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61.
Julien R. C. Bergeron Liam J. Worrall Nikolaos G. Sgourakis Frank DiMaio Richard A. Pfuetzner Heather B. Felise Marija Vuckovic Angel C. Yu Samuel I. Miller David Baker Natalie C. J. Strynadka 《PLoS pathogens》2013,9(4)
The T3SS injectisome is a syringe-shaped macromolecular assembly found in pathogenic Gram-negative bacteria that allows for the direct delivery of virulence effectors into host cells. It is composed of a “basal body”, a lock-nut structure spanning both bacterial membranes, and a “needle” that protrudes away from the bacterial surface. A hollow channel spans throughout the apparatus, permitting the translocation of effector proteins from the bacterial cytosol to the host plasma membrane. The basal body is composed largely of three membrane-embedded proteins that form oligomerized concentric rings. Here, we report the crystal structures of three domains of the prototypical Salmonella SPI-1 basal body, and use a new approach incorporating symmetric flexible backbone docking and EM data to produce a model for their oligomeric assembly. The obtained models, validated by biochemical and in vivo assays, reveal the molecular details of the interactions driving basal body assembly, and notably demonstrate a conserved oligomerization mechanism. 相似文献
62.
Hélène Follet Delphine Farlay Yohann Bala Stéphanie Viguet-Carrin Evelyne Gineyts Brigitte Burt-Pichat Julien Wegrzyn Pierre Delmas Georges Boivin Roland Chapurlat 《PloS one》2013,8(2)
Previous studies have shown that microdamage accumulates in bone as a result of physiological loading and occurs naturally in human trabecular bone. The purpose of this study was to determine the factors associated with pre-existing microdamage in human vertebral trabecular bone, namely age, architecture, hardness, mineral and organic matrix. Trabecular bone cores were collected from human L2 vertebrae (n = 53) from donors 54–95 years of age (22 men and 30 women, 1 unknown) and previous cited parameters were evaluated. Collagen cross-link content (PYD, DPD, PEN and % of collagen) was measured on surrounding trabecular bone. We found that determinants of microdamage were mostly the age of donors, architecture, mineral characteristics and mature enzymatic cross-links. Moreover, linear microcracks were mostly associated with the bone matrix characteristics whereas diffuse damage was associated with architecture. We conclude that linear and diffuse types of microdamage seemed to have different determinants, with age being critical for both types. 相似文献
63.
Aleksandar Zocevic Fabien Vorimore Nadia Vicari Julien Gasparini Lisa Jacquin Konrad Sachse Simone Magnino Karine Laroucau 《PloS one》2013,8(3)
Recent evidence of the occurrence of atypical Chlamydiaceae strains in pigeons, different from the established Chlamydiaceae, requires the development of a specific and rapid detection tool to investigate their prevalence and significance. Here is described a new real-time PCR assay that allows specific detection of atypical Chlamydiaceae from pigeons. The assay has been used to assess the dissemination of these strains in field samples collected from Parisian pigeon populations in 2009. The results suggest a limited dissemination compared to the usually higher prevalence of Chlamydia psittaci that is the main species associated with avian chlamydiosis. 相似文献
64.
Blanche Saint-Béat Christine Dupuy Pierrick Bocher Julien Chalumeau Margot De Crignis Camille Fontaine Katell Guizien Johann Lavaud Sébastien Lefebvre Hélène Montanié Jean-Luc Mouget Francis Orvain Pierre-Yves Pascal Gwena?l Quaintenne Gilles Radenac Pierre Richard Frédéric Robin Alain F. Vézina Nathalie Niquil 《PloS one》2013,8(10)
The migratory shorebirds of the East Atlantic flyway land in huge numbers during a migratory stopover or wintering on the French Atlantic coast. The Brouage bare mudflat (Marennes-Oléron Bay, NE Atlantic) is one of the major stopover sites in France. The particular structure and function of a food web affects the efficiency of carbon transfer. The structure and functioning of the Brouage food web is crucial for the conservation of species landing within this area because it provides sufficient food, which allows shorebirds to reach the north of Europe where they nest. The aim of this study was to describe and understand which food web characteristics support nutritional needs of birds. Two food-web models were constructed, based on in situ measurements that were made in February 2008 (the presence of birds) and July 2008 (absence of birds). To complete the models, allometric relationships and additional data from the literature were used. The missing flow values of the food web models were estimated by Monte Carlo Markov Chain – Linear Inverse Modelling. The flow solutions obtained were used to calculate the ecological network analysis indices, which estimate the emergent properties of the functioning of a food-web.The total activities of the Brouage ecosystem in February and July are significantly different. The specialisation of the trophic links within the ecosystem does not appear to differ between the two models. In spite of a large export of carbon from the primary producer and detritus in winter, the higher recycling leads to a similar retention of carbon for the two seasons. It can be concluded that in February, the higher activity of the ecosystem coupled with a higher cycling and a mean internal organization, ensure the sufficient feeding of the migratory shorebirds. 相似文献
65.
Mia Svensk Sabrina Coste Bastien Gérard Eva Gril Frédéric Julien Pascale Maillard Clément Stahl Céline Leroy 《Physiologia plantarum》2020,170(4):488-507
Studying the response to drought stress of keystone epiphytes such as tank bromeliads is essential to better understand their resistance capacity to future climate change. The objective was to test whether there is any variation in the carbon, water and nutrient status among different leaf ontogenetic stages in a bromeliad rosette subjected to a gradient of drought stress. We used a semi-controlled experiment consisting in a gradient of water shortage in Aechmea aquilega and Lutheria splendens. For each bromeliad and drought treatment, three leaves were collected based on their position in the rosette and several functional traits related to water and nutrient status, and carbon metabolism were measured. We found that water status traits (relative water content, leaf succulence, osmotic and midday water potentials) and carbon metabolism traits (carbon assimilation, maximum quantum yield of photosystem II, chlorophyll and starch contents) decreased with increasing drought stress, while leaf soluble sugars and carbon, nitrogen and phosphorus contents remained unchanged. The different leaf ontogenetic stages showed only marginal variations when subjected to a gradient of drought. Resources were not reallocated between different leaf ontogenetic stages but we found a reallocation of soluble sugars from leaf starch reserves to the root system. Both species were capable of metabolic and physiological adjustments in response to drought. Overall, this study advances our understanding of the resistance of bromeliads faced with increasing drought stress and paves the way for in-depth reflection on their strategies to cope with water shortage. 相似文献
66.
Christian T?tzke Tatiana Miranda Wilfried Konrad Julien Gout Nikolay Kardjilov Martin Dawson Ingo Manke Anita Roth-Nebelsick 《Annals of botany》2013,111(4):723-730
Background and Aims
Cold neutron radiography was applied to directly observe embolism in conduits of liana stems with the aim to evaluate the suitability of this method for studying embolism formation and repair. Potential advantages of this method are a principally non-invasive imaging approach with low energy dose compared with synchrotron X-ray radiation, a good spatial and temporal resolution, and the possibility to observe the entire volume of stem portions with a length of several centimetres at one time.Methods
Complete and cut stems of Adenia lobata, Aristolochia macrophylla and Parthenocissus tricuspidata were radiographed at the neutron imaging facility CONRAD at the Helmholtz-Zentrum Berlin für Materialien und Energie, with each measurement cycle lasting several hours. Low attenuation gas spaces were separated from the high attenuation (water-containing) plant tissue using image processing.Key results
Severe cuts into the stem were necessary to induce embolism. The formation and temporal course of an embolism event could then be successfully observed in individual conduits. It was found that complete emptying of a vessel with a diameter of 100 µm required a time interval of 4 min. Furthermore, dehydration of the whole stem section could be monitored via decreasing attenuation of the neutrons.Conclusions
The results suggest that cold neutron radiography represents a useful tool for studying water relations in plant stems that has the potential to complement other non-invasive methods. 相似文献67.
Maxime Séleck Jean-Philippe Bizoux Gilles Colinet Michel-Pierre Faucon Arielle Guillaume Pierre Meerts Julien Piqueray Grégory Mahy 《Plant and Soil》2013,373(1-2):455-469
Aims
Define the chemical factors structuring plant communities of three copper-cobalt outcrops (Tenke-Fungurume, Katangan Copperbelt, D. R. Congo) presenting extreme gradients.Methods
To discriminate plant communities, 172 vegetation records of all species percentage cover were classified based on NMDS and the Calinski criterion. Soil samples were analyzed for 13 chemical factors and means compared among communities with ANOVA. Partial canonical correspondence analysis (pCCA) was used to determine amount of variation explained individually by each factor and site effect.Results
Seven communities were identified. Six of the studied communities were related to distinct sites. Site effect (6.0 % of global inertia) was identified as the most important factor related to plant communities’ variation followed by Cu (5.5 %), pH (3.6 %) and Co (3.5 %). Unique contribution of site effect (3.8 %) was higher than that of Cu (1.1 %) and Co (1.0 %).Conclusions
In restoration, not only Cu and Co contents will be important to maintain vegetation diversity, attention should also be given to co-varying factors potentially limiting toxicity of metals: pH, organic matter, Ca and Mn. Physical parameters were also identified as important in the creation of adequate conditions for diverse communities. Further studies should focus on the effect of physical parameters and geology. 相似文献68.
To preserve biodiversity and ecosystem functions in a globally changing world it is crucial to understand the effect of land use on ecosystem processes such as pollination. Floral colouration is known to be central in plant-pollinator interactions. To date, it is still unknown whether land use affects the colouration of flowering plant communities. To assess the effect of land use on the diversity and composition of flower colours in temperate grasslands, we collected data on the number of flowering plant species, blossom cover and flower reflectance spectra from 69 plant communities in two German regions, Schwäbische Alb (SA) and Hainich-Dün (HD). We analysed reflectance data of flower colours as they are perceived by honeybees and studied floral colour diversity based upon spectral loci of each flowering plant species in the Maxwell triangle. Before the first mowing, flower colour diversity decreased with increasing land-use intensity in SA, accompanied by a shift of mean flower colours of communities towards an increasing proportion of white blossom cover in both regions. By changing colour characteristics of grasslands, we suggest that increasing land-use intensity can affect the flower visitor fauna in terms of visitor behaviour and diversity. These changes may in turn influence plant reproduction in grassland plant communities. Our results indicate that land use is likely to affect communication processes between plants and flower visitors by altering flower colour traits. 相似文献
69.
Michaela Ecker Hervé Bocherens Marie-Anne Julien Florent Rivals Jean-Paul Raynal Marie-Hélène Moncel 《Journal of human evolution》2013
The Middle Palaeolithic site of Payre in southeastern France yields abundant archaeological material associated with fossil hominid remains. With its long sequence of Middle Pleistocene deposits, Payre is a key site to study the Middle Palaeolithic chronology of this region. This study is the first to investigate carbon and oxygen isotope contents of Neanderthal tooth enamel bioapatite, together with a wide range of herbivorous and carnivorous species. The aim is to contribute to the understanding of hunting behaviour, resource partitioning, diet and habitat use of animals and Neanderthals through a palaeoecological reconstruction. 相似文献
70.
Multi-environment analysis and improved mapping of a yield-related QTL on chromosome 3B of wheat 总被引:1,自引:0,他引:1
Julien Bonneau Julian Taylor Boris Parent Dion Bennett Matthew Reynolds Catherine Feuillet Peter Langridge Diane Mather 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2013,126(3):747-761
Improved mapping, multi-environment quantitative trait loci (QTL) analysis and dissection of allelic effects were used to define a QTL associated with grain yield, thousand grain weight and early vigour on chromosome 3BL of bread wheat (Triticum aestivum L.) under abiotic stresses. The QTL had pleiotropic effects and showed QTL x environment interactions across 21 diverse environments in Australia and Mexico. The occurrence and the severity of water deficit combined with high temperatures during the growing season affected the responsiveness of this QTL, resulting in a reversal in the direction of allelic effects. The influence of this QTL can be substantial, with the allele from one parent (RAC875) increasing grain yield by up to 12.5 % (particularly in environments where both heat and drought stress occurred) and the allele from the other parent (Kukri) increasing grain yield by up to 9 % in favourable environments. With the application of additional markers and the genotyping of additional recombinant inbred lines, the genetic map in the QTL region was refined to provide a basis for future positional cloning. 相似文献