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951.
952.
André Lasalle Walter Norbis Denise Vizziano-Cantonnet 《Zeitschrift fur angewandte Ichthyologie》2021,37(6):835-846
The purpose of this study was to detail a simple strategy for sexing morphologically-undifferentiated fish using statistical analysis of gene expression patterns characterized by quantitative PCR. This approach is especially relevant for species without known genomic sex markers. The method was developed for early identification of female Siberian sturgeon as part of a genomics study. That study documented activation of the enzyme 17ß-hydroxy-steroid-dehydrogenase (hsd17b1) in future ovarian tissue at 3 months of age, concurrent with a small forkhead box L2 (foxl2) peak and emerging cytochrome P450, family 19, subfamily A (cyp19a1) expression. Major cyp19a1 and foxl2 peaks occurred in presumptive female gonads at 5–6 months. This pattern suggested a genetic relay mediating estrogen production throughout differentiation, possibly to maintain gonadal femininity. Genes involved in stem cell proliferation (lim homeobox 2 (lhx2)) and somatic-germ cell interaction maintenance (iroquois homeobox 5 (irx5) and iroquois homeobox 3 (irx3)) were also expressed during molecular differentiation, at 5–6 months. The roles of lhx2, irx3, and irx5 in fish sex differentiation should be confirmed using other methodologies. These results indicate that estrogens are crucial for ovarian differentiation in basal non-teleost fish, consistent with well-established patterns in teleosts, with hsd17b1 as one of the earliest biomarkers of gonadal development. 相似文献
953.
Berti Francesco Romano Maria Rosaria Micoli Francesca Adamo Roberto 《Glycoconjugate journal》2021,38(4):401-409
Glycoconjugate Journal - Neisseria meningitidis is a major cause of bacterial meningitidis worldwide. Children less than five years and adolescents are particularly affected. Nearly all invasive... 相似文献
954.
Philippou Anastassios Tryfonos Andrea Theos Apostolos Nezos Adrianos Halapas Antonis Maridaki Maria Koutsilieris Michael 《Molecular biology reports》2021,48(5):4047-4054
Molecular Biology Reports - Eccentric exercise has been extensively used as a model to study the contraction-induced muscle damage and its consequent processes. This study aimed at examining... 相似文献
955.
Joseph Tibbs Mohamed Ghoneim Colleen C Caldwell Troy Buzynski Wayne Bowie Elizabeth
M Boehm M
Todd Washington S
M
Ali Tabei Maria Spies 《Nucleic acids research》2021,49(9):e53
Molecular machines within cells dynamically assemble, disassemble and reorganize. Molecular interactions between their components can be observed at the single-molecule level and quantified using colocalization single-molecule spectroscopy, in which individual labeled molecules are seen transiently associating with a surface-tethered partner, or other total internal reflection fluorescence microscopy approaches in which the interactions elicit changes in fluorescence in the labeled surface-tethered partner. When multiple interacting partners can form ternary, quaternary and higher order complexes, the types of spatial and temporal organization of these complexes can be deduced from the order of appearance and reorganization of the components. Time evolution of complex architectures can be followed by changes in the fluorescence behavior in multiple channels. Here, we describe the kinetic event resolving algorithm (KERA), a software tool for organizing and sorting the discretized fluorescent trajectories from a range of single-molecule experiments. KERA organizes the data in groups by transition patterns, and displays exhaustive dwell time data for each interaction sequence. Enumerating and quantifying sequences of molecular interactions provides important information regarding the underlying mechanism of the assembly, dynamics and architecture of the macromolecular complexes. We demonstrate KERA’s utility by analyzing conformational dynamics of two DNA binding proteins: replication protein A and xeroderma pigmentosum complementation group D helicase. 相似文献
956.
Morales-Gamba Ruben Dario de Araújo Maria Lúcia Góes Barcellos Jose Fernando Marcon Jaydione Luiz 《Environmental Biology of Fishes》2021,104(12):1665-1672
Environmental Biology of Fishes - This study evaluated how the plasma steroid hormones testosterone (T) and 17β-estradiol (E2) are related to follicular development in regenerating females of... 相似文献
957.
Meno Laura Escuredo Olga Rodríguez-Flores Maria Shantal Seijo Maria Carmen 《Aerobiologia》2021,37(2):309-320
Aerobiologia - Potatoes production is the main source of income in A Limia (Northwest Spain). Most of the potatoes harvest is stored in piles in farmer´s warehouses without systems to control... 相似文献
958.
Feldmane Daina Druva-Lūsīte Ieva Pole Valentīna Butac Mădălina Maria Militaru Mădălina Missa Imants Meiere Diāna Rubauskis Edgars 《Journal of Plant Growth Regulation》2021,40(2):533-540
Journal of Plant Growth Regulation - Sour cherry ‘Latvijas Zemais’ (Prunus cerasus) is a promising dwarfing rootstock for sweet cherries in Latvia, but low growing rate of newly... 相似文献
959.
Terzin Marko Paletta Maria Grazia Matterson Kenan Coppari Martina Bavestrello Giorgio Abbiati Marco Bo Marzia Costantini Federica 《Coral reefs (Online)》2021,40(4):1391-1391
Coral Reefs - A correction to this paper has been published: https://doi.org/10.1007/s00338-021-02120-y 相似文献
960.
da Silva Fernando Henrique Alves de Morais Patrícia Lígia Dantas da Silva Dias Nildo de Sousa Nunes Glauber Henrique de Morais Marciana Bizerra Melo Marlenildo Ferreira de Albuquerque Nascimento Maria Tereza 《Journal of Plant Growth Regulation》2021,40(3):1298-1314
Journal of Plant Growth Regulation - This study evaluated the effect of saline water irrigation (4.5 dS m−1) on growth, gas exchange and mineral nutrient content in eight melon accessions and... 相似文献