全文获取类型
收费全文 | 10076篇 |
免费 | 773篇 |
国内免费 | 1篇 |
出版年
2023年 | 25篇 |
2022年 | 104篇 |
2021年 | 208篇 |
2020年 | 126篇 |
2019年 | 179篇 |
2018年 | 287篇 |
2017年 | 219篇 |
2016年 | 359篇 |
2015年 | 583篇 |
2014年 | 661篇 |
2013年 | 680篇 |
2012年 | 921篇 |
2011年 | 845篇 |
2010年 | 554篇 |
2009年 | 469篇 |
2008年 | 645篇 |
2007年 | 538篇 |
2006年 | 484篇 |
2005年 | 465篇 |
2004年 | 401篇 |
2003年 | 342篇 |
2002年 | 295篇 |
2001年 | 196篇 |
2000年 | 186篇 |
1999年 | 134篇 |
1998年 | 65篇 |
1997年 | 45篇 |
1996年 | 28篇 |
1995年 | 46篇 |
1994年 | 40篇 |
1993年 | 31篇 |
1992年 | 57篇 |
1991年 | 51篇 |
1990年 | 62篇 |
1989年 | 42篇 |
1988年 | 35篇 |
1987年 | 33篇 |
1986年 | 30篇 |
1985年 | 39篇 |
1984年 | 27篇 |
1983年 | 28篇 |
1982年 | 20篇 |
1981年 | 23篇 |
1979年 | 21篇 |
1975年 | 15篇 |
1974年 | 18篇 |
1973年 | 19篇 |
1971年 | 23篇 |
1970年 | 17篇 |
1968年 | 19篇 |
排序方式: 共有10000条查询结果,搜索用时 468 毫秒
181.
Jang Hye Jin Choi Ji Yeon Kim Kangjoon Yong Seung Hyun Kim Yeon Wook Kim Song Yee Kim Eun Young Jung Ji Ye Kang Young Ae Park Moo Suk Kim Young Sam Cho Young-Jae Lee Sang Hoon 《Respiratory research》2021,22(1):1-9
IL-35 subunit EBI3 is up-regulated in pulmonary fibrosis tissues. In this study, we investigated the pathological role of EBI3 in pulmonary fibrosis and dissected the underlying molecular mechanism. Bleomycin-induced pulmonary fibrosis mouse model was established, and samples were performed gene expression analyses through RNAseq, qRT-PCR and Western blot. Wild type and EBI3 knockout mice were exposed to bleomycin to investigate the pathological role of IL-35, via lung function and gene expression analyses. Primary lung epithelial cells were used to dissect the regulatory mechanism of EBI3 on STAT1/STAT4 and STAT3. IL-35 was elevated in both human and mouse with pulmonary fibrosis. EBI3 knockdown aggravated the symptoms of pulmonary fibrosis in mice. EBI3 deficiency enhanced the expressions of fibrotic and extracellular matrix-associated genes. Mechanistically, IL-35 activated STAT1 and STAT4, which in turn suppressed DNA enrichment of STAT3 and inhibited the fibrosis process. IL-35 might be one of the potential therapeutic targets for bleomycin-induced pulmonary fibrosis. 相似文献
182.
183.
Yulia Zilber Sima Babayeva Jung Hwa Seo Jia Jia Liu Steven Mootin Elena Torban 《Molecular biology of the cell》2013,24(5):555-565
The planar cell polarity (PCP) pathway controls multiple cellular processes during vertebrate development. Recently the PCP pathway was implicated in ciliogenesis and in ciliary function. The primary cilium is an apically projecting solitary organelle that is generated via polarized intracellular trafficking. Because it acts as a signaling nexus, defects in ciliogenesis or cilial function cause multiple congenital anomalies in vertebrates. Loss of the PCP effector Fuzzy affects PCP signaling and formation of primary cilia; however, the mechanisms underlying these processes are largely unknown. Here we report that Fuzzy localizes to the basal body and ciliary axoneme and is essential for ciliogenesis by delivering Rab8 to the basal body and primary cilium. Fuzzy appears to control subcellular localization of the core PCP protein Dishevelled, recruiting it to Rab8-positive vesicles and to the basal body and cilium. We show that loss of Fuzzy results in inhibition of PCP signaling and hyperactivation of the canonical WNT pathway. We propose a mechanism by which Fuzzy participates in ciliogenesis and affects both canonical WNT and PCP signaling. 相似文献
184.
185.
Maki Ohtani Jun-ichi Hikima Tae-Sung Jung Hidehiro Kondo Ikuo Hirono Haruko Takeyama Takashi Aoki 《Fish & shellfish immunology》2013,34(2):724-728
Phage display libraries are used to screen for nucleotide sequences that encode immunoglobulin variable (V) regions that are specific for a target antigen. We previously constructed an immunoglobulin new antigen receptor (IgNAR) phage display library. Here we used this library to obtain an IgNAR V region that is specific for viral hemorrhagic septicemia virus (VHSV). A phage clone (clone 653) was found to be specific for VHSV by the biopanning method. The V region of clone 653 was used to construct a 6 × His tagged recombinant IgNAR-653 V protein (rIgNAR-653) using the Escherichia coli pET system. The rIgNAR-653 protein bound specifically to VHSV, confirming its activity. 相似文献
186.
187.
188.
Seung Ho Jung Arpit Saxena Kamaljeet Kaur Emma Fletcher Venkatesh Ponemone James M. Nottingham Joseph A. Sheppe Maria Petroni Jennifer Greene Kelly Graves Manjeshwar Shrinath Baliga Raja Fayad 《Cytokine》2013,61(2):459-468
Inflammatory bowel diseases (IBDs) are chronic inflammatory disorders of the gastrointestinal tract that affect more than 3 million people worldwide, but the pathological etiology is still unknown. The overall purpose of our investigations was to elucidate the possibility of pathological causes of IBD, and therefore, we determined the difference of inflammatory cytokine profiles in adipose tissue macrophages (ATMs) and T lymphocytes (ATTs) obtained near active lesions of IBD; investigated whether the alteration in ATM activation induces genes involved in collagen formation; and evaluated the effects of fatty acid oxidation inhibitors on factors involved in inflammation and collagen production by ATMs in IBD. Adipose tissues (ATs) were collected near active lesions and also at the margin of resected segments of the bowel from IBD patients with ulcerative colitis (UC) and CD (n = 14/group). Normal appearing ATs from control subjects (n = 14) who had colon resection for adenocarcinoma were collected as far away from the cancer lesion as possible to rule out possible changes. Compared with inactive disease lesions, ATMs and ATTs from active lesions released more IL-6, IL-4 and IL-13. Treatments of cytokine IL-4 and/or IL-13 to ATMs reduced iNOS expression but increased Arg-I expression which were exacerbated when treated with T cell- and adipocyte-conditioned medium. However, fatty acid oxidation inhibitors prevented the effects of cytokines IL-4 and/or IL-13 on iNOS and Arg-I expressions. This study was the first to show the effect of IL-4 and IL-13 on collagen formation, through iNOS and Arg-I expressions, that was exacerbated in a condition that mimics in vivo condition of active lesions. Moreover, our study was the first to provide potential benefits of fatty acid oxidation inhibitors to ATMs on preventing collagen formation; thus, providing therapeutic implications for individuals with intestinal fibrosis and stricture lesions, although future study should be guaranteed to elucidate the underlying mechanisms. 相似文献
189.
Hee Kyoung Kang Jung Han Suh Jung Jin Lee Sun Hee Yoon Jin Won Hyun Seong Won Choi 《Free radical research》2013,47(7):773-779
The present study was undertaken to examine the effect of l-ascorbic acid (LAA) on the growth of HL-60 promyelocytic leukemia cells, besides induction of apoptosis. LAA (≥10-4?M) was found to markedly inhibit the proliferation of HL-60 in liquid culture and clonogenicity in semisolid culture. Moreover, LAA-treated HL-60 showed activity to produce chemiluminescence and expressed CD 66b cell surface antigens, indicating that LAA induces the differentiation of HL-60 mainly into granulocytes. The results are supported by morphological changes of LAA-treated HL-60 into segmented neutrophils. Therefore, the inhibitory effect of LAA on the growth of HL-60 cells seems to arise from the induction of differentiation. To assess the potential role of LAA, cells were exposed to oxygen radical scavengers in the absence or presence of LAA. Catalase abolished and superoxide dismutase promoted LAA-induced differentiation of HL-60. Thus, H2O2 produced as a result of LAA treatment seems to play a major role in induction of HL-60 differentiation. 相似文献