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991.
Reinhard F. Stocker 《Cell and tissue research》1994,275(1):3-26
This review surveys the organization of the olfactory and gustatory systems in the imago and in the larva of Drosophila melanogaster, both at the sensory and the central level. Olfactory epithelia of the adult are located primarily on the third antennal segment (funiculus) and on the maxillary palps. About 200 basiconic (BS), 150 trichoid (TS) and 60 coeloconic sensilla (CS) cover the surface of the funiculus, and an additional 60 BS are located on the maxillary palps. Males possess about 30% more TS but 20% fewer BS than females. All these sensilla are multineuronal; they may be purely olfactory or multimodal with an olfactory component. Antennal and maxillary afferents converge onto approximately 35 glomeruli within the antennal lobe. These projections obey precise rules: individual fibers are glomerulus-specific, and different types of sensilla are associated with particular subsets of glomeruli. Possible functions of antennal glomeruli are discussed. In contrast to olfactory sensilla, gustatory sensilla of the imago are located at many sites, including the labellum, the pharynx, the legs, the wing margin and the female genitalia. Each of these sensory sites has its own central target. Taste sensilla are usually composed of one mechano-and three chemosensory neurons. Individual chemosensory neurons within a sensillum respond to distinct subsets of molecules and project into different central target regions. The chemosensory system of the larva is much simpler and consists essentially of three major sensillar complexes on the cephalic lobe, the dorsal, terminal and ventral organs, and a series of pharyngeal sensilla. 相似文献
992.
Food Biophysics - The impact of the initial oil droplet composition and physical state on molecular exchange processes in mixed oil-in-water nanoemulsions was investigated. Nanoemulsions consisting... 相似文献
993.
994.
Alexander Krah Denys Pogoryelov Julian D. Langer Peter J. Bond Thomas Meier José D. Faraldo-Gómez 《BBA》2010,1797(6-7):763-772
The functional mechanism of the F1Fo ATP synthase, like many membrane transporters and pumps, entails a conformational cycle that is coupled to the movement of H+ or Na+ ions across its transmembrane domain, down an electrochemical gradient. This coupling is an efficient means of energy transduction and regulation, provided that ion binding to the membrane domain, known as Fo, is appropriately selective. In this study we set out to establish the structural and energetic basis for the ion-binding selectivity of the membrane-embedded Fo rotors of two representative ATP synthases. First, we use a biochemical approach to demonstrate the inherent binding selectivity of these rotors, that is, independently from the rest of the enzyme. We then use atomically detailed computer simulations of wild-type and mutagenized rotors to calculate and rationalize their selectivity, on the basis of the structure, dynamics and coordination chemistry of the binding sites. We conclude that H+ selectivity is most likely a robust property of all Fo rotors, arising from the prominent presence of a conserved carboxylic acid and its intrinsic chemical propensity for protonation, as well as from the structural plasticity of the binding sites. In H+-coupled rotors, the incorporation of hydrophobic side chains to the binding sites enhances this inherent H+ selectivity. Size restriction may also favor H+ over Na+, but increasing size alone does not confer Na+ selectivity. Rather, the degree to which Fo rotors may exhibit Na+ coupling relies on the presence of a sufficient number of suitable coordinating side chains and/or structural water molecules. These ligands accomplish a shift in the relative binding energetics, which under some physiological conditions may be sufficient to provide Na+ dependence. 相似文献
995.
996.
Thomas Renger Mohamed El-Amine Madjet Marcel Schmidt am Busch Julian Adolphs Frank Müh 《Photosynthesis research》2013,116(2-3):367-388
We provide a minimal model for a structure-based simulation of excitation energy transfer in pigment–protein complexes (PPCs). In our treatment, the PPC is assembled from its building blocks. The latter are defined such that electron exchange occurs only within, but not between these units. The variational principle is applied to investigate how the Coulomb interaction between building blocks changes the character of the electronic states of the PPC. In this way, the standard exciton Hamiltonian is obtained from first principles and a hierarchy of calculation schemes for the parameters of this Hamiltonian arises. Possible extensions of this approach are discussed concerning (i) the inclusion of dispersive site energy shifts and (ii) the inclusion of electron exchange between pigments. First results on electron exchange within the special pair of photosystem II of cyanobacteria and higher plants are presented and compared with earlier results on purple bacteria. In the last part of this mini-review, the coupling of electronic and nuclear degrees of freedom is considered. First, the standard exciton–vibrational Hamiltonian is parameterized with the help of a normal mode analysis of the PPC. Second, dynamical theories are discussed that exploit this Hamiltonian in the study of dissipative exciton motion. 相似文献
997.
Heparan sulfate proteoglycan synthesis and metabolism by mouse uterine epithelial cells cultured in vitro 总被引:3,自引:0,他引:3
J P Tang J Julian S R Glasser D D Carson 《The Journal of biological chemistry》1987,262(26):12832-12842
Characterization and metabolism of heparan sulfate glycosaminoglycans and proteoglycans (HSPGs) synthesized by primary cultures of mouse uterine epithelial cells are reported. HSPGs were detected in both the medium and in the cell-associated fraction, whereas glycosaminoglycans containing little or no protein (free glycosaminoglycans) were found primarily in the cell-associated fraction. The cell-associated HSPGs were relatively large (Kav = 0.1 on Superose 12), had a buoyant density in cesium chloride gradients of 1.45-1.55 g/ml, and contained heparan sulfate chains that fell into two size classes, exhibiting Kav values on Superose 12 of 0.2-0.5 and 0.7-0.8, respectively. The free glycosaminoglycan chains displayed a Kav on Superose 12 of 0.6-0.7. The secreted HSPGs were smaller (median Kav on Superose 12 of 0.28) than the cell-associated HSPGs. More than 90% of the cell-associated HSPGs contained hydrophobic portions, as evidenced by their ability to bind to octyl-Sepharose. In contrast, only 10-15% of the secreted HSPGs bound to octyl-Sepharose. HSPGs were detected at both apical and basal cell surfaces/extracellular matrices by indirect immunofluorescence in vitro and in utero and by accessibility to external proteases in vitro. It was estimated that 60-70% of the total cell-associated HSPGs were exposed at the cell surface. The HSPGs released from the cell surface by proteases were slightly smaller than the intact HSPGs and lacked the hydrophobic properties of the latter. These observations suggested that the cell surface HSPGs contain a small, hydrophobic domain that functions in the attachment of HSPGs to cells. The free glycosaminoglycans appeared to be primarily intracellular and were not secreted. The cell-associated HSPGs turned over rapidly (t1/2 = 1.5 h) and appeared to be the precursors to the free glycosaminoglycans. Metabolic turnover of the free glycosaminoglycan pool was a relatively slow process (t1/2 = 10-12 h).(ABSTRACT TRUNCATED AT 400 WORDS) 相似文献
998.
Julian K. Finn Mark D. Norman 《Proceedings. Biological sciences / The Royal Society》2010,277(1696):2967-2971
Argonauts (Cephalopoda: Argonautidae) are a group of rarely encountered open-ocean pelagic octopuses with benthic ancestry. Female argonauts inhabit a brittle ‘paper nautilus’ shell, the role of which has puzzled naturalists for millennia. The primary role attributed to the shell has been as a receptacle for egg deposition and brooding. Our observations of wild argonauts have revealed that the thin calcareous shell also functions as a hydrostatic structure, employed by the female argonaut to precisely control buoyancy at varying depths. Female argonauts use the shell to ‘gulp’ a measured volume of air at the sea surface, seal off the captured gas using flanged arms and forcefully dive to a depth where the compressed gas buoyancy counteracts body weight. This process allows the female argonaut to attain neutral buoyancy at depth and potentially adjust buoyancy to counter the increased (and significant) weight of eggs during reproductive periods. Evolution of this air-capture strategy enables this negatively buoyant octopus to survive free of the sea floor. This major shift in life mode from benthic to pelagic shows strong evolutionary parallels with the origins of all cephalopods, which attained gas-mediated buoyancy via the closed-chambered shells of the true nautiluses and their relatives. 相似文献
999.
Julian Rzóska 《Hydrobiologia》1961,17(4):265-286
Summary African rainpools in the scrub desert around Khartoum lasted from seven days to six weeks according to variable rain; they appeared in one or few series during one rainy season, interspersed and followed by drought. A rich fauna with several Euphyllopods shows a remarkable speed of development; Triops can appear in medium size in seven days; Conchostraca are mature in five days, Moina dubia and Metacyclops minutus in the first two days of a pools existence. Quantitative records show rapid repopulation of pools on dried out sites, the numerical increases are much higher than in permanent waters. The span of life of the species involved is much shorter than in species of temperate waters. In the general section attempts at classification of temporary waters are discussed; the variety of such waters in Africa, as exemplified in the Sudan, shows inadequacy of schemes made solely on the basis of European conditions. H. Gauthier's thesis that climate, amongst ecological conditions, is mainly responsible for the appearence of fresh water faunas is exemplified by the distribution of Euphyllopods in Africa.
Résumé Les mares de pluie dans le semi-désert qui entoure Khartoum durent de sept jours à six semaines selon la variation dans la chute de pluie. Elles se forment en une ou plusieurs séries pendant une seule saison de pluie, et elles sont entrecoupées et suivies par des périodes de sécheresse. Une fauna riche avec plusieurs Euphyllopodes démontre une croissance rapide. Triops de taille moyenne peuvent se former en sept jours, Conchostracés murissent en 5 jours et Moina dubia et Metacyclops minutes en 2 jours d'éxistence de mare. Les statistiques démontrent une repopulation rapide des mares qui se forment sur des terrains déssechés; l'augmentation en nombre est considáblement plus élevée que celle des eaux permanentes. La vie de l'espèce en question est beaucoup plus courte dans ces mares que dans l'eau tempérée. Dans la section générale on traite les tentatives faites pour classifier les eaux périodiques. La grande varieté de ces eaux en Afrique, et tout particulièrement au Soudan, démontre l'insuffisance des projets basés uniquement sur les conditions européennes. La thèse de H. Gauthier, que le climat, ou les conditions ecologiques, joue le plus grand rôle dans la distribution de la faune des eaux douces, est prouvée par la distribution des Euphyllopodes en Afrique.相似文献
1000.
Julian Verbov 《BMJ (Clinical research ed.)》1971,1(5739):48-49