as chronically HBV-infected for more than 48 (up to 228) weeks after inoculation, including three that had been inoculated with serum from a confirmed HBV-infected tree shrew. CONCLUSIONS: Outbred neonatal tree shrews can be long-term chronically infected with HBV at a frequency comparable to humans. The model resembles human disease where also a smaller proportion of infected individuals develop chronic HBV related disease. This model might enable genetic and immunologic investigations which would allow determination of underlying molecular causes favoring susceptibility for chronic HBV infection and disease establishment vs. viral clearance. 相似文献
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Junlin Pan YuanYuan Yao Xiuxiu Guo Fengyun Kong Jun Zhou Xiaoqian Meng 《Journal of cellular physiology》2019,234(11):19807-19823
Di(2-ethylhexyl) phthalate (DEHP), a widely existed endocrine disruptor, has been concerned for many years owing to its toxicity in male reproductive development. In this study, we investigated the reproductive effects and the mechanism of mouse testis after in uterus exposure to the plasticizer DEHP. We found that the UPR signaling pathway could be fully activated after DEHP treatment. In uterus DEHP exposure significantly increased abnormal morphology seminiferous tubules, expanded the distance between the tubules as well as caused abnormal endoplasmic reticulum (ER) ultrastructure, which could be reversed by 4-phenylbutyrate (4-PBA), an ER stress inhibitor. In addition, DEHP-induced ER stress pathway promoted a decline in protein expression, including cadherin protein N-cadherin in testis, which could also be reversed by 4-PBA. Taken together, our results provide compelling evidence that the ER stress would be a novel significant mechanism responsible for DEHP-induced the increased the distance between seminiferous tubule by reducing the N-cadherin expression. 相似文献
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Yali Zhou Henrik Daver Boris Trapkov Lijie Wu Meng Wu Kasper Harps?e Patrick R.Gentry Kaiwen Liu Marina Larionova Junlin Liu Na Chen Hans Bruner-Osborne David E.Gloriam Tian Hua Zhi-Jie Liu 《Cell research》2022,(2):210-213
Dear Editor,
The G protein-coupled receptor GPR139 is involved in neuro-modulation,and one of its agonists is in clinical trials for the treatment of cognitive ... 相似文献
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Sili Zou Mingfang Liao Junlin Yang Tong Huang Mark Green Jianjin Wu Lefeng Qu 《Cellular & molecular biology letters》2017,22(1):24