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971.
We investigated changes in the sub-cellular distribution of glycelaldehyde-3-phosphate dehydrogenase (GAPDH) after X-ray irradiation in HeLa cells. Twenty-four h after irradiation at 5 Gy, nuclear GAPDH levels increased 2.6-fold, whereas total GAPDH levels increased only 1.2-fold. Knockdown of GAPDH using specific small interfering RNA (siRNA) led to sensitization to X-ray-induced cell death. These results suggest that GAPDH plays a role in the radioresponse. 相似文献
972.
Photometric microplate assay was performed for testing of paraoxon-inhibited acetylcholinesterase (AChE) using three reactivators for reactivation purposes: obidoxime, pralidoxime, and HI-6. 3-D graphs (percent of reactivation vs. concentration of reactivator and vs. time of reactivator effecting) were constructed for each reactivator to compare their efficacy. The best results were obtained using obidoxime where reactivation was near to 80%. Suitability of photometric microplates for following of reactivation procedures is discussed. 相似文献
973.
974.
零价铁(Fe0)具有高效还原转化多种污染物的能力,但不能实现污染物的矿化作用。微生物与Fe0的协同作用过程,以微生物为主导,Fe0起促进作用,可有效提高多种污染物的降解效率,实现污染物的彻底脱毒与无害化,因此利用微生物协同Fe0氧化进行环境修复具有广阔的应用前景。本文从微生物协同Fe0氧化的作用机理、菌种多样性及其在环境修复中的应用等研究进展进行综述,提出微生物协同Fe0氧化的环境修复研究中存在的主要问题和重点研究方向,以期在更全面、深入地认识这一过程的基础上,充分发挥其在环境修复中的作用。 相似文献
975.
Xiaojun Lu Xingbo Song Yuanxin Ye Xianzhong Liu Yi Zhou Lei Zhang Jun Wang Binwu Ying Lanlan Wang 《Molecular biology reports》2011,38(5):3101-3105
The BCR–ABL fusion gene in chromosome translocation, t (9; 22), and its product, p210BCR/ABL oncogenic tyrosine kinase, is
the underlying molecular mechanism that leads to the development of CML. Quantitative detection of BCR–ABL fusion gene has
become a reliable approach to diagnose and monitor CML. The aim of this study was to evaluate a Roche t (9; 22) kit in CML
diagnosis, monitoring treatment responses, and identification of relapse. Using BCR–ABL fusion gene-expressing K562 cells,
a series of standard samples were prepared and used to establish a curve for the calculation of BCR–ABL fusion gene expression
in patient samples. Our results indicate that PCR detection system with aforementioned kit has good reproducibility. In addition,
the relative concentration of BCR–ABL measured by PCR was in agreement with the patient’s response to the Imatinib treatment
and bone marrow morphology remission. Furthermore, we found that the relative concentration of BCR–ABL fusion gene increased
1–3 months before CML relapse was clinically and cytogenetically diagnosed, suggesting that the PCR-based BCR–ABL fusion gene
detection with t (9; 22) kit is able to diagnose the recurrence of CML at least 1 month earlier than the classic cytogenetic
analysis. In conclusion, detection of BCR–ABL fusion gene expression in CML using Roche t (9; 22) kit has great clinical value
in the primary diagnosis, monitoring treatment responses, and identification of relapse in CML patients. 相似文献
976.
977.
978.
The extensive use of phenol compounds and the inability to remove these compounds during wastewater treatment have resulted in the widespread occurrence of phenols in the natural environment. Phenols have been linked to serious risks to human and environmental health. Hence, the need to develop technologies that can effectively remove phenols from wastewater and source waters is a pressing challenge. In this study, light ceramic particles were immersed in activated sludge acclimated to degrade phenol, and microorganisms were allowed to attach to the particles surface to form biofilm. Then the ceramic particles with biofilm were moved into the photolytic circulating-bed biofilm reactor made of quartz glass, which was used for the degradation of phenol by three protocols: photolysis with UV light alone (P), biodegradation alone (B), and the two mechanisms operating simultaneously (photobiodegradation, P&B). The experimental results indicated that phenol removal rate was quickest by B experiment. However, P&B experiment gave more complete mineralization of phenol than that by other protocols. During P&B experiment, the microorganisms grown on porous ceramic carrier still kept the bioactivity degrading phenol, even under UV light irradiation. However, the dominant members of the bacterial community changed dramatically after the intimately coupled photobiodegradation, according to molecular biological analysis to the biofilm. Whereas Beijerinckia sp. was the dominant strain in the inoculum, it was replaced by Thauera sp. MZ1T that played a main role on degrading phenol during P&B experiment. 相似文献
979.
Pan Y Yang X Duan J Lu N Leung AS Tran V Hu Y Wu N Liu D Wang Z Yu X Chen C Zhang Y Wan K Liu J Zhu B 《Journal of bacteriology》2011,193(12):3152-3153
Mycobacterium bovis Bacille Calmette-Guérin (BCG) is the only vaccine available against tuberculosis (TB). A number of BCG strains are in use, and they exhibit biochemical and genetic differences. We report the genome sequences of four BCG strains representing different lineages, which will help to design more effective TB vaccines. 相似文献
980.
Emergy evaluation of the impact of waste exchanges on the sustainability of industrial systems 总被引:1,自引:0,他引:1
XiaoHong Zhang ShiHuai DengYanZong Zhang Gang YangLi Li Hui QiHong Xiao Jun WuYingJun Wang Fei Shen 《Ecological Engineering》2011,37(2):206-216
Emergy evaluation provided global performance indicators that were used to assess the impact of waste exchanges on the sustainability of a sulfuric acid production system (SAPS) and a titanium dioxide production system (TDPS) in Pan-zhi-hua City, China. Impact of emissions was quantified in terms of emergy and integrated into the classic emergy-based indicators. Results show that waste exchanges improve the sustainability of the two systems, although the SAPS benefits more from the waste exchanges than the TDPS from an economic perspective however, the impact of emissions reduces their sustainability to some degree. This study creates awareness and brings new insight to the gains achieved with resource recovery from waste in the interrelated industrial systems, which is derived from the mutualism relationship existing in natural ecosystems. Finally, this paper puts forward some corresponding suggestions. 相似文献