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41.
报道了测定CK-MM亚型的聚焦色谱法,此法简单,快速,结果可靠,线性范围宽,最低检测限(8U/L)较正常参考值低,比国外报道的类似方法高6倍以上,分离度亦有改进.测定了20例健康人血清亚型分布,与文献报道结果相近.该法自动化程度高,已在急性心梗的诊断中实际应用. 相似文献
42.
质体醌重组的D1/D2/Cytb559复合物的荧光衰减动力学和光破坏作用 总被引:3,自引:0,他引:3
光系统Ⅱ反应中心D1/D2/Cytb559 在分离纯化过程中失去了电子受体QA 和QB,人工合成的质体醌可以与D1/D2/Cytb559 复合物发生重组。癸基质体醌(DPQ)与D1/D2/Cytb599 复合物的重组导致该复合物的荧光强度下降及发射光谱蓝移,同时两个与光化学活性相关的长寿命(24 ns和73 ns)荧光衰减组分占整个荧光的百分数下降,这些结果表明DPQ作为Pheo- 的电子受体,限制了P680+ ·Pheo- 的电荷重组。DPQ 的加入对D1/D2/Cytb559复合物中Chla 分子的光破坏敏感性影响不大,但β-胡萝卜素在加入DPQ 之后可以被光照破坏,这个过程可能与β-胡萝卜素的生理功能相关。 相似文献
43.
氯化镧对玉米根切段钾离子外渗影响的动力学研究 总被引:9,自引:0,他引:9
研究了氯化镧对玉米(Zea mays L.)“京早8号”根切段细胞膜透性及质子分泌的影响,并用动力学方法研究了钾离子外渗过程的变化。氯化镧处理可降低外渗液的电导率及K~ 和糖的外渗量,使质子分泌活动增强。用动力学方法分析玉米根切段K~ 外渗过程的结果表明:(1)应用于K~ 吸收研究的数学模型也能适应于K~ 外渗的研究;(2)在LaCl_3和(或)CaCl_2存在的条件下,最大吸收速度(V_(max))升高,而米氏常数(K_m)没有变化;(3)在LaCl_3和(或)CaCl_2存在的条件下,K~ 外渗量降低是由于最大吸收速度(V_(max))升高所致。 相似文献
44.
Xu Yuping Wang Lu Tang Quehui Naselli-Flores Luigi Jeppesen Erik Han Bo-Ping 《Ecosystems》2023,26(4):752-767
Ecosystems - The relationship between species diversity and ecosystem functioning is one of central topics in modern ecology, but variable and controversial patterns have been found depending on... 相似文献
45.
Nan Tang Kai Zhu Cheng Jiang Zhiyong Xiong Qiangping Wang Junjun Li Weiming Xu 《Journal of cellular and molecular medicine》2023,27(2):277-286
RNF7 has been reported to play critical roles in various cancers. However, the underlying mechanisms of RNF7 in glioma development remain largely unknown. Herein, the expression level of RNF7 was examined in tissues by quantitative real-time PCR, Western blotting and immunohistochemistry. The effect of RNF7 on glioma progression was measured by performing CCK-8 and apoptosis assays, cell cycle-related experiments and animal experiments. The effect of RNF7 on PI3K/AKT signalling pathway was tested by Western blotting. First, we found that RNF7 was upregulated in tumour tissue compared with normal brain tissue, especially in high-grade glioma, and the high expression of RNF7 was significantly related to tumour size, Karnofsky Performance Scale score and a poor prognosis. Second, RNF7 overexpression facilitated tumour cell cycle progression and cell proliferation and suppressed apoptosis. Conversely, RNF7 knockdown suppressed tumour cell cycle progression and cell proliferation and facilitated apoptosis. Furthermore, follow-up mechanistic studies indicated that RNF7 could facilitate glioma cell proliferation and cell cycle progression and inhibit apoptosis by activating the PI3K/AKT signalling pathway. This study shows that RNF7 can clearly promote glioma cell proliferation by facilitating cell cycle progression and inhibiting apoptosis by activating the PI3K/AKT signalling pathway. Targeting the RNF7/PI3K/AKT axis may provide a new perspective on the prevention or treatment of glioma. 相似文献
46.
47.
Xiong Zhang Ming-You Peng En-Ming Feng Qing-Dan Li Lu Chen Hu-Cheng Yang Bing Guo Hong Liang Ying-Tong Di Lei Tang Ying Yan 《化学与生物多样性》2023,20(10):e202301061
Three previously undescribed diterpenoids, helioscopnoids A–C, and eight known compounds were isolated from the whole plants of Euphorbia helioscopia. Their structures were established by extensive analysis of spectra and data comparison with previous literatures. Among them, compound 4 was identified as 24,24-dimethoxy-25,26,27-trinoreuphan-3β-ol with revised configurations of C-13, C-14, and C-17 (13R*, 14R*, 17R*). Cytotoxicity assays revealed that all compounds exhibited varying levels of cytotoxicity against H1975 cells, with compound 9 displaying the most potent activity, as indicated by cell viability rates of 18.13 % and 20.76 % at concentrations of 20 μM and 5 μM, respectively. This study expands the understanding of E. helioscopia terpenoids’ structural diversity and biological activities, contributing to the exploration of potential therapeutic applications. 相似文献
48.
Han Tian Jin-Lin Yang Yirui Deng Wenhao Tang Ruiping Liu Chenyang Xu Peng Han Hong Jin Fan 《Liver Transplantation》2023,13(1):2202603
The progress of aqueous zinc batteries (AZBs) is limited by the poor cycling life due to Zn anode instability, including dendrite growth, surface corrosion, and passivation. Inspired by the anti-corrosion strategy of steel industry, a compounding corrosion inhibitor (CCI) is employed as the electrolyte additive for Zn metal anode protection. It is shown that CCI can spontaneously generate a uniform and ≈30 nm thick solid-electrolyte interphase (SEI) layer on Zn anode with a strong adhesion via Zn O bonding. This SEI layer efficiently prohibits water corrosion and guides homogeneous Zn deposition without obvious dendrite formation. This enables reversible Zn deposition and dissolution for over 1100 h under the condition of 1 mA cm−2 and 1 mAh cm−2 in symmetric cells. The Zn-MnO2 full cells with CCI-modified electrolyte deliver an ultralow capacity decay rate (0.013% per cycle) at 0.5 A g−1 over 1000 cycles. Such an innovative strategy paves a low-cost way to achieve AZBs with long lifespan. 相似文献
49.
Jinping Wei Xiaodong Wang Zeyu Hu Xiaojie Wang Jialiu Wang Jianfeng Wang Xueling Huang Zhensheng Kang Chunlei Tang 《植物学报(英文版)》2023,65(1):249-264
The obligate biotrophic fungus Puccinia striiformis f. sp. tritici (Pst) employs virulence effectors to disturb host immunity and causes devastating stripe rust disease. However, our understanding of how Pst effectors regulate host defense responses remains limited. In this study, we determined that the Pst effector Hasp98, which is highly expressed in Pst haustoria, inhibits plant immune responses triggered by flg22 or nonpathogenic bacteria. Overexpression of Hasp98 in wheat (Triticum aestivum) suppressed avirulent Pst-triggered immunity, leading to decreased H2O2 accumulation and promoting P. striiformis infection, whereas stable silencing of Hasp98 impaired P. striiformis pathogenicity. Hasp98 interacts with the wheat mitogen-activated protein kinase TaMAPK4, a positive regulator of plant resistance to stripe rust. The conserved TEY motif of TaMAPK4 is important for its kinase activity, which is required for the resistance function. We demonstrate that Hasp98 inhibits the kinase activity of TaMAPK4 and that the stable silencing of TaMAPK4 compromises wheat resistance against P. striiformis. These results suggest that Hasp98 acts as a virulence effector to interfere with the MAPK signaling pathway in wheat, thereby promoting P. striiformis infection. 相似文献
50.
Mu-jin Tang Shao-ling Zeng Jian-wu Chen Yong-xia Shi Wei Xu Mei-jin Yuan Yi Pang 《Insect Science》2003,10(4):221-229
A series of plasmids were constructed to examine the effects of p19 and orf1‐orf2 genes from Bacillus thuringiensis on Cyt1Aa synthesis and inclusion formation. The plasmids expressed the cyt1Aa gene along with either p19 or orf1‐orf2, or each of them coordinatively with p20 in the acrystalliferous strain of B. thuringiensis subsp. israelensis 4Q7. No effect on the expression of Cyt1Aa protein was found when P19 or Orf1‐Orf2 co‐expressed with Cyt1Aa. However, when including p20 gene, the constructs with p19 or orf1‐orf2 gene produced lower yield of Cyt1Aa proteins than without p19 or orf1‐orf2 gene. Electron microscopy observation and bioassay showed that P19 and Orf1‐Orf2 have no influence on the crystal size and toxicity of Cyt1Aa protein. It is presumed that P19 and Orf1‐Orf2 might have negative effects on Cyt1Aa synthesis in B. thuringiensis. 相似文献