全文获取类型
收费全文 | 26115篇 |
免费 | 2274篇 |
国内免费 | 846篇 |
出版年
2024年 | 35篇 |
2023年 | 260篇 |
2022年 | 465篇 |
2021年 | 826篇 |
2020年 | 580篇 |
2019年 | 722篇 |
2018年 | 784篇 |
2017年 | 765篇 |
2016年 | 1037篇 |
2015年 | 1562篇 |
2014年 | 1668篇 |
2013年 | 2014篇 |
2012年 | 2420篇 |
2011年 | 2179篇 |
2010年 | 1414篇 |
2009年 | 1304篇 |
2008年 | 1542篇 |
2007年 | 1470篇 |
2006年 | 1377篇 |
2005年 | 1172篇 |
2004年 | 1099篇 |
2003年 | 949篇 |
2002年 | 912篇 |
2001年 | 273篇 |
2000年 | 180篇 |
1999年 | 218篇 |
1998年 | 247篇 |
1997年 | 203篇 |
1996年 | 148篇 |
1995年 | 144篇 |
1994年 | 127篇 |
1993年 | 130篇 |
1992年 | 111篇 |
1991年 | 105篇 |
1990年 | 74篇 |
1989年 | 68篇 |
1988年 | 69篇 |
1987年 | 49篇 |
1986年 | 46篇 |
1985年 | 65篇 |
1984年 | 55篇 |
1983年 | 41篇 |
1982年 | 44篇 |
1981年 | 46篇 |
1980年 | 34篇 |
1979年 | 27篇 |
1978年 | 26篇 |
1977年 | 23篇 |
1976年 | 20篇 |
1973年 | 19篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
101.
102.
The low ethylene yield in a cell-free ethylene-forming system from olive tree leaves ( Olea europaea L. cv. Picual) was investigated. During the incubation, 1-aminocyclopropane-1-carboxylic acid (ACC) was extensively transformed into 3-hydroxypropyl amide (HPA). Enzyme extract, Mn2+ and oxygen are responsible for this reaction. Horseradish peroxidase (EC 1.11.1.7) can substitute for the enzyme extract in this reaction. HPA formation could be one reason for the poor in vitro conversion efficiency of ACC to ethylene. 相似文献
103.
104.
A peptide (PCB1) corresponding to the proregion of the rat cysteine protease cathepsin B was synthesized and its ability to inhibit cathepsin B activity investigated. PCB1 was found to be a potent inhibitor of mature cathepsin B at pH 6.0, yielding a Ki = 0.4 nM. This inhibition obeyed slow-binding kinetics and occurred as a one-step process with a k1 = 5.2 x 10(5) M-1 s-1 and a k2 = 2.2 x 10(-4) s-1. On dropping from pH 6.0 to 4.7, Ki increased markedly, and whereas k1 remained essentially unchanged, k2 increased to 4.5 x 10(-3) s-1. Thus, the increase in Ki at lower pH is due primarily to an increased dissociation rate for the cathepsin B/PCB1 complex. At pH 4.0, the inhibition was 160-fold weaker (Ki = 64 nM) than at pH 6.0, and the propeptide appeared to behave as a classical competitive inhibitor rather than a slow-binding inhibitor. Incubation of cathepsin B with a 10-fold excess of PCB1 overnight at pH 4.0 resulted in extensive cleavage of the propetide whereas no cleavage occurred at pH 6.0, consistent with the formation of a tight complex between cathepsin B and PCB1 at the higher pH. The synthetic propeptide of cathepsin B was found to be a much weaker inhibitor of papain, a structurally similar cysteine protease, and no pH dependence was observed. Inhibition constants of 2.8 and 5.6 microM were obtained for papain inhibition by PCB1 at pH 4.0 and 6.0, respectively.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
105.
The specific binding of vasoactive intestinal peptide (VIP) to bovine thyroid plasma membranes is inhibited by guanine nucleotides. Guanosine 5-triphosphate (GTP) and the non-hydrolyzable GTP analogs guanosine 5-,-imidotriphosphate (Gpp(NH)p) and guanosine 5-O-(3-thiotriphosphate) (GTP--S) inhibited markedly the binding of VIP to its receptors. This inhibition was higher with GTP than with Gpp(NH)p and GTP--S and was due to an increase of the rate of dissociation of peptide bound to membranes. Other nucleotides did not show any effect. 相似文献
106.
A method has been developed for the study of somatostatin (SS) binding to dissociated cells from rat cerebral cortex. Binding of [125I][Tyr11]SS to cells obtained by mechanical dissociation of rat cerebral cortex was dependent on time and temperature, saturable, reversible and highly specific. Under conditions of equilibrium, i.e., 60 min at 25°C, native SS inhibited tracer binding in a dose-dependent manner. The Scatchard analysis of binding data was linear and yielded a dissociation constant of 0.60±0.08 nM with a maximal binding capacity of 160±16 fmol/mg protein. The binding of [125I][Tyr11]SS was specific as shown in experiments on tracer displacement by the native peptide, SS analogues, and unrelated peptides. 相似文献
107.
108.
109.
Mar Pérez José M. Valpuesta Miguel Medina Esteban Montejo de Garcini Jesús Avila 《Journal of neurochemistry》1996,67(3):1183-1190
Abstract: Paired helical filaments isolated from the brains of patients with Alzheimer's disease are composed of a major protein component, the microtubule-associated protein termed τ, together with other nonprotein components, including heparan, a glycosaminoglycan, the more extensively sulfated form of which is heparin. As some of these nonprotein components may modulate the assembly of τ into filamentous structures, we have analyzed the ability of the whole τ protein or some of its fragments to self-assemble in the presence of heparin. Different τ fragments, all of them containing some sequences of the tubulin-binding motif, can assemble in vitro into filaments. We have also found formation of polymers with the 18-residue-long peptide corresponding to the third tubulin-binding motif of τ. This suggests that the ability of τ for self-assembly could be localized in a short sequence of amino acids present in the tubulin-binding repeats of the τ molecule. 相似文献
110.