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排序方式: 共有89条查询结果,搜索用时 125 毫秒
71.
Human Brain Lectin: A Soluble Lectin That Binds Actin 总被引:1,自引:0,他引:1
Raymonde Joubert Michel Caron Virginia Avellana-Adalid Dominique Mornet Dominique Bladier 《Journal of neurochemistry》1992,58(1):200-203
A biotinylated probe was used for detection of endogenous ligands of a human brain lectin on blotted human brain soluble proteins. Of the various proteins from brain extract resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, five reacted with the biotinylated probe. After elimination of saccharidic moieties by periodic treatment of the same extract, a single band with Mr approximately 43,000 was recognized by the lectin. This band was identified as actin using an anti-actin antibody. These results were confirmed by binding of biotinylated lectin to purified actin. 相似文献
72.
Caron M Choquet-Kastylevsky G Joubert-Caron R 《Molecular & cellular proteomics : MCP》2007,6(7):1115-1122
The increased incidence of autoantibodies in malignancies has been described since the 1970s. Thus the ability to determine molecular fingerprinting of autoantibodies (antibody signatures) may provide useful clinical diagnostic and prognostic information. This review describes the use of several proteomics approaches for the identification of antigens recognized by these autoantibodies. Serological proteome analysis combines separation of tumor cell proteins on two-dimensional gel electrophoresis gels, Western blotting with sera of patients and healthy subjects, and identification of the detected antigens by MS. Alternatively multiple affinity protein profiling combines isolation of the antigens recognized by patient antibodies by two-dimensional immunoaffinity chromatography and identification by MS/MS. The use and limitations of reverse phase protein microarrays for testing patient serum containing autoantibodies are also considered. Lastly the most important difficulty of any proteomically identified autoantibody signature is validation in patient cohorts or clinical samples. 相似文献
73.
EM-652 (SCH 57068), a third generation SERM acting as pure antiestrogen in the mammary gland and endometrium 总被引:5,自引:0,他引:5
Fernand Labrie Claude Labrie Alain Blanger Jacques Simard Sylvain Gauthier Van Luu-The Yves Mrand Vincent Giguere Bernard Candas Shouqi Luo Cline Martel Shankar Mohan Singh Marc Fournier Agns Coquet Virgile Richard Ronald Charbonneau Gilles Charpenet Andr Tremblay Gilles Tremblay Lionel Cusan Raymonde Veilleux 《The Journal of steroid biochemistry and molecular biology》1999,69(1-6):51-84
74.
Chaâbane C Corvazier E Bredoux R Dally S Raïes A Villemain A Dupuy E Enouf J Bobe R 《Biochemical and biophysical research communications》2006,345(4):1377-1385
Sarco/endoplasmic reticulum Ca(2+)ATPases (SERCAs) pump free Ca(2+) from the cytosol into the endoplasmic reticulum. The human SERCA3 family counts six members named SERCA3a to 3f. However, the exact role of these different isoforms in cellular physiology remains undetermined. In this study, we compared some physiological consequences of SERCA3b and SERCA3f overexpression in HEK-293 cells. We observed that overexpression of SERCA3b affected cell adhesion capacity associated with a major disorganization of F-actin and a decrease in focal adhesion. Furthermore, we found that SERCA3f overexpression resulted in an increase in endoplasmic reticulum stress markers (including processing of X-box-binding protein-1 (XBP-1) mRNA and expression of chaperone glucose-regulated protein 78 (GRP78)). This was associated with the activation of caspase cascade and a higher spontaneous cell death. In conclusion, these data point for the first time to distinct physiological roles of SERCA3 isoforms in cell functions. 相似文献
75.
Changes in secondary structures and acidic side chains of melibiose permease upon cosubstrates binding
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Infrared difference spectroscopy analysis of the purified melibiose permease of Escherichia coli reconstituted into liposomes was carried out as a function of the presence of the two symporter substrates (Na(+), melibiose) in either H(2)O or in D(2)O media. Essentially, the data first show that addition of Na(+) induces appearance of peaks assigned to changes in the environment and/or orientation of alpha-helical domains of purified melibiose permease. Likewise, melibiose addition in the presence of Na(+) produces peaks corresponding to additional changes of alpha-helix environment or tilt. In addition to these changes, a pair of peaks (1599 (+) cm(-1)/1576 (-) cm(-1)) appearing in the Na(+)-induced difference spectrum is assigned to the antisymmetric stretching of COO(-) groups, since they show practically no shift upon H/D exchange. It is proposed that these acidic groups participate in Na(+) co-ordination. A corresponding pair of peaks, again fairly insensitive to H/D substitution (1591 (-) cm(-1)/1567 (+) cm(-1)), appear in the melibiose-induced difference spectra, and may again be assigned to COO(-) groups. The latter carboxyl groups may correspond to part or all of the acidic residues interacting with Lys or Arg in the resting state that become free upon melibiose binding. 相似文献
76.
R. Joubert-Caron D. Lutomski F. Le Saux J. Quinonero M. Caron Y. Davenne D. Bladier 《Neurochemistry international》1995,26(6):607-613
Histological brain sections were probed with human oligoclonal lectin-like IgGs (L-IgG) purified from normal serum. In intact brain, antigenic determinants for these IgG were restricted to some blood vessel endothelial cells. By contrast, during the inflammatory reaction following a surgical injury, these determinants were detected at the cell surface of different cell types, within and near the lesion site. The cells reacting with L-IgG consisted of endothelial cell, mature astrocytes, activated microglial and ependymal cells. 相似文献
77.
Anne-Marie Aucour Raymonde Bonnefille Claude Hillaire-Marcel 《Palaeogeography, Palaeoclimatology, Palaeoecology》1999,150(3-4):179-189
The relationship between organic carbon accumulation rates and 13C/12C ratios of total organic carbon (TOC) was investigated in an highland peat bog core (Ru-3) from Equatorial Africa. This core yielded a sequence spanning the last 14 kyr and was analysed with a 100–300 yr resolution for TOC-δ13C values. The Holocene section shows contrasted TOC accumulation regimes and TOC δ13C varying between −28.5 and −19.5‰ with a few very short ‘isotopic excursions' (dated at ca. 9.3, 7.5, 4.2 ka B.P.). The organic carbon accumulation rates range from 2 to 20 mg C cm−2 yr−1. They increase when TOC becomes more depleted in 13C, notably between 12 and 9.8 ka B.P., 8.5 and 7.8 ka B.P. and after 1.6 ka B.P. Periods of restricted carbon storage correspond to heavier TOC accumulation at 9.3, and between 7.5 and 1.6 ka B.P. At the study site, the δ-variations can be related to variable C4-plant inputs, and possibly, to changes in the fractionation between CO2 and the organic carbon in C3 vascular plants. The Ru-3 record indicates restricted carbon storage during the periods of increased contribution from C4 plants and/or of decreased fractionation between CO2 and organic carbon in C3 plants. Changes in TOC-δ13C values in core Ru-3 seem to match fluctuations of East Equatorial African lakes. High lake stands correspond to low δ13C intervals and vice versa. This points to indirect climatic forcing of δ13C changes in intertropical peats. 相似文献
78.
Calcium efflux and EGTA-induced calcium release from an internal platelet membrane fraction have been studied after the oxalate-supported calcium uptake had reached steady state. Increasing external calcium concentrations stimulate the calcium efflux velocity, with an apparent half-maximal stimulation at about 5 μM outside calcium concentration and a maximal velocity of calcium efflux of . Moreover, the ratio of the liberated calcium on the loaded calcium seems to be independent of the increasing external calcium concentration. Increasing the calculated internal calcium concentration by varying the oxalate potassium concentration from 10 mM to 1 mM results in an increase of the liberated calcium from the membrane vesicles from 7.4% to 63%, respectively, without changing the calcium efflux velocity. Similar conclusions can be drawn from the observation of results from the calcium efflux and EGTA-induced calcium release methods. Moreover, calcium pump reversal does not seem to be responsible for the calcium efflux or calcium release. All these different points added to the previously described regulation of calcium efflux by the catalytic subunit of cAMP protein kinase suggest us that the mechanism of calcium liberation by the platelet membranes is different from the calcium uptake. 相似文献
79.
Axonal Transport Reversal of Acetylcholinesterase Molecular Forms in Transected Nerve 总被引:3,自引:3,他引:0
Raymonde Hässig Bertrand Tavitian Francesca Pappalardo Luigi Di Giamberardino 《Journal of neurochemistry》1991,57(6):1913-1920
Reversal of anterograde rapid axonal transport of four molecular forms of acetylcholinesterase (AChE) was studied in chick sciatic nerve during the 24-h period following a nerve transection. Reversal of AChE activity started ~1 h after nerve transection, and all the forms of the enzyme, except the monomeric ones, showed reversal of transport. The quantity of enzyme activity reversed 24 h after transection was twofold greater than that normally conveyed by retrograde transport. We observed no leakage of the enzyme at the site of the nerve transection and no reversal of AChE activity transport in the distal segment of the severed nerve, a result indicating that the material carried by retrograde axonal transport cannot be reversed by axotomy. Thus, a nerve transection induces both quantitative and qualitative changes in the retrograde axonal transport, which could serve as a signal of distal injury to the cell body. The velocity of reverse transport, measured within 6 h after transection, was found to be 213 mm/day, a value close to that of retrograde transport (200 mm/day). This suggests that the reversal taking place in severed sciatic nerve is similar to the anterograde-to-retrograde conversion process normally occurring at the nerve endings. 相似文献
80.
Albert Gelet Laura Poissonnier Jean -Yves Chapelon Raymonde Bouvier Olivier Rouviere Denis Lyonnet Jean Michel Dubernard 《Andrologie》2003,13(3):242-251