全文获取类型
收费全文 | 1490篇 |
免费 | 128篇 |
国内免费 | 3篇 |
出版年
2023年 | 5篇 |
2022年 | 26篇 |
2021年 | 36篇 |
2020年 | 30篇 |
2019年 | 22篇 |
2018年 | 30篇 |
2017年 | 27篇 |
2016年 | 54篇 |
2015年 | 83篇 |
2014年 | 91篇 |
2013年 | 103篇 |
2012年 | 124篇 |
2011年 | 115篇 |
2010年 | 76篇 |
2009年 | 51篇 |
2008年 | 105篇 |
2007年 | 82篇 |
2006年 | 65篇 |
2005年 | 66篇 |
2004年 | 69篇 |
2003年 | 65篇 |
2002年 | 43篇 |
2001年 | 14篇 |
2000年 | 9篇 |
1999年 | 22篇 |
1998年 | 7篇 |
1997年 | 14篇 |
1995年 | 7篇 |
1993年 | 8篇 |
1992年 | 12篇 |
1991年 | 6篇 |
1990年 | 8篇 |
1989年 | 4篇 |
1988年 | 10篇 |
1987年 | 4篇 |
1986年 | 4篇 |
1985年 | 5篇 |
1984年 | 5篇 |
1982年 | 6篇 |
1981年 | 6篇 |
1980年 | 4篇 |
1979年 | 6篇 |
1978年 | 9篇 |
1977年 | 4篇 |
1974年 | 7篇 |
1973年 | 4篇 |
1968年 | 6篇 |
1967年 | 6篇 |
1962年 | 6篇 |
1960年 | 4篇 |
排序方式: 共有1621条查询结果,搜索用时 15 毫秒
991.
Lailvaux SP Zajitschek F Dessman J Brooks R 《Evolution; international journal of organic evolution》2011,65(11):3138-3147
Evolutionary theories of aging state that the force of natural selection declines with age, resulting in trait senescence. However, sexual selection theory predicts that costly traits that signal mate value should increase in expression as survival prospects decline. Mortality rates and fertility tend to show strong signatures of senescence, whereas sexual signaling traits increase with age, but how the expression of traits such as whole-organism performance measures that are subject to both sexual and nonsexual selection should change with age is unclear. We examined the effects of both a key life-history event (mating) and diet quality (male and female optimal diets) on aging in two whole-organism performance traits (bite force and jump take-off velocity) in male and female Teleogryllus commodus crickets. We found no evidence for diet effects on any of the measured traits. Aging effects were more evident in females than in males for both jumping and biting, and constitute a mix of senescence and terminal investment patterns depending on sex/mating class. Sex and mating therefore have important implications for resource allocation to performance traits, and hence for aging of those traits, and interactions between these two factors can result in complex changes in trait expression over individual lifetimes. 相似文献
992.
993.
Andrew N. Gray Zaoping Li Josephine Henderson-Frost Marcia B. Goldberg 《Journal of bacteriology》2014,196(3):624-632
Localization of proteins to specific sites within bacterial cells is often critical to their function. In rod-shaped bacteria, proteins involved in diverse and important cell processes localize to the cell poles. The molecular mechanisms by which these proteins are targeted to the pole, however, are poorly understood. The Shigella autotransporter protein IcsA, which is localized to the pole on the surface of the bacterium, is targeted to the pole in the cytoplasm by a mechanism that is conserved across multiple Gram-negative bacterial species and has thus served as an important and informative model for studying polar localization. We present evidence that in Escherichia coli, the establishment of polar positional information recognized by IcsA requires the activity of the cytoplasmic membrane protein insertase YidC. We show that the role of YidC in IcsA localization is independent of the cell septation and cytokinesis proteins FtsQ and FtsEX. FtsQ is required for polar localization of IcsA and, based on cross-linking studies, is inserted in the vicinity of YidC, but, we find, is not dependent on YidC for membrane insertion. FtsEX is a YidC substrate, but we find that it is not required for polar localization of IcsA. These findings indicate that polar positional information recognized by IcsA depends on one or more membrane proteins that require YidC for proper membrane insertion. 相似文献
994.
Bum Ju Ahn Hoang Le Min Wook Shin Sung-Jin Bae Eun Ji Lee Hee-Jun Wee Jong-Ho Cha Hyo-Jong Lee Hye Shin Lee Jeong Hun Kim Chang-Yeon Kim Ji Hae Seo Eng H. Lo Sejin Jeon Mi-Ni Lee Goo Taeg Oh Guo Nan Yin Ji-Kan Ryu Jun-Kyu Suh Kyu-Won Kim 《The Journal of biological chemistry》2014,289(6):3328-3338
Ninjurin1 is a homotypic adhesion molecule that contributes to leukocyte trafficking in experimental autoimmune encephalomyelitis (EAE), an animal model of multiple sclerosis. However, in vivo gene deficiency animal studies have not yet been done. Here, we constructed Ninjurin1 knock-out (KO) mice and investigated the role of Ninjurin1 on leukocyte trafficking under inflammation conditions such as EAE and endotoxin-induced uveitis. Ninjurin1 KO mice attenuated EAE susceptibility by reducing leukocyte recruitment into the injury regions of the spinal cord and showed less adhesion of leukocytes on inflamed retinal vessels in endotoxin-induced uveitis mice. Moreover, the administration of a custom-made antibody (Ab26–37) targeting the Ninjurin1 binding domain ameliorated the EAE symptoms, showing the contribution of its adhesion activity to leukocyte trafficking. In addition, we addressed the transendothelial migration (TEM) activity of bone marrow-derived macrophages and Raw264.7 cells according to the expression level of Ninjurin1. TEM activity was decreased in Ninjurin1 KO bone marrow-derived macrophages and siNinj1 Raw264.7 cells. Consistent with this, GFP-tagged mNinj1-overexpressing Raw264.7 cells increased their TEM activity. Taken together, we have clarified the contribution of Ninjurin1 to leukocyte trafficking in vivo and delineated its direct functions to TEM, emphasizing Ninjurin1 as a beneficial therapeutic target against inflammatory diseases such as multiple sclerosis. 相似文献
995.
Human adipose-derived stem cells (hASC) exhibit multilineage differentiation potential with lineage specification that is dictated by both the chemical and mechanical stimuli to which they are exposed. We have previously shown that 10% cyclic tensile strain increases hASC osteogenesis and cell-mediated calcium accretion. We have also recently shown that primary cilia are present on hASC and that chemically-induced lineage specification of hASC concurrently results in length and conformation changes of the primary cilia. Further, we have observed cilia length changes in hASC cultured within a collagen I gel in response to 10% cyclic tensile strain. We therefore hypothesize that primary cilia may play a key mechanotransduction role for hASC exposed to tensile strain. The goal of this study was to use finite element analysis (FEA) to determine strains occurring within the ciliary membrane in response to 10% tensile strain applied parallel, or perpendicular, to cilia orientation. To elucidate the mechanical environment experienced by the cilium, several lengths were modeled and evaluated based on cilia lengths measured on hASC grown under varied culture conditions. Principal tensile strains in both hASC and ciliary membranes were calculated using FEA, and the magnitude and location of maximum principal tensile strain determined. We found that maximum principal tensile strain was concentrated at the base of the cilium. In the linear elastic model, applying strain perpendicular to the cilium resulted in maximum strains within the ciliary membrane from 150% to 200%, while applying strain parallel to the cilium resulted in much higher strains, approximately 400%. In the hyperelastic model, applying strain perpendicular to the cilium resulted in maximum strains within the ciliary membrane around 30%, while applying strain parallel to the cilium resulted in much higher strains ranging from 50% to 70%. Interestingly, FEA results indicated that primary cilium length was not directly related to ciliary membrane strain. Rather, it appears that cilium orientation may be more important than cilium length in determining sensitivity of hASC to tensile strain. This is the first study to model the effects of tensile strain on the primary cilium and provides newfound insight into the potential role of the primary cilium as a mechanosensor, particularly in tensile strain and potentially a multitude of other mechanical stimuli beyond fluid shear. 相似文献
996.
Travis W. Faber Nicholas A. Pullen Josephine F.A. Fernando Elizabeth Motunrayo Kolawole Jamie J.A. McLeod Marcela Taruselli Kathryn L. Williams Kevin O. Rivera Brian O. Barnstein Daniel H. Conrad John J. Ryan 《Cellular immunology》2014
A Disintegrin and Metalloproteinase (ADAM)-10 plays critical roles in neuronal migration and distribution. Recently, ADAM10 deletion was shown to disrupt myelopoiesis. We found that inducible deletion of ADAM10 using Mx1-driven Cre recombinase for a period of three weeks resulted in mast cell hyperplasia in the skin, intestine and spleen. Mast cells express surface ADAM10 in vitro and in vivo, at high levels compared to other immune cells tested. ADAM10 is important for mast cell migration, since ADAM10-deficiency reduced c-Kit-mediated migration. As with some mast cell proteases, ADAM10 expression could be altered by the cytokine microenvironment, being inhibited by IL-10 or TGFβ1, but not by several other T cell-derived cytokines. Collectively these data show that the ADAM10 protease is an important factor in mast cell migration and tissue distribution, and can be manipulated by environmental cues. 相似文献
997.
Josephine Esaete Amy E. Eycott Jenny Reiniö Richard J. Telford Vigdis Vandvik 《Biotropica》2014,46(6):677-686
Seed banks contribute to forest regeneration after disturbance, but less is known about fern spore banks, particularly in a paleotropical context. We sampled the buried seed and fern spore bank in Mabira Forest, a 300 km2 forest in central Uganda, to explore the effect of time since disturbance. Soil cores (5 cm depth) were taken from 39 plots across three different classes of ‘recovery’: (1) not disturbed since 1950; (2) logged between 1950 and 1980; and (3) cleared for agriculture between 1970 and 1990 but reforested since. Plant emergence was monitored in a glasshouse. We predicted that the seed bank would reflect time since disturbance, with more pioneer species in recently disturbed stands, and that the fern spore bank would reflect stand age less closely due to greater dispersal capacity. We recorded a median 752 seeds per square meter, most of which were trees; the most abundant species was the invasive tree Broussonetia papyrifera. The fern spore bank was twice as dense, but 95 percent of fern spores were of one species, Christella parasitica. Tree seed density was significantly affected by time since disturbance with fewer seeds in the older stands. Herb seed density, fern spore density, and species richness for all groups were not significantly affected by time since disturbance. Neither seed bank nor fern spore bank closely resembled the aboveground vegetation. We compared our results to existing literature on seed banks in tropical forests, finding that our densities are relatively high for African forests, but low compared to the Neotropics and Australia. 相似文献
998.
999.
Craig A. Walling Michael B. Morrissey Katharina Foerster Tim H. Clutton-Brock Josephine M. Pemberton Loeske E. B. Kruuk 《Genetics》2014,198(4):1735-1749
Evolutionary theory predicts that genetic constraints should be widespread, but empirical support for their existence is surprisingly rare. Commonly applied univariate and bivariate approaches to detecting genetic constraints can underestimate their prevalence, with important aspects potentially tractable only within a multivariate framework. However, multivariate genetic analyses of data from natural populations are challenging because of modest sample sizes, incomplete pedigrees, and missing data. Here we present results from a study of a comprehensive set of life history traits (juvenile survival, age at first breeding, annual fecundity, and longevity) for both males and females in a wild, pedigreed, population of red deer (Cervus elaphus). We use factor analytic modeling of the genetic variance–covariance matrix (G) to reduce the dimensionality of the problem and take a multivariate approach to estimating genetic constraints. We consider a range of metrics designed to assess the effect of G on the deflection of a predicted response to selection away from the direction of fastest adaptation and on the evolvability of the traits. We found limited support for genetic constraint through genetic covariances between traits, both within sex and between sexes. We discuss these results with respect to other recent findings and to the problems of estimating these parameters for natural populations. 相似文献
1000.
Zachary C. Ruhe Josephine Y. Nguyen Christina M. Beck David A. Low Christopher S. Hayes 《Molecular microbiology》2014,94(2):466-481
Contact‐dependent growth inhibition (CDI) is a mode of bacterial competition orchestrated by the CdiB/CdiA family of two‐partner secretion proteins. The CdiA effector extends from the surface of CDI+ inhibitor cells, binds to receptors on neighbouring bacteria and delivers a toxin domain derived from its C‐terminal region (CdiA‐CT). Here, we show that CdiA‐CT toxin translocation requires the proton‐motive force (pmf) within target bacteria. The pmf is also critical for the translocation of colicin toxins, which exploit the energized Ton and Tol systems to cross the outer membrane. However, CdiA‐CT translocation is clearly distinct from known colicin‐import pathways because ΔtolA ΔtonB target cells are fully sensitive to CDI. Moreover, we provide evidence that CdiA‐CT toxins can be transferred into the periplasm of de‐energized target bacteria, indicating that transport across the outer membrane is independent of the pmf. Remarkably, CDI toxins transferred under de‐energized conditions remain competent to enter the target‐cell cytoplasm once the pmf is restored. Collectively, these results indicate that outer‐ and inner‐membrane translocation steps can be uncoupled, and that the pmf is required for CDI toxin transport from the periplasm to the target‐cell cytoplasm. 相似文献