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991.
II型单纯疱疹病毒 (HSV-2) 糖蛋白D (Glycoprotein D,gD) 在介导该病毒入侵到宿主细胞中起着关键作用。为了更好地研究gD在病毒侵入过程中的作用机制,利用杆状病毒表达系统表达了gD胞外部分区域 (1~285 aa),通过Ni-NTA亲和层析以及分子排阻层析纯化后,得到的带His标签的分泌型可溶蛋白,用悬滴法对该蛋白进行了晶体筛选,获得了高质量的晶体。晶体生长条件为0.1 mol/L Hepes缓冲液 (pH 7.2),5% (V/V) 2-甲基-2,4-戊二醇 (MPD),10% 相似文献
992.
Genome-wide regulation of 5hmC, 5mC, and gene expression by Tet1 hydroxylase in mouse embryonic stem cells 总被引:2,自引:0,他引:2
Xu Y Wu F Tan L Kong L Xiong L Deng J Barbera AJ Zheng L Zhang H Huang S Min J Nicholson T Chen T Xu G Shi Y Zhang K Shi YG 《Molecular cell》2011,42(4):451-464
DNA methylation at the 5 position of cytosine (5mC) in the mammalian genome is a key epigenetic event critical for various cellular processes. The ten-eleven translocation (Tet) family of 5mC-hydroxylases, which convert 5mC to 5-hydroxymethylcytosine (5hmC), offers a way for dynamic regulation of DNA methylation. Here we report that Tet1 binds to unmodified C or 5mC- or 5hmC-modified CpG-rich DNA through its CXXC domain. Genome-wide mapping of Tet1 and 5hmC reveals mechanisms by which Tet1 controls 5hmC and 5mC levels in mouse embryonic stem cells (mESCs). We also uncover a comprehensive gene network influenced by Tet1. Collectively, our data suggest that Tet1 controls DNA methylation both by binding to CpG-rich regions to prevent unwanted DNA methyltransferase activity, and by converting 5mC to 5hmC through hydroxylase activity. This Tet1-mediated antagonism of CpG methylation imparts differential maintenance of DNA methylation status at Tet1 targets, ultimately contributing to mESC differentiation and the onset of embryonic development. 相似文献
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995.
不同功能群的根部真菌可能会与植物差异性地互作, 并进一步影响地下真菌与植物群落构建。本研究采用Illumina Miseq测序方法检测了海南尖峰岭热带山地雨林中常见植物的根部真菌; 采用网络分析法比较了丛枝菌根(AM)真菌、外生菌根(ECM)真菌, 以及所有根部真菌与植物互作的二分网络(bipartite networks)结构特性。从槭树科、番荔枝科、夹竹桃科、冬青科、棕榈科、壳斗科、樟科和木犀科等8科植物的根系中, 检测到297,831条真菌ITS1序列, 这些序列被划为1,279个真菌分类单元(OTUs), 其中子囊菌门748个、担子菌门354个、球囊菌亚门80个, 以及未知真菌97个。核心根部真菌群落(420个OTUs)中, 至少有三类不同生态功能的真菌常见, 即丛枝菌根真菌(40个OTUs, 占总序列数23.4%)、外生菌根真菌(48个OTUs, 13.9%)和腐生型真菌(83个OTUs, 19.8%)。尖峰岭山地雨林根部真菌-植物互作网络结构特性的指标普遍显著高于/低于假定物种随机互作的零模型期待值。在群落水平, 不同功能型的根部真菌-植物互作网络表现出不同或相反的结构特性, 如丛枝菌根互作网络表现为比零模型预测值高的嵌套性和连接性, 以及比零模型低的专一性, 而外生菌根互作网络呈现出比零模型预测值低的嵌套性和连接性, 以及比零模型高的专一性。在功能群水平, 植物的生态位重叠度在AM互作网络高, 而ECM互作网络低; 真菌的生态位宽度在ECM互作网络窄, 而在AM互作网络较宽。共现(co-occurrence)网络分析进一步揭示, ECM群落的物种对资源的高度种间竞争(植物、真菌高C-score), 以及AM群落的物种无明显种间竞争(低C-score), 可能分别是形成反嵌套ECM互作网络及高嵌套AM互作网络结构的原因。上述结果说明, 尖峰岭山地雨林中至少有两种及以上的种间互作机制调节群落构建: 驱动AM互作网络冗余(nestedness)及ECM互作网络的高生态位分化(专一性)。本研究在同一个森林内探讨了不同功能型的真菌-植物互作特性, 对深入理解热带森林的物种共存机制和生态恢复具有重要意义。 相似文献
996.
This paper presents a general, process-based mass balance model (CoastMab) for total phosphorus (TP) in defined coastal areas
(at the ecosystem scale). The model is based on ordinary differential equations and calculates inflow, outflow and internal
fluxes on a monthly basis. It consists of four compartments: surface water, deep water, erosion/transportation areas for fine
sediments and accumulation areas for fine sediments. The separation between surface water and deep water is not done based
on water temperature, but on sedimentological criteria instead (from the theoretical wave base). There are algorithms for
all major internal TP fluxes (sedimentation, resuspension, diffusion, mixing and burial). Validations were performed using
data from 21 different Baltic coastal areas. The results show that the model predicts monthly TP in water and chlorophyll a very well (generally within the uncertainty bands of the empirical data). The model has also been put through sensitivity
tests, which show that the most important factor regulating the predictions of the model is generally the TP concentration
in the sea beyond the coast. The model is simple to apply, since all driving variables may be accessed from maps or monitoring
programs. The driving variables include coastal area, section area (between the defined coastal area and the adjacent sea),
mean and maximum depths, latitude (used to predict water temperatures, stratification and mixing), salinity and TP concentration
in the sea. Many of the model structures are general and could be used for areas other than those included in this study,
e.g., for open coasts, estuaries or tidal coasts, as well as for other substances than phosphorus. 相似文献
997.
van Leeuwen BN van der Wulp AM Duijnstee I van Maris AJ Straathof AJ 《Applied microbiology and biotechnology》2012,93(4):1377-1387
Isobutene (2-methylpropene) is one of those chemicals for which bio-based production might replace the petrochemical production
in the future. Currently, more than 10 million metric tons of isobutene are produced on a yearly basis. Even though bio-based
production might also be achieved through chemocatalytic or thermochemical methods, this review focuses on fermentative routes
from sugars. Although biological isobutene formation is known since the 1970s, extensive metabolic engineering is required
to achieve economically viable yields and productivities. Two recent metabolic engineering developments may enable anaerobic
production close to the theoretical stoichiometry of 1isobutene + 2CO2 + 2H2O per mol of glucose. One relies on the conversion of 3-hydroxyisovalerate to isobutene as a side activity of mevalonate diphosphate
decarboxylase and the other on isobutanol dehydration as a side activity of engineered oleate hydratase. The latter resembles
the fermentative production of isobutanol followed by isobutanol recovery and chemocatalytic dehydration. The advantage of
a completely biological route is that not isobutanol, but instead gaseous isobutene is recovered from the fermenter together
with CO2. The low aqueous solubility of isobutene might also minimize product toxicity to the microorganisms. Although developments
are at their infancy, the potential of a large scale fermentative isobutene production process is assessed. The production
costs estimate is 0.9 € kg−1, which is reasonably competitive. About 70% of the production costs will be due to the costs of lignocellulose hydrolysate,
which seems to be a preferred feedstock. 相似文献
998.
Mi Li Lianqing Liu Xiubin Xiao Ning Xi Yuechao Wang 《Journal of biological physics》2016,42(4):551-569
Methotrexate is a commonly used anti-cancer chemotherapy drug. Cellular mechanical properties are fundamental parameters that reflect the physiological state of a cell. However, so far the role of cellular mechanical properties in the actions of methotrexate is still unclear. In recent years, probing the behaviors of single cells with the use of atomic force microscopy (AFM) has contributed much to the field of cell biomechanics. In this work, with the use of AFM, the effects of methotrexate on the viscoelastic properties of four types of cells were quantitatively investigated. The inhibitory and cytotoxic effects of methotrexate on the proliferation of cells were observed by optical and fluorescence microscopy. AFM indenting was used to measure the changes of cellular viscoelastic properties (Young’s modulus and relaxation time) by using both conical tip and spherical tip, quantitatively showing that the stimulation of methotrexate resulted in a significant decrease of both cellular Young’s modulus and relaxation times. The morphological changes of cells induced by methotrexate were visualized by AFM imaging. The study improves our understanding of methotrexate action and offers a novel way to quantify drug actions at the single-cell level by measuring cellular viscoelastic properties, which may have potential impacts on developing label-free methods for drug evaluation. 相似文献
999.
1000.
用RDA技术寻找肝再生相关基因的初步研究 总被引:2,自引:0,他引:2
利用表达性差异显示分析 (RDA)技术 ,研究大鼠 2 /3肝部分切除后 1h肝组织中基因的选择性表达 ,建立了大鼠 2 /3肝部分切除术后 1h再生肝组织选择性表达基因EST库。该库约含 3× 10 4 个独立克隆 ,其中 95 %以上的克隆含有插入片段 ,长度约 2 0 0~ 70 0bp不等 ,对随机挑出的 5 2个克隆的序列分析表明其中大多数基因与肝再生调控相关 (38/5 2 )。 10株未报道序列经RNA杂交证实 ,其中 6株与肝再生相关。 相似文献