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61.
Olivier Maurin Artemis Anest Sidonie Bellot Edward Biffin Grace Brewer Tristan Charles-Dominique Robyn S. Cowan Steven Dodsworth Niroshini Epitawalage Berta Gallego Augusto Giaretta Renato Goldenberg Deise J.P. Gonçalves Shirley Graham Peter Hoch Fiorella Mazine Yee Wen Low Catherine McGinnie Fabián A. Michelangeli Sarah Morris Darin S. Penneys Oscar Alejandro Pérez Escobar Yohan Pillon Lisa Pokorny Gustavo Shimizu Vanessa G. Staggemeier Andrew H. Thornhill Kyle W. Tomlinson Ian M. Turner Thais Vasconcelos Peter G. Wilson Alexandre R. Zuntini William J. Baker Félix Forest Eve Lucas 《American journal of botany》2021,108(7):1087-1111
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63.
Brooke Sadler Jackson Wilborn Lilian Antunes Timothy Kuensting Andrew T. Hale Stephen R. Gannon Kevin McCall Carlos Cruchaga Matthew Harms Norine Voisin Alexandre Reymond Gerarda Cappuccio Nicola Brunetti-Pierri Marco Tartaglia Marcello Niceta Chiara Leoni Giuseppe Zampino Allison Ashley-Koch Gabe Haller 《American journal of human genetics》2021,108(1):100-114
64.
Brooke Sadler Jackson Wilborn Lilian Antunes Timothy Kuensting Andrew T. Hale Stephen R. Gannon Kevin McCall Carlos Cruchaga Matthew Harms Norine Voisin Alexandre Reymond Gerarda Cappuccio Nicola Brunetti-Pierri Marco Tartaglia Marcello Niceta Chiara Leoni Giuseppe Zampino Allison Ashley-Koch Gabe Haller 《American journal of human genetics》2021,108(2):368
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66.
Priscilla Cristine Passoni Silva Oscar Oliveira Brasil Paula Lorena Grangeira Souto Nathalia Hack Moreira Joseane Padilha da Silva Bianca Damiani Marques Silva Alexandre Floriani Ramos 《Animal Reproduction》2021,18(1)
The aim of this study was to use estrus synchronization protocols to favor fixed-time artificial insemination and consequently fixed-time embryo collection, and increase embryo production using eCG, in gits. In a cross over design, nine Piau breed gilts were subjected to 18 days of oral progesterone; P4 group did not receive any further; GnRH group received 25µg of GnRH 104 hours after the final application of P4; and eCG+GnRH group received 1000IU of eCG 24 hours after the final P4 in addition to GnRH for subsequent embryo collection, that was performed six days after first AI, by laparotomy. Artificial insemination was performed after 12 and 24 hours of estrus in P4 group, and 128 and 144 hours in GnRH and eCG+GnRH groups. The number of CL (8.6±3.9; 8.3±2.1; 26.7±15.0) and anovulatory follicles (4.3±3.7; 3.9±3.9; 17.2±9.5) was higher in the eCG+GnRH gilts (P<0.05). However, the use of 1000 IU of eCG reduced (P<0.05) the number of total structures (5.2±3.6; 5.1±3.1; 1.7±2.7), viable embryos (5.0±3.5; 4.8±3.3; 0.4±0.7), freezable embryos (3.6±3.4; 3.3±3.8; 0.1±0.3) and recovery rate (63.7±38.9; 58.6±24.7; 5.38±9.5). P4 and GnRH protocols were effective in the production and recovery of embryos. However, the use of 1000 IU of eCG, 24 hours after P4, was not effective in promoting the production of embryos, although the animals had superovulated. 相似文献
67.
Microengineered systems with iPSC-derived cardiac and hepatic cells to evaluate drug adverse effects
Hepatic and cardiac drug adverse effects are among the leading causes of attrition in drug development programs, in part due to predictive failures of current animal or in vitro models. Hepatocytes and cardiomyocytes differentiated from human induced pluripotent stem cells (iPSCs) hold promise for predicting clinical drug effects, given their human-specific properties and their ability to harbor genetically determined characteristics that underlie inter-individual variations in drug response. Currently, the fetal-like properties and heterogeneity of hepatocytes and cardiomyocytes differentiated from iPSCs make them physiologically different from their counterparts isolated from primary tissues and limit their use for predicting clinical drug effects. To address this hurdle, there have been ongoing advances in differentiation and maturation protocols to improve the quality and use of iPSC-differentiated lineages. Among these are in vitro hepatic and cardiac cellular microsystems that can further enhance the physiology of cultured cells, can be used to better predict drug adverse effects, and investigate drug metabolism, pharmacokinetics, and pharmacodynamics to facilitate successful drug development. In this article, we discuss how cellular microsystems can establish microenvironments for these applications and propose how they could be used for potentially controlling the differentiation of hepatocytes or cardiomyocytes. The physiological relevance of cells is enhanced in cellular microsystems by simulating properties of tissue microenvironments, such as structural dimensionality, media flow, microfluidic control of media composition, and co-cultures with interacting cell types. Recent studies demonstrated that these properties also affect iPSC differentiations and we further elaborate on how they could control differentiation efficiency in microengineered devices. In summary, we describe recent advances in the field of cellular microsystems that can control the differentiation and maturation of hepatocytes and cardiomyocytes for drug evaluation. We also propose how future research with iPSCs within engineered microenvironments could enable their differentiation for scalable evaluations of drug effects. 相似文献
68.
Kashif Aziz Khan Alexandre Marineau Priscilla Doyon Mariana Acevedo tienne Durette Anne-Claude Gingras Marc J. Servant 《PLoS pathogens》2021,17(1)
Antiviral innate immune response to RNA virus infection is supported by Pattern-Recognition Receptors (PRR) including RIG-I-Like Receptors (RLR), which lead to type I interferons (IFNs) and IFN-stimulated genes (ISG) production. Upon sensing of viral RNA, the E3 ubiquitin ligase TNF Receptor-Associated Factor-3 (TRAF3) is recruited along with its substrate TANK-Binding Kinase (TBK1), to MAVS-containing subcellular compartments, including mitochondria, peroxisomes, and the mitochondria-associated endoplasmic reticulum membrane (MAM). However, the regulation of such events remains largely unresolved. Here, we identify TRK-Fused Gene (TFG), a protein involved in the transport of newly synthesized proteins to the endomembrane system via the Coat Protein complex II (COPII) transport vesicles, as a new TRAF3-interacting protein allowing the efficient recruitment of TRAF3 to MAVS and TBK1 following Sendai virus (SeV) infection. Using siRNA and shRNA approaches, we show that TFG is required for virus-induced TBK1 activation resulting in C-terminal IRF3 phosphorylation and dimerization. We further show that the ability of the TRAF3-TFG complex to engage mTOR following SeV infection allows TBK1 to phosphorylate mTOR on serine 2159, a post-translational modification shown to promote mTORC1 signaling. We demonstrate that the activation of mTORC1 signaling during SeV infection plays a positive role in the expression of Viperin, IRF7 and IFN-induced proteins with tetratricopeptide repeats (IFITs) proteins, and that depleting TFG resulted in a compromised antiviral state. Our study, therefore, identifies TFG as an essential component of the RLR-dependent type I IFN antiviral response. 相似文献
69.
Amanda Santos Gusmão Lucas Silva Abreu Josean Fechine Tavares Humberto Fonseca de Freitas Samuel Silva da Rocha Pita Elda Gonçalves dos Santos Ivo Santana Caldas André Alexandre Vieira Eliane Oliveira Silva 《化学与生物多样性》2021,18(10):e2100493
Hundreds of millions of people worldwide are affected by Chagas’ disease caused by Trypanosoma cruzi. Since the current treatment lack efficacy, specificity, and suffers from several side-effects, novel therapeutics are mandatory. Natural products from endophytic fungi have been useful sources of lead compounds. In this study, three lactones isolated from an endophytic strain culture were in silico evaluated for rational guidance of their bioassay screening. All lactones displayed in vitro activity against T. cruzi epimastigote and trypomastigote forms. Notably, the IC50 values of (+)-phomolactone were lower than benznidazole (0.86 vs. 30.78 μM against epimastigotes and 0.41 vs. 4.88 μM against trypomastigotes). Target-based studies suggested that lactones displayed their trypanocidal activities due to T. cruzi glyceraldehyde-3-phosphate dehydrogenase (TcGAPDH) inhibition, and the binding free energy for all three TcGAPDH-lactone complexes suggested that (+)-phomolactone has a lower score value (−3.38), corroborating with IC50 assays. These results highlight the potential of these lactones for further anti-T. cruzi drug development. 相似文献
70.
Olwen M. Grace Oscar A. Pérez-Escobar Eve J. Lucas Maria S. Vorontsova Gwilym P. Lewis Barnaby E. Walker Lúcia G. Lohmann Sandra Knapp Peter Wilkie Tiina Sarkinen Iain Darbyshire Eimear Nic Lughadha Alexandre Monro Yannick Woudstra Sebsebe Demissew A. Muthama Muasya Sandra Díaz William J. Baker Alexandre Antonelli 《Trends in plant science》2021,26(5):433-441