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排序方式: 共有154条查询结果,搜索用时 15 毫秒
1.
Lens crystallins and their gene families 总被引:11,自引:0,他引:11
J Piatigorsky 《Cell》1984,38(3):620-621
2.
Joram Piatigorsky Barbara Norman Raymond E. Jones 《Journal of molecular evolution》1987,25(4):308-317
A cloned chicken delta-crystallin cDNA was used to identify two putative delta-crystallin genes in the duck by Southern blot hybridization. A DNA fragment containing most of one of these genes was isolated from a library made in bacteriophage lambda Charon 28A containing genomic DNA from 14-day-old embryonic ducks. Electron microscopy, partial gene sequencing, primer extension analysis using duck mRNA, and comparison with the well-characterized chicken delta-crystallin genes suggest that our cloned duck delta-crystallin gene, like the chicken delta-crystallin genes, is 8-10 kb long and contains 17 exons. Hybridization and sequencing data show great similarity between the homologous 5' untranslated and coding exons of the duck and chicken delta-crystallin genes. Overall, the homologous introns also appear to have approximately 30% sequence similarity, and have been subject to deletion/insertion events. Our partial characterization of duck delta-crystallin gene sequences suggests that this avian and reptilian crystallin family has been conserved during evolution, as have the other crystallin gene families that are expressed in the eye lens. 相似文献
3.
4.
Binding of nuclear proteins to promoter elements of the mouse alpha A-crystallin gene 总被引:10,自引:0,他引:10
B Sommer A B Chepelinsky J Piatigorsky 《The Journal of biological chemistry》1988,263(30):15666-15672
5.
Trehalose dimycolate, a glycolipid component of the cell walls of mycobacteria, induces interstitial pneumonitis and alveolar hemorrhages in C57BL/6 and C57BL/10 mice. Homozygous nude (nu/nu) mice of these backgrounds are not susceptible to this form of pulmonary injury. However, after administration of T-lymphocyte-enriched spleen cell preparations from syngeneic donors, homozygous nude mice become susceptible to trehalose dimycolate. The observations suggest that production of pulmonary lesions by this mycobacterial component is dependent on T lymphocytes. While the mechanisms are still under study, we propose that trehalose dimycolate can function as an activator of T lymphocytes and that products of activated T cells are responsible for production of the pulmonary lesions. 相似文献
6.
Preferential conservation of the globular domains of the beta A3/A1-crystallin polypeptide of the chicken eye lens 总被引:4,自引:0,他引:4
The primary structure of the beta 19/26-crystallin polypeptide of the chicken lens has been determined by cDNA sequencing and primer extension experiments. In addition, a primer extension experiment has corrected the sequence for the N-terminal arm of the murine beta 23 polypeptide, which is the homologue of the chicken beta 19/26 polypeptide. We also show that, in the chicken and mouse, the N-terminal arm of the polypeptide is encoded on two separate exons. For simplicity, we have changed the names of both chicken beta 19/26 and murine beta 23 to beta A3/A1, which is the name of the homologous bovine polypeptide. The deduced sequence of the chicken beta A3/A1 polypeptide fits the predicted three-dimensional structure involving two homologous domains, each folded into two 'Greek key' motifs, common to the beta gamma-crystallin superfamily of proteins. Comparison of the amino acid sequence of the chicken and mammalian beta A3/A1 polypeptides indicates that different regions of the protein, which are encoded on different exons, are diverging at different rates. The N-terminal extension is the fastest evolving region of the beta A3/A1 polypeptide. Hybrid-selected translation coupled with primer extension experiments suggest that a single chicken beta A3/A1 mRNA synthesizes two polypeptides, beta A3 (25 kDa) and beta A1 (23 kDa) by utilization of different translation initiation sites. 相似文献
7.
8.
Julielani T. Ngo Ivana Klisak Robert A. Dubin Joram Piatigorsky T. Mohandas Robert S. Sparkes J. Bronwyn Bateman 《Genomics》1989,5(4)
Using a human αB-crystallin genomic probe and human-mouse somatic cell hybrids, the human αB-gene was assigned to chromosome 11 and further corroborated by in situ hybridization to normal metaphase chromosomes. This assignment confirmed and regionally mapped the locus to q22.3–23.1. 相似文献
9.
Tissue-specific expression of the chicken alpha A-crystallin gene in cultured lens epithelia and transgenic mice 总被引:10,自引:0,他引:10
J F Klement E F Wawrousek J Piatigorsky 《The Journal of biological chemistry》1989,264(33):19837-19844
The present experiments show that the single gene for the lens-specific protein alpha A-crystallin of chickens and mice uses a different subset of cis- and trans-acting regulatory elements for expression in transfected embryonic chicken lens epithelial cells. A chicken alpha A-crystallin-chloramphenicol acetyltransferase (CAT) fusion gene required 162 base pairs whereas the murine alpha A-crystallin-CAT fusion gene required only 111 base pairs of 5'-flanking sequences for efficient tissue-specific expression in the transfected chicken lens cells. Gel retardation and competition experiments were performed using embryonic chicken lens nuclear extract and oligodeoxynucleotides identical to the 5'-flanking region of the chicken (-170/-111) and murine (-111/-88 and -88/-55) alpha A-crystallin gene. The results indicated that these homologous promoters use different nuclear factors for function. Methylation interference analysis identified a dyad of symmetry (CTGGTTCCCACCAG) at position -153 to -140 in the chicken alpha A-crystallin promoter which binds one or more lens nuclear factors. Gel mobility shift experiments using nuclear extracts of brain, reticulocytes, and muscle of embryonic chickens or HeLa cells suggested that the factor(s) binding to the chicken alpha A-crystallin gene promoter sequences are not lens specific. Despite differences in the functional and protein-binding properties of the alpha A-crystallin gene promoter of chickens and mice, expression of the chicken alpha A-crystallin-CAT fusion gene in transgenic mice was lens specific, consistent with a common underlying mechanism for expression of the alpha A-crystallin gene in chickens and mice. 相似文献
10.
A complex array of positive and negative elements regulates the chicken alpha A-crystallin gene: involvement of Pax-6, USF, CREB and/or CREM, and AP-1 proteins. 总被引:9,自引:3,他引:6 下载免费PDF全文
The abundance of crystallins (> 80% of the soluble protein) in the ocular lens provides advantageous markers for selective gene expression during cellular differentiation. Here we show by functional and protein-DNA binding experiments that the chicken alpha A-crystallin gene is regulated by at least five control elements located at sites A (-148 to -139), B (-138 to -132), C (-128 to -101), D (-102 to -93), and E (-56 to -41). Factors interacting with these sites were characterized immunologically and by gel mobility shift experiments. The results are interpreted with the following model. Site A binds USF and is part of a composite element with site B. Site B binds CREB and/or CREM to enhance expression in the lens and binds an AP-1 complex including CREB, Fra2 and/or JunD which interacts with USF on site A to repress expression in fibroblasts. Sites C and E (which is conserved across species) bind Pax-6 in the lens to stimulate alpha A-crystallin promoter activity. These experiments provide the first direct data that Pax-6 contributes to the lens-specific expression of a crystallin gene. Site D (-104 to -93) binds USF and is a negative element. Thus, the data indicate that USF, CREB and/or CREM (or AP-1 factors), and Pax-6 bind a complex array of positive and negative cis-acting elements of the chicken alpha A-crystallin gene to control high expression in the lens and repression in fibroblasts. 相似文献