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The formation of R-prime plasmids was selected in crosses involving soybean microsymbionts with genomic Tn5 insertions and carrying plasmid pJB3JI (with one IS2) copy as donors and Escherichia coli HB101 as recipient. Whereas the parent plasmid was 60 kb, recombinant plasmids between 76 kb and 121 kb were obtained. Restriction and Southern analyses confirmed the mobilization of Tn5 on four R-primes from Bradyrhizobium japonicum I-110 and on an R-prime plasmid from Rhizobium fredii HH303. The largest R-prime plasmid was obtained from the rescue of two symbiotically defective R. fredii mutant strains that required adenosine.Non-standard abbreviation TDP
transposon donor pool
Scientific article number A-4728 and contribution number 7724 of the Maryland Agricultural Experiment Station 相似文献
65.
Dennis W. Jung Gerald P. Brierley 《Biochemical and biophysical research communications》1982,106(4):1372-1377
Heart mitochondria respiring in the presence of Pi release endogenous K+ to a sucrose medium when an uncoupler is added. The uncoupled mitochondria retain K+, however, if the oxidation of NAD(P)H is prevented by the addition of rotenone or antimycin. Addition of rotenone, once the uncoupler-dependent K+-efflux has been initiated, results in a rapid reduction of NAD(P) and a simultaneous decrease in permeability to K+. These changes are independent of respiration. The results suggest that a latent pathway for K+-permeability is present in the membrane, that it can be opened and closed reversibly, and that it reflects, either directly or indirectly, the redox status of mitochondrial pyridine nucleotides. The possible relationship of this putative pathway to those available for Ca2+ uptake and release is considered. 相似文献
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Siddhartha Kumar Bhaumik Manoj Kumar Singh Subir Karmakar Tripti De 《Glycoconjugate journal》2009,26(6):663-673
As compared to cutaneous leishmaniasis, vaccination against visceral leishmaniasis (VL) has received limited attention. In
this study, we demonstrate for the first time that an UDP-Galactose: N-acetylglucosamine β 1–4 galactosyltransferase (GenBank Accession No. EF159943) expressing attenuated LD clonal population (A-LD) is able to confer protection against the experimental challenge with the virulent LD AG83 parasite. A-LD was also effective in established leishmania infection. The vaccinated animals showed both cell mediated
(in vitro T-cell proliferation, and DTH response) and humoral responses (Th1 type). These results demonstrate the potential of the
attenuated clones as an immunotherapeutic and immunoprophylactic agent against visceral leishmaniasis. 相似文献
68.
Energy expenditure was measured during pregnancy in seven primigravid women at 12-15, 25-28, and 34-36 weeks and after the cessation of lactation. On each occasion the resting metabolic rate and the increase in metabolic rate after ingestion of a liquid test meal were measured by indirect calorimetry. In absolute terms the resting metabolic rate increased steadily during pregnancy but when expressed per unit of body weight no change was found. The energetic response to a mixed constituent meal was significantly reduced by 28% in the middle trimester of pregnancy. These findings suggest a possible maternal adaptation to increase energetic efficiency at a time when the energy demands of the fetus are high. 相似文献
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Shallow-water vegetated estuarine habitats, notably seagrass, mangrove and saltmarsh, are known to be important habitats for
many species of small or juvenile fish in temperate Australia. However, the movement of fish between these habitats is poorly
understood, and yet critical to the management of the estuarine fisheries resource. We installed a series of buoyant pop nets
in adjacent stands of seagrass, mangrove and saltmarsh in order to determine how relative abundance of fishes varied through
lunar cycles. Nets were released in all habitats at the peak of the monthly spring tide for 12 months, and in the seagrass
habitat at the peak of the neap tide also. The assemblage of fish in each habitat differed during the spring tides. The seagrass
assemblage differed between spring and neap tide, with the neap tide assemblage showing greater abundances of fish, particularly
those species which visited the adjacent habitats when inundated during spring tides. The result supports the hypothesis that
fish move from the seagrass to the adjacent mangrove and saltmarsh during spring tides, taking advantage of high abundances
of zooplankton, and use seagrass as a refuge during lower tides. The restoration and preservation of mangrove and saltmarsh
utility as fish habitat may in some situations be linked to the proximity of available seagrass. 相似文献