全文获取类型
收费全文 | 1639篇 |
免费 | 84篇 |
出版年
2022年 | 13篇 |
2021年 | 26篇 |
2020年 | 4篇 |
2019年 | 26篇 |
2018年 | 16篇 |
2017年 | 12篇 |
2016年 | 35篇 |
2015年 | 47篇 |
2014年 | 77篇 |
2013年 | 78篇 |
2012年 | 115篇 |
2011年 | 110篇 |
2010年 | 64篇 |
2009年 | 53篇 |
2008年 | 131篇 |
2007年 | 93篇 |
2006年 | 94篇 |
2005年 | 94篇 |
2004年 | 114篇 |
2003年 | 104篇 |
2002年 | 108篇 |
2001年 | 16篇 |
2000年 | 12篇 |
1999年 | 22篇 |
1998年 | 24篇 |
1997年 | 16篇 |
1996年 | 12篇 |
1995年 | 15篇 |
1994年 | 17篇 |
1993年 | 17篇 |
1992年 | 12篇 |
1991年 | 14篇 |
1990年 | 7篇 |
1989年 | 10篇 |
1988年 | 11篇 |
1987年 | 9篇 |
1986年 | 3篇 |
1985年 | 6篇 |
1984年 | 8篇 |
1983年 | 12篇 |
1982年 | 15篇 |
1981年 | 8篇 |
1980年 | 8篇 |
1979年 | 8篇 |
1978年 | 4篇 |
1977年 | 5篇 |
1975年 | 3篇 |
1974年 | 3篇 |
1972年 | 2篇 |
1971年 | 3篇 |
排序方式: 共有1723条查询结果,搜索用时 15 毫秒
71.
Recent advances in the use of summed probability distribution (SPD) of calibrated 14C dates have opened new possibilities for studying prehistoric demography. The degree of correlation between climate change and population dynamics can now be accurately quantified, and divergences in the demographic history of distinct geographic areas can be statistically assessed. Here we contribute to this research agenda by reconstructing the prehistoric population change of Jomon hunter-gatherers between 7,000 and 3,000 cal BP. We collected 1,433 14C dates from three different regions in Eastern Japan (Kanto, Aomori and Hokkaido) and established that the observed fluctuations in the SPDs were statistically significant. We also introduced a new non-parametric permutation test for comparing multiple sets of SPDs that highlights point of divergences in the population history of different geographic regions. Our analyses indicate a general rise-and-fall pattern shared by the three regions but also some key regional differences during the 6th millennium cal BP. The results confirm some of the patterns suggested by previous archaeological studies based on house and site counts but offer statistical significance and an absolute chronological framework that will enable future studies aiming to establish potential correlation with climatic changes. 相似文献
72.
Yamanaka Akihiro Beuckmann Carsten T. Willie Jon T. Hara Junko Tsujino Natsuko Goto Katsutoshi Yanagisawa Masashi Sakurai Takeshi 《Sleep and biological rhythms》2016,2(1):S57-S57
Sleep and Biological Rhythms - 相似文献
73.
Akihiro Ohyama Junko Toyomura Toshiaki Tachibana Seiji Isonishi Haruka Takahashi Hiroshi Ishikawa 《Human cell》2016,29(4):188-196
A cell line, designated NOCC, was established from the ascites of a patient with clear cell adenocarcinoma of the ovary. The cell line has been grown without interruption and continuously propagated by serial passaging (more than 76 times) over 7 years. The cells are spherical to polygonal-shaped, display neoplastic, and pleomorphic features, and grow in a jigsaw puzzle-like pattern while forming monolayers without contact inhibition. The cells proliferate rapidly, but are easily floated as a cell sheet. The population doubling time is about 29 h. The number of chromosomes ranges from 60 to 83. The modal number of chromosomes is 70–74 at the 30th passage. NOCC cells secreted 750.5 ng/ml of VEGF over 3 days of culture. Hypoxia inducible factor-1α (HIF-1α) is a primary regulator of VEGF under hypoxic conditions. NOCC cells were not sensitive to the anticancer drugs BEV, DOX, GEM, ETP, CDDP, or TXT. The graft of NOCC cells to a scid mouse displayed similar histological aspects to the original tumor. Both the NOCC cells and the graft of the NOCC cells gave a positive PAS reaction. 相似文献
74.
Yoshinori Yabuki Takashi Nagai Keiya Inao Junko Ono Nobuyuki Aiko Nobutoshi Ohtsuka 《Bioscience, biotechnology, and biochemistry》2016,80(10):2069-2075
Laboratory experiments were performed to determine the sampling rates of pesticides for the polar organic chemical integrative samplers (POCIS) used in Japan. The concentrations of pesticides in aquatic environments were estimated from the accumulated amounts of pesticide on POCIS, and the effect of water temperature on the pesticide sampling rates was evaluated. The sampling rates of 48 pesticides at 18, 24, and 30 °C were obtained, and this study confirmed that increasing trend of sampling rates was resulted with increasing water temperature for many pesticides. 相似文献
75.
Cigarette smoking alters sialylation in the Fallopian tube of women,with implications for the pathogenesis of ectopic pregnancy
下载免费PDF全文
![点击此处可从《Molecular reproduction and development》网站下载免费的PDF全文](/ch/ext_images/free.gif)
76.
77.
78.
79.
80.
Yukinari Kato Yoshikazu Furusawa Shinji Yamada Shunsuke Itai Junko Takei Masato Sano Mika K. Kaneko 《Biochemistry and Biophysics Reports》2019
Podoplanin (PDPN) is known as a lymphatic endothelial cell marker. Monoclonal antibodies (mAbs) against human, mouse, rat, rabbit, dog, cat, bovine, pig, and horse PDPN have been established in our previous studies. However, mAbs against alpaca PDPN (aPDPN), required for immunohistochemical analysis, remain to be developed. In the present study, we employed the Cell-Based Immunization and Screening (CBIS) method for producing anti-aPDPN mAbs. We immunized mice with aPDPN-overexpressing Chinese hamster ovary (CHO)-K1 cells (CHO/aPDPN), and hybridomas producing mAbs against aPDPN were screened using flow cytometry. One of the mAbs, PMab-225 (IgG2b, kappa), specifically detected CHO/aPDPN cells via flow cytometry and recognized the aPDPN protein on Western blotting. Further, PMab-225 strongly stained lung type I alveolar cells, colon lymphatic endothelial cells, and kidney podocytes via immunohistochemistry. These findings demonstrate that PMab-225 antibody is useful to investigate the function of aPDPN via different techniques. 相似文献