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101.
Permafrost thaw resulting from climate warming may dramatically change the succession and carbon dynamics of northern ecosystems.
To examine the joint effects of regional temperature and local species changes on peat accumulation following thaw, we studied
peat accumulation across a regional gradient of mean annual temperature (MAT). We measured aboveground net primary production
(AGNPP) and decomposition over 2 years for major functional groups and used these data to calculate a simple index of net
annual aboveground peat accumulation. In addition, we collected cores from six adjacent frozen and thawed bog sites to document
peat accumulation changes following thaw over the past 200 years. Aboveground biomass and decomposition were more strongly
controlled by local succession than regional climate. AGNPP for some species differed between collapse scars and associated
permafrost plateaus and was influenced by regional MAT. A few species, such as Picea mariana trees on frozen bogs and Sphagnum mosses in thawed bogs, sequestered a disproportionate amount of peat; in addition, changes in their abundance following thaw
changed peat accumulation. 210Pb-dated cores indicated that peat accumulation doubles following thaw and that the accumulation rate is affected by historical
changes in species during succession. Peat accumulation in boreal peatlands following thaw was controlled by a complex mix
of local vegetation changes, regional climate, and history. These results suggest that northern ecosystems may show responses
more complex than large releases of carbon during transient warming.
Received 8 August 2000; accepted 12 January 2001. 相似文献
102.
Shahar Rotem-Bamberger Jamal Fahoum Keren Keinan-Adamsky Tomer Tsaban Orly Avraham Deborah E. Shalev Jordan H. Chill Ora Schueler-Furman 《The Journal of biological chemistry》2022,298(8)
Class I WW domains are present in many proteins of various functions and mediate protein interactions by binding to short linear PPxY motifs. Tandem WW domains often bind peptides with multiple PPxY motifs, but the interplay of WW–peptide interactions is not always intuitive. The WW domain–containing oxidoreductase (WWOX) harbors two WW domains: an unstable WW1 capable of PPxY binding and stable WW2 that cannot bind PPxY. The WW2 domain has been suggested to act as a WW1 domain chaperone, but the underlying mechanism of its chaperone activity remains to be revealed. Here, we combined NMR, isothermal calorimetry, and structural modeling to elucidate the roles of both WW domains in WWOX binding to its PPxY-containing substrate ErbB4. Using NMR, we identified an interaction surface between these two domains that supports a WWOX conformation compatible with peptide substrate binding. Isothermal calorimetry and NMR measurements also indicated that while binding affinity to a single PPxY motif is marginally increased in the presence of WW2, affinity to a dual-motif peptide increases 10-fold. Furthermore, we found WW2 can directly bind double-motif peptides using its canonical binding site. Finally, differential binding of peptides in mutagenesis experiments was consistent with a parallel N- to C-terminal PPxY tandem motif orientation in binding to the WW1–WW2 tandem domain, validating structural models of the interaction. Taken together, our results reveal the complex nature of tandem WW-domain organization and substrate binding, highlighting the contribution of WWOX WW2 to both protein stability and target binding. 相似文献
103.
Rainer Benndorf Ryan Velazquez Jordan D. Zehr Sergei L. Kosakovsky Pond Jody L. Martin Alexander G. Lucaci 《Cell stress & chaperones》2022,27(4):309
Small heat shock proteins (sHSPs) emerged early in evolution and occur in all domains of life and nearly in all species, including humans. Mutations in four sHSPs (HspB1, HspB3, HspB5, HspB8) are associated with neuromuscular disorders. The aim of this study is to investigate the evolutionary forces shaping these sHSPs during vertebrate evolution. We performed comparative evolutionary analyses on a set of orthologous sHSP sequences, based on the ratio of non-synonymous: synonymous substitution rates for each codon. We found that these sHSPs had been historically exposed to different degrees of purifying selection, decreasing in this order: HspB8 > HspB1, HspB5 > HspB3. Within each sHSP, regions with different degrees of purifying selection can be discerned, resulting in characteristic selective pressure profiles. The conserved α-crystallin domains were exposed to the most stringent purifying selection compared to the flanking regions, supporting a ''dimorphic pattern'' of evolution. Thus, during vertebrate evolution the different sequence partitions were exposed to different and measurable degrees of selective pressures. Among the disease-associated mutations, most are missense mutations primarily in HspB1 and to a lesser extent in the other sHSPs. Our data provide an explanation for this disparate incidence. Contrary to the expectation, most missense mutations cause dominant disease phenotypes. Theoretical considerations support a connection between the historic exposure of these sHSP genes to a high degree of purifying selection and the unusual prevalence of genetic dominance of the associated disease phenotypes. Our study puts the genetics of inheritable sHSP-borne diseases into the context of vertebrate evolution. Supplementary InformationThe online version contains supplementary material available at 10.1007/s12192-022-01268-y. 相似文献
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108.
Chr Jordan R. V. Sengbusch 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1957,27(4):186-187
Ohne ZusammenfassungMit 2 TextabbildungenDiese Arbeit wurde mit Unterstützung der Deutschen Forschungsgemeinschaft durchgeführt. 相似文献
109.
Chr Jordan F. Korte R. von Sengbusch 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1957,27(2):69-76
Ohne ZusammenfassungMit 24 TextabbildungenDiese Arbeiten wurden mit Unterstützung der Deutschen Forschungsgemeinschaft durchgeführt. 相似文献
110.