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排序方式: 共有217条查询结果,搜索用时 31 毫秒
51.
Salinity controls soil microbial community structure and function in coastal estuarine wetlands 总被引:1,自引:0,他引:1
Guangliang Zhang Junhong Bai Christoph C. Tebbe Qingqing Zhao Jia Jia Wei Wang Xin Wang Lu Yu 《Environmental microbiology》2021,23(2):1020-1037
Soil salinity acts as a critical environmental filter on microbial communities, but the consequences for microbial diversity and biogeochemical processes are poorly understood. Here, we characterized soil bacterial communities and microbial functional genes in a coastal estuarine wetland ecosystem across a gradient (~5 km) ranging from oligohaline to hypersaline habitats by applying the PCR-amplified 16S rRNA (rRNA) genes sequencing and microarray-based GeoChip 5.0 respectively. Results showed that saline soils in marine intertidal and supratidal zone exhibited higher bacterial richness and Faith's phylogenetic diversity than that in the freshwater-affected habitats. The relative abundance of taxa assigned to Gammaproteobacteria, Bacteroidetes and Firmicutes was higher with increasing salinity, while those affiliated with Acidobacteria, Chloroflexi and Cyanobacteria were more prevalent in wetland soils with low salinity. The phylogenetic inferences demonstrated the deterministic role of salinity filtering on the bacterial community assembly processes. The abundance of most functional genes involved in carbon degradation and nitrogen cycling correlated negatively with salinity, except for the hzo gene, suggesting a critical role of the anammox process in tidal affected zones. Overall, the salinity filtering effect shapes the soil bacterial community composition, and soil salinity act as a critical inhibitor in the soil biogeochemical processes in estuary ecosystems. 相似文献
52.
Deyao Du Yu Zhu Junhong Wei Yuqing Tian Guoqing Niu Huarong Tan 《Applied microbiology and biotechnology》2013,97(14):6383-6396
Nikkomycins and gougerotin are peptidyl nucleoside antibiotics with broad biological activities. The nikkomycin biosynthetic gene cluster comprises one pathway-specific regulatory gene (sanG) and 21 structural genes, whereas the gene cluster for gougerotin biosynthesis includes one putative regulatory gene, one major facilitator superfamily transporter gene, and 13 structural genes. In the present study, we introduced sanG driven by six different promoters into Streptomyces ansochromogenes TH322. Nikkomycin production was increased significantly with the highest increase in engineered strain harboring hrdB promoter-driven sanG. In the meantime, we replaced the native promoter of key structural genes in the gougerotin (gou) gene cluster with the hrdB promoters. The heterologous producer Streptomyces coelicolor M1146 harboring the modified gene cluster produced gougerotin up to 10-fold more than strains carrying the unmodified cluster. Therefore, genetic manipulations of genes involved in antibiotics biosynthesis with the constitutive hrdB promoter present a robust, easy-to-use system generally useful for the improvement of antibiotics production in Streptomyces. 相似文献
53.
Two experiments were conducted to investigate the automatic processing of emotional facial expressions while performing low or high demand cognitive tasks under unattended conditions. In Experiment 1, 35 subjects performed low (judging the structure of Chinese words) and high (judging the tone of Chinese words) cognitive load tasks while exposed to unattended pictures of fearful, neutral, or happy faces. The results revealed that the reaction time was slower and the performance accuracy was higher while performing the low cognitive load task than while performing the high cognitive load task. Exposure to fearful faces resulted in significantly longer reaction times and lower accuracy than exposure to neutral faces on the low cognitive load task. In Experiment 2, 26 subjects performed the same word judgment tasks and their brain event-related potentials (ERPs) were measured for a period of 800 ms after the onset of the task stimulus. The amplitudes of the early component of ERP around 176 ms (P2) elicited by unattended fearful faces over frontal-central-parietal recording sites was significantly larger than those elicited by unattended neutral faces while performing the word structure judgment task. Together, the findings of the two experiments indicated that unattended fearful faces captured significantly more attention resources than unattended neutral faces on a low cognitive load task, but not on a high cognitive load task. It was concluded that fearful faces could automatically capture attention if residues of attention resources were available under the unattended condition. 相似文献
54.
55.
Expression of artificial microRNAs in tomato confers efficient and stable virus resistance in a cell-autonomous manner 总被引:1,自引:0,他引:1
Xiaohui Zhang Hanxia Li Junhong Zhang Chanjuan Zhang Pengjuan Gong Khurram Ziaf Fangming Xiao Zhibiao Ye 《Transgenic research》2011,20(3):569-581
Expression of artificial microRNAs (amiRNAs) in plants can target and degrade the invading viral RNA, consequently conferring
virus resistance. Two amiRNAs, targeting the coding sequence shared by the 2a and 2b genes and the highly conserved 3′ untranslated
region (UTR) of Cucumber mosaic virus (CMV), respectively, were generated and introduced into the susceptible tomato. The transgenic tomato plants expressing amiRNAs
displayed effective resistance to CMV infection and CMV mixed with non-targeted viruses, including tobacco mosaic virus and tomato yellow leaf curl virus. A series of grafting assays indicate scions originated from the transgenic tomato plant maintain stable resistance to CMV
infection after grafted onto a CMV-infected rootstock. However, the grafting assay also suggests that the amiRNA-mediated
resistance acts in a cell-autonomous manner and the amiRNA signal cannot be transmitted over long distances through the vascular
system. Moreover, transgenic plants expressing amiRNA targeting the 2a and 2b viral genes displayed slightly more effective
to repress CMV RNA accumulation than transgenic plants expressing amiRNA targeting the 3′ UTR of viral genome did. Our work
provides new evidence of the use of amiRNAs as an effective approach to engineer viral resistance in the tomato and possibly
in other crops. 相似文献
56.
Liu J Liu H Zhang Y Qiu L Su F Li F Su Z Li J 《Applied microbiology and biotechnology》2007,74(1):249-255
Soybean hull peroxidase (SHP) was crystallised from an enzyme solution with low purity by a simple method. The enzyme solution
was purified by cooperation salting out of acetone and ammonium sulphate, and lumpy crystals were obtained with the size of
about 40 × 30 μm when ammonium sulphate was quickly added to the enzyme solution. The crystal was examined and confirmed to
be an SHP crystal by the method of activity test. The result shows that, though the purity of the enzyme solution was not
high, crystals could be formed when the enzyme solution rapidly reached to a degree of supersaturation, which was different
from the traditional methods of protein crystallisation. Additionally, a purification method of acetone and ammonium sulphate
fractional salting out was also studied, in which the procedure was simplified, and a satisfactory purification effect was
obtained. 相似文献
57.
This study focused on the mitigation of membrane fouling caused by struvite precipitation using a dialyzer/zeolite (D/Z) unit in an anaerobic membrane bioreactor. The D/Z unit was designed to selectively remove NH(4)+ ions, one of the main components of the inorganic foulant, struvite. The maximum mass transfer coefficient for NH(4)+ through the dialyzer was estimated to be 0.92 l m(-2)h(-1), whereas the Na-substituted zeolite had the highest ion exchange capacity with respect to ammonium among intact or differently pretreated zeolites. During a single passage of dialysate through the zeolite column, substantial NH(4)+ removal (in excess of 90%) was achieved, leading to the reduction in struvite precipitation in the digester. The D/Z unit played a significant role in controlling struvite precipitation, thereby enhancing permeate flux for the case of the ceramic membrane, in which struvite fouling would be more pronounced compared to the polymeric membrane. For the polymeric membrane, however, no significant improvement in flux was observed even with the D/Z unit because the fouling of the polymeric membrane was mainly due to the deposition of biomass rather than the struvite precipitation. 相似文献
58.
Jie Ye Xin Wang Wenqian Wang Huiyang Yu Guo Ai Changxing Li Pengya Sun Xianyu Wang Hanxia Li Bo Ouyang Junhong Zhang Yuyang Zhang Heyou Han James J Giovannoni Zhangjun Fei Zhibiao Ye 《Plant physiology》2021,186(4):2078
Tomato (Solanum lycopersicum) is a highly valuable fruit crop, and yield is one of the most important agronomic traits. However, the genetic architecture underlying tomato yield-related traits has not been fully addressed. Based on ∼4.4 million single nucleotide polymorphisms obtained from 605 diverse accessions, we performed a comprehensive genome-wide association study for 27 agronomic traits in tomato. A total of 239 significant associations corresponding to 129 loci, harboring many previously reported and additional genes related to vegetative and reproductive development, were identified, and these loci explained an average of ∼8.8% of the phenotypic variance. A total of 51 loci associated with 25 traits have been under selection during tomato domestication and improvement. Furthermore, a candidate gene, Sl-ACTIVATED MALATE TRANSPORTER15, that encodes an aluminum-activated malate transporter was functionally characterized and shown to act as a pivotal regulator of leaf stomata formation, thereby affecting photosynthesis and drought resistance. This study provides valuable information for tomato genetic research and breeding.A large-scale genome-wide association study sheds light on genetic and genomic bases underlying important yield-related traits in tomato. 相似文献
59.
60.
Annexins have been suggested to play pivotal roles in stress resistance and plant development. However, related studies on fruit-bearing plants, especially on fruit development, are very limited. In the present study, we provide a comprehensive overview of the annexin family in tomato, describing the gene structure, promoter cis-regulatory elements, organ expression profile, and gene expression patterns under hormone and stress treatments. Bioinformatic analysis revealed that the nine tomato annexins were structurally different from their animal counterparts, but highly conserved annexin domains were still found in most of them. Cis-regulatory element prediction showed that there were important elements in the 2kb upstream promoter regions, including stress- and hormone-responsive-related elements. The expression patterns of these genes were investigated, and the results revealed that they were regulated under developmental processes and environmental stimuli. Among them, AnnSl1.1 and AnnSl2 were highly expressed in most of the tested organs. Genes preferentially or specifically expressed in organs, such as stigma or ovary (AnnSl6), stamen (AnnSl8), and fruit pericarp (AnnSl1.2 and AnnSl9), were identified. Some annexin genes were induced by plant hormones including abscisic acid (AnnSl3, AnnSl6, AnnSl8, and AnnSl9) and gibberellic acid (AnnSl1.1, AnnSl1.2, AnnSl4, and AnnSl7). Most of these annexin genes were induced by salt, drought, wounding, and heat or cold stresses. The present study provides significant information for understanding the diverse roles of annexins in tomato growth and development. 相似文献