全文获取类型
收费全文 | 425篇 |
免费 | 44篇 |
出版年
2021年 | 1篇 |
2020年 | 1篇 |
2019年 | 4篇 |
2018年 | 1篇 |
2017年 | 4篇 |
2016年 | 7篇 |
2015年 | 14篇 |
2014年 | 20篇 |
2013年 | 13篇 |
2012年 | 33篇 |
2011年 | 30篇 |
2010年 | 9篇 |
2009年 | 13篇 |
2008年 | 19篇 |
2007年 | 12篇 |
2006年 | 23篇 |
2005年 | 17篇 |
2004年 | 21篇 |
2003年 | 35篇 |
2002年 | 30篇 |
2001年 | 12篇 |
2000年 | 8篇 |
1999年 | 6篇 |
1998年 | 8篇 |
1997年 | 13篇 |
1996年 | 5篇 |
1995年 | 6篇 |
1994年 | 6篇 |
1993年 | 5篇 |
1992年 | 12篇 |
1991年 | 5篇 |
1990年 | 6篇 |
1989年 | 5篇 |
1988年 | 6篇 |
1987年 | 3篇 |
1986年 | 5篇 |
1985年 | 6篇 |
1984年 | 5篇 |
1983年 | 5篇 |
1982年 | 7篇 |
1981年 | 1篇 |
1980年 | 5篇 |
1979年 | 2篇 |
1978年 | 4篇 |
1977年 | 5篇 |
1976年 | 2篇 |
1975年 | 4篇 |
1974年 | 2篇 |
1972年 | 1篇 |
1958年 | 1篇 |
排序方式: 共有469条查询结果,搜索用时 16 毫秒
421.
J Wessman S Schönauer J Miettunen H Turunen P Parviainen JK Seppänen E Congdon S Service M Koiranen J Ekelund J Laitinen A Taanila T Tammelin M Hintsanen L Pulkki-Råback L Keltikangas-Järvinen J Viikari OT Raitakari M Joukamaa MR Järvelin N Freimer L Peltonen J Veijola H Mannila T Paunio 《PloS one》2012,7(7):e33088
Background
The object of this study was to identify temperament patterns in the Finnish population, and to determine the relationship between these profiles and life habits, socioeconomic status, and health.Methods/Principal Findings
A cluster analysis of the Temperament and Character Inventory subscales was performed on 3,761 individuals from the Northern Finland Birth Cohort 1966 and replicated on 2,097 individuals from the Cardiovascular Risk in Young Finns study. Clusters were formed using the k-means method and their relationship with 115 variables from the areas of life habits, socioeconomic status and health was examined.Results
Four clusters were identified for both genders. Individuals from Cluster I are characterized by high persistence, low extravagance and disorderliness. They have healthy life habits, and lowest scores in most of the measures for psychiatric disorders. Cluster II individuals are characterized by low harm avoidance and high novelty seeking. They report the best physical capacity and highest level of income, but also high rate of divorce, smoking, and alcohol consumption. Individuals from Cluster III are not characterized by any extreme characteristic. Individuals from Cluster IV are characterized by high levels of harm avoidance, low levels of exploratory excitability and attachment, and score the lowest in most measures of health and well-being.Conclusions
This study shows that the temperament subscales do not distribute randomly but have an endogenous structure, and that these patterns have strong associations to health, life events, and well-being. 相似文献422.
CH Chang J Hinkula M Loo T Falkeborn R Li TM Cardillo EA Rossi DM Goldenberg B Wahren 《PloS one》2012,7(7):e41235
We constructed novel HIV-1 fusion inhibitors that may overcome the current limitations of enfuvirtide, the first such therapeutic in this class. The three prototypes generated by the Dock-and-Lock (DNL) technology to comprise four copies of enfuvirtide tethered site-specifically to the Fc end of different humanized monoclonal antibodies potently neutralize primary isolates (both R5-tropic and X4-tropic), as well as T-cell-adapted strains of HIV-1 in vitro. All three prototypes show EC(50) values in the subnanomolar range, which are 10- to 100-fold lower than enfuvirtide and attainable whether or not the constitutive antibody targets HIV-1. The potential of such conjugates to purge latently infected cells was also demonstrated in a cell-to-cell viral inhibition assay by measuring their efficacy to inhibit the spread of HIV-1(LAI) from infected human peripheral blood mononuclear cells to Jurkat T cells over a period of 30 days following viral activation with 100 nM SAHA (suberoylanilide hydroxamic acid). The IgG-like half-life was not significantly different from that of the parental antibody, as shown by the mean serum concentration of one prototype in mice at 72 h. These encouraging results provide a rationale to develop further novel anti-HIV agents by coupling additional antibodies of interest with alternative HIV-inhibitors via recombinantly-produced, self-assembling, modules. 相似文献
423.
424.
X. Zhou Z. M. Ge S. Kellom?ki K. Y. Wang H. Peltola P. J. Martikainen M. Lemettinen A. Hassinen R. Ikonen 《Photosynthetica》2012,50(1):24-34
This paper describes the technical information and performance of a new multi-objective chamber system enabling the control
of environmental variables (e.g., temperature, CO2, air humidity, wind speed, and UV-B radiation) for understanding plant responses to climate change. Over a whole growing
season, four different climate scenarios were evenly programmed into the system’s 16 chambers as ambient environment (AMB),
elevated temperature (ET), elevated CO2 concentration (EC) and elevated temperature and CO2 concentration (ETC). Simultaneously, the chamber effects were assessed regarding the physiological responses and growth of
a boreal perennial grass (reed canary grass, Phalaris arundinacea L.). During the growing season, the chamber system provided a wide variety of climatic conditions for air temperature (T
a), relative humidity (RH) and CO2 concentration (C
a) in the AMB chambers following outside conditions. The target temperature (+3.5°C) was achieved to a good degree in the ET
and ETC chambers, being on average 3.3°C and 3.7°C higher than ambient conditions, respectively. The target concentration
of CO2 (700 ppm) was also well achieved in the EC and ETC chambers, being on average 704 ppm and 703 ppm, respectively. The stable
airflow condition inside all of the chambers provided a homogeneous distribution of gases and temperature. The decreases in
RH and increases in vapour pressure deficit (VPD) in the elevated temperature chambers were also maintained at a low level.
Chamber effects were observed, with some physiological and growth parameters of plants being significantly lower in the AMB
chambers, compared to outside conditions. The plant growth was negatively affected by the reduced radiation inside the chambers. 相似文献
425.
Harri Helaj?rvi Tom Rosenstr?m Katja Pahkala Mika K?h?nen Terho Lehtim?ki Olli J. Heinonen Mervi Oikonen Tuija Tammelin Jorma S. A. Viikari Olli T. Raitakari 《PloS one》2014,9(7)
Background
Television viewing time (TV time) is associated with increased weight and obesity, but it is unclear whether this relation is causal.Methods and Results
We evaluated changes in TV time, waist circumference (waist) and body mass index (BMI) in participants of the population-based Cardiovascular Risk in Young Finns study (761 women, 626 men aged 33–50 years in 2011). Waist and BMI were measured, and TV time was self-reported in 2001, 2007, and 2011. Changes in waist and BMI between 2001 and 2011 were studied a) for the whole group, b) in groups with constantly low (≤1 h/d), moderate (1–3 h/d), or high (≥3 h/d) TV time, and c) in groups with ≥1 hour in-/decrease in daily TV time between 2001 and 2011. BMIs in 1986 were also evaluated. We explored the causal relationship of TV time with waist and BMI by classical temporality criterion and recently introduced causal-discovery algorithms (pairwise causality measures). Both methods supported the hypothesis that TV time is causative to weight gain, and no evidence was found for reverse or bidirectional causality. Constantly low TV time was associated with less pronounced increase in waist and BMI, and waist and BMI increase was lower with decreased TV time (P<0.05). The increase in waist and BMI was at least 2-fold in the high TV time group compared to the low TV time group (P<0.05). Adjustment for age, sex, BMI/waist in 2001, physical activity, energy intake, or smoking did not change the results.Conclusions
In young and middle-aged adults, constantly high TV time is temporally antecedent to BMI and waist increase. 相似文献426.
Microbial Respiration in Arctic Upland and Peat Soils as a Source of Atmospheric Carbon Dioxide 总被引:1,自引:0,他引:1
Christina Biasi Simo Jokinen Maija E. Marushchak Kai Hämäläinen Tatiana Trubnikova Markku Oinonen Pertti J. Martikainen 《Ecosystems》2014,17(1):112-126
Knowledge on soil microbial respiration (SMR) rates and thus soil-related CO2 losses from Arctic soils is vital because of the crucial importance of this ecosystem within the global carbon (C) cycle and climate system. Here, we measured SMR from various habitats during the growing season in Russian subarctic tundra by applying two different approaches: 14C partitioning approach and root trenching. The variable habitats encompassed peat and mineral soils, bare and vegetated surfaces and included both dry and moist ones. The field experiment was complemented by laboratory studies to measure bioavailability of soil carbon and identify sources of CO2. Differences in bioavailability of soils, measured in the laboratory as basal soil respiration rates, were generally greater than inter-site differences in SMR rates measured in situ, suggesting secondary constraints at field conditions, such as soil C content. There was a tendency towards lower SMR in vegetated peat plateaus compared to upland mineral tundra (on average 137 vs. 185 g CO2 m?2 growing season?1, respectively), but no significant differences were found. Surprisingly, the bare surfaces (peat circles) with 3500-year-old C at the surface exhibited about the largest SMR among all sites as shown by both methods. This was related to the general development of peat plateaus in the region, and uplifting of deeper peat with high C content to the surface during the genesis of peat circles. This observation is particularly relevant for decomposition of deeper peat in vegetated peat plateaus, where soil material similar to the bare surfaces can be found. The data indicate that the large stocks of C stored in permafrost peatlands are principally available for decomposition despite old age. 相似文献
427.
Myriam Peyrard-Janvid Elizabeth?J. Leslie Youssef?A. Kousa Tiffany?L. Smith Martine Dunnwald M?ns Magnusson Brian?A. Lentz Per Unneberg Ingegerd Fransson Hannele?K. Koillinen Jorma Rautio Marie Pegelow Agneta Karsten Lina Basel-Vanagaite William Gordon Bogi Andersen Thomas Svensson Jeffrey?C. Murray Robert?A. Cornell Juha Kere Brian?C. Schutte 《American journal of human genetics》2014,94(1):23-32
Mutations in interferon regulatory factor 6 (IRF6) account for ∼70% of cases of Van der Woude syndrome (VWS), the most common syndromic form of cleft lip and palate. In 8 of 45 VWS-affected families lacking a mutation in IRF6, we found coding mutations in grainyhead-like 3 (GRHL3). According to a zebrafish-based assay, the disease-associated GRHL3 mutations abrogated periderm development and were consistent with a dominant-negative effect, in contrast to haploinsufficiency seen in most VWS cases caused by IRF6 mutations. In mouse, all embryos lacking Grhl3 exhibited abnormal oral periderm and 17% developed a cleft palate. Analysis of the oral phenotype of double heterozygote (Irf6+/−;Grhl3+/−) murine embryos failed to detect epistasis between the two genes, suggesting that they function in separate but convergent pathways during palatogenesis. Taken together, our data demonstrated that mutations in two genes, IRF6 and GRHL3, can lead to nearly identical phenotypes of orofacial cleft. They supported the hypotheses that both genes are essential for the presence of a functional oral periderm and that failure of this process contributes to VWS. 相似文献
428.
429.
The effects of green tree retention and subsequent prescribed burning on ground beetles (Coleoptera: Carabidae) in boreal pine-dominated forests 总被引:3,自引:0,他引:3
We studied how two methods to promote biodiversity in managed forests, i.e. green tree retention and prescribed fire, affect the assemblages of carabid beetles. Our experiment consisted of 24 study sites, each 3–5 ha in size, which had been prepared according to factorial design. Each of the eight treatment combinations determined by the two factors explored – tree retention level (0, 10, 50 m3/ha?1 and uncut controls) and prescribed use of fire (yes/no) – was replicated three times. We sampled carabids using pitfall traps one year after the treatments. Significantly more individuals were caught in most of the burned sites, but this difference was partially reflective of the trap‐catches of Pterostichus adstrictus. The fire did not increase no. of P. adstrictus in the uncut sites as much as in the other sites. Species richness was significantly affected by both factors, being higher in the burned than in the unburned sites and in the harvested than in the unharvested sites. Many species were concentrated in the groups of retention trees in the burned sites, but only a few were in the unburned sites. The species turnover was greater in the burned than in the unburned sites, as indicated by the NMDS ordinations. Greater numbers of smaller sized species and proportion of brachypterous species were present in the burned sites. Fire‐favored species, and also the majority of other species that prefer open habitats were more abundantly caught in the burned sites than in the unburned sites. Dead wood or logging waste around the traps did not correlate with the occurrence of species. We conclude that carabids are well adapted to disturbances, and that frequent use of prescribed fire is essential for the maintenance of natural assemblages of carabid beetles in the boreal forest. Small retention tree groups can not maintain assemblages of uncut forest, but they can be important by providing food, shelter and breeding sites for many species, particularly in the burned sites. 相似文献
430.
P. Martikainen J. Kouki O. Heikkala E. Hyvärinen H. Lappalainen 《Journal of Applied Entomology》2006,130(1):37-44
Abstract: We explored how two recently introduced methods to promote biodiversity during the timber harvest in boreal forests – green tree retention and use of fire – may affect damages caused by pine shoot beetles (Tomicus piniperda and Tomicus minor; Col., Scolytinae) in the surrounding pine forests. The experimental design included 24 separate study sites, 3–5 ha each, which were assigned to different treatments according to factorial design. Retention levels included 0, 10, 50 m3/ha and uncut controls. Twelve of the sites were burned and thus there were three replicates of each treatment combination. Old and new fallen pine shoots were counted from transects in adjacent pine‐dominated forests 2 and 3 years after the treatments. Populations of Tomicus spp. in the sites were monitored using window traps before and after the treatments, and by examining felled sample trees. Numbers of fallen shoots returned to background levels around unburned and burned clearcuts with no retention trees in 3 years after the treatments, but remained still at increased level in burned harvested sites with 10 and 50 m3 of retained trees per hectare. Numbers of fallen shoots in burned uncut forests increased in one site only, where the fire was intense enough to kill large pine trees, but the damage did not extend outside the burned area. Shoot numbers remained at such low levels (<18 000 shoots/ha) in all treatment combinations that growth losses were not likely. Numbers of egg galleries of Tomicus spp. in trees killed by fire were low, indicating that burnings that take place after the swarming of Tomicus beetles create dead wood that is not optimal for the breeding of these pests. We conclude that green tree retention and prescribed use of fire do not automatically affect populations of Tomicus spp. more than traditional forestry operations (thinnings and clearcuttings) do. 相似文献