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141.
Aging is a complex process that results in compromised biological functions of the organism and increased susceptibility to disease and death. Although the molecular basis of aging is currently being investigated in many experimental contexts, there is no consensus theory to fully explain the aging process. Epigenetic factors, including DNA methylation, histone modifications, and microRNA expression, may play central roles in controlling changes in gene expression and genomic instability during aging. In this Hot Topic review, we first examine the mechanisms by which these epigenetic factors contribute to aging in diverse eukaryotic species including experimental models of yeasts, worms, and mammals. In a second section, we will emphasize in the mammalian epigenetic alterations and how they may affect human longevity by altering stem cell function and/or somatic cell decline. The field of aging epigenetics is ripe with potential, but is still in its infancy, as new layers of complexity are emerging in the epigenetic network. As an example, we are only beginning to understand the relevance of non-coding genome to organism aging or the existence of an epigenetic memory with transgenerational inheritance. Addressing these topics will be fundamental for exploiting epigenetics phenomena as markers of aging-related diseases or as therapeutic targets. 相似文献
142.
Albright Craig D. Tsai Amy Y. Mar Mei-Heng Zeisel Steven H. 《Neurochemical research》1998,23(5):751-758
Choline availability influences long-term memory in concert with changes in the spatial organization and morphology of septal neurons, however little is known concerning the effects of choline on the hippocampus, a region of the brain also important for memory performance. Pregnant rats on gestational day 12 were fed a choline control (CT), choline supplemented (CS), or choline deficient (CD) diet for 6 days and fetal brain slices were prepared on embryonic day 18 (El8). The hippocampus in these brain slices was studied for the immunohistochemical localization of the growth-related proteins transforming growth factor beta type 1 (TGF1) and GAP43, the cytoskeletal proteins vimentin and microtubule associated protein type 1 (MAP1), and the neuronal cell marker neuron specific enolase (NSE). In control hippocampus, there was weak expression of TGF1 and vimentin proteins, but moderately intense expression of MAP1 protein. These proteins were not homogeneously distributed, but were preferentially localized to cells with large cell bodies located in the central (CA1–CA3) region of the hippocampus, and to the filamentous processes of small cells in the fimbria region. Feeding a choline-supplemented diet decreased, whereas a choline-deficient diet increased the intensity of immunohistochemical labeling for these proteins in El8 hippocampus. GAP43 and NSE were localized to peripheral nervous tissue but not hippocampus, indicating that the maturation of axons and neurite outgrowth in embryonic hippocampus were unaffected by the availability of choline in the diet. These data suggest that the availability of choline affects the differentiation of specific regions of developing hippocampus. 相似文献
143.
Grazing-induced changes in plant composition affect litter quality and nutrient cycling in flooding Pampa grasslands 总被引:5,自引:0,他引:5
Changes in plant community composition induced by vertebrate grazers have been found to either accelerate or slow C and nutrient
cycling in soil. This variation may reflect the differential effects of grazing-promoted (G+) plant species on overall litter
quality and decomposition processes. Further, site conditions associated with prior grazing history are expected to influence
litter decay and nutrient turnover. We studied how grazing-induced changes in plant life forms and species identity modified
the quality of litter inputs to soil, decomposition rate and nutrient release in a flooding Pampa grassland, Argentina. Litter
from G+ forbs and grasses (two species each) and grazing-reduced (G−) grasses (two species) was incubated in long-term grazed
and ungrazed sites. G+ species, overall, showed higher rates of decomposition and N and P release from litter. However, this
pattern was primarily driven by the low-growing, high litter-quality forbs included among G+ species. Forbs decomposed and
released nutrients faster than either G+ or G− grasses. While no consistent differences between G+ and G− grasses were observed,
patterns of grass litter decay and nutrient release corresponded with interspecific differences in phenology and photosynthetic
pathway. Litter decomposition, N release and soil N availability were higher in the grazed site, irrespective of species litter
type. Our results contradict the notion that grazing, by reducing more palatable species and promoting less palatable ones,
should decrease nutrient cycling from litter. Plant tissue quality and palatability may not unequivocally link patterns of
grazing resistance and litter decomposability within a community, especially where grazing causes major shifts in life form
composition. Thus, plant functional groups defined by species’ “responses” to grazing may only partially overlap with functional
groups based on species “effects” on C and nutrient cycling. 相似文献
144.
Corridors are often considered to promote dispersal between habitat patches. In this paper, we study whether or not corridors induce colonisation of nunataks (ice-free areas in glacier surroundings) by promoting dispersal from lowland to the nunataks. On outlet glaciers, debris originating from nunataks forms the so-called medial moraines that stretch from the nunataks down-glacier to the lowland, forming corridors of debris on the glacier. Aerial dispersal was determined with yellow sticky traps on the moraines, bare glacier and glacier foreland. Dipterans were sampled in pitfall traps on the nunataks. Flying insects that were present on the vegetated glacier foreland belonged to five orders, that is, Diptera, Hemiptera, Hymenoptera, Lepidoptera and Trichoptera. On the glacier and medial moraines, however, mainly dipterans were present, with the majority of individuals found on the moraines. Hoverflies (Syrphidae) were abundant on the moraines and on the edges of nunataks close to the moraines, but were not present on the vegetated foreland. The origin of the hoverflies is thus not the nunataks and not the lowland. Rather, they are brought in by air currents towards the glacier, where they aggregate on a land type where they have a chance of survival, although it is not habitable. Thus, we conclude that the medial moraines do not function as regular corridors but as drift fences that direct the dispersal towards the adjacent land types, that is, the nunataks and the glacier foreland. 相似文献
145.
Luis Giménez-Benavides Raúl García-Camacho José María Iriondo Adrián Escudero 《Evolutionary ecology》2011,25(4):777-794
Under climate warming, plants will undergo novel selective pressures to adjust reproductive timing. Adjustment between reproductive
phenology and environment is expected to be higher in arctic and alpine habitats because the growing season is considerably
short. As early- and late-flowering species reproduce under very different environmental conditions, selective pressures on
flowering phenology and potential effects of climate change are likely to differ between them. However, there is no agreement
on the magnitude of the benefits and costs of early- vs. late-flowering species under a global warming scenario. In spite
of its relevance, phenotypic selection on flowering phenology has rarely been explored in alpine plants and never in Mediterranean
high mountain species, where selective pressures are very different due to the summer drought imposed over the short growth
season. We hypothesized that late-flowering plants in Mediterranean mountains should present stronger selective pressures
towards early onset of reproduction than early-flowering species, because less water is available in the soil as growing season
progresses. We performed selection analyses on flowering onset and duration in two high mountain species of contrasting phenology.
Since phenotypic selection can be highly context-dependent, we studied several populations of each species for 2 years, covering
their local altitudinal ranges and their different microhabitats. Surrogates of biotic selective agents, like fruitset for
pollinators and flower and fruit loss for flower and seed predators, were included in the analysis. Differences between the
early- and the late-flowering species were less than expected. A consistent negative correlational selection of flowering
onset and duration was found affecting plant fitness, i.e., plants that bloomed earlier flowered for longer periods improving
plant fitness. Nevertheless, the late-flowering species may experience higher risks under climate warming because in extremely
warm and dry years the earlier season does not bring about a longer flowering duration due to summer drought. 相似文献
146.
Eukaryotic genomes are especially vulnerable to DNA damage during the S phase of the cell cycle, when chromosomes must be duplicated. The stability of DNA replication forks is critical to achieve faithful chromosome replication and is severely compromised when forks encounter DNA lesions. To maintain genome integrity, replication forks need to be protected by the S-phase checkpoint and DNA insults must be repaired. Different pathways help to repair or tolerate the lesions in the DNA, but their contribution to the progression of replication forks through damaged DNA is not well known. Here we show in budding yeast that, when the DNA template is damaged with the alkylating agent methyl methanesulfonate (MMS), base excision repair, homologous recombination and DNA damage tolerance pathways, together with a functional S-phase checkpoint, are essential for the efficient progression of DNA replication forks and the maintenance of cell survival. In the absence of base excision repair, replication forks stall reversibly in cells exposed to MMS. This repair reaction is necessary to eliminate the lesions that impede fork progression and has to be coordinated with recombination and damage tolerance activities to avoid fork collapse and allow forks to resume and complete chromosome replication. 相似文献
147.
Aurora Antonio-Pérez Tania Rivera-Hernández Luz María Aldaz-Martínez Jaime Ortega-López 《Biotechnology and Bioprocess Engineering》2012,17(4):703-710
Expression of recombinant proteins in Escherichia coli often leads to formation of inclusion bodies (IB). If a recombinant protein contains one or more disulfide bonds, protein refolding and thiol oxidation reactions are required to recover its biological activity. Previous studies have demonstrated that molecular chaperones and foldases assist with the in vitro protein refolding. However, their use has been limited by the stoichiometric amount required for the refolding reaction. In search of alternatives to facilitate the use of these folding biocatalysts in this study, DsbA, DsbC, and the apical domain of GroEL (AD) were fused to the carbohydrate-binding module CBDCex of Cellulomonas fimi. The recombinant proteins were purified and immobilized in cellulose and used to assist the oxidative refolding of denatured and reduced lysozyme. The assisted refolding yields obtained with immobilized folding biocatalysts were at least twice of those obtained in the spontaneous refolding, suggesting that the AD, DsbA, and DsbC immobilized in cellulose might be useful for the oxidative refolding of recombinant proteins that are expressed as inclusion bodies. In addition, the spontaneous or assisted refolding kinetics data fitted well (r2 > 0.9) to a previously reported lysozyme refolding model. The estimated refolding (k N) and aggregation (k A) constants were consistent with the hypothesis that foldases assisted the oxidative refolding of lysozyme by decreasing protein aggregation rather than increasing the refolding rate. 相似文献
148.
Isolation of plant DNA: A fast,inexpensive, and reliable method 总被引:46,自引:2,他引:46
We describe here a simple method to isolate DNA of high molecular weight from a wide variety of plant materials, such as trees,
herbaceous plants, cell suspension cultures, calli, seeds, dried embryos, ferns and lichens. The crucial step of the extraction
is the use of an acidic extraction medium. When necessary, the sample was separated on a fast RPC-5 column providing us with
highly purified DNA suitable not only for restriction endonuclease analyses but also for PCR experiments, RLFP analyses, or
detection of adducts. 相似文献
149.
150.