全文获取类型
收费全文 | 2051篇 |
免费 | 122篇 |
国内免费 | 94篇 |
出版年
2023年 | 21篇 |
2022年 | 44篇 |
2021年 | 91篇 |
2020年 | 51篇 |
2019年 | 56篇 |
2018年 | 56篇 |
2017年 | 51篇 |
2016年 | 70篇 |
2015年 | 141篇 |
2014年 | 105篇 |
2013年 | 144篇 |
2012年 | 190篇 |
2011年 | 172篇 |
2010年 | 100篇 |
2009年 | 68篇 |
2008年 | 122篇 |
2007年 | 96篇 |
2006年 | 82篇 |
2005年 | 76篇 |
2004年 | 57篇 |
2003年 | 65篇 |
2002年 | 43篇 |
2001年 | 57篇 |
2000年 | 38篇 |
1999年 | 51篇 |
1998年 | 16篇 |
1997年 | 13篇 |
1996年 | 13篇 |
1995年 | 12篇 |
1994年 | 10篇 |
1993年 | 7篇 |
1992年 | 20篇 |
1991年 | 15篇 |
1990年 | 20篇 |
1989年 | 22篇 |
1988年 | 6篇 |
1987年 | 5篇 |
1986年 | 6篇 |
1985年 | 6篇 |
1983年 | 3篇 |
1979年 | 3篇 |
1978年 | 5篇 |
1975年 | 3篇 |
1974年 | 3篇 |
1972年 | 3篇 |
1971年 | 3篇 |
1970年 | 5篇 |
1967年 | 2篇 |
1966年 | 2篇 |
1956年 | 2篇 |
排序方式: 共有2267条查询结果,搜索用时 15 毫秒
961.
962.
963.
Arsenic Carcinogenesis in the Skin 总被引:9,自引:0,他引:9
Summary Chronic arsenic poisoning is a world public health issue. Long-term exposure to inorganic arsenic (As) from drinking water has been documented to induce cancers in lung, urinary bladder, kidney, liver and skin in a dose–response relationship. Oxidative stress, chromosomal abnormality and altered growth factors are possible modes of action in arsenic carcinogenesis. Arsenic tends to accumulate in the skin. Skin hyperpigmentation and hyperkeratosis have long been known to be the hallmark signs of chronic As exposure. There are significant associations between these dermatological lesions and risk of skin cancer. The most common arsenic-induced skin cancers are Bowen’s disease (carcinoma in situ), basal cell carcinoma (BCC) and squamous cell carcinoma (SCC). Arsenic-induced Bowen’s disease (As-BD) is able to transform into invasive BCC and SCC. Individuals with As-BD are considered for more aggressive cancer screening in the lung and urinary bladder. As-BD provides an excellent model for studying the early stages of chemical carcinogenesis in human beings. Arsenic exposure is associated with G2/M cell cycle arrest and DNA aneuploidy in both cultured keratinocytes and As-BD lesions. These cellular abnormalities relate to the p53 dysfunction induced by arsenic. The characteristic clinical figures of arsenic-induced skin cancer are: (i) occurrence on sun-protected areas of the body; (ii) multiple and recrudescent lesions. Both As and UVB are able to induce skin cancer. Arsenic treatment enhances the cytotoxicity, mutagenicity and clastogenicity of UV in mammalian cells. Both As and UVB induce apoptosis in keratinocytes by caspase-9 and caspase-8 signaling, respectively. Combined UVB and As treatments resulted in the antiproliferative and proapoptotic effects by stimulating both caspase pathways in the keratinocytes. UVB irradiation inhibited mutant p53 and ki-67 expression, as well as increased in the number of apoptotic cells in As-BD lesions which resulted in an inhibitory effect on proliferation. As-UVB interaction provides a reasonable explanation for the rare occurrences of arsenical cancer in the sun-exposed skin. The multiple and recurrent skin lesions are associated with cellular immune dysfunction in chronic arsenism. A decrease in peripheral CD4+ cells was noticed in the inhabitants of arsenic exposure areas. There was a decrease in the number of Langerhans cells in As-BD lesion which results in an impaired immune function on the lesional sites. Since CD4+ cells are the target cell affected by As, the interaction between CD4+ cells and epidermal keratinocytes under As affection might be closely linked to the pathogenesis of multiple occurrence of arsenic-induced skin cancer. In this review, we provide and discuss the pathomechanisms of arsenic skin cancer and the relationship to its characteristic figures. Such information is critical for understanding the molecular mechanism for arsenic carcinogenesis in other internal organs. 相似文献
964.
Yuxiu Zhang Jinmei Li Fei Yu Lin Cong Liyan Wang Gérard Burkard Tuanyao Chai 《Molecular biotechnology》2006,32(3):205-217
A heavy metal responsive gene PvSR3 (GenBank accession number U54703) encoding an acid dehydrin was isolated from a mercuric chloride-treated bean (Phaseolus vulgaris L.) leaf cDNA library by differential screening using cDNAs derived from treated and untreated plants. The PvSR3 cDNA is 981-bp long and has a 606-bp open-reading frame with a 202-residue-deduced amino acid sequence. The PvSR3 sequence
contains two conserved repeats of the characteristic lysine-rich K segment (EKKGIMDKIKEKLPG) preceded by an 8-serine residue
stretch, whereas the Y segment (DEYGNP) conserved motif is absent. The deduced protein has a calculated molecular weight of
23 kDa and an isoelectric point of 5.2. Sequence similarity and comparative analysis showed that PvSR3 shares 70 and 73% similarity
with the dehydrin of poplar and pepper, respectively. Southern hybridizations indicated that PvSR3 was a low copy-number gene. Northern blot analysis revealed that PvSR3 mRNA was weakly detected in seedling leaves. However, the gene expression was strongly stimulated by heavy metals, such as
mercury, cadmium, arsenic, and coppper, whereas virus infection and salt had little effect on it. In contrast, PvSR3 was not responsive to drought or abscisic acid (ABA), and was downregulated by UV radiation. Furthermore, PvSR3 was upregulated by the exogenous signaling molecules, including salicylic acid (SA) and hydrogen peroxide (H2O2). It is suggested that PvSR3 is extremely related to heavy metal stress, and might play an important role in metal detoxification and resistance to the
damage caused by heavy metals. 相似文献
965.
In type 1 diabetes mellitus (T1DM), the processes which control the recruitment of immune cells into pancreatic islets are poorly defined. Complex interactions involving adhesion molecules, chemokines and chemokine receptors may facilitate this process. The chemokine, monocyte chemoattractant protein-1 (MCP-1), previously shown to be important in leukocyte trafficking in other disease systems, may be a key participant in the early influx of blood-borne immune cells into islets during T1DM. In the non-obese diabetic (NOD) mouse, the expression of MCP-1 protein has not been demonstrated. We employed dual-label immunohistochemistry to examine the intra-islet expression, distribution and cellular source of MCP-1 in the NOD mouse following cyclophosphamide administration. NOD mice were treated with cyclophosphamide at day 72–73 and MCP-1 expression studied at days 0, 4, 7, 11 and 14 after treatment and comparisons were made between age-matched NOD mice treated with diluent and non-diabetes-prone CD-1 mice. Pancreatic expression of MCP-1 was also examined in NOD mice at various stages of spontaneous diabetes. In the cyclophosphamide group at day 0, MCP-1 immunolabelling was present in selective peri-islet macrophages but declined at day 4. It increased slightly at day 7 but was more marked from day 11, irrespective of diabetes development. The pattern of MCP-1 expression in macrophages was different over time in both the cyclophosphamide and control groups. In the cyclophosphamide group, there was a change over time with an increase at day 11. In the control group, there was little evidence of change over time. There was no significant difference in the mean percentage of MCP-1 positive macrophages between the cyclophosphamide-treated diabetic and non-diabetic mice. During spontaneous diabetes in the NOD mouse, only a few peri-islet MCP-1 cells appeared at day 45. These became more numerous from day 65 but were absent at diabetes onset. We speculate that a proportion of early islet-infiltrating macrophages which express MCP-1 may attract additional lymphocytes and macrophages into the early inflamed islets and intensify the process of insulitis. 相似文献
966.
Shin EH Kim IM Kim JL Han ET Park YK Nawa Y Kook J Lee SH Chai JY 《The Journal of parasitology》2006,92(2):223-229
Of morphologically indistinguishable small-sized neodiplostomula in the grass snake Rhabdophis tigrina in the Republic of Korea, some, such as Neodiplostomum seoulense, mature into adults in the intestines of rodents, whereas others, such as Neodiplostomum leei, migrate to rodent livers and mature in the intestines of chicks. In the present study, we aimed to observe in more detail the extraintestinal migration and development of N. leei by using various animal models, i.e., mice, rats, hamsters, rabbits, cats, and chicks. In mammals, small-sized neodiplostomula (N. leei) inoculated orally penetrated the intestinal wall, entered the peritoneal cavity, and oriented to the liver without passing through any other organ. In rodent livers, the neodiplostomula were surrounded by host inflammatory cells and fibrotic tissues from day 10 postinfection (PI); the worms were found dead by day 56 PI. When neodiplostomula from rodent livers were transferred orally to mammals, they reoriented to the liver, although they were able to develop into adults in the chick intestine by day 6-7 PI. The possibility of human infections, i.e., liver migration, by N. leei neodiplostomula, among snake consumers, warrants investigation. 相似文献
967.
多花筋骨草是一种在江南广泛栽培的园艺植物,2005年秋,在杭州西湖风景区发现其黑胫病。该病害一年四季均可发生,发病最严重的是花期。黑胫病首先在寄主下部叶片的叶柄处发病,病斑初期为褐色、水渍状,很快变黑,最后整株枯萎死亡,典型症状是胫基部变黑腐烂。通过对病原菌形态学观察与核糖体DNA ITS序列分析,侵染多花筋骨草的6个分离菌株均被鉴定为多喙茎点霉Phoma multirostrata。致病性试验表明,多花筋骨草是多喙茎点霉的寄主。 相似文献
968.
969.
Sangwon Myoung James Lee Paul Constantino Peter Lucas Herzl Chai Brian Lawn 《Journal of biomechanics》2009,42(12):1947-1951
This study examines the inter-relation between enamel morphology and crack resistance by sectioning extracted human molars after loading to fracture. Cracks appear to initiate from tufts, hypocalcified defects at the enamel–dentin junction, and grow longitudinally around the enamel coat to produce failure. Microindentation corner cracks placed next to the tufts in the sections deflect along the tuft interfaces and occasionally penetrate into the adjacent enamel. Although they constitute weak interfaces, the tufts are nevertheless filled with organic matter, and appear to be stabilized against easy extension by self-healing, as well as by mutual stress-shielding and decussation, accounting at least in part for the capacity of tooth enamel to survive high functional forces. 相似文献
970.
Estabelle S.M. Ang Nathan J. Pavlos Lee Y. Chai Ming Qi Tak S. Cheng James H. Steer David A. Joyce Ming H. Zheng Jiake Xu 《Journal of cellular physiology》2009,221(3):642-649
Receptor activator NF‐κB ligand (RANKL)‐activated signaling is essential for osteoclast differentiation, activation and survival. Caffeic acid phenethyl ester (CAPE), a natural NF‐κB inhibitor from honeybee propolis has been shown to have anti‐tumor and anti‐inflammatory properties. In this study, we investigated the effect of CAPE on the regulation of RANKL‐induced osteoclastogenesis, bone resorption and signaling pathways. Low concentrations of CAPE (<1 µM) dose dependently inhibited RANKL‐induced osteoclastogenesis in RAW264.7 cell and bone marrow macrophage (BMM) cultures, as well as decreasing the capacity of human osteoclasts to resorb bone. CAPE inhibited both constitutive and RANKL‐induced NF‐κB and NFAT activation, concomitant with delayed IκBα degradation and inhibition of p65 nuclear translocation. At higher concentrations, CAPE induced apoptosis and caspase 3 activities of RAW264.7 and disrupts the microtubule network in osteoclast like (OCL) cells. Taken together, our findings demonstrate that inhibition of NF‐κB and NFAT activation by CAPE results in the attenuation of osteoclastogenesis and bone resorption, implying that CAPE is a potential treatment for osteolytic bone diseases. J. Cell. Physiol. 221: 642–649, 2009. © 2009 Wiley‐Liss, Inc. 相似文献