第一类鱼抗冻蛋白对冰晶生长界面层结构的影响

根据冰晶在水溶液中生长的基本热力学性质,应用多层界面模型,分别得到了冰晶在纯水及抗冻蛋白溶液中生长界面层的吉布斯自由能.由冰晶生长界面层的吉布斯自由能,分析了冰晶在三种不同第一类鱼抗冻蛋白分子溶液中,热平衡状态下生长界面层的微观平衡结构,发现冰晶在抗冻蛋白溶液中生长与其在纯水中生长相比,界面层结构有明显变化,结合抗冻蛋...     

A novel matrix derivatized from hydrophilic gigaporous polystyrene-based microspheres for high-speed immobilized-metal affinity chromatography

Agarose coated gigaporous polystyrene microspheres were evaluated as a novel matrix for immobilized-metal affinity chromatography (IMAC). With four steps, nickel ions were successfully immobilized on the microspheres. The gigaporous structure and chromatographic properties of IMAC medium were characterized. A column packed with the matrix showed low column backpressure and high column efficiency at high flow velocity. Furthermore, this matrix was used for purifying superoxide dismutase (SOD), which was expressed in Escherichia coli (E. coli) in submerged fermentation, on an Äkta purifier 100 system under different flow velocities. The purity of the SOD from this one-step purification was 79% and the recovery yield was about 89.6% under the superficial flow velocity of 3251 cm/h. In conclusion, all the results suggested that the gigaporous matrix has considerable advantages for high-speed immobilized-metal affinity chromatography.     

Comparative Effects of Hispidulin,Genistein, and Icariin with Estrogen on Bone Tissue in Ovariectomized Rats

Icariin, Genistein, and Hispidulin have been proven to have estrogen-like and antiosteoporotic activity and can be potentially used for the treatment of osteoporosis. The present study found that Icariin, Genistein, and Hispidulin treatments, emulating estrogen, significantly contributed to bone density. Comparative effects of Icariin, Genistein, and Hispidulin with estrogen on in ovariectomized rats were investigated. Our results showed that genistein was found to have superior bone protective effects against osteoporosis among genistein, Icariin, and Hispidulin.     

用重组大肠杆菌JM109（pKST11）转化苯生产顺-1,2-二羟基-3，5-环己二烯

顺-1,2-二羟基-3,5-环己二烯（简称DHCD）是航天业,电子工业,医药业以及精细化工业上重要的手性化合物,利用重组E.coli JM109（pKST11）,采用适时监测发酵过程中全细胞甲苯双加氧酶（Toluene dioxygenase,TDO）活性的方法,研究了发酵生产DHCD工艺中的重要影响因子IPTG以及底物苯的供给方式对DHCD产量的影响,研究结果表明：（1）发酵初期利用IPTG诱导TDO的表达,不利于细胞生长,在对数生长中期（6或8h）,采用0.5mmol/L IPTG即可诱导出TDO的最高表达。（2）发酵液中的苯对全细胞甲苯双加氧酶（TDO）的活性有抑制作用,而利用液体石蜡作为缓释剂进行两相法发酵则降低了苯的毒害,明显提高了DHCD的产量。当采用传统的通气供苯方法,DHCD的产量仅有7.5g/L;批式添加液体石蜡与苯的混溶物使DHCD的产量提高到22.6g/L,是通气供苯法的3倍;而采用流加的方式添加液体石蜡与苯的混溶物使DHCD的产量进一步提高到36.8g/L,是通气供苯法的5倍,证明通过发酵工艺的优化可以解决苯的毒害与苯作为反应底物在水相中需要一定浓度之间的矛盾,获得较好的转化结果。     

乳酸菌双组分信号转导系统研究进展

乳酸菌中存在着一种重要的调控机制--双组分信号转导系统,它可以通过调控乳酸菌的多种生理生化过程来适应外界环境的变化.就双组分信号转导系统的组成、作用机制以及乳酸菌中调控耐酸机制、细菌素的合成和黏性吸附等生理过程的双组分信号转导系统作一综述.     

荒漠沙蜥活动期间的体温节律

爬行动物的体温依赖于外界热环境的变化,本研究以荒漠沙蜥(Phrynocephalus przewalskii)为对象,收集其冬眠前活动期间的体温与地面和离地10 cm的空气温度,研究爬行动物体温节律性变化与环境温度的关系.研究结果表明,荒漠沙蜥在环境温度高于20℃后开始外出活动,其体温随着地面温度和空气温度的上升而逐步...     

色氨酸残基在内切葡聚糖酶分子中的作用

内切葡聚糖酶的化学修饰研究表明：色氨酸残基可能位于活性位点,与底物结合有关．荧光光谱测定指出该酶的荧光几乎都来自色氨酸残基,酶分子中色氨酸微环境对ｐＨ变化非常敏感,降低ｐＨ导致了酶分子构象发生了较大变化,配基结合使酶分子色氨酸微环境产生了改变,引发了与ｐＨ诱导不同的构象变化．