全文获取类型
收费全文 | 88947篇 |
免费 | 7196篇 |
国内免费 | 43篇 |
专业分类
96186篇 |
出版年
2023年 | 452篇 |
2022年 | 747篇 |
2021年 | 1403篇 |
2020年 | 1144篇 |
2019年 | 1210篇 |
2018年 | 1976篇 |
2017年 | 1686篇 |
2016年 | 2738篇 |
2015年 | 3968篇 |
2014年 | 3955篇 |
2013年 | 5090篇 |
2012年 | 6447篇 |
2011年 | 6018篇 |
2010年 | 3481篇 |
2009年 | 3087篇 |
2008年 | 4529篇 |
2007年 | 4409篇 |
2006年 | 4181篇 |
2005年 | 4222篇 |
2004年 | 4175篇 |
2003年 | 3656篇 |
2002年 | 3146篇 |
2001年 | 2195篇 |
2000年 | 2191篇 |
1999年 | 1700篇 |
1998年 | 837篇 |
1997年 | 688篇 |
1996年 | 765篇 |
1995年 | 637篇 |
1994年 | 621篇 |
1993年 | 577篇 |
1992年 | 1105篇 |
1991年 | 971篇 |
1990年 | 891篇 |
1989年 | 888篇 |
1988年 | 843篇 |
1987年 | 762篇 |
1986年 | 720篇 |
1985年 | 720篇 |
1984年 | 700篇 |
1983年 | 512篇 |
1982年 | 425篇 |
1981年 | 404篇 |
1980年 | 379篇 |
1979年 | 503篇 |
1978年 | 410篇 |
1977年 | 345篇 |
1975年 | 349篇 |
1974年 | 358篇 |
1973年 | 342篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
971.
Iron deficiency in higher plants causes accumulation of salts of organic acids in the roots, the most characteristic being citrate. We show that citrate and malate accumulate in beans (Phaseolus vulgaris L. var Prélude), not because of a lack of the iron-containing enzyme aconitase (EC 4.2.1.3), but in close coupling to the extrusion of protons during rhizosphere acidification, one of the `Fe-efficiency' reactions of dicotyledonous plants. When proton excretion is induced in roots of control bean plants by addition of fusicoccin, only malate, not citrate, is accumulated. We propose that iron deficiency induces production of organic acids in the roots, which in beans leads to both proton excretion and an increased capacity to reduce ferric chelates via the induced electron transfer system in the root epidermis cells. 相似文献
972.
Summary. The aim of this work was to study the activity of NAD(P)H:(quinone acceptor) oxidoreductase 1 (EC 1.6.99.2) in the regeneration
of lipophilic antioxidants, alpha-tocopherol, and reduced-coenzyme Q analogs. First, we tested whether or not two isoforms
of the NAD(P)H:(quinone acceptor) oxidoreductase 1 designated as “hydrophilic” and “hydrophobic” (H. J. Prochaska and P. Talalay,
Journal of Biological Chemistry 261: 1372–1378, 1986) show differential enzyme activities towards hydrophilic or hydrophobic
ubiquinone homologs. By chromatography on phenyl Sepharose, we purified the two isoforms from pig liver cytosol and measured
their reduction of several ubiquinone homologs of different side chain length. We also studied by electron paramagnetic resonance
the effect of NAD(P)H:(quinone acceptor) oxidoreductase 1 on steady-state levels of chromanoxyl radicals generated by linoleic
acid and lipooxygenase and confirmed the enzyme's ability to protect alpha-tocopherol against oxidation induced with H2O2-Fe2+. Our results demonstrated that the different hydrophobicities of the isoforms do not reflect different reactivities towards
ubiquinones of different side chain length. In addition, electron paramagnetic resonance studies showed that in systems containing
the reductase plus NADH, levels of chromanoxyl radicals were dramatically reduced. Morever, in the presence of oxidants, alpha-tocopherol
was preserved by NAD(P)H:(quinone acceptor) oxidoreductase 1, supporting our hypothesis that regeneration of alpha-tocopherol
may be one of the physiologic functions of this enzyme.
Received May 20, 2002; accepted September 20, 2002; published online May 21, 2003
RID="*"
ID="*" Correspondence and reprints: Departamento de Biología Celular, Fisiología e Inmunología, Facultad de Ciencias, Edificio
Severo Ochoa, Campus de Rabanales, Universidad de Córdoba, 14014 Córdoba, Spain. 相似文献
973.
Francisco G. Acién Fernández Celeste Brindley Alias José A. Sánchez Pérez José M. Fernández Sevilla María J. Ibáñez González Emilio Molina Grima 《Journal of applied phycology》2003,15(2-3):229-237
An integrated process for the indoor production of 13C labelled PUFA from Phaeodactylum tricornutum is presented. The core of the process is a bubble column photobioreactor from which the exhaust gas from the reactor is returned to the culture by a low pressure compressor. To avoid accumulation of dissolved oxygen in the culture medium, the exhaust gas is bubbled through a sodium sulphite solution before returning it to the reactor. Carbon is removed from the medium before inoculating the alga, then labelled 13CO2 is injected for pH control and carbon supply. The reactor has been operated in semicontinuous mode at a dilution rate of 0.01 h–1, a biomass productivity of 0.1 g L–1 d–1 being obtained. Under this conditions both pH and dissolved oxygen were correctly controlled and the adequacy of the system for autotrophic production of labelled biomass was demonstrated. Analysis by GC-MS revealed that the fatty acids content of the biomass obtained was 10% d.wt., the content of eicosapentaenoic acid was 2.5% d.wt. All the fatty acids were labelled, more that 90% of the carbon present in these fatty acids was 13C. Element analysis of biomass and supernatant showed that 59.5% of injected carbon was assimilated into the biomass whereas 33% remained in the supernatant, and 7.5% remained undetected. Due to the high cost of 13CO2 different strategies for the optimisation of labelled carbon use are proposed. 相似文献
974.
975.
Spatial patterns of trees and juveniles in a laurel forest of Tenerife, Canary Islands 总被引:5,自引:0,他引:5
Spatial patterns are important characteristics of the forest and theycan reveal such things as successional status and ecological characteristics ofthe species. We tested the hypothesis that spatial distribution will bedifferent, depending on whether the species is intolerant or tolerant to shade.We assessed the spatial distribution of trees (> 4 cm dbh) andjuveniles in eight laurel forest plots. A univariate spatial analysis(performed with Ripley's K1) showed that all tree species havesignificantaggregation at short distances (2 m). Nevertheless, two groups ofspecies could be differentiated: Erica scoparia,Myrica faya and Ilex canariensisshowed a tendency for aggregation at large distances (larger than 6m)while L. azorica and Prunuslusitanicashowed aggregation only at shorter distances. Ripley's BivariateK1,2 analyses showed no significant differences in the spatialdistribution ofanalyzed species pairs from a null model. Only Laurusazoricahad a sufficient sample size for analysis of juvenile distribution. Aunivariateanalysis revealed thatL. azorica seedlings (stems < 50 cm high)were clumped in some plots up to 5 m, but this was not consistent.Saplings (stems > 50 cm high and < 4 cm dbh)didnot show strong clumping even at short distances. L.azoricasaplings had no significant aggregation with, nor repulsionfrom, adults of the same or different species. Spatial patterns of the speciesshould be considered in the development of restoration plans of the laurelforest 90%of which has disappeared or been intensively disturbed on Tenerife Island. 相似文献
976.
The construction of large reservoirs in South America and particularly in Brazil has intensified within the last 50 years. Built up primarily for hydroelectricity production, these artificial ecosystems now serve purposes such as: water storage for public use; fisheries and aquaculture; recreation; tourism, and irrigation. These artificial ecosystems were also built up with the purpose of enhancing the regional development. These activities produce multiple impacts among which are eutrophication, a serious problems with various ecological, economic, and social consequences. Basic studies on reservoirs have identified their main ecological characteristics and described some of their fundamental mechanisms of functioning. This information cover: factors involved in reservoir complexity, such as spatial scale, vertical and horizontal heterogeneity, temporal variation at several time scales and, in some cases, watershed/reservoir relationships, and the impact of non-point and point sources of nutrients. Numerous studies have been dedicated to describing aquatic biota, its relationship with environmental factors, and the impacts of degradation/pollution/ eutrophication on biodiversity. Research priorities have been established mainly by limnologists in response to needs identified by environmental impact assessment. Due to the pressure of multiple uses, population growth, and economic factors, aquatic scientists now face a number of questions being posed by managers and engineers. These questions relate to a wide range of practical problems, the solutions to which depend on accumulated data related to the structural complexities and function mechanisms previously referred to. These problems can be divided into three main groups: (i) eutrophication processes and their characteristics, and water quality control; (ii) impact of fisheries, aquaculture, and exotic species introduction on the biota and the water quality; (iii) sustainable development of reservoirs and optimization management of their multiple uses. Predictive capabilities required in the face of these problems should be based on modeling development and intensive use of data-bases resulting from long-term studies on reservoirs. The predictions thus made possible would involve economic factors and the costs of recovery of eutrophic reservoirs. The experience of reservoir research and management in South America and in Brazil is, probably part of a world wide tendency on reservoir research and development with the aim to optimize multiple uses. Based on the cooperation of limnologists and engineers, ecosystem management models must eventually be applied at the watershed / reservoir level, where demands on the part of managers and decision makers will certainly promote a predictive, integrated, and adaptive approach,based on fundamental research. 相似文献
977.
Evidence suggests that humans might have neurological specializations for music processing, but a compelling adaptationist
account of music and dance is lacking. The sexual selection hypothesis cannot easily account for the widespread performance
of music and dance in groups (especially synchronized performances), and the social bonding hypothesis has severe theoretical
difficulties. Humans are unique among the primates in their ability to form cooperative alliances between groups in the absence
of consanguineal ties. We propose that this unique form of social organization is predicated on music and dance. Music and
dance may have evolved as a coalition signaling system that could, among other things, credibly communicate coalition quality,
thus permitting meaningful cooperative relationships between groups. This capability may have evolved from coordinated territorial
defense signals that are common in many social species, including chimpanzees. We present a study in which manipulation of
music synchrony significantly altered subjects’ perceptions of music quality, and in which subjects’ perceptions of music
quality were correlated with their perceptions of coalition quality, supporting our hypothesis. Our hypothesis also has implications
for the evolution of psychological mechanisms underlying cultural production in other domains such as food preparation, clothing
and body decoration, storytelling and ritual, and tools and other artifacts.
Edward Hagen is a research scientist at the Institute for Theoretical Biology, Humboldt University, Berlin. Gregory Bryant
is a doctoral candidate in the Department of Psychology, University of California, Santa Cruz. 相似文献
978.
979.
Vasoactive intestinal peptide (VIP) and pituitary adenylate cyclase-activating polypeptide (PACAP) are important neuropeptides in the control of lung physiology. Both of these commonly bind to specific G protein coupled receptors named VPAC(1)-R and VPAC(2)-R, and PAC(1)-R (with higher affinity for PACAP). VIP and PACAP have been implicated in the control of cell proliferation and tumor growth. This study examined the presence of VIP and PACAP receptors in human lung cancer samples, as well as the functionality of adenylyl cyclase (AC) stimulated by both peptides. Results from RT-PCR and immunoblot experiments showed the expression of VPAC(1)-, VPAC(2)- and PAC(1)-R in lung cancer samples. Immunohistochemical studies showed the expression of VPAC(1) and VPAC(2) receptors. These receptors were positively coupled to AC, but the enzyme activity was impaired as compared to normal lung. There were no changes in Galpha(s) or Galpha(i) levels. Present results contribute to a better knowledge of VIP/PACAP actions in lung cancer and support the interest for the development of VIP/PACAP analogues with therapeutic roles. 相似文献
980.
Semedo T Santos MA Lopes MF Figueiredo Marques JJ Barreto Crespo MT Tenreiro R 《Systematic and applied microbiology》2003,26(1):13-22
The occurrence of several virulence traits (cytolysin, adhesins and hydrolytic enzymes) was investigated in a collection of 164 enterococci, including food and clinical isolates (from human and veterinary origin), as well as type and reference strains from 20 enterococcal species. Up to fifteen different cyl genotypes were found, as well as silent cyl genes. The occurrence of the cyl operon and haemolytic potential seems to be widespread in the genus. A significant association of this virulent trait with clinical isolates was found (p < 0.05). High levels of incidence were also observed for genes encoding surface adhesins (esp, efaA(fs), efaA(fm)), agg and gelE, irrespectively of species allocation and origin of strains. Although gelE behaves as silent in the majority of the strains, gelatinase activity predominates in clinical isolates, whereas lipase and DNase were mainly detected in food isolates pointing to their minor role as virulence determinants. No hyaluronidase activity was detected for all strains. Numerical hierarchic data analysis grouped the strains in three main clusters, two of them including a total of 50 strains with low number of virulence determinants (from 2 to 7) and the other with 114 strains with a high virulence potential (up to 12 determinants). No statistical association was found between virulence clusters and species allocation (p > 0.10), strongly suggesting that virulence determinants are a common trait in the genus Enterococcus. Clinical strains seem to be significantly associated with high virulence potential, whereas food, commensal and environmental strains harbour fewer virulence determinants (p < 0.01). A high level of relative diversity in virulence patterns was observed (Shannon's index varies from 0.95 to 1.0 among clusters), reinforcing the strain-specific nature of the association of virulence factors. Although a low risk seems to be associated with the use of enterococci in long-established artisanal cheeses, screening of virulence traits and their cross-synergies must be performed, particularly for commercial starters, probiotic strains and products to be used by high risk population groups. 相似文献