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191.
Determination of substrate utilization patterns of soil microbial communities: An approach to assess population changes after hydrocarbon pollution 总被引:13,自引:0,他引:13
Abstract: Changes in hydrocarbon content in soils resulted in characteristic shifts of the substrate utilization patterns as tested with the Biolog system. The altered patterns of substrate utilization corresponded to similar changes in abundance of hydrocarbon-utilizing bacteria and the occurrence of specific bacterial groups in the soils. Substrate utilization patterns as recorded with the Biolog system are suitable for rapidly assessing dynamics of autochthonous soil communities and evaluating their biodegradative potential. 相似文献
192.
Iris Gruska Waltraut Jekabsons Wolfgang Schuster 《Molecular & general genetics : MGG》1995,247(5):529-536
We have characterized a mitochondrial gene in Oenothera, designated orf454, capable of encoding a component of the cytochrome c biogenesis system. This open reading frame is interrupted by an intron of 941 nucleotides showing high similarity to a group II intron residing in the rpl2 gene. RNA editing, which is observed at 18 cytidine positions within the orf454 reading frame, improves the similarity to protein-coding sequences in bacteria and higher plants and removes the last 16 amino acids. orf454 also shows high sequence similarity to two overlapping reading frames (orf169 and orf322) of Marchantia mitochondria. These ORFs belong to an operon-like cluster of genes in the liverwort that is not conserved in Oenothera mitochondria. However, in bacteria these reading frames are organized like the Marchantia gene cluster. It has been shown by genetical analysis in Rhodobacter capsulatus that these genes are essential for cytochrome c biogenesis. Genes of bacterial operons — ccl1 in Rhodobacter and yejR and nrfE in Escherichia coli — show high sequence similarity to the mitochondrial reading frames orf577 and orf454 of Oenothera. orf454, which we describe here, is homologous to the C-terminal region of these bacterial genes, while the previously described orf577 is homologous to the N-terminal region. 相似文献
193.
Abraham J. Koster Jochen Walz Andrei Lupas Wolfgang Baumeister 《Molecular biology reports》1995,21(1):11-20
The 26S proteasome is the central protease of the ubiquitin-dependent pathway of protein degradation. The molecule has a molecular mass of approximately 2000 kD and has a highly conserved structure in eukaryotes. The 26S proteasome is formed by a barrel-shaped 20S core complex and two polar 19S complexes. The 20S complex has C2 symmetry and is formed by four seven-membered rings of which the outer rings (-type subunits) are rotated by 25.7° relative to the inner rings while the inner rings (-type subunits) are in register. From a comparison of the activity and regulation of the 26S and 20S particles it can be deduced that the 20S particle contains the protease activity while the 19S complex contains isopeptidase, ATPase and protein unfolding activities. In this article we describe the structures of various proteasome complexes as determined by electron microscopy and discuss structural implications of their subunit sequences. 相似文献
194.
The Penicillium frequentans strain Bi 7/2, using phenol as a sole source of carbon and energy,transformed the fluorinated
phenols 2,3-, 2,4-, 2,5-and 3,4-difluorophenol rapidly. After growth on phenol, resting mycelia of the fungus converted the
difluorophenols completely at an initial concentration of 0.5 mM within 6 hours. The corresponding difluorinated catechols
were found to be intermediates of all difluorophenols investigated. A relatively unspecific phenol hydroxylase catalyzed this
hydroxylation step and showed activities towards all difluorophenols tested. One difluorocatechol was formed from each difluorophenol
substituted with fluorine in the ortho-position, whereas two catechols were formed from 3,4-difluorophenol, due to its two
vacant ortho-positions. A partial defluorination (50-77%) was observed in all cases.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
195.
196.
Roman Bo?a Peter Baran L'ubor Dlháň Hartmut Fuess Wolfgang Haase Franz Renz Wolfgang Linert Ingrid Svoboda Rüdiger Werner 《Inorganica chimica acta》1997,260(2):4119-136
The structure of the [Fe(bzimpy)2](ClO4)2·xH2O system (x = 0.25) was determined by single crystal X-ray structure analysis. The Fe(II) ion is hexacoordinated by six donor nitrogen atoms. The magnetic properties of the complex were investigated by powder magnetic susceptibility measurements and ESR. The freshly prepared sample does not show any traces of iron(III) impurities but these are formed as a function of time. After 1 year the sample contains 8.2% iron(III) as shown by UV spectroscopy and indicated by geff = 4.3 and 2.0 in its ESR spectrum. This explains the recorded ξ versus T behaviour at low temperature: with increasing temperature the ξ value decreases according to the Curie-Weiss law for a S = 5/2 system having an effective g = 4.3. Above 220 K a continuous increase in the ξ value is observed and a spin crossover applies. The spin transition is not complete at room temperature. A pronounced hysteresis is observed upon heating/cooling the sample between 220 and 414 K on the basis of magnetic data and infrared spectra. 相似文献
197.
Wolfgang Stephan 《Journal of mathematical biology》1995,34(1):95-109
A population genetic two-locus model with additive, directional selection and recombination is considered. It is assumed that recombination is weaker than selection; i.e., the recombination parameter r is smaller than the selection coefficients. This assumption is appropriate for describing the effects of two-locus selection at the molecular level. The model is formulated in terms of ordinary differential equations (ODES) for the gamete frequencies x = (x
1, x
2, x
3, x
4), defined on the simplex S
4. The ODEs are analyzed using first a regular pertubation technique. However, this approach yields satisfactory results only if r is very small relative to the selection coefficients and if the initial values x(0) are in the interior part of S
4. To cope with this problem, a novel two-scale perturbation method is proposed which rests on the theory of averaging of vectorfields. It is demonstrated that the zeroth-order solution of this two-scale approach approximates the numerical solution of the model well, even if recombination rate is on the order of the selection coefficients. 相似文献
198.
199.
Christoph Winterhalter Peter Heinrich Anton Candussio Günther Wich Wolfgang Liebl 《Molecular microbiology》1995,15(3):431-444
A segment of Thermotoga maritima strain MSB8 chromosomal DNA was isolated which encodes an endo-1,4-β-D-xylanase, and the nucleotide sequence of the xylanase gene, designated xynA, was determined. With a half-life of about 40 min at 90°C at the optimal pH of 6.2, purified recombinant XynA is one of the most thermostable xylanases known. XynA is a 1059-amino-acid (?120 kDa) modular enzyme composed of an N-terminal signal peptide and five domains, in the order A1-A2-B-C1-C2. By comparison with other xylanases of family 10 of glycosyl hydrolases, the central ?340-amino-acid part (domain B) of XynA represents the catalytic domain. The N terminal ?150-amino-acid repeated domains (A1-A2) have no significant similarity to the C-terminal ?170-amino-acid repeated domains (C1-C2). Cellulose-binding studies with truncated XynA derivatives and hybrid proteins indicated that the C-terminal repeated domains mediate the binding of XynA to microcrystalline cellulose and that C2 alone can also promote cellulose binding. C1 and C2 did not share amino acid sequence similarity with any other known cellulose-binding domain (CBD) and thus are CBDS of a novel type. Structurally related protein segments which are probably also CBDs were found in other multi-domain xylanolytic enzymes. Deletion of the N-terminal repeated domains or of all the non-catalytic domains resulted In substantially reduced tbermostability while a truncated xylanase derivative lacking the C-terminal tandem repeat was as thermostable as the full-length enzyme. It is argued that the multidomain organization of some enzymes may be one of the strategies adopted by thermophiles to protect their proteins against thermal denaturation. 相似文献
200.