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101.
Caterina R. Giner Vanessa Balagu Anders K. Krabberd Isabel Ferrera Albert Re Esther Garcs Josep M. Gasol Ramiro Logares Ramon Massana 《Molecular ecology》2019,28(5):923-935
How much temporal recurrence is present in microbial assemblages is still an unanswered ecological question. Even though marked seasonal changes have been reported for whole microbial communities, less is known on the dynamics and seasonality of individual taxa. Here, we aim at understanding microbial recurrence at three different levels: community, taxonomic group and operational taxonomic units (OTUs). For that, we focused on a model microbial eukaryotic community populating a long‐term marine microbial observatory using 18S rRNA gene data from two organismal size fractions: the picoplankton (0.2–3 µm) and the nanoplankton (3–20 µm). We have developed an index to quantify recurrence in particular taxa. We found that community structure oscillated systematically between two main configurations corresponding to winter and summer over the 10 years studied. A few taxonomic groups such as Mamiellophyceae or MALV‐III presented clear recurrence (i.e., seasonality), whereas 13%–19% of the OTUs in both size fractions, accounting for ~40% of the relative abundance, featured recurrent dynamics. Altogether, our work links long‐term whole community dynamics with that of individual OTUs and taxonomic groups, indicating that recurrent and non‐recurrent changes characterize the dynamics of microbial assemblages. 相似文献
102.
Eva Teira Ramiro Logares Alberto Gutiérrez-Barral Isabel Ferrera Marta M. Varela Xosé Anxelu G. Morán Josep M. Gasol 《Environmental microbiology》2019,21(4):1482-1496
The impact of grazing, resource competition and light on prokaryotic growth and taxonomic composition in subtropical and tropical surface waters were studied through 10 microcosm experiments conducted between 30°N and 30°S in the Atlantic, Pacific and Indian oceans. Under natural sunlight conditions, significant changes in taxonomic composition were only observed after the reduction of grazing by sample filtration in combination with a decrease in resource competition by sample dilution. Sunlight exposure significantly reduced prokaryote growth (11 ± 6%) and community richness (14 ± 4%) compared to continuous darkness but did not significantly change community composition. The largest growth inhibition after sunlight exposure occurred at locations showing deep mixed layers. The reduction of grazing had an expected and significant positive effect on growth, but caused a significant decrease in community richness (16 ± 6%), suggesting that the coexistence of many different OTUs is partly promoted by the presence of predators. Dilution of the grazer-free prokaryotic community significantly enhanced growth at the level of community, but consistently and sharply reduced the abundance of Prochlorococcus and SAR11 populations. The decline of these oligotrophic bacterial taxa following an increase in resource availability is consistent with their high specialization for exploiting the limited resources available in the oligotrophic warm ocean. 相似文献
103.
Juan M. Soto Alicia Mas Jos A. Rodrigo Tatiana Alieva Gustavo Domínguez‐Bernal 《Journal of biophotonics》2019,12(9)
In this work, we report the use of refractive index (RI) tomography for quantitative analysis of unstained DH82 cell line infected with Leishmania infantum. The cell RI is reconstructed by using a modality of optical diffraction tomography technique that employs partially coherent illumination, thus enabling inherent compatibility with conventional wide‐field microscopes. The experimental results demonstrate that the cell dry mass concentration (DMC) obtained from the RI allows for reliable detection and quantitative characterization of the infection and its temporal evolution. The RI provides important insight for studying morphological changes, particularly membrane blebbing linked to an apoptosis (cell death) process induced by the disease. Moreover, the results evidence that infected DH82 cells exhibit a higher DMC than healthy samples. These findings open up promising perspectives for clinical diagnosis of Leishmania. 相似文献
104.
105.
Fiser R Masín J Basler M Krusek J Spuláková V Konopásek I Sebo P 《The Journal of biological chemistry》2007,282(5):2808-2820
The Bordetella adenylate cyclase toxin-hemolysin (CyaA) targets phagocytes expressing the alpha(M)beta2 integrin (CD11b/CD18), permeabilizes their membranes by forming small cation-selective pores, and delivers into cells a calmodulin-activated adenylate cyclase (AC) enzyme that dissipates cytosolic ATP into cAMP. We describe here a third activity of CyaA that yields elevation of cytosolic calcium concentration ([Ca2+]i) in target cells. The CyaA-mediated [Ca2+]i increase in CD11b+ J774A.1 monocytes was inhibited by extracellular La3+ ions but not by nifedipine, SK&F 96365, flunarizine, 2-aminoethyl diphenylborinate, or thapsigargin, suggesting that influx of Ca2+ into cells was not because of receptor signaling or opening of conventional calcium channels by cAMP. Compared with intact CyaA, a CyaA-AC- toxoid unable to generate cAMP promoted a faster, albeit transient, elevation of [Ca2+]i. This was not because of cell permeabilization by the CyaA hemolysin pores, because a mutant exhibiting a strongly enhanced pore-forming activity (CyaA-E509K/E516K), but unable to deliver the AC domain into cells, was also unable to elicit a [Ca2+]i increase. Further mutations interfering with AC translocation into cells, such as proline substitutions of glutamate residues 509 or 570 or deletion of the AC domain as such, reduced or ablated the [Ca2+]i-elevating capacity of CyaA. Moreover, structural alterations within the AC domain, because of insertion of various oligopeptides, differently modulated the kinetics and extent of Ca2+ influx elicited by the respective AC- toxoids. Hence, the translocating AC polypeptide itself appears to participate in formation of a novel type of membrane path for calcium ions, contributing to action of CyaA in an unexpected manner. 相似文献
106.
Antoni Sureda Pedro Tauler Antoni Aguiló Nuria Cases Isabel Llompart Josep A. Tur Antoni Pons 《Central European Journal of Biology》2007,2(1):56-70
Intense exercise induces inflammatory-like changes and oxidative stress in immune cells. Our aim was to study the effects
of antioxidant diet supplementation on the neutrophil inflammatory response and on the tocopherol associated protein (TAP)
expression after exhaustive exercise. Fourteen male-trained amateur runners were randomly divided in two placebo and supplemented
groups. Vitamins C (152 mg/d) and E (50 mg/d) supplementation were administrated to the athletes for a month, using an almond
based isotonic and energetic beverage. Non-enriched beverage was given to the placebo group. After one month, the subjects
participated in a half-marathon race (21 km-run). Neutrophil TAP mRNA expression and markers of the inflammatory response
were determined before, immediately after, and 3 h after finishing the half-marathon race. TAP expression increased after
exercise mainly in the neutrophils of the placebo group. Exercise induced an inflammatory response in both placebo and supplemented
groups, manifested with neutrophilia, increased creatine kinase and lactate dehydrogenase serum activities, neutrophil luminol
chemiluminescence and myeloperoxidase release. Plasma malondialdehyde only increased in the placebo group after exercise.
Diet supplementation with moderate levels of antioxidant vitamins avoids plasma damage in response to exhaustive exercise
without the effects on the inflammatory process. Neutrophil degranulation and increased tocopherol associated protein could
contribute to the neutrophil protection from the oxidative stress. 相似文献
107.
108.
Josep J. Centelles Antonio Ramos-Montoya Shu Lim Sara Bassilian Laszlo G. Boros Silvia Marín Marta Cascante Wai-Nang Paul Lee 《Metabolomics : Official journal of the Metabolomic Society》2007,3(2):105-111
Traditional tracer studies of cell proliferation fail to distinguish between label enrichment due to increased DNA repair
versus DNA replication. We used the emerging stable (non-radiating) isotope-based dynamic metabolic profiling technique on
HepG2 cells to determine synthesis pathways of nucleic acids from glucose and rates of proliferation using CG-MS assay of
RNA and DNA enrichment. Comparing the isotopic enrichment curve in DNA with the theoretical curve based on cell growth, we
observed that the measured tracer enrichment was significantly higher, indicating that surplus label was acquired during DNA
repair. In particular, after the first duplication (3 days), 80.13% of the total enrichment observed corresponds to duplication
and 19.87% corresponds to DNA repair as calculated from the [1, 2-13C2]-glucose incorporation curve. Our data indicate contemporary measurements of cell proliferation rates relying on tracer incorporation
may be overestimated. 13C label was distributed between m1 (m1/Σm = 80) and m2 (m2/Σm = 14) of deoxyribose, indicating that most of the glucose carbon was acquired via direct glucose oxidation in the pentose
cycle. The stable isotope technique distinguishes rates of DNA synthesis and repair via the oxidative and non-oxidative pentose
cycle, separately, in one test, without inhibition of either process. The contribution of DNA repair in malignant cells to
isotope accumulation in deoxyribose remains to be investigated. 相似文献
109.
110.
Dalmiro Borzone Mas Patricio F. Alvarenga Pablo A. Scarabotti 《Journal of fish biology》2019,95(5):1298-1310
We analysed the influence of ecological factors, phylogenetic history and trade-offs between traits on the life-history variation among 10 loricariid species of the middle Paraná River. We measured eight life-history variables and classified the life-history strategies following the equilibrium–periodic–opportunistic (EPO) model. Principal-component analysis of life-history traits segregated species along a gradient from small opportunistic (low fecundity, low parental investment) to large equilibrium (low-medium fecundity, high parental investment) species. A clear periodic strategist was absent in the analysed assemblage. Variation partitioning by canonical phylogenetic ordination analysis showed both a component of variation uniquely explained by phylogenetic history (PH; 32.2%) and a component shared between PH and ecological factors (EF; 37%). The EPO model is a useful tool for predicting correlations among life-history traits and understanding potential demographic responses of species to environmental variation. Life-history patterns observed throughout Loricariidae suggests that this family has diversified across all three endpoint strategies of the EPO model. Our study indicates that evolutionary lineage affiliation at the level of subfamily can be a strong predictor of the life-history strategy used by each species. 相似文献