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71.
Rogier AM Quax Jan W Koper Pascal HP de Jong Ramona van Heerebeek Angelique E Weel Anne M Huisman Derkjen van Zeben Frank H de Jong Steven WJ Lamberts Johanna MW Hazes Richard A Feelders 《Arthritis research & therapy》2012,14(4):R195
Introduction
Genetic and disease-related factors give rise to a wide spectrum of glucocorticoid (GC) sensitivity in rheumatoid arthritis (RA). In clinical practice, GC treatment is not adapted to these differences in GC sensitivity. In vitro assessment of GC sensitivity before the start of therapy could allow more individualized GC therapy. The aim of the study was to investigate the association between in vitro and in vivo GC sensitivity in RA.Methods
Thirty-eight early and 37 established RA patients were prospectively studied. In vitro GC sensitivity was assessed with dexamethasone-induced effects on interleukin-2 (IL-2) and glucocorticoid-induced leucine zipper (GILZ) messenger RNA expression in peripheral blood mononuclear cells (PBMCs). A whole-cell dexamethasone-binding assay was used to measure number and affinity (1/KD) of glucocorticoid receptors (GRs).In vivo GC sensitivity was determined by measuring the disease activity score (DAS) and health assessment questionnaire disability index (HAQ-DI) score before and after 2 weeks of standardized GC treatment.Results
GR number was positively correlated with improvement in DAS. IL-2-EC50 and GILZ-EC50 values both had weak near-significant correlations with clinical improvement in DAS in intramuscularly treated patients only. HAQ responders had lower GILZ-EC50 values and higher GR number and KD.Conclusions
Baseline cellular in vitro glucocorticoid sensitivity is modestly associated with in vivo improvement in DAS and HAQ-DI score after GC bridging therapy in RA. Further studies are needed to evaluate whether in vitro GC sensitivity may support the development of tailor-made GC therapy in RA. 相似文献72.
Bhabananda Biswas Albert L. Juhasz Mohammad Mahmudur Rahman Ravi Naidu 《Microbial biotechnology》2020,13(2):522-534
Clays and surfactant-modified clays (organoclays) are becoming popular as pollutant sorbents due to their high reactivity and low-cost availability. However, the lack of field testing and data on ecotoxicity limits their application. Considering such aspects, this study assessed the impact of clay amendments to polycyclic aromatic hydrocarbons (PAHs)/cadmium (Cd)-contaminated soil on microbial respiration profiles (active vs. inactive cells) using redox staining and the relative abundance and diversity of bacteria and archaea. These clay products are bentonite, cationic surfactant-modified bentonite and palmitic acid-grafted surfactant-modified bentonite). After 70 days, the addition of bentonite and its modified forms altered microbial community structure mainly among dominant groups (Actinobacteria, Proteobacteria, Firmicutes and Chloroflexi) with effects varying depending on material loading to soil. Among amendments, fatty acid (palmitic acid) tailored cationic surfactant-modified bentonite proved to be microbial growth supportive and significantly increased the number of respiration-active microbial cells by 5% at a low dose of material (e.g. 1%). Even at high dose (5%), the similarity index using operational taxonomic units (OTUs) also indicates that this modified organoclay-mixed soil provided only slightly different environment than control soil, and therefore, it could offer more biocompatibility than its counterpart organoclay at similar dose (e.g. cationic surfactant-modified bentonite). This study promotes designing ‘eco-safe’ clay-based sorbents for environmental remediation. 相似文献
73.
Jessica G Borger Sheila L Brown Lisa M Connor Adam NR Cartwright Annette M Dougall Ruud HP Wilbers Peter C Cook Lucy H Jackson‐Jones Alexander T Phythian‐Adams Cecilia Johansson Daniel M Davis Benjamin G Dewals Franca Ronchese Andrew S MacDonald 《The EMBO journal》2017,36(16):2404-2418
Type 2 inflammation is a defining feature of infection with parasitic worms (helminths), as well as being responsible for widespread suffering in allergies. However, the precise mechanisms involved in T helper (Th) 2 polarization by dendritic cells (DCs) are currently unclear. We have identified a previously unrecognized role for type I IFN (IFN‐I) in enabling this process. An IFN‐I signature was evident in DCs responding to the helminth Schistosoma mansoni or the allergen house dust mite (HDM). Further, IFN‐I signaling was required for optimal DC phenotypic activation in response to helminth antigen (Ag), and efficient migration to, and localization with, T cells in the draining lymph node (dLN). Importantly, DCs generated from Ifnar1?/? mice were incapable of initiating Th2 responses in vivo. These data demonstrate for the first time that the influence of IFN‐I is not limited to antiviral or bacterial settings but also has a central role to play in DC initiation of Th2 responses. 相似文献
74.
Sixty of the 65 dairy farms with cubicle houses in the Norwegian county of Oppland were included in a field study of the management of calving in 1990. The farmers recorded the location of the cow when giving birth, farmer presence and whether assistance was given during calving, occurrence of suckling, and time after birth when cow and calf were separated. Such data were recorded for a total of 1125 calvings. About 10% occurred on pasture, while 78% of the remaining calvings took place in the cubicle-equipped section. Thirteen percent calved in a calving pen, the remaining cows being tethered at the time of calving. Thirty-two percent of the calvings took place in houses lacking a calving pen altogether. Farmers were present during 41% of the calvings. Suckling most frequently occurred after pasture calvings, and was least frequent after calvings within the cubicle-equipped section of the cowhouse. Injuries to the calf caused by trampling or contact with fittings etc. were rare, and no more common in association with calving in the cubicle-equipped section than with calving taking place with the cow isolated from the rest of the herd. All calves were removed from their dams within 24 h after birth. 相似文献
75.
Large inocula of Stenotrophomonas maltophilia VUN 10,003 were used to investigate bacterial degradation of benzo[a]pyrene and dibenz[a,h]anthracene. Although strain VUN 10,003 was capable of degrading 10–15 mg l−1 of the five-ring compounds in the presence of pyrene after 63 days, further addition of pyrene after degradation of the five-ring
polycyclic aromatic hydrocarbons (PAHs) ceased did not stimulate significant decreases in the concentration of benzo[a]pyrene or dibenz[a,h]anthracene. However, pyrene was degraded to undetectable levels 21 days after its addition. The amount of benzo[a]pyrene and dibenz[a,h]anthracene degraded by strain VUN 10,003 was not affected by the initial concentration of the compounds when tested at 25–100
mg l−1, by the accumulation of by-products from pyrene catabolism or a loss of ability by the cells to catabolise benzo[a]pyrene or dibenz[a,h]anthracene. Metabolite or by-product repression was suspected to be responsible for the inhibition: By-products from the
degradation of the five-ring compounds inhibited their further degradation. Journal of Industrial Microbiology & Biotechnology (2002) 28, 88–96 DOI: 10.1038/sj/jim/7000216
Received 30 January 2001/ Accepted in revised form 10 October 2001 相似文献
76.
Kelly Yoshizaki J?se Mára Brito Henrique T Moriya Alessandra C Toledo Sandra Ferzilan Ana Paula Ligeiro de Oliveira Isabel D Machado Sandra HP Farsky Luiz FF Silva Milton A Martins Paulo HN Saldiva Thais Mauad Mariangela Macchione 《Respiratory research》2015,16(1)
Background
Diesel exhaust particles (DEPs) are deposited into the respiratory tract and are thought to be a risk factor for the development of diseases of the respiratory system. In healthy individuals, the timing and mechanisms of respiratory tract injuries caused by chronic exposure to air pollution remain to be clarified.Methods
We evaluated the effects of chronic exposure to DEP at doses below those found in a typical bus corridor in Sao Paulo (150 μg/m3). Male BALB/c mice were divided into mice receiving a nasal instillation: saline (saline; n = 30) and 30 μg/10 μL of DEP (DEP; n = 30). Nasal instillations were performed five days a week, over a period of 90 days. Bronchoalveolar lavage (BAL) was performed, and the concentrations of interleukin (IL)-4, IL-10, IL-13 and interferon-gamma (INF-γ) were determined by ELISA-immunoassay. Assessment of respiratory mechanics was performed. The gene expression of Muc5ac in lung was evaluated by RT-PCR. The presence of IL-13, MAC2+ macrophages, CD3+, CD4+, CD8+ T cells and CD20+ B cells in tissues was analysed by immunohistochemistry. Bronchial thickness and the collagen/elastic fibers density were evaluated by morphometry. We measured the mean linear intercept (Lm), a measure of alveolar distension, and the mean airspace diameter (D0) and statistical distribution (D2).Results
DEP decreased IFN-γ levels in BAL (p = 0.03), but did not significantly alter IL-4, IL-10 and IL-13 levels. MAC2+ macrophage, CD4+ T cell and CD20+ B cell numbers were not altered; however, numbers of CD3+ T cells (p ≤ 0.001) and CD8+ T cells (p ≤ 0.001) increased in the parenchyma. Although IL-13 (p = 0.008) expression decreased in the bronchiolar epithelium, Muc5ac gene expression was not altered in the lung of DEP-exposed animals. Although respiratory mechanics, elastic and collagen density were not modified, the mean linear intercept (Lm) was increased in the DEP-exposed animals (p ≤ 0.001), and the index D2 was statistically different (p = 0.038) from the control animals.Conclusion
Our data suggest that nasal instillation of low doses of DEP over a period of 90 days results in alveolar enlargement in the pulmonary parenchyma of healthy mice.Electronic supplementary material
The online version of this article (doi:10.1186/s12931-015-0172-z) contains supplementary material, which is available to authorized users. 相似文献77.
Varadi J Lekli I Juhasz B Bacskay I Szabo G Gesztelyi R Szendrei L Varga E Bak I Foresti R Motterlini R Tosaki A 《Life sciences》2007,80(17):1619-1626
There is increasing evidence corroborating a protective role of carbon monoxide releasing molecules (CORMs) in injured tissues. Carbon monoxide (CO) carriers have been recently developed as a pharmacological tool to simulate the effect of heme oxygenase-1-derived CO. The effects of CORM-3, a water-soluble CO releaser, on the incidence of reperfusion-induced ventricular fibrillation (VF) and tachycardia (VT) were studied in isolated rat hearts. Hearts were treated with different doses of CORM-3 before the induction of 30 min global ischemia followed by 120 min reperfusion. We found that at concentrations of 25 μM and 50 μM of CORM-3 promoted a significant reduction in the incidence of VF and VT. Thus, the incidence of VF was reduced by 67% (p < 0.05) and 92% (p < 0.05) with 25 μM and 50 μM of CORM-3, respectively. The protective effect of CORM-3 on the incidence of VT followed the same pattern. The antiarrhythmic protection was associated with a marked attenuation in infarct size, significant decreases in cellular Na+ and Ca2+ gains and K+ loss. Consequently, the recovery of post-ischemic function was significantly improved. In conclusion, CORM-3 exerts beneficial effects against ischemia/reperfusion-induced injury through its abilities to release CO which mediates a cardioprotective action by regulating tissue Na+, K+, and Ca2+ levels. 相似文献
78.
79.
Karsai D Gesztelyi R Zsuga J Jakab A Szendrei L Juhasz B Bak I Szabo G Lekli I Vecsernyes M Varga E Szentmiklosi AJ Tosaki A 《Cell biochemistry and biophysics》2007,47(1):45-52
The aim of the present study was to investigate the effect of hyperthyroidism on the trans-sarcolemmal adenosine (Ado) flux
via equilibrative and nitrobenzylthioinosine (NBTI)-sensitive nucleoside transporters (ENT1) in guinea pig atria, by assessing
the change in the Ado concentration of the interstitial fluid ([Ado]ISF) under nucleoside transport blockade with NBTI. For the assessment, we applied our novel method, which estimates the change
in [Ado]ISF utilizing the altered inotropic response to N6-cyclopentyladenosine (CPA), a relative stable selective agonist of A1 Ado receptors, by providing a relative index, the equivalent concentration of CPA. Our results show an interstitial A do
accumulation upon ENT1 blockade, which was more extensive in the hyperthyroid samples (CPA concentrations equieffective with
the surplus [Ado]ISF were two to three times higher in hyperthyroid atria than in euthyroid ones, with regard to the negative inotropic effect
of CPA and Ado). This suggests an enhanced Ado influx via ENT1 in hyperthyroid atria. It is concluded that hyperthyroidism
does not alter the prevailing direction of the Ado transport, moreover intensifies the Ado influx in the guinea pig atrium. 相似文献
80.
Brigitte Boxma Guenola Ricard Angela HAM van Hoek Edouard Severing Seung-Yeo Moon-van der Staay Georg WM van der Staay Theo A van Alen Rob M de Graaf Geert Cremers Michiel Kwantes Neil R McEwan C Jamie Newbold Jean-Pierre Jouany Tadeusz Michalowski Peter Pristas Martijn A Huynen Johannes HP Hackstein 《BMC evolutionary biology》2007,7(1):1-12