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101.
Rosalina Lara-Rico Claudia M. López-Badillo Jesús A. Claudio-Rizo Denis. A. Cabrera-Munguía Juan J. Becerra-Rodríguez Roberto Espinosa-Neira Brenda R. Cruz-Ortiz 《Biopolymers》2023,114(6):e23538
In this work, hydrogels based on semi-interpenetrating polymeric networks (semi-IPN) based on collagen-polyurethane-alginate were studied physicochemically and from different approaches for biomedical application. It was determined that the matrices in the hydrogel state are crosslinked by the formation of urea and amide bonds between the biopolymer chains and the polyurethane crosslinker. The increment in alginate content (0–40 wt%) significantly increases the swelling capacity, generating semi-crystalline granular structures with improved storage modulus and resistance to thermal, hydrolytic, and proteolytic degradation. The in vitro bioactivity results indicated that the composition of these novel hydrogels stimulates the metabolic activity of monocytes and fibroblasts, benefiting their proliferation; while in cancer cell lines, it was determined that the composition of these biomaterials decreases the metabolic activity of breast cancer cells after 48 h of stimulation, and for colon cancer cells their metabolic activity decreases after 72 h of contact for the hydrogel with 40 wt% alginate. The matrices show a behavior of multidose release of ketorolac, and a higher concentration of analgesic is released in the semi-IPN matrix. The inhibition capacity of Escherichia coli is higher if the polysaccharide concentration is low (10 wt%). The in vitro wound closure test (scratch test) results indicate that the hydrogel with 20 wt% alginate shows an improvement in wound closure at 15 days of contact. Finally, the bioactivity of mineralization was evaluated to demonstrate that these hydrogels can induce the formation of carbonated apatite on their surface. The engineered hydrogels show biomedical multifunctionality and they could be applied in soft and hard tissue healing strategies, anticancer therapies, and drug release devices. 相似文献
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本研究于2021年3~9月,采用目标观察和全事件记录法,对广西防城港市钦州湾八路水湿地黑翅长脚鹬(Himantopus himantopus)的繁殖习性进行全过程观察记录。黑翅长脚鹬的栖息生境主要在盐田、虾塘和鱼塘,而巢主要分布在盐田生境。共发现39巢,雌雄共同营巢,按照主要巢材将其巢分为干草巢、碎石巢、泥皮巢和牛毛毡草巢4种;巢材包括禾本科(Gramineae)和莎草科(Cyperaceae)植物以及碎石、贝壳等;巢外径为(23.3±10.7)cm,巢内径为(11.2±1.9)cm,巢深为(1.6±0.5)cm,巢高为(6.5±4.3)cm(n=39);筑巢需(3±2)d(n=6)。窝卵数2~4枚,1~2 d产1枚卵,7 d内产完满窝卵(n=6)。雌雄均参与孵卵,雄性孵卵时间比雌性长,但二者差异不显著(P> 0.05),雄性(8 550±245.9)min,雌性(7 530±263.3)min,孵卵期为(25±2)d(n=6)。育雏期(26±3)d(n=6),雌雄轮流育雏,育雏前、中期(雏鸟1~20d日龄),雌性育雏时间比雄性长,是雄性的2倍,育雏后期(雏鸟大于20 d日龄),... 相似文献
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107.
Diana Villegas-Coronado Jesús Adriana Soto-Guzman Juan Manuel Martínez-Soto Nayelli Guadalupe Teran-Saavedra Ana Maria Guzman-Partida Luz Vazquez-Moreno Ana Gloria Villalba-Villalba Amir Maldonado Irlanda Lagarda-Diaz 《化学与生物多样性》2023,20(7):e202300051
Acute monocytic leukemia is a type of myeloid leukemia that develops in monocytes. The current clinical therapies for leukemia are unsatisfactory due to their side effects and nonspecificity toward target cells. Some lectins display antitumor activity and may specifically recognize cancer cells by binding to carbohydrate structures on their surface. Therefore, this study evaluated the response of the human monocytic leukemia cell lines THP-1 to the Olneya tesota PF2 lectin. The induction of apoptosis and reactive oxygen species production in PF2-treated cells was evaluated by flow cytometry, and the lectin-THP-1 cell interaction and mitochondrial membrane potential were evaluated by confocal fluorescence microscopy. PF2 genotoxicity was evaluated by DNA fragmentation analysis via gel electrophoresis. The results showed that PF2 binds to THP-1 cells, triggers apoptosis and DNA degradation, changes the mitochondrial membrane potential, and increases reactive oxygen species levels in PF2-treated THP-1 cells. These results suggest the potential use of PF2 for developing alternative anticancer treatments with enhanced specificity. 相似文献
108.
Mengyi Ba Ruonan Chen Qiuchen Huang Yanli Song Wen Li Yuanyuan Zhang Haixin Liu Xiang Xu Weidong Zhang Zhiqiang Cai Tao Sun 《化学与生物多样性》2023,20(8):e202300350
This work firstly reported a new polycaprolactone based material functionalized with guanidinium ionic liquid (PCL-GIL) as the stationary phase with high resolution performance for capillary gas chromatography (GC). It is composed of polycaprolactone (PCL) and guanidinium ionic liquid (GIL) with amphiphilic conformation. The PCL-GIL capillary column coated by static method exhibited high column efficiency of 3942 plates/m and moderate polarity. As a result, the PCL-GIL column exhibited high-resolution capability. For a mixture of 27 analytes with a wide ranging polarity and outperformed the PCL-2OH and HP-35 columns, showing its advantageous separation capability for analytes of diverse types. Moreover, the PCL-GIL column showed high resolving capability for various positional isomers and cis-/trans-isomers, including alkylbenzenes, chlorobenzenes, naphthalenes, bromonitrobenzenes, chloronitrobenzenes, benzaldehydes, phenols, alcohols, respectively. In a word, PCL derivatized by GIL units as a new type of stationary phase has a promising future in GC separations. 相似文献
109.
Juan José Pierella Karlusich Eric Pelletier Lucie Zinger Fabien Lombard Adriana Zingone Sébastien Colin Josep M. Gasol Richard G. Dorrell Nicolas Henry Eleonora Scalco Silvia G. Acinas Patrick Wincker Colomban de Vargas Chris Bowler 《Molecular ecology resources》2023,23(1):16-40
Phytoplankton account for >45% of global primary production, and have an enormous impact on aquatic food webs and on the entire Earth System. Their members are found among prokaryotes (cyanobacteria) and multiple eukaryotic lineages containing chloroplasts. Genetic surveys of phytoplankton communities generally consist of PCR amplification of bacterial (16S), nuclear (18S) and/or chloroplastic (16S) rRNA marker genes from DNA extracted from environmental samples. However, our appreciation of phytoplankton abundance or biomass is limited by PCR-amplification biases, rRNA gene copy number variations across taxa, and the fact that rRNA genes do not provide insights into metabolic traits such as photosynthesis. Here, we targeted the photosynthetic gene psbO from metagenomes to circumvent these limitations: the method is PCR-free, and the gene is universally and exclusively present in photosynthetic prokaryotes and eukaryotes, mainly in one copy per genome. We applied and validated this new strategy with the size-fractionated marine samples collected by Tara Oceans, and showed improved correlations with flow cytometry and microscopy than when based on rRNA genes. Furthermore, we revealed unexpected features of the ecology of these ecosystems, such as the high abundance of picocyanobacterial aggregates and symbionts in the ocean, and the decrease in relative abundance of phototrophs towards the larger size classes of marine dinoflagellates. To facilitate the incorporation of psbO in molecular-based surveys, we compiled a curated database of >18,000 unique sequences. Overall, psbO appears to be a promising new gene marker for molecular-based evaluations of entire phytoplankton communities. 相似文献
110.
Solana Abraham Andrea Moyano Viviana Díaz M. Gabriela Murúa Juan Rull 《Insect Science》2023,30(4):1183-1190
Promiscuous mating systems provide the opportunity for females to bias fertilization toward particular males. However, distinguishing between male sperm competition and active female sperm choice is difficult for species with internal fertilization. Nevertheless, species that store and use sperm of different males in different storing structures and species where females are able to expel all or part of the ejaculates after copulation may be able to bias fertilization. We report a series of experiments aimed at providing evidence of female sperm choice in Euxesta eluta (Hendel), a species of ulidiid fly that expels and consumes ejaculates after copulation. We found no evidence of greater reproductive success for females mated singly, multiply with the same male, or mated multiply with different males. Female E. eluta possesses two spherical spermathecae and a bursa copulatrix for sperm storage, with a ventral receptacle. There was no significant difference in storing more sperm in spermathecae 24 h after copulation than immediately after copulation. Females mated with protein-fed males had greater reproductive success than similar females mated to protein-deprived males. Protein-fed females prevented to consume the ejaculate, retained more sperm when mated to protein-fed males than when mated to protein-deprived males. Our results suggest that female E. eluta can exert control of sperm retention of higher quality males through ejaculate ejection. 相似文献