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541.
Purification of galectin-3 from ovine placenta: developmentally regulated expression and immunological relevance 总被引:1,自引:1,他引:1
Iglesias MM; Rabinovich GA; Ambrosio AL; Castagna LF; Sotomayor CE; Wolfenstein-Todel C 《Glycobiology》1998,8(1):59-65
Galectins, beta-galactoside-binding lectins, are extensively distributed in
the animal kingdom and share some basic molecular properties. Galectin-3, a
member of this family, is generally associated with differentiation,
morphogenesis, and metastasis. In this study, galectin-3 was isolated from
ovine placental cotyledons round the middle of the gestation period by
lactose extraction followed by affinity chromatography on lactosyl-agarose,
and separated from galectin-1 by size exclusion chromatography on a
Superose 12 column. Under native conditions this lectin behaved as a
monomer with an apparent molecular weight of approximately 29,000 and an
isoelectric point of 9.0. The partial amino acid sequence of the peptides
obtained by tryptic digestion of this protein followed by HPLC separation
showed striking homology with other members of the galectin-3 subfamily.
Furthermore, ovine placental galectin-3 exhibited specific mitogenic
activity toward rat spleen mononuclear cells. Besides, this protein
strongly reacted with a rabbit antiserum raised against a chicken galectin.
Results obtained by Western blot analysis showed that its expression was
greatly decreased in term placenta with respect to the middle of the
gestation period, suggesting a regulated expression throughout development.
相似文献
542.
神经干细胞作为一种具有自我更新能力和多向分化潜能的细胞,它的增殖和分化受到多种源于自身或外在、邻近或远程细胞信号通路的调控,各种细胞因子及胞间通讯在神经干细胞的增殖和分化中发挥着重要的作用。近年来的多种研究表明,Notch信号通路正是这样一种可以通过相邻细胞的配体与受体相互作用,从而传递信号,进一步发挥其生物学功能的重要信号通路。该通路参与了神经干细胞维持自我形态及向多种具有不同功能的神经细胞分化的过程.对于研究神经干细胞的增殖和分化具有巨大的意义。该文将就当前Notch信号通路对神经干细胞增殖分化影响的相关研究进行简要综述。 相似文献
543.
正交试验法优选超声提取知母皂甙的研究 总被引:1,自引:0,他引:1
用正交实验法对知母(Anemarrhena asphodeloides Bge.)中总皂甙的超声提取工艺进行了研究,采用正交表L9(34),以提取剂的体积分数、料液比(mA∶VB)、提取时间以及提取次数为因素,以知母总皂甙元的含量为指标,用紫外分光光度计法进行检测,所得提取液经大孔树脂纯化,得到的最佳提取参数和结果为:以φ(乙醇)=75%的水溶液为提取剂、提取料液比mA∶VB=1∶8、提取次数2次、每次30min,提取率92.8%,纯度91.0%。结果证明了对知母而言,超声提取法是一种提取效率高、操作简便、省时的方法。 相似文献
544.
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546.
CRF是威胁人类健康及生命的常见病之一,近年来平均每年以约8%的速度在增长。依靠慢性肾功能衰竭肾脏母体及机体的再生潜能在脱细胞基质支架上修复重建肾脏结构与功能,这将是慢性肾功能衰竭治疗的一种全新的途径。而去细胞基质在组织工程、干细胞及再生医学的大量应用为解决组织器官的修复和重建等难题带来了希望。本文就目前CRF的治疗现状及、肾脏组织工程研究前景进行简要综述。 相似文献
547.
Junhui Wang Shenghua Zhu Hongxing Wang Jue He Yanbo Zhang Abulimiti Adilijiang Handi Zhang Kelly Hartle Huining Guo Jiming Kong Qingjun Huang Xin‐Min Li 《Journal of neurochemistry》2014,130(6):780-789
Previous studies have demonstrated that quetiapine (QTP) may have neuroprotective properties; however, the underlying mechanisms have not been fully elucidated. In this study, we identified a novel mechanism by which QTP increased the synthesis of ATP in astrocytes and protected GABAergic neurons from aging‐induced death. In 12‐month‐old mice, QTP significantly improved cell number of GABAegic neurons in the cortex and ameliorated anxiety‐like behaviors compared to control group. Complimentary in vitro studies showed that QTP had no direct effect on the survival of aging GABAergic neurons in culture. Astrocyte‐conditioned medium (ACM) pretreated with QTP (ACMQTP) for 24 h effectively protected GABAergic neurons against aging‐induced spontaneous cell death. It was also found that QTP boosted the synthesis of ATP from cultured astrocytes after 24 h of treatment, which might be responsible for the protective effects on neurons. Consistent with the above findings, a Rhodamine 123 test showed that ACMQTP, not QTP itself, was able to prevent the decrease in mitochondrial membrane potential in the aging neurons. For the first time, our study has provided evidence that astrocytes may be the conduit through which QTP is able to exert its neuroprotective effects on GABAergic neurons.
548.
549.
Journal of Plant Growth Regulation - Low sunlight intensity from mid-June to mid-July in Southern China is the primary environmental factor that affects maize grain development. A field trial was... 相似文献
550.
Chunyan Zhang Shanshan Zhu Li Wei Xu Yan Jing Wang Rong Quan Ruiping She Fengjiao Hu Jue Liu 《PloS one》2015,10(6)
The Cap protein of porcine circovirus type 2 (PCV2) that serves as a major host-protective immunogen was used to develop recombinant vaccines for control of PCV2-associated diseases. Growing research data have demonstrated the high effectiveness of flagellin as an adjuvant for humoral and cellular immune responses. Here, a recombinant protein was designed by fusing a modified version of bacterial flagellin to PCV2 Cap protein and expressed in a baculovirus system. When administered without adjuvant to BALB/c mice, the flagellin-Cap fusion protein elicited stronger PCV2-specific IgG antibody response, higher neutralizing antibody levels, milder histopathological changes and lower viremia, as well as higher secretion of cytokines such as TNF-α and IFN-γ that conferred better protection against virus challenge than those in the recombinant Cap alone-inoculated mice. These results suggest that the recombinant Cap protein when fused to flagellin could elicit better humoral and cellular immune responses against PCV2 infection in a mouse model, thereby acting as an attractive candidate vaccine for control of the PCV2-associated diseases. 相似文献