全文获取类型
收费全文 | 399篇 |
免费 | 20篇 |
出版年
2023年 | 2篇 |
2022年 | 3篇 |
2021年 | 9篇 |
2020年 | 9篇 |
2019年 | 4篇 |
2018年 | 3篇 |
2017年 | 7篇 |
2016年 | 15篇 |
2015年 | 13篇 |
2014年 | 13篇 |
2013年 | 19篇 |
2012年 | 23篇 |
2011年 | 14篇 |
2010年 | 10篇 |
2009年 | 14篇 |
2008年 | 22篇 |
2007年 | 15篇 |
2006年 | 16篇 |
2005年 | 20篇 |
2004年 | 25篇 |
2003年 | 19篇 |
2002年 | 11篇 |
2001年 | 3篇 |
2000年 | 3篇 |
1999年 | 8篇 |
1998年 | 10篇 |
1997年 | 12篇 |
1996年 | 7篇 |
1995年 | 2篇 |
1994年 | 6篇 |
1993年 | 6篇 |
1992年 | 9篇 |
1991年 | 6篇 |
1990年 | 8篇 |
1989年 | 5篇 |
1988年 | 4篇 |
1987年 | 3篇 |
1984年 | 3篇 |
1983年 | 2篇 |
1982年 | 5篇 |
1980年 | 3篇 |
1977年 | 6篇 |
1975年 | 1篇 |
1974年 | 3篇 |
1973年 | 2篇 |
1972年 | 4篇 |
1971年 | 1篇 |
1970年 | 2篇 |
1968年 | 3篇 |
1967年 | 2篇 |
排序方式: 共有419条查询结果,搜索用时 31 毫秒
51.
Tavernier ML Petit E Lesur D Wadouachi A Courtois B Courtois J Michaud P 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》2008,863(2):266-272
Ion-exchange chromatography has been applied to purification of unsaturated oligoglucuronans. After an isocratic elution on a strong anion-exchange column, the collected fractions were desalted by low pressure size exclusion chromatography. However, this efficient separation was limited by the time required to desalt. So, we developed a reversed-phase chromatography method using back ionization of oligomers. Two C18 columns were tested with trifluoroacetic acid (TFA 0.7%) as eluent. Different selectivities and column stabilities were observed in this acidic condition. The scale up for semi-preparative applications enabled us to recover pure unsaturated oligoglucuronans without desalting step. 相似文献
52.
Jean-Paul Cimma Josiane Arnaud Jos Labarere Olivier Guillard Frdrique Nugues Annie Marrauld Chantal Durand Jean-Marie Farvacque Serge Pierre Bottari Shahidul Haque 《Journal of trace elements in medicine and biology》2004,17(4):249-253
The putative role of aluminium intake in young Bangladeshi children (1.5 to 4 years of age) with calcium-deficient rickets was evaluated in a non randomised controlled eight month trial. The effects of aluminium or stain-less-steel cooking pots on bone metabolism were assessed by measuring blood calcium, phosphorus, alkaline phosphatase, parathyroid hormone, 1,25 dihydroxy vitamin D, aminoterminal propeptide of type 1 collagen (PINP), cross-linked carboxyterminal telopeptide of type 1 collagen (ICTP), aluminium and albumin, and by analysis of wrist radiographs. In both groups, blood alkaline phosphatase, 1,25 dihydroxy vitamin D and aluminium decreased significantly, while serum albumin increased (p < 0.01). These results suggest that the nutrition may well be of major importance, whereas the role of aluminium appears to be insignificant. 相似文献
53.
Normal telomere length and chromosomal end capping in poly(ADP-ribose) polymerase–deficient mice and primary cells despite increased chromosomal instability
下载免费PDF全文
![点击此处可从《The Journal of cell biology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
54.
Brahim Lachili Isabelle Hininger Henri Faure Josiane Arnaud Marie-Jeanne Richard Alain Favier Anne-Marie Roussel 《Biological trace element research》2001,83(2):103-110
Iron overload could promote the generation of free radicals and result in deleterious cellular damages. A physiological increase
of oxidative stress has been observed in pregnancy. A routine iron supplement, especially a combined iron and vitamin C supplementation,
without biological justifications (low hemoglobin [Hb] and iron stores) could therefore aggravate this oxidative risk. We
investigated the effect of a daily combined iron supplementation (100 mg/d as fumarate) and vitamin C (500 mg/d as ascorbate)
for the third trimester of pregnancy on lipid peroxidation (plasma TBARS), antioxidant micronutriments (Zn, Se, retinol, vitaminE,
(β-carotene) and antioxidant metalloenzymes (RBC Cu-Zn SOD and Se-GPX). The iron-supplemented group (n=27) was compared to a control group (n=27), age and number of pregnancies matched. At delivery, all the women exhibited normal Hb and ferritin values. In the supplemented
group, plasma iron level was higher than in the control group (26.90±5.52 mmol/L) and TBARs plasma levels were significantly
enhanced (p<0.05) (3.62±0.36 vs 3.01±0.37 mmol/L). No significant changes were observed in plasma trace elements and red blood cell antioxidant
metalloenzymes. Furthermore, the α-tocopherol plasma level was lowered in the iron-supplemented groups, suggesting an increased
utilization of vitamin E.
These data show that pharmalogical doses of iron, associated with high vitamin C intakes, can result in uncontrolled lipid
peroxidation. This is predictive of adverse effects for the mother and the fetus. This study illustrates the potential harmful
effects of iron supplementation when prescribed only on the assumption of anemia and not on the bases of biological criteria. 相似文献
55.
Hafida Merzouk Sihem Madani Aziz Hichami Josiane Prost Jacques Belleville Naim A. Khan 《Obesity (Silver Spring, Md.)》2002,10(7):703-714
Objective: The long‐term effects of fetal hyperinsulinemia, time course of changes in liver and very‐low‐density lipoprotein (VLDL) lipid levels and fatty acid compositions were investigated in obese offspring of streptozotocin‐induced mildly diabetic rats. Research Methods and Procedures: Mild hyperglycemia in pregnant rats was induced by intraperitoneal injection of streptozotocin on day 5 of gestation. Control pregnant rats were injected with citrate buffer. Liver and VLDL lipids and fatty acids were analyzed in offspring at different ages. Results: At birth, obese pups had higher VLDL triglyceride levels, saturated fatty acids, and C20:4n‐6. They also had lower C18:2n‐6 proportions in VLDL triglycerides, phospholipids, and cholesteryl esters than controls pups. In 1‐month‐old male and female obese rats, VLDL and liver lipid amounts were similar to those in their respective controls; however, high levels of C18:2n‐6 and C20:4n‐6 were noted in liver and VLDL lipids. At the age of 2 months, liver and VLDL triglyceride levels were higher in obese females than in control females. Fatty acid abnormalities seen in obese rats included low C18:3n‐3 and high C22:6n‐3 proportions in liver triglycerides and phospholipids. At the age of 3 months, obese rats, both males and females, compared with control animals, had higher VLDL and hepatic lipids with reduced C20:4n‐6 levels and polyunsaturated/saturated fatty acids ratios in hepatic and VLDL triglycerides and phospholipids. Discussion: Fetal obesity, associated with alterations in VLDL lipid fatty acid composition, represents an important risk factor for adult obesity and diabetes. 相似文献
56.
Josiane Girardie 《Cell and tissue research》1967,80(3):385-412
Summary The histochemical (iron, lipopigments, acid phosphatase, leucine aminopeptidase) and cytologic (lysosomes) changes occuring during pregnancy, lactation and involution of mouse, rat, rabbit, guinea-pig mammary glands are studied by light microscopy and electron microscopy.In all the animals examined, the mammary epithelium has an intracellular digestive system which is adapted to subserve two functions. The first one is the segregation of cytoplasmic components which often precedes cellular involution. The second one is the regulation of secretory processes in the non lactating glands. This digestion of endogenous materials results in the formation of various lytic bodies: dense bodies sometimes containing ferritin, vacuolated dense bodies with membranous residues, autophagic vacuoles. The lysosomes can give large complex dense bodies like lipofuscin pigments with or without ferritin.Leucine aminopeptidase which always disappears in the mouse mammary epithelium during lactation is not present in rat, rabbit, guinea-pig mammary epithelium. In these species only the vascular tissue contains the enzyme. This observation indicates that leucine aminopeptidase does not take care of the overproduction of secretory products in the non-lactating glands.Acid phosphatase is concentrated in secretory granules and in lytic bodies: multivesicular bodies, dense bodies with ferritin, vacuolated dense bodies, lipopigments. This enzyme constitutes probably a mechanism for controlling and triggering the destruction of the secretory material with no active elimination.The iron of the mammary epithelium appears in virgin mice older than 30 weeks and in mice, rats, rabbits, guinea-pigs during glandular cells involution. This is a catabolic iron located in lysosomes. Its amount depends upon the iron content of the milk and upon the competitive secretory and catabolic activities of the glandular cells. An explanation of iron disappearance during a second pregnancy and lactation is discussed. 相似文献
57.
58.
59.
60.