全文获取类型
收费全文 | 57412篇 |
免费 | 4250篇 |
国内免费 | 31篇 |
出版年
2023年 | 359篇 |
2022年 | 483篇 |
2021年 | 975篇 |
2020年 | 813篇 |
2019年 | 915篇 |
2018年 | 1512篇 |
2017年 | 1297篇 |
2016年 | 2004篇 |
2015年 | 2874篇 |
2014年 | 2991篇 |
2013年 | 3827篇 |
2012年 | 4924篇 |
2011年 | 4534篇 |
2010年 | 2596篇 |
2009年 | 2194篇 |
2008年 | 3499篇 |
2007年 | 3351篇 |
2006年 | 3136篇 |
2005年 | 2853篇 |
2004年 | 2762篇 |
2003年 | 2556篇 |
2002年 | 2378篇 |
2001年 | 1057篇 |
2000年 | 1116篇 |
1999年 | 751篇 |
1998年 | 439篇 |
1997年 | 305篇 |
1996年 | 310篇 |
1995年 | 307篇 |
1994年 | 301篇 |
1993年 | 257篇 |
1992年 | 344篇 |
1991年 | 271篇 |
1990年 | 244篇 |
1989年 | 239篇 |
1988年 | 206篇 |
1987年 | 179篇 |
1986年 | 147篇 |
1985年 | 183篇 |
1984年 | 207篇 |
1983年 | 137篇 |
1982年 | 156篇 |
1981年 | 128篇 |
1980年 | 105篇 |
1979年 | 128篇 |
1978年 | 112篇 |
1977年 | 95篇 |
1976年 | 119篇 |
1975年 | 105篇 |
1974年 | 99篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
71.
72.
73.
74.
Salomé C. Vilchez Larrea Mariana Schlesinger María L. Kevorkian Mirtha M. Flawiá Guillermo D. Alonso Silvia H. Fernández Villamil 《PloS one》2013,8(6)
Trypanosoma cruzi, etiological agent of Chagas’ disease, has a complex life cycle which involves the invasion of mammalian host cells, differentiation and intracellular replication. Here we report the first insights into the biological role of a poly(ADP-ribose) glycohydrolase in a trypanosomatid (TcPARG). In silico analysis of the TcPARG gene pointed out the conservation of key residues involved in the catalytic process and, by Western blot, we demonstrated that it is expressed in a life stage-dependant manner. Indirect immunofluorescence assays and electron microscopy using an anti-TcPARG antibody showed that this enzyme is localized in the nucleus independently of the presence of DNA damage or cell cycle stage. The addition of poly(ADP-ribose) glycohydrolase inhibitors ADP-HPD (adenosine diphosphate (hydroxymethyl) pyrrolidinediol) or DEA (6,9-diamino-2-ethoxyacridine lactate monohydrate) to the culture media, both at a 1 µM concentration, reduced in vitro epimastigote growth by 35% and 37% respectively, when compared to control cultures. We also showed that ADP-HPD 1 µM can lead to an alteration in the progression of the cell cycle in hydroxyurea synchronized cultures of T. cruzi epimastigotes. Outstandingly, here we demonstrate that the lack of poly(ADP-ribose) glycohydrolase activity in Vero and A549 host cells, achieved by chemical inhibition or iRNA, produces the reduction of the percentage of infected cells as well as the number of amastigotes per cell and trypomastigotes released, leading to a nearly complete abrogation of the infection process. We conclude that both, T. cruzi and the host, poly(ADP-ribose) glycohydrolase activities are important players in the life cycle of Trypanosoma cruzi, emerging as a promising therapeutic target for the treatment of Chagas’ disease. 相似文献
75.
Márcia M. Medeiros Henrique B. da Silva Aramys S. Reis Renato Barboza Joanne Thompson Maria Regina D'Império Lima Cláudio R. F. Marinho Carlos E. Tadokoro 《PloS one》2013,8(11)
It is postulated that accumulation of malaria-infected Red Blood Cells (iRBCs) in the liver could be a parasitic escape mechanism against full destruction by the host immune system. Therefore, we evaluated the in vivo mechanism of this accumulation and its potential immunological consequences. A massive liver accumulation of P. c. chabaudi AS-iRBCs (Pc-iRBCs) was observed by intravital microscopy along with an over expression of ICAM-1 on day 7 of the infection, as measured by qRT-PCR. Phenotypic changes were also observed in regulatory T cells (Tregs) and dendritic cells (DCs) that were isolated from infected livers, which indicate a functional role for Tregs in the regulation of the liver inflammatory immune response. In fact, the suppressive function of liver-Tregs was in vitro tested, which demonstrated the capacity of these cells to suppress naive T cell activation to the same extent as that observed for spleen-Tregs. On the other hand, it is already known that CD4+ T cells isolated from spleens of protozoan parasite-infected mice are refractory to proliferate in vivo. In our experiments, we observed a similar lack of in vitro proliferative capacity in liver CD4+ T cells that were isolated on day 7 of infection. It is also known that nitric oxide and IL-10 are partially involved in acute phase immunosuppression; we found high expression levels of IL-10 and iNOS mRNA in day 7-infected livers, which indicates a possible role for these molecules in the observed immune suppression. Taken together, these results indicate that malaria parasite accumulation within the liver could be an escape mechanism to avoid sterile immunity sponsored by a tolerogenic environment. 相似文献
76.
Roberto Merciai Margarida Casadevall Harold Villegas-Hernández Josep Lloret 《Journal of fish biology》2020,97(2):383-395
Among the thermophilic fishes that have become established in the north-western Mediterranean as a consequence of sea warming, the yellowmouth barracuda (Sphyraena viridensis Cuvier, 1829) appears to be one of the most successful and abundant in the coastal rocky environment, having increasingly become the object of recreational and commercial exploitation in the study area. Lure-fishing sessions were carried out from May 2016 to November 2018 in the Catalan Sea (NE Spain) at dawn and dusk, with the aim of providing new insight into the behavioural, spatial and feeding ecology and vulnerability to angling of this poorly known species. Generalized mixed-effects linear models showed that S. viridensis is a crepuscular inshore dweller, whose vulnerability to angling is significantly influenced by solar and lunar light intensities, being highest in the pre-spawn and spawning periods. Asymmetries between dawn and dusk activity patterns were detected, evidently related to a drop in aggressiveness at dusk following the spawning period. The simple study design adopted may be applied to other contexts, aiming to the recognition of several levels of fish vulnerability to angling. 相似文献
77.
Anatomy of the antennal dorsal organ in female of Neodryinus typhlocybae (Hymenoptera: Dryinidae): A peculiar sensory structure possibly involved in perception of host vibration
下载免费PDF全文
![点击此处可从《Journal of morphology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Paola Riolo Nunzio Isidoro Sara Ruschioni Roxana L. Minuz Ferdinando Bin Roberto Romani 《Journal of morphology》2016,277(1):128-137
Neodryinus typhlocybae (Hymenoptera: Dryinidae) is a natural enemy of the planthopper Metcalfa pruinosa, which was introduced from North America into Europe and has become established in various regions as a pest species. Vibrational signals play a crucial role in the communication of M. pruinosa, which appears to be exploited by N. typhlocybae. Scanning and transmission electron microscopy have shown that the antennae of N. typhlocybae females have peculiar and complex sensory structures: deep longitudinal grooves that house long sensilla trichodea, termed here “Antennal Dorsal Organs.” Such structures were not present on male antennae. These sensilla extend for the length of the grooves, without contact with the groove cuticle. Their hair shaft is empty and aporous, and inserted into a specialized socket, underneath which there is a cuticular ampulla‐like chamber. Each sensillum is associated with two sensory neurons: one terminates at the proximal end of the dendritic sheath; the other continues into the sensillum sinus and is enclosed in the dendritic sheath. This second sensory neuron then enters the ampulla‐like chamber through the circular opening, and then terminates with a conspicuous tubular body at the shaft base. The possible involvement of this peculiar structure in the context of host recognition mechanism is discussed. J. Morphol. 277:128–137, 2016. © 2015 Wiley Periodicals, Inc. 相似文献
78.
Reinhard Told Stefan Palkovits Helmuth Haslacher Sophie Frantal Doreen Schmidl Agnes Boltz Michael Lasta Semira Kaya René M. Werkmeister Gerhard Garh?fer Leopold Schmetterer 《PloS one》2013,8(4)
A common polymorphism in the complement factor H gene (rs1061170, Y402H) is associated with a high risk of age-related macular degeneration (AMD). In the present study we hypothesized that healthy young subjects homozygous for the high-risk haplotype (CC) show abnormal choroidal blood flow (ChBF) regulation decades before potentially developing the disease. A total of 100 healthy young subjects were included in the present study, of which 4 subjects were excluded due to problems with genotyping or blood flow measurements. ChBF was measured continuously using laser Doppler flowmetry while the subjects performed isometric exercise (squatting) for 6 minutes. The increase in ChBF was less pronounced than the response in ocular perfusion pressure (OPP), indicating for some degree of choroidal blood flow regulation. Eighteen subjects were homozygous for C, 47 subjects were homozygous for T and 31 subjects were heterozygous (CT). The increase in OPP during isometric exercise was not different between groups. By contrast the increase in ChBF was more pronounced in subjects homozygous for the high risk C allele (p = 0.041). This was also evident from the pressure/flow relationship, where the increase in ChBF in homozygous C carriers started at lower OPPs as compared to the other groups. Our data indicate that the regulation of ChBF is abnormal in rs1061170 CC carriers. So far this polymorphism has been linked to age related macular degeneration (AMD) mainly via inflammatory pathways associated with the complement system dysfunction. Our results indicate that it could also be related to vascular factors that have been implicated in AMD pathogenesis. 相似文献
79.
Felipe Silva de Andrade Thiago Ribeiro de Carvalho Lucas Borges Martins Ariovaldo Antonio Giaretta 《Studies on Neotropical Fauna and Environment》2017,52(2):157-167
In this study, we re-describe the advertisement and territorial calls of Boana pardalis, carry out an acoustic comparison between the studied species and the other congeners of the B. faber group, and report for the first time the tympanic amplexus for the studied species. The advertisement call of B. pardalis can be used to supplement its diagnosis in the B. faber group based on temporal call traits, e.g. emission rate and emission pattern, as well as the call envelope. 相似文献
80.