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51.
52.
Caroline Le Turdu Jean-Jacques Tiercelin Elisabeth Gibert Yves Travi Kiram-Eddine Lezzar Jean-Paul Richert Marc Massault Franoise Gasse Raymonde Bonnefille Michel Decobert Bernard Gensous Vincent Jeudy Endale Tamrat Mohammed Umer Mohammed Koen Martens Balemwal Atnafu Tesfaye Chernet David Williamson Maurice Taieb 《Palaeogeography, Palaeoclimatology, Palaeoecology》1999,150(3-4):135-177
In the central sector of the Main Ethiopian Rift, the Ziway–Shala lake basin system includes four present-day residual lakes, from north to south, lakes Ziway, Langano, Abijata, and Shala. This region of East Africa is under the influence of the Intertropical Convergence Zone seasonal migration. Thus it has been designated as a potential core site by the ERICA Project (‘Environmental Research for Intertropical Climate in Africa'). The four lakes have been subjected to strong changes in water level and water salinity at least during the Late Pleistocene. The purpose of this study is to produce a model of basin formation and sediment accumulation for this system of lakes, in order to separate the effects of climatic change from environmental variations induced by local or regional factors such as volcano-tectonic forcings. In addition to an exhaustive synthesis of available data, various investigations have been used to develop this model: 3D remote sensing, high-resolution seismics, coring, and structural, sedimentological, and hydrological field studies. New AMS radiocarbon dating helped to refine the pre-existing stratigraphic framework for this region, and basin age estimations were calculated using mean sediment accumulation rates. The history of the Ziway–Shala lake basin system has been reconstructed from the Late Pliocene–Early Pleistocene period (106 yr), mainly characterized by catastrophic explosive volcanic eruptions. The early-middle Pleistocene–Late Pleistocene period (104–106 yr) was marked by a regional volcano-tectonic paroxysm, resulting in major changes in the morphology of the area, with the formation of the Abijata, Ziway and Shala lake basins. From 0.20 Ma, the Ziway–Shala basin history is marked by the eastward migration of volcano-tectonic activity, resulting in the development of the youngest basin of the Ziway–Shala system, the Langano Basin. The joint history of sedimentation in the Ziway, Langano, Abijata, and Shala lake basins started during the early-Late Pleistocene period (101–104 yr) and is characterized from this period up to the present-day by a series of climatically controlled rises and falls of lake level. 相似文献
53.
Maria G?bel Lewin Eisele Michael M?llmann Andreas Hüttmann Patricia Johansson René Scholtysik Manuela Bergmann Raymonde Busch Hartmut D?hner Michael Hallek Till Seiler Stephan Stilgenbauer Ludger Klein-Hitpass Ulrich Dührsen Jan Dürig 《PloS one》2013,8(8)
Progranulin (Pgrn) is a 88 kDa secreted protein with pleiotropic functions including regulation of cell cycle progression, cell motility, wound repair and tumorigenesis. Using microarray based gene expression profiling we have recently demonstrated that the gene for Pgrn, granulin (GRN), is significantly higher expressed in aggressive CD38+ZAP-70+ as compared to indolent CD38−ZAP-70− chronic lymphocytic leukemia (CLL) cases. Here, we measured Pgrn plasma concentrations by enzyme-linked immunosorbent assay (ELISA) in the Essen CLL cohort of 131 patients and examined Pgrn for association with established prognostic markers and clinical outcome. We found that high Pgrn plasma levels were strongly associated with adverse risk factors including unmutated IGHV status, expression of CD38 and ZAP-70, poor risk cytogenetics (11q-, 17p-) as detected by flourescence in situ hybridization (FISH) and high Binet stage. Pgrn as well as the aforementioned risk factors were prognostic for time to first treatment and overall survival in this series. Importantly, these results could be confirmed in the independent multicentric CLL1 cohort of untreated Binet stage A patients (n = 163). Here, multivariate analysis of time to first treatment revealed that high risk Pgrn (HR = 2.06, 95%-CI = 1.13–3.76, p = 0.018), unmutated IGHV status (HR = 5.63, 95%-CI = 3.05–10.38, p<0.001), high risk as defined by the study protocol (HR = 2.06, 95%-CI = 1.09–3.89, p = 0.026) but not poor risk cytogenetics were independent prognostic markers. In summary our results suggest that Pgrn is a novel, robust and independent prognostic marker in CLL that can be easily measured by ELISA. 相似文献
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55.
The introduced parasite Anguillicoloides crassus is thought to play an important role in the decline of freshwater eel (Anguilla spp.) populations. These nematodes are known to negatively affect many fitness-related traits in eels. We used experimental infections to study the effect of A. crassus on the relative size or mass of organs, and the expression of functionally relevant genes (total of 12 parameters) that are involved in the silvering process of Anguilla anguilla. Our results showed that the liver mass, the hemoglobin α-chain, and androgen receptors α expression levels were significantly higher in infected eels, whereas the freshwater rod opsin expression level and the gut mass were significantly lower in infected eels. Our results suggested that infected eels were at a more advanced stage in the silvering process than uninfected counterparts of similar size. These results may be explained by 2 hypotheses. First, A. crassus could trigger physiological mechanisms involved in the silvering process as a side-effect of infection. Second, eels may adjust their life history traits in response to infection. The implications for eel migration and reproductive success may be either negative or positive, depending on whether the response to A. crassus infection results in an additional cost of the parasite or is due to the phenotypic plasticity of the host. 相似文献
56.
Elsy Nalleli Loría-Cervera Erika Ivett Sosa-Bibiano Liliana Estefanía Villanueva-Lizama Nicole Raymonde Van Wynsberghe Silvia Beatriz Canto-Lara José Luis Batún-Cutz Fernando José Andrade-Narváez 《Memórias do Instituto Oswaldo Cruz》2013,108(2):172-177
Peromyscus yucatanicus (Rodentia: Cricetidae) is a primary reservoir of Leishmania (Leishmania) mexicana (Kinetoplastida: Trypanosomatidae). Nitric oxide (NO) generally plays a crucial role in the containment and elimination of Leishmania. The aim of this study was to determine the amount of NO produced by P. yucatanicus infected with L. (L.) mexicana. Subclinical and clinical infections were established in P. yucatanicus through inoculation with 1 x 102
and 2.5 x 106
promastigotes, respectively. Peritoneal macrophages were cultured alone or co-cultured with lymphocytes with or without soluble Leishmania antigen. The level of NO production was determined using the Griess reaction. The amount of NO produced was significantly higher (p ≤ 0.0001) in co-cultured macrophages and lymphocytes than in macrophages cultured alone. No differences in NO production were found between P. yucatanicus with subclinical L. (L.) mexicana infections and animals with clinical infections. These results support the hypothesis that the immunological mechanisms of NO production in P. yucatanicus are similar to those described in mouse models of leishmaniasis and, despite NO production, P. yucatanicus is unable to clear the parasite infection. 相似文献
57.
Pulu Sun Clment Dgut Stphane Rty Jean‐Claude Caissard Laurence Hibrand‐Saint Oyant Aurlie Bony Saretta N. Paramita Corentin Conart Jean‐Louis Magnard Julien Jeauffre Ahmed M. Abd‐El‐Haliem Jordan Marie‐Magdelaine Tatiana Thouroude Raymonde Baltenweck Carine Tisn Fabrice Foucher Michel Haring Philippe Hugueney Robert C. Schuurink Sylvie Baudino 《The Plant journal : for cell and molecular biology》2020,104(1):185-199
Roses use a non‐canonical pathway involving a Nudix hydrolase, RhNUDX1, to synthesize their monoterpenes, especially geraniol. Here we report the characterization of another expressed NUDX1 gene from the rose cultivar Rosa x wichurana, RwNUDX1‐2. In order to study the function of the RwNUDX1‐2 protein, we analyzed the volatile profiles of an F1 progeny generated by crossing R. chinensis cv. ‘Old Blush’ with R. x wichurana. A correlation test of the volatilomes with gene expression data revealed that RwNUDX1‐2 is involved in the biosynthesis of a group of sesquiterpenoids, especially E,E‐farnesol, in addition to other sesquiterpenes. In vitro enzyme assays and heterologous in planta functional characterization of the RwNUDX1‐2 gene corroborated this result. A quantitative trait locus (QTL) analysis was performed using the data of E,E‐farnesol contents in the progeny and a genetic map was constructed based on gene markers. The RwNUDX1‐2 gene co‐localized with the QTL for E,E‐farnesol content, thereby confirming its function in sesquiterpenoid biosynthesis in R. x wichurana. Finally, in order to understand the structural bases for the substrate specificity of rose NUDX proteins, the RhNUDX1 protein was crystallized, and its structure was refined to 1.7 Å. By molecular modeling of different rose NUDX1 protein complexes with their respective substrates, a structural basis for substrate discrimination by rose NUDX1 proteins is proposed. 相似文献
58.
Raymonde Rosa Marie-Odette Prehu Marie-Claude Calvin J. Badoual D. Alix R. Girod 《Human genetics》1985,71(3):235-240
Summary Seven new homozygous cases of hereditary triosephosphate isomerase (TPI) deficiency have been detected in five unrelated families.
Two of the families originate in France, the others from Algeria, Yugoslavia, and Morocco. Only the parents coming from Algeria
and Morocco were first cousins. In the other parents no evidence of consanguinity was found. All seven patients exhibited
the same symptoms, i.e. hemolytic anemia appearing very early after birth associated with pregressive neuromuscular symptoms.
Expression of the deficiency is heterogeneous; this had previously been pointed out in the previously reported cases of TPI
deficiency. Red cell TPI activity was 3 to 4% of the normal mean in the patients and 50 to 60% in the parents. The latter
did not exhibit any clinical symptoms. The levels of red cell glycolytic intermediates and the characteristics of the mutated
TPI could be studied in four of the patients only. Substantial increases of red cell dihydroxyacetone phosphate and of fructose
1,6-diphosphate, normal Km of TPI for glyceraldehyde phosphate, and thermoinstability of the enzyme were found. In addition
the electrophoretic pattern showed no significant modification of the mobility of the TPI bands, but abnormal decreased staining
of the two more anodal bands. 相似文献
59.
60.
Lutomski D Imam-Sghiouar N Blondeau K Caron M Joubert-Caron R 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》2004,808(1):105-109
The aim of the present work was to develop a highly productive and simplified process for active human galectin-1 (Gal1) production. Gal1 is a beta-galactoside binding lectin that differentially affects biological and cellular functions such as immune surveillance and apoptosis. These effects have attracted the attention of researchers in cell biology, biochemistry and immunology. However, the production of sufficient amounts of recombinant human Gal1 (rhGal1) is needed to study of the effects of Gal1 during cell treatments. To this end, an high-yield expression of rhGal1 was achieved by high-cell density fed-batch cultivation using an exponential glycerol feeding strategy and rhGal1 was purified by a one-step purification scheme using affinity chromatography. 相似文献