全文获取类型
收费全文 | 109713篇 |
免费 | 5477篇 |
国内免费 | 75篇 |
出版年
2018年 | 1066篇 |
2017年 | 1241篇 |
2016年 | 3015篇 |
2015年 | 6384篇 |
2014年 | 6063篇 |
2013年 | 6056篇 |
2012年 | 5217篇 |
2011年 | 2537篇 |
2010年 | 2464篇 |
2009年 | 2363篇 |
2008年 | 1047篇 |
2007年 | 1058篇 |
2006年 | 1066篇 |
2005年 | 7159篇 |
2004年 | 5852篇 |
2003年 | 4102篇 |
2002年 | 1689篇 |
2001年 | 2849篇 |
2000年 | 2109篇 |
1999年 | 2781篇 |
1998年 | 777篇 |
1997年 | 626篇 |
1996年 | 575篇 |
1992年 | 2869篇 |
1991年 | 2967篇 |
1990年 | 3024篇 |
1989年 | 2936篇 |
1988年 | 2818篇 |
1987年 | 2629篇 |
1986年 | 2308篇 |
1985年 | 2329篇 |
1984年 | 1625篇 |
1983年 | 1296篇 |
1982年 | 807篇 |
1981年 | 745篇 |
1980年 | 679篇 |
1979年 | 1591篇 |
1978年 | 1169篇 |
1977年 | 990篇 |
1976年 | 942篇 |
1975年 | 1297篇 |
1974年 | 1424篇 |
1973年 | 1391篇 |
1972年 | 1323篇 |
1971年 | 1217篇 |
1970年 | 1058篇 |
1969年 | 1058篇 |
1968年 | 945篇 |
1967年 | 944篇 |
1966年 | 746篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
21.
22.
23.
24.
25.
A new simple enzymatic method for measuring AMP content in freeze-clamped rat heart is presented. The method is based on the ammonia estimation after the deamination of 5'-AMP by muscle 5'-adenylic acid deaminase. The minimum detectable amount of AMP was about 1.5 nmol. The recovery of AMP added to the tissue homogenate was 94%. The variance coefficient evaluated by assaying five samples from one tissue extract was equal to 5%. AMP content of rat heart (0.28 mumol/g wet tissue) is comparable with the values reported by others. 相似文献
26.
S-Protein/vitronectin is a serum glycoprotein that inhibits the lytic activity of the membrane attack complex of complement, i.e., of the complex including the proteins C5b, C6, C7, C8, and C9n. We show that intact S-protein/vitronectin or its cyanogen bromide generated fragments also inhibit the hemolysis mediated by perforin from cytotoxic T-cells at 45 and 11 microM, respectively. The glycosaminoglycan binding site of S-protein/vitronectin is responsible for the inhibition, since a synthetic peptide corresponding to a part of this highly basic domain (amino acid residues 348-360) inhibits complement- as well as perforin-mediated cytolysis. In the case of C9, the synthetic peptide binds to the acidic residues occurring in its N-terminal cysteine-rich domain (residues 101-111). Antibodies raised against this particular segment react 25-fold better with the polymerized form of C9 as compared with its monomeric form, indicating that this site becomes exposed only upon the hydrophilic-amphiphilic transition of C9. Since the cysteine-rich domain of C9 has been shown to be highly conserved in C6, C7, and C8 as well as in perforin, the inhibition of the lytic activities of these molecules by S-protein/vitronectin or by peptides corresponding to its heparin binding site may be explained by a similar mechanism. 相似文献
27.
28.
Abstract Electrofusion between cells of yeast strains with different genetic markers in isotonic sorbitol solutions leads to high yields of hybrids when 0.1 mM Ca2+ and 0.5 mM Mg2+ salts are aded. On average, 1000–2000 hybrids are obtained when electrofusion is performed (in a helical chamber) compared to a yield of about 40–120 in the absence of these bivalent cations. A further increase in yield can be achieved by the addition of 1 mg/ml albumin, which results in up to 4000 hybrids per experimental run. The entire fusion process leads to very reproducible results in the presence of these substances. 相似文献
29.
30.