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991.
Covadonga Orejas Sergio Rossi Àurea Peralba Eva García Josep Maria Gili Heike Lippert 《Polar Biology》2013,36(1):61-72
Obelia dichotoma is a thecate hydroid with a worldwide distribution, occurring mainly on shallow water hard substrates. Since the trophic ecology of hydroids in polar waters is badly understood, the aim of the present work was to study qualitatively and quantitatively the diet of these organisms in an Arctic environment and to determine their trophic significance. For this purpose, the density of the hydroid population was documented, and simultaneously, zooplankton was sampled in two different years (1997 and 1998). Prey capture rates were estimated by analysing the gastrovascular content of the polyps in a diurnal cycle. Additionally, the digestion time of O. dichotoma was measured by laboratory feeding experiments using diatoms as food items. The analyses of the gastrovascular cavities of the polyps sampled during the diurnal cycles showed that O. dichotoma fed mainly on faecal pellets, organic matter and microalgae. Zooplankton prey was also observed, but gastrovascular contents and zooplankton abundance did not show any correlation in both years. The consumption rates of the hydroid populations differed between the 2 years. It was almost double (8.9 mg Carbon m?2) in 1998 compared to 1997 (5.5 mg Carbon m?2). The significance of the environmental variability in the feeding ecology and population dynamics of hydroids under Arctic conditions is discussed. 相似文献
992.
Manuel S. López‐Berges Concepción Hera Michael Sulyok Katja Schäfer Javier Capilla Josep Guarro Antonio Di Pietro 《Molecular microbiology》2013,87(1):49-65
Fungal pathogens provoke devastating losses in agricultural production, contaminate food with mycotoxins and give rise to life‐threatening infections in humans. The soil‐borne ascomycete Fusarium oxysporum attacks over 100 different crops and can cause systemic fusariosis in immunocompromised individuals. Here we functionally characterized VeA, VelB, VelC and LaeA, four components of the velvet protein complex which regulates fungal development and secondary metabolism. Deletion of veA, velB and to a minor extent velC caused a derepression of conidiation as well as alterations in the shape and size of microconidia. VeA and LaeA were required for full virulence of F. oxysporum on tomato plants and on immunodepressed mice. A critical contribution of velvet consists in promoting chromatin accessibility and expression of the biosynthetic gene cluster for beauvericin, a depsipeptide mycotoxin that functions as a virulence determinant. These results reveal a conserved role of the velvet complex during fungal infection on plants and mammals. 相似文献
993.
Nataša Božić Juan-Miguel Puertas Nikola Lončar Cristina Sans Duran Josep López-Santín Zoran Vujčić 《Process Biochemistry》2013,48(3):438-442
In this study, a new approach for extracellular production of recombinant α-amylase in Escherichia coli was investigated. A gene encoding a highly efficient raw-starch-digesting α-amylase from Bacillus licheniformis ATCC 9945a was cloned and expressed in E. coli. The gene encoding mature α-amylase was cloned into the pDAss expression vector, and secretion of the gene product was regulated by fusion to the signal peptide of DsbA, a well-characterized E. coli periplasmic protein. E. coli BL21 (DE3) carrying pDAss vector containing amylase gene had approximately 2.5-fold higher volumetric enzyme productivity than the natural system. The recombinant enzyme showed higher efficiency for digesting diverse raw starches when compared with the native enzyme and was similar to commercial α-amylase in its ability to hydrolyze raw starches. The properties of the recombinant enzyme demonstrate the potential of the DsbA signal peptide approach for the secretory production of the fully active, industrially important recombinant enzyme. 相似文献
994.
Trophic structure of the spider community of a Mediterranean citrus grove: A stable isotope analysis
Laia Mestre Josep Piñol José Antonio Barrientos Xavier Espadaler Katrin Brewitt Christiane Werner Christian Platner 《Basic and Applied Ecology》2013,14(5):413-422
Spiders are dominant terrestrial predators that consume a large variety of prey and engage in intraguild predation. Although the feeding habits of certain species are well known, the trophic structure of spider assemblages still needs to be investigated. Stable isotope analysis enables characterisation of trophic relationships between organisms because it tracks the energy flow in food webs and indicates the average number of trophic transfers between a given species and the base of the web, thus being a useful tool to estimate the magnitude of intraguild predation in food webs. Using this technique, we studied the trophic groups of spiders and their links within the arthropod food web of a Mediterranean organic citrus grove. We assessed the trophic positions of the 25 most common spider species relative to other arthropod predators and potential prey in the four seasons of the year, both in the canopy and on the ground. The analyses showed great seasonal variation in the isotopic signatures of some arthropod species, as well as the existence of various trophic groups and a wide range of trophic levels among spiders, even in species belonging to the same family. Differences in δ15N between spiders and the most abundant prey in the grove usually spanned two trophic levels or more. Our findings provide field evidence of widespread intraguild predation in the food web and caution against using spider families or guilds instead of individual species when studying spider trophic interactions. 相似文献
995.
María José Hernández Josep Balaguer Rocío Armero Joana Baldó Lorena Gómez Cristina Solaz 《Revista iberoamericana de micología》2013,30(3):189-192
BackgroundA peritoneal fluid with a positive culture for Candida in patients with associated clinical symptoms enables peritoneal candidiasis (PC) to be diagnosed. This etiology is related to a poor prognosis, thus, it is important to know all the risk factors and to start early an empirical treatment. The risk factors associated with this kind of peritonitis are to receive prolonged antibiotic treatment, nosocomial infection, female gender, involvement of the upper gastro-intestinal (UGI) tract, and the ocurrence of an intraoperative cardiovascular failure (CVF).AimsThe principal aim was to determine the prevalence of PC in our hospital, and the secondary aims to determine the associated risk factors.MethodsWe obtained samples from 74 patients diagnosed with peritonitis, consecutively from 2007 to 2010. Cultures were performed with the free peritoneal fluid aspirated during surgery.ResultsThe prevalence of PC obtained in our hospital was 17.6%, from which 46.15% corresponded to Candida albicans. The involvement of the UGI tract and the onset of CVF can be considered risk factors for the development of this pathology. Age, gender, nosocomial infection and previous antibiotic treatment were not related to this pathology.ConclusionsOur prevalence of PC is 17.6%. The risk factors that could predispose are the involvement of the UGI tract as the cause of peritonitis, and CVF during surgical procedure. 相似文献
996.
Karin Breugelmans Kurt Jordaens Els Adriaens Jean Paul Remon Josep?Quintana Cardona Thierry Backeljau 《ZooKeys》2013,(365):83-104
The Iberian Peninsula is a region with a high endemicity of species of the terrestrial slug subgenus Mesarion. Many of these species have been described mainly on subtle differences in their proximal genitalia. It therefore remains to be investigated 1) whether these locally diverged taxa also represent different species under a phylogenetic species concept as has been shown for other Mesarion species outside the Iberian Peninsula, and 2) how these taxa are phylogenetically related. Here, we analysed DNA sequence data of two mitochondrial (COI and 16S) genes, and of the nuclear ITS1 region, to explore the phylogenetic affinities of two of these endemic taxa, viz. Arion gilvus Torres Mínguez, 1925 and A. ponsi Quintana Cardona, 2007. We also evaluated the use of these DNA sequence data as DNA barcodes for both species. Our results showed that ITS did not allow to differentiate among most of the Mesarion molecular operational taxonomic units (MOTUs) / morphospecies in Mesarion. Yet, the overall mean p-distance among the Mesarion MOTUs / morphospecies for both mtDNA fragments (16.7% for COI, 13% for 16S) was comparable to that between A. ponsi and its closest relative A. molinae (COI: 14.2%; 16S: 16.2%) and to that between A. gilvus and its closest relative A. urbiae (COI: 14.4%; 16S: 13.4%). Hence, with respect to mtDNA divergence, both A. ponsi and A. gilvus, behave as other Mesarion species or putative species-level MOTUs and thus are confirmed as distinct ‘species’. 相似文献
997.
Pedro Tauler Antoni Aguiló Isabel Gimeno Aina Noguera Alvar Agustí Josep A. Tur 《Free radical research》2013,47(9):931-938
We have determined the effects of chronic vitamin C intake on neutrophil and lymphocyte antioxidant defences during the acute phase immune response induced by intense exercise. Blood samples were taken from 16 voluntary athletes in basal conditions, both immediately after and 1 h after a duathlon competition. Sportsmen's nutrient intakes were determined before the competition. After determining the basal plasmatic ascorbate levels, the results were analysed taking into account the vitamin C intake and their plasmatic levels. Two groups were constituted, the vitamin C supplemented group and the control group, with the dietary vitamin C intake as the only statistical difference between groups. The duathlon competition induced a significant neutrophilia, which was higher in the supplemented group. Lymphocyte antioxidant enzyme activities increased after the competition, with a higher increase in SOD activity in the control group than in the supplemented one. The competition decreased neutrophil antioxidant enzyme activities and neutrophil ascorbate concentration. The decrease in the SOD activity in the supplemented group was higher than in the control group. Finally, the duathlon competition increased the expression of MAC-1 neutrophil adhesion molecule in the supplemented group. High vitamin C intake influenced the response of neutrophils and lymphocytes to oxidative stress induced by exercise, increasing the neutrophil activation. 相似文献
998.
Núria Chapinal José Luis Ruiz-De-La-Torre Alba Cerisuelo Josep Gasa María Dolores Baucells Xavier Manteca 《Journal of applied animal welfare science : JAAWS》2013,16(2):137-153
The study housed 120 pregnant sows from Day 29 of pregnancy to 1 week before parturition either in groups of 10 with trickle feeding (TRICKLE) or in groups of 20 with an unprotected electronic sow feeding (FITMIX). The study recorded aggressive interaction on 11 nonconsecutive days. Frequency of aggressive interactions was higher in FITMIX (p < .05); yet, intense physical contact was unusual in both systems (3% of the total interactions). Conflicts in FITMIX centered largely on the feeder (83% vs. 23% for FITMIX and TRICKLE, respectively [p < .05]); in TRICKLE, they occurred mainly in the resting area (13% vs. 48% for FITMIX and TRICKLE, respectively [p < .05]). Forty-six percent of the FITMIX sows (mostly gilts and subordinates) needed assistance to adapt to the feeding system. Eventually, 8.3% of the FITMIX sows failed to adapt and had to be removed. In conclusion, although aggressive interactions were mainly of low intensity, sequential feeding appeared to make FITMIX a more competitive feeding system. 相似文献
999.
Sonia G. Rabasa Elena Granda Raquel Benavides Georges Kunstler Josep M. Espelta Romá Ogaya Josep Peñuelas Michael Scherer‐Lorenzen Wojciech Gil Wojciech Grodzki Slawomir Ambrozy Johan Bergh José A. Hódar Regino Zamora Fernando Valladares 《Global Change Biology》2013,19(8):2490-2499
Predicting climate‐driven changes in plant distribution is crucial for biodiversity conservation and management under recent climate change. Climate warming is expected to induce movement of species upslope and towards higher latitudes. However, the mechanisms and physiological processes behind the altitudinal and latitudinal distribution range of a tree species are complex and depend on each tree species features and vary over ontogenetic stages. We investigated the altitudinal distribution differences between juvenile and adult individuals of seven major European tree species along elevational transects covering a wide latitudinal range from southern Spain (37°N) to northern Sweden (67°N). By comparing juvenile and adult distributions (shifts on the optimum position and the range limits) we assessed the response of species to present climate conditions in relation to previous conditions that prevailed when adults were established. Mean temperature increased by 0.86 °C on average at our sites during the last decade compared with previous 30‐year period. Only one of the species studied, Abies alba, matched the expected predictions under the observed warming, with a maximum abundance of juveniles at higher altitudes than adults. Three species, Fagus sylvatica, Picea abies and Pinus sylvestris, showed an opposite pattern while for other three species, such as Quercus ilex, Acer pseudoplatanus and Q. petraea, we were no able to detect changes in distribution. These findings are in contrast with theoretical predictions and show that tree responses to climate change are complex and are obscured not only by other environmental factors but also by internal processes related to ontogeny and demography. 相似文献
1000.