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901.
Ana Pinheiro Isabel G. Fernández de Mera Paulo C. Alves Christian Gortázar José de la Fuente Pedro J. Esteves 《Immunogenetics》2013,65(11):777-784
Among mammals, the European rabbit (Oryctolagus cuniculus) has a unique mechanism of generating the primary antibody repertoire. Despite having over 200 VH genes, the VH1 gene, the most d-proximal VH gene, is used in 80–90 % of VDJ rearrangements, while the remaining 10–20 % is encoded by the VHn genes that map at least 100 Kb upstream of VH1. The maintenance of the VHn genes usage in low frequency in VDJ rearrangements has been suggested to represent a relic of an ancestral immunologic response to pathogens. To address this question, we sequenced VDJ genes for another leporid, genus Lepus, which separated from European rabbit 12 million years ago. Approximately 25 VDJ gene sequences were obtained for each one of three Lepus europaeus individuals. We found that Lepus also uses the VHn genes in 5–10 % of its VDJ rearrangements. Our results show that the VHn genes are a conserved ancestral polymorphism that has been maintained in the leporids genome and is being used for the generation of VDJ rearrangements by both modern Lepus and Oryctolagus. 相似文献
902.
Juliana Pereira Lyon Rafael Reis Rezende Mariana Penido Rabelo Carlos José de Lima Leonardo Marmo Moreira 《Mycopathologia》2013,175(1-2):159-164
Photodynamic therapy (PDT) has been originally developed for the treatment of cancer, but it has been successfully employed in the treatment of infectious diseases, including fungal infections. Surfactants are amphiphilic compounds that also have antifungal properties. The present work demonstrates the synergic effect of PDT with methylene blue (MB) and LED combined with four different surfactants in the killing of Candida albicans. Subinhibitory concentrations of CTAC, HPS, SDS and Triton X-100 were tested with MB PDT. The combined therapies proved to be more efficient than PDT or surfactants separately. The best results were obtained with CTAC and HPS and PDT with MB at the concentration of 32 μg/mL. In conclusion, the combination of surfactants and PDT is an alternative antifungal treatment that can achieve more effective performance with minimal discomfort to the patient. 相似文献
903.
Maria Eugenia Ortiz Juliana Bleckwedel Raúl R. Raya Fernanda Mozzi 《Applied microbiology and biotechnology》2013,97(11):4713-4726
Polyols such as mannitol, erythritol, sorbitol, and xylitol are naturally found in fruits and vegetables and are produced by certain bacteria, fungi, yeasts, and algae. These sugar alcohols are widely used in food and pharmaceutical industries and in medicine because of their interesting physicochemical properties. In the food industry, polyols are employed as natural sweeteners applicable in light and diabetic food products. In the last decade, biotechnological production of polyols by lactic acid bacteria (LAB) has been investigated as an alternative to their current industrial production. While heterofermentative LAB may naturally produce mannitol and erythritol under certain culture conditions, sorbitol and xylitol have been only synthesized through metabolic engineering processes. This review deals with the spontaneous formation of mannitol and erythritol in fermented foods and their biotechnological production by heterofermentative LAB and briefly presented the metabolic engineering processes applied for polyol formation. 相似文献
904.
Fabio Andres Castillo Martinez Eduardo Marcos Balciunas Attilio Converti Paul D. Cotter Ricardo Pinheiro de Souza Oliveira 《Biotechnology advances》2013
Bacteriocins are ribosomally-synthesized antibacterial peptides. These compounds are produced by a broad variety of different bacteria belonging mainly to the genus Bifidobacterium, to which health promoting properties have frequently been attributed. However, despite the fact that the identification of Bifidobacterium-associated bacteriocins was first reported in 1980 and that they exhibit antimicrobial activity against pathogenic microorganisms such as Listeria monocytogenes, Clostridium perfringens, and Escherichia coli, relatively little information is still available about the antimicrobial compounds produced by strains of this genus. More detailed understanding of the action mechanisms of these antimicrobials could allow us to determine the extent to which their production contributes to the probiotic properties of specific bifidobacteria strains and, potentially, be of crucial significance for ultimate preservation of functional foods or pharmaceutical applications. Here we review what is already known about their structure, classification, mode of action, functionality, immunity, production and purification. 相似文献
905.
Elton Gargioni Grisoste Barbosa Juliana Paula Leite Silvana Regina Rockenbach Marin Juliane Prela Marinho Josirley de Fátima Corrêa Carvalho Renata Fuganti-Pagliarini José Renato Bouças Farias Norman Neumaier Francismar Corrêa Marcelino-Guimarães Maria Cristina Neves de Oliveira Kazuko Yamaguchi-Shinozaki Kazuo Nakashima Kyonoshin Maruyama Norihito Kanamori Yasunari Fujita Takuya Yoshida Alexandre Lima Nepomuceno 《Plant Molecular Biology Reporter》2013,31(3):719-730
906.
Aline Silva Braga Letícia Dragonetti Girotti Leticia Lobo de Melo Simas Juliana Gonçalves Pires Vinícius Taioqui Pelá Marília Afonso Rabelo Buzalaf 《Biofouling》2013,29(7):796-804
AbstractThis work evaluated the effects of commercial toothpastes and mouth rinses containing natural/herbal agents on biofilm viability, extracellular polysaccharide (EPS) production and on enamel demineralization in vitro. Microcosm biofilm was produced on bovine enamel for 5?days and treated daily with: Orgânico natural® (toothpaste/mouth rinse), Boni Natural Menta &; Malaleuca® (toothpaste/mouth rinse), Propolis &; Myrrh® (toothpaste), Colgate Total 12 Clean Mint® (toothpaste, positive control), Malvatricin® Plus (mouth rinse), PerioGard® (mouth rinse, positive control) or PBS (negative control). Tom’s Propolis &; Myrrh® and Colgate Total 12® toothpastes and Malvatricin® Plus and PerioGard® mouth rinses significantly reduced biofilm viability (p?0.05). Only PerioGard® had significant effects on biofilm thickness and EPS. Despite the indication that Tom’s Propolis &; Myrrh® significantly reduced lesion depth, only Colgate Total 12® significantly reduced mineral loss. Malvatricin® Plus significantly reduced mineral loss and lesion depth, as did PerioGard®. Some herbal products, Malvatricin® Plus and Tom’s Propolis &; Myrrh®, showed anticaries effects. 相似文献
907.
Bajay MM Zucchi MI Kiihl TA Batista CE Monteiro M Pinheiro JB 《American journal of botany》2011,98(4):e87-e89
PREMISE OF STUDY: Microsatellite primers were developed for castor bean (Ricinus communis L.) to investigate genetic diversity and population structure, and to provide support to germplasm management. METHODS AND RESULTS: Eleven microsatellite loci were isolated using an enrichment cloning protocol and used to characterize castor bean germplasm from the collection at the Instituto Agron?mico de Campinas (IAC). In a survey of 76 castor bean accessions, the investigated loci displayed polymorphism ranging from two to five alleles. CONCLUSIONS: The information derived from microsatellite markers led to significant gains in conserved allelic richness and provides support to the implementation of several molecular breeding strategies for castor bean. 相似文献
908.
Interleukin-21 (IL-21) is a pleiotropic cytokine that regulates T-cell, B-cell, NK-cell, and myeloid-cell functions. IL-21 binds with its cognate receptor complex, which consists of the IL-21 receptor (IL-21R) and the common gamma chain. We identified a novel IL-21R-binding molecule, WSB-1, which contains WD-40 repeats and a SOCS-box domain. WSB-1 associates with the middle part of intracytoplasmic region of IL-21R and enhances the maturation of IL-21R from N-linked glycosylated form to fully glycosylated mature form. Furthermore, WSB-1 moderates IL-21R degradation. Taken together, our present study suggests that WSB-1 has a role in the tuning of the maturation and degradation of IL-21R. 相似文献
909.
de Sousa Lima Strafacci Amanda Fernandes Camargo Juliana Bertapelli Fábio Guerra Júnior Gil 《Journal of applied genetics》2020,61(2):205-212
Journal of Applied Genetics - Williams-Beuren syndrome (WBS) is a rare genetic disease caused by a sporadic heterozygous microdeletion in 7q11.23. It is characterized by distinctive facial... 相似文献
910.
Clara Degos Lisiena Hysenaj Gabriela Gonzalez‐Espinoza Vilma Arce‐Gorvel Aurlie Gagnaire Alexia Papadopoulos Karina Alejandra Pasquevich Stphane Mresse Juliana Cassataro Sylvie Mmet Jean‐Pierre Gorvel 《Cellular microbiology》2020,22(4)
The strategies by which intracellular pathogenic bacteria manipulate innate immunity to establish chronicity are poorly understood. Here, we show that Brucella abortus outer membrane protein Omp25 specifically binds the immune cell receptor SLAMF1 in vitro. The Omp25‐dependent engagement of SLAMF1 by B. abortus limits NF‐κB translocation in dendritic cells (DCs) with no impact on Brucella intracellular trafficking and replication. This in turn decreases pro‐inflammatory cytokine secretion and impairs DC activation. The Omp25‐SLAMF1 axis also dampens the immune response without affecting bacterial replication in vivo during the acute phase of Brucella infection in a mouse model. In contrast, at the chronic stage of infection, the Omp25/SLAMF1 engagement is essential for Brucella persistence. Interaction of a specific bacterial protein with an immune cell receptor expressed on the DC surface at the acute stage of infection is thus a powerful mechanism to support microbe settling in its replicative niche and progression to chronicity. 相似文献