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11.
Claudia Raedig Carsten F. Dormann Anke Hildebrandt Sven Lautenbach 《Biodiversity and Conservation》2010,19(6):1523-1546
Monographic data rely on specimens deposited in herbaria and museums, which have been thoroughly revised by experts. However,
monographic data have been rarely used to map species richness at large scale, mainly because of the difficulties caused by
spatially heterogeneous sampling effort. In this paper we estimate patterns of species richness and narrow endemism, based
on monographic data of 4,055 Neotropical angiosperm species. We propose a geometric interpolation method to derive species
ranges at a 1° grid resolution. To this we apply an inverse distance-weighted summation scheme to derive maps of species richness
and endemism. In the latter we also adjust for heterogeneous sampling effort. Finally, we test the robustness of the interpolated
species ranges and derived species richness by applying the same method but using a leave-one-out-cross-validation (LOOCV).
The derived map shows four distinct regions of elevated species richness: (1) Central America, (2) the Northern Andes, (3)
Amazonia and (4) the Brazilian Atlantic coast (‘Mata Atlantica’). The region with the highest estimated species richness is
Amazonia, with Central America following closely behind. Centers of narrow endemism are located over the entire Neotropics,
several of them coinciding with regions of elevated species richness. Sampling effort has a minor influence on the interpolation
of overall species richness, but it substantially influences the estimation of regions of narrow endemism. Thus, in order
to improve maps of narrow endemism and resulting conservation efforts, more collection and identification activity is required. 相似文献
12.
P C de Visser N M A J Kriek P A V van Hooft A Van Schepdael D V Filippov G A van der Marel H S Overkleeft J H van Boom D Noort 《The journal of peptide research》2003,61(6):298-306
As part of a program towards the development of novel antibiotics, a convenient method for solid-phase synthesis of the cyclic cationic peptide polymyxin B1 and analogues thereof is described. The methodology, based on cleavage-by-cyclization using Kenner's safety-catch linker, yields crude products with purities ranging from 37-67%. Antibacterial assays revealed that analogues 23-26, in which the (S)-6-methyloctanoic acid moiety is replaced with shorter acyl chains, exhibit distinct antimicrobial activity. The results suggest that the length of the acyl chain is rather critical for antimicrobial activity. On the other hand, substitution of the hydrophobic ring-segment D-Phe-6/Leu-7 in polymyxin B1 with dipeptide mimics (i.e. analogues 27-33) resulted in almost complete loss of antimicrobial activity. 相似文献
13.
R N Korotkina A A Papin A A Karelin 《Biulleten' eksperimental'no? biologii i meditsiny》1985,100(10):438-440
Experiments on 330 rats were made to study the influence of benzodiazepines (diazepam, dormicum and phenazepam) on 5'-nucleotidase activity in brain homogenates. It was discovered that diazepam and dormicum in doses of 3 and 4 mg, phenazepam in doses of 3.75 and 5 mg per 200 g bw provoked a 16-20% reduction in 5'-nucleotidase activity. The maximal effect of diazepam (3 and 4 mg doses) was attained 1 h after intraperitoneal injection, that of dormicum (3 mg) 30 min and of phenazepam (5 mg) 1 h after intraperitoneal injection. It is assumed that benzodiazepines are involved in AMP metabolism. 相似文献
14.
R. A. Mueller M. J. Rosner J. N. Ghia S. K. Powers E. R. Kafer R. D. Hunt 《Cellular and molecular neurobiology》1988,8(2):235-243
1. Examination of the cerebrospinal fluid (CSF) of head-injured patients reveals that the concentration of intraventricular xanthine is elevated and that of uridine is decreased relative to those of adult lumbar CSF. 2. No correlations were observed between CSF lactate and CSF hypoxanthine, xanthine, or uridine, suggesting that changes in purine metabolites and the pyrimidine nucleoside do not index similar cellular events as does lactic acid production. 3. Ventricular CSF from hydrocephalic infants had uridine and hypoxanthine concentrations not significantly different from those of normal adult lumbar CSF, but xanthine was significantly elevated. 4. Since uridine has anticonvulsant properties and is a crucial substrate for cerebral metabolism, it may be useful to evaluate this pyrimidine for use in the management of patients with head injury. 相似文献
15.
16.
Fluorescence technique for comparative studies of substrate-binding subsites in serine proteinases. Application to subtilisins. 下载免费PDF全文
A fluorescence technique for comparative studies of substrate-binding subsites in serine proteinases is described. It consists of: selective labelling of the corresponding subsites with a fluorescent group by using N alpha-dansyl(5-dimethylaminonaphthalene-1-sulphonyl)ated peptide chloromethanes containing different numbers of amino acid residues, and probing the immediate environment of the subsites by quenching experiments using ionic and neutral quenchers. Intramolecular distances between the subsites and particular chromophores can be also determined. The technique is of general applicability to all serine proteinases. The above mentioned approach was applied to two proteinases: subtilisin Novo and mesentericopeptidase. It was concluded that the substrate-binding site of mesentericopeptidase is considerably more polar than that of subtilisin Novo. Intramolecular distances between the labelled subsites and tryptophan residues in the two proteinases were determined. 相似文献
17.
Inositol 1,4,5-trisphosphate is not effective in releasing calcium from skeletal sarcoplasmic reticulum microsomes 总被引:2,自引:0,他引:2
Regulation of many cell systems has been shown to be mediated by Inositol 1,4,5-trisphosphate which causes a release of calcium from intracellular sites. We have shown that release of Ca2+ from sarcoplasmic reticulum microsomes was not stimulated by IP3. The phorbol ester, TPA, also had no effect on Ca2+ release or Ca2+ ATPase activity. Thus, it is unlikely that the breakdown of polyphosphatidylinositides serves as a second messenger to mediate release of Ca2+ in skeletal muscle. 相似文献
18.
V A Berezin E N Zhmarev I A Brodskaia G M Shevchenko L S Zashko 《Biulleten' eksperimental'no? biologii i meditsiny》1985,100(7):68-70
The content of neurospecific proteins S-100, GFA and D2 was measured in malignant cerebral tumors by electrophoresis with the use of monospecific antisera. Concomitant measurement of proteins S-100 and GFA is a more reliable diagnostic criterion as to the tumor histogenesis than study of each protein alone. D2 protein appeared to be the most stable specific marker. 相似文献
19.
Sacramento Doralice Rodrigues Coelho Rosalie Reed Rodrigues Wigg Márcia Dutra Toledo Luna Linhares Luiz Fernando de Matos dos Santos Marta Gonçalves Azevedo Soares Semêdo Luzia Teixeira de Ribeiro da Silva Antonio Jorge 《World journal of microbiology & biotechnology》2004,20(3):225-229
A promising producer of bioactive compounds isolated from a Brazilian tropical soil was tested for its range of antimicrobial
activities. Strain 606, classified as Streptomyces sp., could not be identified up to species level, suggesting a possible new taxon. The supernatant and 10 extracts and fractions,
obtained by extraction and chromatographic techniques, presented antimicrobial activity using antibiograms. The methanolic
fraction was highly active against pathogenic bacteria, phytopathogenic fungi and the human pathogenic yeast Candida albicans. It also possessed high antiviral activity inhibiting the propagation of an acyclovir-resistant herpes simplex virus type
1 strain on HEp-2 cells at non-cytotoxic concentration. The strong cytotoxic effect suggests an antitumour action.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
20.
Hostile intercommunity relations, including attacking and killing extra-community infants of both sexes have occurred at most
wild chimpanzee sites. We describe three recent cases of intercommunity attacks on infants committed by members of the Ngogo
chimpanzee community in Kibale National Park, Uganda. Two of the attacks resulted in confirmed infanticides while a third
attack probably resulted in the infant's death. In common with previous accounts of chimpanzee infanticides, the attacks described
here occurred during boundary patrols outside the Ngogo community's usual range, adult and adolescent males were the main
participants, one infant was cannibalized after being killed, and the victims’ mothers did not accompany the attacking party
back to the Ngogo range. However, the patrol parties during each infanticide were larger than before and included females
from the Ngogo community. Our observations indirectly support both the range expansion and imbalance of power hypotheses,
which address why and under which conditions chimpanzee intercommunity encounters lead to aggression. These cases of intercommunity
infanticide add to the growing database of the phenomenon in wild chimpanzees. 相似文献