全文获取类型
收费全文 | 7616篇 |
免费 | 685篇 |
国内免费 | 4篇 |
专业分类
8305篇 |
出版年
2023年 | 58篇 |
2022年 | 142篇 |
2021年 | 275篇 |
2020年 | 147篇 |
2019年 | 177篇 |
2018年 | 200篇 |
2017年 | 161篇 |
2016年 | 338篇 |
2015年 | 513篇 |
2014年 | 528篇 |
2013年 | 536篇 |
2012年 | 758篇 |
2011年 | 648篇 |
2010年 | 378篇 |
2009年 | 366篇 |
2008年 | 491篇 |
2007年 | 419篇 |
2006年 | 375篇 |
2005年 | 290篇 |
2004年 | 294篇 |
2003年 | 262篇 |
2002年 | 238篇 |
2001年 | 56篇 |
2000年 | 31篇 |
1999年 | 38篇 |
1998年 | 63篇 |
1997年 | 30篇 |
1996年 | 25篇 |
1995年 | 29篇 |
1994年 | 25篇 |
1993年 | 30篇 |
1992年 | 28篇 |
1991年 | 23篇 |
1990年 | 17篇 |
1989年 | 24篇 |
1988年 | 14篇 |
1987年 | 11篇 |
1986年 | 15篇 |
1985年 | 14篇 |
1984年 | 19篇 |
1983年 | 24篇 |
1982年 | 10篇 |
1981年 | 20篇 |
1980年 | 14篇 |
1979年 | 13篇 |
1978年 | 15篇 |
1977年 | 12篇 |
1976年 | 12篇 |
1975年 | 10篇 |
1968年 | 9篇 |
排序方式: 共有8305条查询结果,搜索用时 15 毫秒
891.
Background
Fitness in birds has been shown to be negatively associated with anthropogenic noise, but the underlying mechanisms remain obscure. It is however crucial to understand the mechanisms of how urban noise impinges on fitness to obtain a better understanding of the role of chronic noise in urban ecology. Here, we examine three hypotheses on how noise might reduce reproductive output in passerine birds: (H1) by impairing mate choice, (H2) by reducing territory quality and (H3) by impeding chick development.Methodology/Principal Findings
We used long-term data from an island population of house sparrows, Passer domesticus, in which we can precisely estimate fitness. We found that nests in an area affected by the noise from large generators produced fewer young, of lower body mass, and fewer recruits, even when we corrected statistically for parental genetic quality using a cross-fostering set-up, supporting H3. Also, individual females provided their young with food less often when they bred in the noisy area compared to breeding attempts by the same females elsewhere. Furthermore, we show that females reacted flexibly to increased noise levels by adjusting their provisioning rate in the short term, which suggests that noise may be a causal factor that reduces reproductive output. We rejected H1 and H2 because nestbox occupancy, parental body mass, age and reproductive investment did not differ significantly between noisy and quiet areas.Conclusions/Significance
Our results suggest a previously undescribed mechanism to explain how environmental noise can reduce fitness in passerine birds: by acoustically masking parent–offspring communication. More importantly, using a cross-fostering set-up, our results demonstrate that birds breeding in a noisy environment experience significant fitness costs. Chronic noise is omnipresent around human habitation and may produces similar fitness consequences in a wide range of urban bird species. 相似文献892.
893.
894.
Pseudomonas aeruginosa population structure revisited under environmental focus: impact of water quality and phage pressure 总被引:1,自引:0,他引:1
Selezska K Kazmierczak M Müsken M Garbe J Schobert M Häussler S Wiehlmann L Rohde C Sikorski J 《Environmental microbiology》2012,14(8):1952-1967
Pseudomonas aeruginosa attracts research attention as a common opportunistic nosocomial pathogen causing severe health problems in humans. Nevertheless, its primary habitat is the natural environment. Here, we relate the genetic diversity of 381 environmental isolates from rivers in northern Germany to ecological factors such as river system, season of sampling and different levels of water quality. From representatives of 99 environmental clones, also in comparison with 91 clinical isolates, we determined motility phenotypes, virulence factors, biofilm formation, serotype and the resistance to seven environmental P.aeruginosa phages. The integration of genetic, ecological and phenotypic data showed (i) the presence of several extended clonal complexes (ecc) which are non-uniformly distributed across different water qualities, and (ii) a correlation of the hosts' serotype composition with susceptibility towards distinct groups of environmental phages. For at least one ecc (eccB), we assumed the ecophysiological differences on environmental water adaptation and phage resistance to be so distinct as to reinforce an environmentally driven cladogenic split from the remainder of P.aeruginosa. In summary, we conclude that the majority of the microevolutionary population dynamics of P.aeruginosa were shaped by the natural environment and not by the clinical habitat. 相似文献
895.
Fekete N Gadelorge M Fürst D Maurer C Dausend J Fleury-Cappellesso S Mailänder V Lotfi R Ignatius A Sensebé L Bourin P Schrezenmeier H Rojewski MT 《Cytotherapy》2012,14(5):540-554
Background aimsThe clinical use of human mesenchymal stromal cells (MSC) requires ex vivo expansion in media containing supplements such as fetal bovine serum or, alternatively, human platelet lysate (PL).MethodsPlatelet concentrates were frozen, quarantine stored, thawed and sterile filtered to obtain PL. PL content and its effect on fibroblast–colony-forming unit (CFU-F) formation, MSC proliferation and large-scale expansion were studied.ResultsPL contained high levels of basic fibroblast growth factor (bFGF), soluble CD40L (sCD40L), vascular cell adhesion molecule-1 (VCAM-1), intercellular adhesion molecule-1 (ICAM-1), platelet-derived growth factor AA (PDGF-AA), platelet-derived growth factor AB/BB (PDGF-AB/BB), chemokine (C-C) ligand 5 (CCL5; RANTES) transforming growth factor-β1 (TGF-β1) and chemokine (C-X-C) ligand 1/2/3 (GRO), with low batch-to-batch variability, and most were stable for up to 14 days. Inhibition of PDGF-BB and bFGF decreased MSC proliferation by about 20% and 50%, respectively. The strongest inhibition (about 75%) was observed with a combination of anti-bFGF + anti-PDGF-BB and anti-bFGF + anti-TGF-β1 + anti-PDGF-BB. Interestingly, various combinations of recombinant PDGF-BB, bFGF and TGF-β1 were not sufficient to promote cell proliferation. PL from whole blood-derived pooled platelet concentrates and apheresis platelet concentrates did not differ significantly in their growth-promoting activity on MSC.ConclusionsPL enhances MSC proliferation and can be regarded as a safe tool for MSC expansion for clinical purposes. \in particular, PDGF-BB and bFGF are essential components for the growth-promoting effect of PL, but are not sufficient for MSC proliferation. 相似文献
896.
897.
Wnt7a signals through its receptor Fzd7 to activate the planar-cell-polarity pathway and drive the symmetric expansion of satellite stem cells resulting in enhanced repair of skeletal muscle. In differentiated myofibres, we observed that Wnt7a binding to Fzd7 directly activates the Akt/mTOR growth pathway, thereby inducing myofibre hypertrophy. Notably, the Fzd7 receptor complex was associated with Gα(s) and PI(3)K and these components were required for Wnt7a to activate the Akt/mTOR growth pathway in myotubes. Wnt7a-Fzd7 activation of this pathway was completely independent of IGF-receptor activation. Together, these experiments demonstrate that Wnt7a-Fzd7 activates distinct pathways at different developmental stages during myogenic lineage progression, and identify a non-canonical anabolic signalling pathway for Wnt7a and its receptor Fzd7 in skeletal muscle. 相似文献
898.
The conserved MRE11–RAD50–NBS1 (MRN) complex is an important sensor of DNA double-strand breaks (DSBs) and facilitates DNA repair by homologous recombination (HR) and end joining. Here, we identify NBS1 as a target of cyclin-dependent kinase (CDK) phosphorylation. We show that NBS1 serine 432 phosphorylation occurs in the S, G2 and M phases of the cell cycle and requires CDK activity. This modification stimulates MRN-dependent conversion of DSBs into structures that are substrates for repair by HR. Impairment of NBS1 phosphorylation not only negatively affects DSB repair by HR, but also prevents resumption of DNA replication after replication-fork stalling. Thus, CDK-mediated NBS1 phosphorylation defines a molecular switch that controls the choice of repair mode for DSBs. 相似文献
899.
Ganzella M Moreira JD Almeida RF Böhmer AE Saute JA Holmseth S Souza DO 《Purinergic signalling》2012,8(1):49-58
Overstimulation of the glutamatergic system (excitotoxicity) is involved in various acute and chronic brain diseases. Several
studies support the hypothesis that guanosine-5′-monophosphate (GMP) can modulate glutamatergic neurotransmission. The aim
of this study was to evaluate the effects of chronically administered GMP on brain cortical glutamatergic parameters in mice.
Additionally, we investigated the neuroprotective potential of the GMP treatment submitting cortical brain slices to oxygen
and glucose deprivation (OGD). Moreover, measurements of the cerebrospinal fluid (CSF) purine levels were performed after
the treatment. Mice received an oral administration of saline or GMP during 3 weeks. GMP significantly decreases the cortical
brain glutamate binding and uptake. Accordingly, GMP reduced the immunocontent of the glutamate receptors subunits, NR2A/B
and GluR1 (NMDA and AMPA receptors, respectively) and glutamate transporters EAAC1 and GLT1. GMP treatment significantly reduced
the immunocontent of PSD-95 while did not affect the content of Snap 25, GLAST and GFAP. Moreover, GMP treatment increased
the resistance of neocortex to OGD insult. The chronic GMP administration increased the CSF levels of GMP and its metabolites.
Altogether, these findings suggest a potential modulatory role of GMP on neocortex glutamatergic system by promoting functional
and plastic changes associated to more resistance of mice neocortex against an in vitro excitotoxicity event. 相似文献
900.