全文获取类型
收费全文 | 15319篇 |
免费 | 1446篇 |
国内免费 | 4篇 |
出版年
2023年 | 81篇 |
2022年 | 150篇 |
2021年 | 434篇 |
2020年 | 265篇 |
2019年 | 297篇 |
2018年 | 346篇 |
2017年 | 302篇 |
2016年 | 488篇 |
2015年 | 846篇 |
2014年 | 906篇 |
2013年 | 993篇 |
2012年 | 1333篇 |
2011年 | 1410篇 |
2010年 | 879篇 |
2009年 | 738篇 |
2008年 | 956篇 |
2007年 | 944篇 |
2006年 | 891篇 |
2005年 | 718篇 |
2004年 | 785篇 |
2003年 | 683篇 |
2002年 | 674篇 |
2001年 | 136篇 |
2000年 | 77篇 |
1999年 | 110篇 |
1998年 | 150篇 |
1997年 | 86篇 |
1996年 | 75篇 |
1995年 | 77篇 |
1994年 | 78篇 |
1993年 | 96篇 |
1992年 | 59篇 |
1991年 | 54篇 |
1990年 | 47篇 |
1989年 | 34篇 |
1988年 | 37篇 |
1987年 | 38篇 |
1986年 | 28篇 |
1985年 | 35篇 |
1984年 | 43篇 |
1983年 | 27篇 |
1982年 | 44篇 |
1981年 | 36篇 |
1980年 | 33篇 |
1979年 | 22篇 |
1978年 | 33篇 |
1977年 | 17篇 |
1976年 | 21篇 |
1975年 | 15篇 |
1974年 | 15篇 |
排序方式: 共有10000条查询结果,搜索用时 156 毫秒
941.
942.
943.
944.
Bryan D. Bryson Amanda M. Del Rosario Jonathan S. Gootenberg Michael B. Yaffe Forest M. White 《Proteomics》2015,15(9):1470-1475
MS‐based analysis of the acetylproteome has highlighted a role for acetylation in a wide array of biological processes including gene regulation, metabolism, and cellular signaling. To date, anti‐acetyllysine antibodies have been used as the predominant affinity reagent for enrichment of acetyllysine‐containing peptides and proteins; however, these reagents suffer from high nonspecific binding and lot‐to‐lot variability. Bromodomains represent potential affinity reagents for acetylated proteins and peptides, given their natural role in recognition of acetylated sequence motifs in vivo. To evaluate their efficacy, we generated recombinant proteins representing all known yeast bromodomains. Bromodomain specificity for acetylated peptides was determined using degenerate peptide arrays, leading to the observation that different bromodomains display a wide array of binding specificities. Despite their relatively weak affinity, we demonstrate the ability of selected bromodomains to enrich acetylated peptides from a complex biological mixture prior to mass spectrometric analysis. Finally, we demonstrate a method for improving the utility of bromodomain enrichment for MS through engineering novel affinity reagents using combinatorial tandem bromodomain pairs. 相似文献
945.
946.
Many cell types can bias their direction of locomotion by coupling to external cues. Characteristics such as how fast a cell migrates and the directedness of its migration path can be quantified to provide metrics that determine which biochemical and biomechanical factors affect directional cell migration, and by how much. To be useful, these metrics must be reproducible from one experimental setting to another. However, most are not reproducible because their numerical values depend on technical parameters like sampling interval and measurement error. To address the need for a reproducible metric, we analytically derive a metric called directionality time, the minimum observation time required to identify motion as directionally biased. We show that the corresponding fit function is applicable to a variety of ergodic, directionally biased motions. A motion is ergodic when the underlying dynamical properties such as speed or directional bias do not change over time. Measuring the directionality of nonergodic motion is less straightforward but we also show how this class of motion can be analyzed. Simulations are used to show the robustness of directionality time measurements and its decoupling from measurement errors. As a practical example, we demonstrate the measurement of directionality time, step-by-step, on noisy, nonergodic trajectories of chemotactic neutrophils. Because of its inherent generality, directionality time ought to be useful for characterizing a broad range of motions including intracellular transport, cell motility, and animal migration. 相似文献
947.
Jonathan C. Hill Elaine Thomas Susan Hill Nadine E. Foster Danielle A. van der Windt 《PloS one》2015,10(4)
Objective
To develop and validate a patient report outcome measure (PROM) for clinical practice that can monitor health status of patients with a range of musculoskeletal (MSK) disorders.Methods
Constructs for inclusion in the MSK-PROM were identified from a consensus process involving patients with musculoskeletal conditions, clinicians, purchasers of healthcare services, and primary care researchers. Psychometric properties of the brief tool, including face and construct validity, repeatability and responsiveness were assessed in a sample of patients with musculoskeletal pain consulting physiotherapy services in the United Kingdom (n=425).Results
The consensus process identified 10 prioritised domains for monitoring musculoskeletal health status: pain intensity, quality of life, physical capacity, interference with social/leisure activities, emotional well-being, severity of most difficult thing, activities and roles, understanding independence, and overall impact. As the EuroQol (EQ-5D-5L) is a widely adopted PROMs tool and covers the first four domains listed, to reduce patient burden to a minimum the MSK-PROM was designed to capture the remaining six prioritised domains which are not measured by the EQ-5D-5L. The tool demonstrated excellent reliability, construct validity, responsiveness and acceptability to patients and clinicians for use in clinical practice.Conclusion
We have validated a brief patient reported outcome measure (MSK-PROM) for use in clinical practice to measure musculoskeletal health status and monitor outcomes over time using domains that are meaningful to patients and sensitive to change. Further work will establish whether the MSK-PROM is useful in other musculoskeletal healthcare settings. 相似文献948.
Among the terpenes, isoprene (C5) and monoterpene hydrocarbons (C10) have been shown to ameliorate abiotic stress in a number of plant species via two proposed mechanisms: membrane stabilization and direct antioxidant effects. Sesquiterpene hydrocarbons (C15) not only share the structural properties thought to lend protective qualities to isoprene and monoterpene hydrocarbons, but also react rapidly with ozone, suggesting that sesquiterpenes may similarly enhance tolerance of abiotic stresses. To test whether sesquiterpenes protect plants against ozone, UVB light, or drought, we used transgenic lines of the wild tobacco Nicotiana attenuata. The transgenic plants expressed a maize terpene synthase gene (ZmTPS10) which produced a blend of (E)-ß-farnesene and (E)-α-bergamotene, or a point mutant of the same gene (ZmTPS10M) which produced (E)-ß-farnesene alone,. (E)-ß-farnesene exerted a local, external, and transient ozone-quenching effect in ozone-fumigated chambers, but we found no evidence that enhanced sesquiterpene production by the plant inhibited oxidative damage, or maintained photosynthetic function or plant fitness under acute or chronic stress. Although the sesquiterpenes (E)-ß-farnesene and (E)-α-bergamotene might confer benefits under intermittent heat stress, which was not tested, any roles in relieving abiotic stress may be secondary to their previously demonstrated functions in biotic interactions. 相似文献
949.
950.
Prini Mahendran Suneeta Soni Stephanie Goubet Emma Saunsbury Jonathan Roberts Martin Fisher 《PloS one》2015,10(4)