全文获取类型
收费全文 | 25375篇 |
免费 | 1780篇 |
国内免费 | 29篇 |
专业分类
27184篇 |
出版年
2023年 | 166篇 |
2022年 | 190篇 |
2021年 | 342篇 |
2020年 | 354篇 |
2019年 | 341篇 |
2018年 | 751篇 |
2017年 | 636篇 |
2016年 | 953篇 |
2015年 | 1375篇 |
2014年 | 1390篇 |
2013年 | 1856篇 |
2012年 | 2179篇 |
2011年 | 2036篇 |
2010年 | 1220篇 |
2009年 | 1013篇 |
2008年 | 1502篇 |
2007年 | 1466篇 |
2006年 | 1368篇 |
2005年 | 1229篇 |
2004年 | 1226篇 |
2003年 | 1086篇 |
2002年 | 1041篇 |
2001年 | 255篇 |
2000年 | 215篇 |
1999年 | 239篇 |
1998年 | 190篇 |
1997年 | 135篇 |
1996年 | 136篇 |
1995年 | 155篇 |
1994年 | 139篇 |
1993年 | 120篇 |
1992年 | 151篇 |
1991年 | 120篇 |
1990年 | 117篇 |
1989年 | 102篇 |
1988年 | 101篇 |
1987年 | 80篇 |
1986年 | 60篇 |
1985年 | 86篇 |
1984年 | 82篇 |
1983年 | 69篇 |
1982年 | 74篇 |
1981年 | 60篇 |
1980年 | 41篇 |
1979年 | 58篇 |
1978年 | 51篇 |
1977年 | 43篇 |
1976年 | 53篇 |
1975年 | 45篇 |
1974年 | 48篇 |
排序方式: 共有10000条查询结果,搜索用时 8 毫秒
61.
A crocodilian skeleton from the «série du gypse d'Aix (basal Aquitanian) at Venelles (Bouches-du-Rhône) is described and referred to Diplocynodon cf. rateli. The specimen seems to have been mutilated by scavengers before burial. The occurrence of the freshwater crocodilian Diplocynodon in the «série du gypse d'Aix is in agreement with recent reconstructions of the depositional environment, which suggest a basin with a fluctuating, but usually low, salinity. 相似文献
62.
Résumé Pour atteindre les buts visés par le recyclage biologique des eaux usées, il faut exploiter une population d'organismes filtreurs dont l'augmentation nette de biomasse est rapide et importante. L'augmentation nette de biomasse sera favorisée par une croissance individuelle, une reproduction et une survie excellentes dans l'eau usée traitée. L'étude de ces paramèters sur des cohortes de Daphnia magna soumises à différentes conditions expérimentales, indique qu'il s'agit d'un organisme prometteur pour le recyclage biologique. Dans nos conditions expérimentales, D. magna montre un bilan azoté positif durant toute sa vie. La production rapide de biomasse est favorisée par une température élevée (15 °C) et une nourriture abondante. L'eau traité montre les signes d'une certaine toxicité envers les juvéniles mais non envers les adultes. Nos données établissent que la production d'oeufs requiert une taille minimale de la femelle de 2,7 mm, information primordiale pour l'exploitation future de la population.
To realize the objectives of the biological recycling of wastewaters, it is necessary to exploit a population of filtering organisms, the biomass of which shows a rapid and important net increase. The net increase of biomass is favored by excellent individual growth, reproduction and survival in treated wastewaters. The study of these parameters, with cohorts of Daphnia magna submitted to different treatments, indicated that it is a promising organism for biological recycling. Under our experimental conditions, D. magna exhibited a positive nitrogen balance for the whole of its life. The rapid production of biomass was favored by high temperature (15 °C) and abundant food. The treated wastewaters proved to be somewhat toxic to juveniles but not to adults. Our results demonstrated that the egg production requires a female minimal size of 2.7 mm; this information is most important in the future exploitation of the population.相似文献
63.
The effects of regeneration on ecdysteroid levels and duration of the 10th larval instar of female house crickets were studied. Three experimental groups were used. The first consisted of larvae regenerating two legs cut off on the first day of this stage. The second group was made up of insects that underwent either an epidermal incision (sham-operated group I) or bleeding (sham-operated group II) at the same time as amputation in the first group. The last group was composed of normal insects.Larval stage length: in the first two groups, the duration of the 10th larval stage was significantly modified. It increased for regenerating animals as well as for sham-operated group I, but decreased for sham-operated group II.Ecdysteroid production: controls showed two periods of intense ecdysteroid activity. The first, which took place between days 4 and 6, was assumed to induce apolysis of the tegument and to render regeneration impossible (it coincided with the so-called critical period for regeneration). The second, which occurred between days 7 and 8 initiated cuticle synthesis. For sham-operated insects neither epidermal injury nor a loss of blood appear to change this hormonal profile significantly. Regenerating crickets however exhibited drastically reduced ecdysteroid peaks: the total apparent production of ecdysteriods was 50% below normal. Moreover, relative concentration of ecdysone to 20-hydroxy-ecdysone was greatly increased. Thus, regeneration obviously has a great and specific influence on ecdysteroid release in insect larvae. This effect may be related to changes observed in the prothoracic gland cycle which suggest that it plays a complex role in the regulation of the regenerative process. 相似文献
64.
JoséLuis Avila Antonio Bretaña María Argelia Casanova Angela Avila Francisco Rodríguez 《Experimental parasitology》1979,48(1):27-35
A liquid medium was developed for the continuous cultivation of Trypanosoma cruzi. Among the several highly purified macromolecules tested only bovine liver catalase, horseradish peroxidase, lactoperoxidase, and bovine hemoglobin supported the continuous growth, at high yield, of mice-virulent Trypanosoma cruzi; other hemoproteins were inactive. Bovine liver catalase showed optimal Trypanosoma cruzi growth-promoting activity, parasites reaching 20 × 106 parasites/ml (95% epimastigotes) at about 10 days in most of the 45 subpassages to date. Furthermore, this protein in the incubation medium provided all the amino acid requirements of actively growing parasites, thus eliminating the need for exogeneous free amino acids. Additional experiments revealed that the hemoprotein's growth-promoting activity was independent of any enzymatic activity and that reconstituting the exact protein composition by means of exogeneous amino acids did not support parasite multiplication, suggesting the importance of the primary structure of the active proteins for growth-promoting activity. These active macromolecules supported the multiplication of five different strains of Trypanosoma cruzi, but did not support Leishmania brasiliensis or Leishmania mexicana proliferation, suggesting species specificity. 相似文献
65.
Synchronous growth of a freshwater diatom Melosira italica under natural environment 总被引:1,自引:0,他引:1
Summary A filamentous diatom Melosira italica was collected at the beginning of rainy season from a shallow lake in the tropical savanna region in Brazil. Even the sample taken from surface water contained empty cells in high percentages. The number of cells per filament of M. italica showed a peculiar pulse-like frequency distribution with peak values at 4, 8, 12 and 16. Evidences of the synchronous cell division in this planktonic diatom under natural environment are discussed. 相似文献
66.
67.
68.
Prof. Dr. Dr. R. Wideröe 《Radiation and environmental biophysics》1975,12(3):233-240
Oxygen concentration will influence the alpha- and beta-effect (two-component theory of radiation effects) independently. The beta-effect is reduced by a dose-modifying factor gamma. Oxygen, in competition with enzyme repair actions fortifies a part of the beta-lesions and this model leads to a simple equation for the factor gamma. The alpha-effect is also enhanced by oxygen. Measurements of OER indicate that the reduction of the effect from aerobic to anoxic condition might be about 70%. The functional dependence of oxygen concentration has not been investigated. For small oxygen concentrations of 0.15 to 0.5 muM/l and doses below 1000 R Révész and Littbrand have found that oxygen can protect the irradiated cells and thus increase survivals with about 10%. This is explained as a scavenger action where radical hydrogen atoms are oxidized and hydrated electrons captured by oxygen moelcules. When the oxygen concentration is increased, or with higher doses, the usual sensitizing effect of oxygen exceeds the protection effect. The influence of oxygen on alpha-effects are mainly connected with indirect radiation effects and thus depend on temperature and milieu. 相似文献
69.
Katherine M. Littlefield Rene O. Watson Jennifer M. Schneider Charles P. Neff Eiko Yamada Min Zhang Thomas B. Campbell Michael T. Falta Sarah E. Jolley Andrew P. Fontenot Brent E. Palmer 《PLoS pathogens》2022,18(5)
As of January 2022, at least 60 million individuals are estimated to develop post-acute sequelae of SARS-CoV-2 (PASC) after infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). While elevated levels of SARS-CoV-2-specific T cells have been observed in non-specific PASC, little is known about their impact on pulmonary function which is compromised in the majority of these individuals. This study compares frequencies of SARS-CoV-2-specific T cells and inflammatory markers with lung function in participants with pulmonary PASC and resolved COVID-19 (RC). Compared to RC, participants with respiratory PASC had between 6- and 105-fold higher frequencies of IFN-γ- and TNF-α-producing SARS-CoV-2-specific CD4+ and CD8+ T cells in peripheral blood, and elevated levels of plasma CRP and IL-6. Importantly, in PASC participants the frequency of TNF-α-producing SARS-CoV-2-specific CD4+ and CD8+ T cells, which exhibited the highest levels of Ki67 indicating they were activity dividing, correlated positively with plasma IL-6 and negatively with measures of lung function, including forced expiratory volume in one second (FEV1), while increased frequencies of IFN-γ-producing SARS-CoV-2-specific T cells associated with prolonged dyspnea. Statistical analyses stratified by age, number of comorbidities and hospitalization status demonstrated that none of these factors affect differences in the frequency of SARS-CoV-2 T cells and plasma IL-6 levels measured between PASC and RC cohorts. Taken together, these findings demonstrate elevated frequencies of SARS-CoV-2-specific T cells in individuals with pulmonary PASC are associated with increased systemic inflammation and decreased lung function, suggesting that SARS-CoV-2-specific T cells contribute to lingering pulmonary symptoms. These findings also provide mechanistic insight on the pathophysiology of PASC that can inform development of potential treatments to reduce symptom burden. 相似文献
70.
James F. Saracco Rene L. Cormier Diana L. Humple Sarah Stock Ron Taylor Rodney B. Siegel 《Ecology and evolution》2022,12(6)
The demography and dynamics of migratory bird populations depend on patterns of movement and habitat quality across the annual cycle. We leveraged archival GPS‐tagging data, climate data, remote‐sensed vegetation data, and bird‐banding data to better understand the dynamics of black‐headed grosbeak (Pheucticus melanocephalus) populations in two breeding regions, the coast and Central Valley of California (Coastal California) and the Sierra Nevada mountain range (Sierra Nevada), over 28 years (1992–2019). Drought conditions across the annual cycle and rainfall timing on the molting grounds influenced seasonal habitat characteristics, including vegetation greenness and phenology (maturity dates). We developed a novel integrated population model with population state informed by adult capture data, recruitment rates informed by age‐specific capture data and climate covariates, and survival rates informed by adult capture–mark–recapture data and climate covariates. Population size was relatively variable among years for Coastal California, where numbers of recruits and survivors were positively correlated, and years of population increase were largely driven by recruitment. In the Sierra Nevada, population size was more consistent and showed stronger evidence of population regulation (numbers of recruits and survivors negatively correlated). Neither region showed evidence of long‐term population trend. We found only weak support for most climate–demographic rate relationships. However, recruitment rates for the Coastal California region were higher when rainfall was relatively early on the molting grounds and when wintering grounds were relatively cool and wet. We suggest that our approach of integrating movement, climate, and demographic data within a novel modeling framework can provide a useful method for better understanding the dynamics of broadly distributed migratory species. 相似文献