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761.
762.
Don A. Driscoll Annabel L. Smith Samantha Blight John Maindonald 《Biodiversity and Conservation》2012,21(6):1607-1625
Altered fire regimes are a driver of biodiversity decline. To plan effective management, we need to know how species are influenced
by fire and to develop theory describing fire responses. Animal responses to fire are usually measured using methods that
rely on animal activity, but animal activity may vary with time since fire, potentially biasing results. Using a novel approach
for detecting bias in the pit-fall trap method, we found that leaf-litter dependent reptiles were more active up to 6 weeks
after fire, giving a misleading impression of abundance. This effect was not discovered when modelling detectability with
zero-inflated binomial models. Two species without detection bias showed early-successional responses to time since fire,
consistent with a habitat-accommodation succession model. However, a habitat specialist did not have the predicted low abundance
after fire due to increased post-fire movement and non-linear recovery of a key habitat component. Interactions between fire
and other processes therefore must be better understood to predict reptile responses to changing fire-regimes. We conclude
that there is substantial bias when trapping reptiles after fire, with species that are otherwise hard to detect appearing
to be abundant. Studies that use a survey method based on animal activity such as bird calls or animal movements, likely face
a similar risk of bias when comparing recently-disturbed with control sites. 相似文献
763.
Stop codon recognition in ciliates: Euplotes release factor does not respond to reassigned UGA codon 总被引:5,自引:0,他引:5
In eukaryotes, the polypeptide release factor 1 (eRF1) is involved in translation termination at all three stop codons. However, the mechanism for decoding stop codons remains unknown. A direct interaction of eRF1 with the stop codons has been postulated. Recent studies focus on eRF1 from ciliates in which some stop codons are reassigned to sense codons. Using an in vitro assay based on mammalian ribosomes, we show that eRF1 from the ciliate Euplotes aediculatus responds to UAA and UAG as stop codons and lacks the capacity to decipher the UGA codon, which encodes cysteine in this organism. This result strongly suggests that in ciliates with variant genetic codes eRF1 does not recognize the reassigned codons. Recent hypotheses describing stop codon discrimination by eRF1 are not fully consistent with the set of eRF1 sequences available so far and require direct experimental testing. 相似文献
764.
Little information exists on mixed-species groups between primates and other mammals in Neotropical forests. In this paper, we describe three such associations observed during an extensive large-vertebrate survey in central Amazonia, Brazil. Mixed-species groups between a primate species and another mammal were observed on seven occasions between squirrel monkeys (Saimiri cf. ustus) and either South American coatis (Nasua nasua) or tayras (Eira barbara) and between brown capuchins (Cebus apella) and coatis. All associations were restricted to floodplain forest during its dry stage. We suggest that the associations involving the coatis are connected to foraging and vigilance but may be induced by a common alternative food resource at a time of food shortage. 相似文献
765.
Species diversity of the genus Osmundea (Ceramiales,Rhodophyta) in the Macaronesian region 下载免费PDF全文
María Machín‐Sánchez Florence Rousseau Line Le Gall Valéria Cassano Ana I. Neto Abel Sentíes Mutue T. Fujii María Candelaria Gil‐Rodríguez 《Journal of phycology》2016,52(4):664-681
Species diversity within the genus Osmundea in the Macaronesian region was explored by conducting a comprehensive sampling in the Azores, the Canary, and the Madeira archipelagos. Toward identification, all specimens were first observed alive to verify the absence of corps en cerise, a diagnostic character for the genus and morphometric data were measured (thallus length and width, first‐order branches length and width, branchlets length and width, cortical cell length and width in surface view, cortical cell length and width in transverse section). Specimens were sequenced for COI‐5P (39 specimens) and three species delimitation methods (Generalized Mixed Yule Coalescent, Automatic Barcode Gap Discovery method, and Poisson Tree Processes) were used to assess the threshold between infra‐ and interspecific relationships. Subsequently, one or several sequences of plastid‐encoded large subunit of RuBisCO (21 specimens) per delimited species were generated to assess the phylogenetic relationships among Macaronesian Osmundea. Moreover, for each delineated species, vegetative and reproductive anatomy was thoroughly documented and, when possible, specimens were either assigned to existing taxa or described as novel species. This integrative approach has provided data for (i) the presence of O. oederi, O. pinnatifida, and O. truncata in Macaronesia; (ii) the proposal of two novel species, O. prudhommevanreinei sp. nov. and O. silvae sp. nov.; and (iii) evidence of an additional species referred as “Osmundea sp.1,” which is a sister taxon of O. hybrida. 相似文献
766.
Lentiviral vectors have been used for gene transfer into the liver but their ability to efficiently transduce quiescent hepatocytes
remains controversial. Lentivirus-mediated gene transfer is more efficient in cycling cells. We determine the effect of H-IL6
in the lentiviral transduction. The lentiviral vector was used to transduce HepG2 cells and mice liver cells, previously treated
with H-IL6. The highest transduction level was observed in HepG2 cells treated with 30 ng/mL H-IL6 and in the mice that received
4 μg H-IL6. Our results suggest that H-IL6 is an inducer of lentiviral gene transfer into the liver cells without any toxicity. 相似文献
767.
Immunohistochemical distribution of inter-alpha-trypsin inhibitor chains in normal and malignant human lung tissue. 总被引:2,自引:0,他引:2
J Bourguignon H Borghi R Sesboüé M Diarra-Mehrpour J F Bernaudin J Métayer J P Martin L Thiberville 《The journal of histochemistry and cytochemistry》1999,47(12):1625-1632
The inter-alpha-trypsin inhibitor (ITI) family is a group of plasma proteins built up from heavy (HC1, HC2, HC3) and light (bikunin) chains synthesized in the liver. In this study we determined the distribution of ITI constitutive chains in normal and cancerous lung tissues using polyclonal antibodies. In normal lung tissue, H2, H3, and bikunin chains were found in polymorphonuclear cells, whereas H1 and bikunin proteins were found in mast cells. Bikunin was further observed in bronchoepithelial mucous cells. In lung carcinoma, similar findings were obtained on infiltrating polymorphonuclear and mast cells surrounding the tumor islets. Highly differentiated cancerous cells displayed strong intracytoplasmic staining with H1 and bikunin antiserum in both adenocarcinoma and squamous cell carcinoma. Moreover, weak but frequent H2 expression was observed in adenocarcinoma cells, whereas no H3-related protein could be detected in cancer cells. Local lung ITI expression was confirmed by RT-PCR. Although the respective role of inflammatory and tumor cells in ITI chain synthesis cannot be presently clarified, these results show that heavy chains as well as bikunin are involved in malignant transformation of lung tissue.(J Histochem Cytochem 47:1625-1632, 1999) 相似文献
768.
Recruitment is a complex process consisting of sequential stages affected by biotic interactions and abiotic factors. Assessment of these sequential stages and corresponding subprocesses may be useful in identifying the most critical stages. Accordingly, to assess the factors that may determine the altitudinal range limits of the high mountain Mediterranean plant Silene ciliata, a set of demographic stages, from flower production to establishment of 2‐yr‐old plants, and their influence on recruitment probability were examined using a step‐by‐step approach. We integrated florivory, pollination and pre‐dispersal seed predation as pre‐dispersal factors, and seedling emergence and survival as post‐dispersal determinants of recruitment. Three populations were monitored at the southernmost margin of the species along its local altitudinal range. Previous studies suggest that seediness is strongly limited by summer drought especially at the lower boundary of the species, a situation that may worsen under current global warming. Our results showed that recruitment was mainly limited by low seed production in the pre‐dispersal stage and low seedling emergence and survival in the post‐dispersal stage, probably due to environmental harshness in summer. By contrast, biotic factors responsible for propagule loss, such as flower and fruit predation, had a minor effect on the probability of plant recruitment. Although the relative importance of transition probabilities was similar among populations along the altitudinal range, comparatively lower flower production significantly reduced the number of recruited plants at the lowest altitude population. This demographic bottleneck, together with increased competition with other species favoured by climate warming, might collapse population growth and limit persistence at the lower altitudinal range of the species, raising its low local altitudinal edge. 相似文献
769.
Thomas Proft 《Biotechnology letters》2010,32(1):1-10
Sortases are transpeptidases produced by Gram-positive bacteria to anchor cell surface proteins covalently to the cell wall.
The Staphylococcus aureus sortase A (SrtA) cleaves a short C-terminal recognition motif (LPXTG) on the target protein followed by the formation of an amide bond with the pentaglycine
cross-bridge in the cell wall. Over recent years, several researchers have exploited this specific reaction for a range of
biotechnology applications, including the incorporation of non-native peptides and non-peptidic molecules into proteins, the
generation of nucleic acid–peptide conjugates and neoglycoconjugates, protein circularisation, and labelling of cell surface
proteins on living cells. 相似文献
770.
Seedlings of two barley genotypes (‘Maresi’ and wild form of Hordeum spontaneum) were treated with jasmonic acid (JA 5 μM and 15 μM) for 24 h, and then subjected to water stress (PEG 6000 solution of −
1.5 MPa). JA caused an increase in the content of ABA but not in that of proline and spermidine in the two studied genotypes.
The effect of the treatment did not depend on the applied JA concentration. The pre-stress treatment with JA changed plant
response to water deficit with regard to membrane injury. Treatment with a lower JA concentration (5 μM) caused a substantial
reduction of the stress-induced membrane damage in the both genotypes. A higher JA concentration (15 μM) caused the reduction
of membrane injury only in H. spontaneum and was ineffective in ‘Maresi’. JA had no influence on the leaf water status in water-stressed plants. A possible role of
JA in leaf ABA accumulation and alleviation of cell membrane injury under water deficit is discussed.
The work was partly supported by the Polish Committee For Scientific Research, grant No 5 PO6A 036 18 相似文献