Gene microarray analysis of expression profiles in Suberoyllanilide hyroxamic acid-treated Dendritic cells

Objective

The purpose of this study is to provide a further theoretical basis for the role of Suberoyllanilide hyroxamic acid (SAHA) affect on Dendritic cells (DCs).

转Bt基因植物中外源基因时空动态表达的研究现状

在转Bt基因植株中 ,外源基因的时空动态表达对于害虫的防治和转基因安全评价管理具有重要意义。利用生物测定法和酶联免疫吸附测定法 (ELISA) ,对植物不同组织在同一发育阶段、同一组织在不同的发育阶段以及不同转基因植株的外源基因的时空动态表达进行研究。本文综述了转基因植物中外源基因时空动态表达的研究进展和现状。     

马铃薯晚疫病抗病基因研究进展

马铃薯晚疫病是马铃薯和番茄等茄科植物中最主要的病害之一,每年都引起巨大的经济损失。基因工程技术的发展为马铃薯晚疫病的防治工作提出了新的契机,从晚疫病抗性品种中筛选出具有高持久抗性的抗病基因,并将其转化到栽培品种中去,无疑是我们开发持久性晚疫病抗性的最快捷的手段。到目前为至,已经有十几个晚疫病抗性基因从S．demissum,S. bulbocastanum,S．berthauhii,S．mochiquense和S．pinnatisectum等抗性马铃薯品种中鉴定出来并已定位在马铃薯染色体基因组上,并有4个被克隆出来（R1,R3a,Rpi—blb1／RB和Rpi—blb2）。主要概述了马铃薯晚疫病抗病基因的研究现状和发展前景。     

湄洲湾潮间带底栖生物多样性

为了了解福建省湄洲湾潮间带底栖生物的物种多样性、数量时空分布及群落结构现状,2005年11月和2006年4月在湄洲湾潮间带选择了灵川(Mch1)、苏厝(Mch2)、郭厝(Mch3)、东吴(Mch4)和东桥(Mch5)5条断面进行了调查。共鉴定潮间带底栖生物225种,其中多毛类、软体动物和甲壳动物占总种数的88.00%,三者构成潮间带底栖生物的主要类群。断面间比较,东桥断面种数较多,苏厝断面较少。季节比较,春季各断面种类较秋季多。平均生物量22.91g/m2,以软体动物居第一位,甲壳动物居第二位;平均栖息密度为388个/m2,以多毛类居第一位,软体动物居第二位。生物量以中潮区>低潮区>高潮区;栖息密度以低潮区>中潮区>高潮区。调查结果显示湄洲湾潮间带底栖生物物种多样性较高,群落结构相对稳定。     

FAK contributes to proteinuria in hypercholesterolaemic rats and modulates podocyte F‐actin re‐organization via activating p38 in response to ox‐LDL

Focal adhesion kinase (FAK) is a non‐receptor protein tyrosine kinase that regulates cell adhesion, proliferation and differentiation. In the present study, a rat model of high fat diet‐induced hypercholesterolaemia was established to investigate the involvement of FAK in lipid disorder‐related kidney diseases. We showed focal fusion of podocyte foot process that occurred at as early as 4 weeks in rats consuming high fat diet, preceding the onset of proteinuria when aberrant phosphorylation of FAK was found. These abnormalities were ameliorated by dietary intervention of TAE226, a reported inhibitor of FAK. FAK is also an adaptor protein initiating cascades of intracellular signals including c‐Src, Rho GTPase and mitogen‐activated protein kinase (MAPK). P38 MAPK belongs to the latter and is centrally involved in kidney diseases. Our cell culture data revealed oxidized low‐density lipoprotein (ox‐LDL) triggered hyper‐phosphorylation of FAK and p38, ectopic expression of cellular markers (manifested as decreased WT1, podocin and NEPH1, and increased vimentin and mmp9), and re‐arrangement of F‐actin filaments with enhanced cell motility; these mutations were significantly rectified by FAK shRNA. Notably, pre‐treatment of p38 inhibitor did not alter FAK activation, albeit its deletion of p38 hyper‐activity and attenuation of cellular abnormalities, demonstrating that p38 acted as a downstream effector of FAK signalling and ox‐LDL damaged podocytes in a FAK/p38‐dependent manner. This was further identified by animal data that p38 activation was also abrogated by TAE226 treatment in hypercholesterolaemic rats, suggesting that FAK/p38 axis might also be involved in in vivo events. These findings provided a potential early mechanism of hypercholesterolaemia‐related podocyte damage and proteinuria.     

代谢工程改造L-半胱氨酸供给模块促地衣芽胞杆菌高效合成杆菌肽

杆菌肽是一种主要由芽胞杆菌产生的广谱性抗生素,目前作为兽药广泛应用于畜禽养殖领域.前体氨基酸供应不足可能是限制微生物发酵高产杆菌肽的重要因素.文中以杆菌肽工业生产菌株——地衣芽胞杆菌Bacillus licheniformis DW2为出发菌株,研究L-半胱氨酸供给模块强化对杆菌肽合成的影响.首先,构建了L-半胱氨酸合...     

C19orf66 Inhibits Japanese Encephalitis Virus Replication by Targeting -1 PRF and the NS3 Protein

Virologica Sinica - The Japanese encephalitis serogroup of the neurogenic Flavivirus has a specific feature that expresses a non-structural protein NS1′ produced through a programmed -1...     

A new species of Fuziidae (Insecta, Blattida) from the Inner Mongolia, China

A new species attributed to the genus Parvifuzia Guo & Ren, 2011, Parvifuzia peregrinasp. n., is described from the Middle Jurassic Jiulongshan Formation of Daohugou Village, Inner Mongolia, China. This new species, with apex of wing almost reaching the end of the abdomen and forewing venation with 30-32 veins at margin, broadens the diversity of Parvifuzia. This new species, with strongly curved cerci, could tightly clasp female and complete copulation more efficiently, same as other members of the family Fuziidae.     

三种提取RNA的方法对汉滩病毒检测敏感性影响的研究

分别采用标准法、ＧＰ法和ＭＢＰ法抽提汉滩病毒ＲＮＡ,结合套式ＰＣＲ比较这三种方法的敏感性。结果表明,所用引物对这两株病毒具有相同的特异性。这三种提取ＲＮＡ的敏感性差异不大,敏感性从高到低依次为：ＧＰ、ＭＢＰ和标准法,检测汉滩病毒的效价分别为０．０１、０．０１和０．１ＴＣＩＤ５０／２００μｌ;提取ＲＮＡ的时间长短分别为：标准法为２．５ｈ,ＧＰ为１ｈ,ＭＢＰ为０．５ｈ,可见ＭＢＰ用时最少。使用ＧＰ和ＭＢＰ提取ＲＮＡ时,可以在室温下进行,不需要低温离心。所以,ＭＢＰ最适合于实验室和临床病原体的快速检测,特别是对采集的环境样品的快速检测